Restriction map for
pFN21AB7033
(Circular) (Six-base) MAPSORT of: pFN21AB7033.seq Check: 8116 from: 1 to: 5977
pFN21AB7033 5977 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2254 2790 2254
Size: 536 5441
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 3276 4104 4477 1563
Size: 1713 828 373 3063
Fragments arranged by size:
3063 1713 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4040 5424 5692 1632
Size: 2408 1384 268 1917
Fragments arranged by size:
2408 1917 1384 268
AfeI AGC'GCT
Cuts at: 2909 2975 2909
Size: 66 5911
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 2299 2913 4876 5853 1203
Size: 1096 614 1963 977 1327
Fragments arranged by size:
1963 1327 1096 977 614
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 2075 2148 2891 2994 1166
Size: 909 73 743 103 4149
Fragments arranged by size:
4149 909 743 103 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2909 1184
Size: 1725 4252
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 2372
Size: 32 5945
AlwNI CAG_nnn'CTG
Cuts at: 2530 3097 3130 3185 3206 3720 5292 2530
Size: 567 33 55 21 514 1572 3215
Fragments arranged by size:
3215 1572 567 514 55 33 21
ApaI G_GGCC'C
Cuts at: 3146 3146
Size: 5977
ApaLI G'TGCA_C
Cuts at: 2303 2542 4034 5190 2303
Size: 239 1492 1156 3090
Fragments arranged by size:
3090 1492 1156 239
ApoI r'AATT_y
Cuts at: 1493 2639 3286 3512 3664 5773 1493
Size: 1146 647 226 152 2109 1697
Fragments arranged by size:
2109 1697 1146 647 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2813 3302 1870
Size: 73 870 489 4545
Fragments arranged by size:
4545 870 489 73
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 2787 3171 3298 4753
Size: 325 217 627 355 645 384 127 1455
Cuts at: 4753 618
Size: 1842
Fragments arranged by size:
1842 1455 645 627 384 355 325 217
127
BanII G_rGCy'C
Cuts at: 1391 2607 3146 3296 1391
Size: 1216 539 150 4072
Fragments arranged by size:
4072 1216 539 150
BbeI G_GCGC'C
Cuts at: 3175 3175
Size: 5977
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5006
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4168 4202 1903
Size: 34 50 34 2147 34 3678
Fragments arranged by size:
3678 2147 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 3116 3164
Size: 107 122 71 631 605 454 990 48
Cuts at: 3164 3180 4588 136
Size: 16 1408 1525
Fragments arranged by size:
1525 1408 990 631 605 454 122 107
71 48 16
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 2025 2156 2237 3135 3394 2025
Size: 131 81 898 259 4608
Fragments arranged by size:
4608 898 259 131 81
Bme1580I G_kGCm'C
Cuts at: 1790 2307 2546 3146 4038 5194 1790
Size: 517 239 600 892 1156 2573
Fragments arranged by size:
2573 1156 892 600 517 239
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2581 2795 3002 3162 4637 1239
Size: 1064 278 214 207 160 1475 2579
Fragments arranged by size:
2579 1475 1064 278 214 207 160
Bpu10I CC'TnA_GC
Cuts at: 2049 2049
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2591 2825 4102 4967 5265 2492
Size: 99 234 1277 865 298 3204
Fragments arranged by size:
3204 1277 865 298 234 99
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2341 2521 3220 3768 493
Size: 711 1137 180 699 548 2702
Fragments arranged by size:
2702 1137 711 699 548 180
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2629 3172 3784 4166
Size: 53 83 186 936 1096 543 612 382
Cuts at: 4166 4801 275
Size: 635 1451
Fragments arranged by size:
1451 1096 936 635 612 543 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2594 2624 2903 2933 1178
Size: 30 165 30 1191 30 279 30 4222
Fragments arranged by size:
4222 1191 279 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 2828 2831 1186
Size: 1114 528 3 4332
Fragments arranged by size:
4332 1114 528 3
BseYI C'CCAG_C
Cuts at: 2882 3145 5180 5676 2882
Size: 263 2035 496 3183
Fragments arranged by size:
3183 2035 496 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 2282 2330 2543 3023 1163
Size: 573 528 18 48 213 480 4117
Fragments arranged by size:
4117 573 528 480 213 48 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2472 3427 4188 5216 1540
Size: 275 186 471 955 761 1028 2301
Fragments arranged by size:
2301 1028 955 761 471 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2546 2607 2699 2781 2921 3062 4038 4123
Size: 239 61 92 82 140 141 976 85
Cuts at: 4123 5194 2307
Size: 1071 3090
Fragments arranged by size:
3090 1071 976 239 141 140 92 85
82 61
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
BsmBI CGTCTCn'nnnn_
Cuts at: 2620 2620
Size: 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2546 2607 2699 2781 2921 3062
Size: 399 517 239 61 92 82 140 141
Cuts at: 3062 3146 3296 4038 4123 5194 1391
Size: 84 150 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 239 150 141
140 92 85 84 82 61
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3072 3866 5596 3072
Size: 794 1730 3453
Fragments arranged by size:
3453 1730 794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2254 2409 2790 3332 844
Size: 1410 155 381 542 3489
Fragments arranged by size:
3489 1410 542 381 155
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 2589 3864 1394
Size: 429 196 570 1275 3507
Fragments arranged by size:
3507 1275 570 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 3105 4472 4646 65
Size: 1183 1857 1367 174 1396
Fragments arranged by size:
1857 1396 1367 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2467 2471 4621 4808 1700
Size: 110 657 4 2150 187 2869
Fragments arranged by size:
2869 2150 657 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 2896 1925
Size: 971 5006
BssSI C'ACGA_G
Cuts at: 3080 4037 5049 3080
Size: 957 1012 4008
Fragments arranged by size:
4008 1012 957
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2260 2311 3066 794
Size: 1466 51 755 3705
Fragments arranged by size:
3705 1466 755 51
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2437 2996 2077
Size: 360 559 5058
Fragments arranged by size:
5058 559 360
BstYI r'GATC_y
Cuts at: 1972 2374 2820 3307 3674 4059 4076 5528 5614
Size: 402 446 487 367 385 17 1452 86
Cuts at: 5614 5626 5972 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 487 446 402 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2070 2082 513
Size: 552 1005 12 4408
Fragments arranged by size:
4408 1005 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2782 487
Size: 683 56 1556 3682
Fragments arranged by size:
3682 1556 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 2442 3035 3153 3493 4287 4307 2413
Size: 29 593 118 340 794 20 4083
Fragments arranged by size:
4083 794 593 340 118 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DraIII CAC_nnn'GTG
Cuts at: 2311 3046 3066 2311
Size: 735 20 5222
Fragments arranged by size:
5222 735 20
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2079 2162
Size: 54 1425 50 275 18 237 12 83
Cuts at: 2162 2469 2689 3424 4313 5942 8
Size: 307 220 735 889 1629 43
Fragments arranged by size:
1629 1425 889 735 307 275 237 220
83 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2469 3424 1537
Size: 275 657 955 4090
Fragments arranged by size:
4090 955 657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2193 3907 1353
Size: 840 1714 3423
Fragments arranged by size:
3423 1714 840
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2605 2605
Size: 5977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2581 2795 3002 3162 4040 4637
Size: 393 671 278 214 207 160 878 597
Cuts at: 4637 5424 5692 1239
Size: 787 268 1524
Fragments arranged by size:
1524 878 787 671 597 393 278 268
214 207 160
EcoO109I rG'GnC_Cy
Cuts at: 2770 3142 2770
Size: 372 5605
EcoRI G'AATT_C
Cuts at: 2639 3286 3664 2639
Size: 647 378 4952
Fragments arranged by size:
4952 647 378
FspI TGC'GCA
Cuts at: 2405 4483 2405
Size: 2078 3899
HaeII r_GCGC'y
Cuts at: 1678 2911 2977 3088 3122 3175 5124 1678
Size: 1233 66 111 34 53 1949 2531
Fragments arranged by size:
2531 1949 1233 111 66 53 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 4618 4650 4692
Size: 32 135 32 536 32 2478 32 42
Cuts at: 4692 4724 1373
Size: 32 2626
Fragments arranged by size:
2626 2478 536 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3052 3321 3567 669
Size: 988 281 1114 269 246 3079
Fragments arranged by size:
3079 1114 988 281 269 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KasI G'GCGC_C
Cuts at: 3171 3171
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 2604 5091 5275 1305
Size: 687 612 2487 184 2007
Fragments arranged by size:
2487 2007 687 612 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 2164 2691 10
Size: 54 1425 343 249 83 527 3296
Fragments arranged by size:
3296 1425 527 343 249 83 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2148 2708 2891 2994 3935 4294
Size: 648 909 73 560 183 103 941 359
Cuts at: 4294 4453 518
Size: 159 2042
Fragments arranged by size:
2042 941 909 648 560 359 183 159
103 73
MspA1I CmG'CkG
Cuts at: 1606 2330 2425 2559 2959 3149 3393 4070 5218
Size: 724 95 134 400 190 244 677 1148
Cuts at: 5218 5463 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 724 677 400 245 244 190
134 95
NaeI GCC'GGC
Cuts at: 2469 2469
Size: 5977
NarI GG'CG_CC
Cuts at: 3172 3172
Size: 5977
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5425
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NgoMIV G'CCGG_C
Cuts at: 2467 2467
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2303 2917 3335 4880 1132
Size: 97 1074 614 418 1545 2229
Fragments arranged by size:
2229 1545 1074 614 418 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 2299 2913 4876 2299
Size: 614 1963 3400
Fragments arranged by size:
3400 1963 614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2857 3097 1108
Size: 1749 240 3988
Fragments arranged by size:
3988 1749 240
PfoI T'CCnGG_A
Cuts at: 2667 2667
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PmlI CAC'GTG
Cuts at: 2341 2341
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 3142 3142
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2907 3126 3329 830
Size: 2077 219 203 3478
Fragments arranged by size:
3478 2077 219 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2330 2425 3149 2330
Size: 95 724 5158
Fragments arranged by size:
5158 724 95
RsrII CG'GwC_CG
Cuts at: 2554 2554
Size: 5977
SacI G_AGCT'C
Cuts at: 2607 2607
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SapI GCTCTTCn'nnn_
Cuts at: 2193 2193
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 2846 4225 5937 2846
Size: 1379 1712 2886
Fragments arranged by size:
2886 1712 1379
SexAI A'CCwGG_T
Cuts at: 2382 2946 2382
Size: 564 5413
SfcI C'TryA_G
Cuts at: 826 1045 2903 3122 3325 4460 5141 5332 826
Size: 219 1858 219 203 1135 681 191 1471
Fragments arranged by size:
1858 1471 1135 681 219 219 203 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2126 2126
Size: 5977
SfoI GGC'GCC
Cuts at: 3173 3173
Size: 5977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2507 2606 2840 4081 4982 5244
Size: 197 926 564 99 234 1241 901 262
Cuts at: 5244 820
Size: 1553
Fragments arranged by size:
1553 1241 926 901 564 262 234 197
99
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 2120 2720 1517
Size: 603 600 4774
Fragments arranged by size:
4774 603 600
StyI C'CwwG_G
Cuts at: 513 1065 2430 3416 513
Size: 552 1365 986 3074
Fragments arranged by size:
3074 1365 986 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2742 4004 4021 4174 4359 4804 325
Size: 1255 1162 1262 17 153 185 445 1498
Fragments arranged by size:
1498 1262 1255 1162 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2844 4223 5935
Size: 274 80 33 51 1443 867 1379 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
1712 1443 1379 867 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2286 2318 2794 2826 5937 5969 2286
Size: 32 476 32 3111 32 2294
Fragments arranged by size:
3111 2294 476 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3160 3160
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PpuMI PspXI PsrI SacII SanDI SrfI SwaI