Restriction map for
pFN21AB7032
(Circular) (Six-base) MAPSORT of: pFN21AB7032.seq Check: 8572 from: 1 to: 5977
pFN21AB7032 5977 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3223 3298 3223
Size: 75 5902
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2733 4040 5424 5692 1632
Size: 1101 1307 1384 268 1917
Fragments arranged by size:
1917 1384 1307 1101 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 2195 1166
Size: 1029 4948
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2273 1184
Size: 1089 4888
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 5945
AlwNI CAG_nnn'CTG
Cuts at: 2852 3720 5292 2852
Size: 868 1572 3537
Fragments arranged by size:
3537 1572 868
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 3026 3136 3286 3512 3664 5773 1493
Size: 1533 110 150 226 152 2109 1697
Fragments arranged by size:
2109 1697 1533 226 152 150 110
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3069 3302 1870
Size: 73 1126 233 4545
Fragments arranged by size:
4545 1126 233 73
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 3223 3298 4753 618
Size: 325 217 627 424 1012 75 1455 1842
Fragments arranged by size:
1842 1455 1012 627 424 325 217 75
BanII G_rGCy'C
Cuts at: 1391 3296 1391
Size: 1905 4072
BbeI G_GCGC'C
Cuts at: 2215 2215
Size: 5977
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5006
BbvCI CC'TCA_GC
Cuts at: 2949 2949
Size: 5977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 2505 2661 2799 1548
Size: 957 156 138 4726
Fragments arranged by size:
4726 957 156 138
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4588 136
Size: 107 122 71 631 605 337 2579 1525
Fragments arranged by size:
2579 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 3070 4038 5194 1790
Size: 1280 968 1156 2573
Fragments arranged by size:
2573 1280 1156 968
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2111
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2471 3231 4637 1239
Size: 1232 760 1406 2579
Fragments arranged by size:
2579 1406 1232 760
Bpu10I CC'TnA_GC
Cuts at: 2550 2949 2550
Size: 399 5578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 4967 5265 4102
Size: 865 298 4814
Fragments arranged by size:
4814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2082 4640 882
Size: 617 583 2558 2219
Fragments arranged by size:
2558 2219 617 583
BsaAI yAC'GTr
Cuts at: 493 1204 2812 3768 493
Size: 711 1608 956 2702
Fragments arranged by size:
2702 1608 956 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2212 3784 4166 4801
Size: 53 83 186 936 679 1572 382 635
Cuts at: 4801 275
Size: 1451
Fragments arranged by size:
1572 1451 936 679 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5752
Fragments arranged by size:
5752 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2966 3196 1186
Size: 862 918 230 3967
Fragments arranged by size:
3967 918 862 230
BseYI C'CCAG_C
Cuts at: 5180 5676 5180
Size: 496 5481
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3082 1163
Size: 573 1346 4058
Fragments arranged by size:
4058 1346 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4038 4123 5194 4038
Size: 85 1071 4821
Fragments arranged by size:
4821 1071 85
BsmI GAATG_Cn'
Cuts at: 2462 3488 3581 2462
Size: 1026 93 4858
Fragments arranged by size:
4858 1026 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3070 3296 4038 4123 5194 1391
Size: 399 1280 226 742 85 1071 2174
Fragments arranged by size:
2174 1280 1071 742 399 226 85
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 2538 3866 5596 2538
Size: 1328 1730 2919
Fragments arranged by size:
2919 1730 1328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 3332 844
Size: 1232 1256 3489
Fragments arranged by size:
3489 1256 1232
BsrBI CCG'CTC
Cuts at: 1394 1823 2124 3864 1394
Size: 429 301 1740 3507
Fragments arranged by size:
3507 1740 429 301
BsrDI GCAATG_nn'
Cuts at: 65 1248 2943 4472 4646 65
Size: 1183 1695 1529 174 1396
Fragments arranged by size:
1695 1529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 2048 1925
Size: 123 5854
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2622 794
Size: 1828 4149
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 2642 1184
Size: 1458 4519
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 2955 3307 3674 4059 4076 5528 5614 5626
Size: 983 352 367 385 17 1452 86 12
Cuts at: 5626 5972 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 983 385 367 352 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2648 2648
Size: 5977
BtgI C'CryG_G
Cuts at: 513 1065 2296 3056 513
Size: 552 1231 760 3434
Fragments arranged by size:
3434 1231 760 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 2098 487
Size: 683 56 812 60 4366
Fragments arranged by size:
4366 812 683 60 56
BtsI GCAGTG_nn'
Cuts at: 3070 3493 4287 4307 3070
Size: 423 794 20 4740
Fragments arranged by size:
4740 794 423 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DraIII CAC_nnn'GTG
Cuts at: 2361 2361
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2876 4984 809
Size: 507 1560 2108 1802
Fragments arranged by size:
2108 1802 1560 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2466 2859
Size: 54 1425 50 275 18 180 456 393
Cuts at: 2859 3424 4313 5942 8
Size: 565 889 1629 43
Fragments arranged by size:
1629 1425 889 565 456 393 275 180
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 2405 3907 1353
Size: 914 138 1502 3423
Fragments arranged by size:
3423 1502 914 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2471 2733 3231 4040 4637 5424 5692
Size: 393 839 262 498 809 597 787 268
Cuts at: 5692 1239
Size: 1524
Fragments arranged by size:
1524 839 809 787 597 498 393 268
262
EcoO109I rG'GnC_Cy
Cuts at: 2127 2127
Size: 5977
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 5977
FspI TGC'GCA
Cuts at: 2072 2681 4483 2072
Size: 609 1802 3566
Fragments arranged by size:
3566 1802 609
FspAI rTGC'GCAy
Cuts at: 2681 2681
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2215 5124 1678
Size: 537 2909 2531
Fragments arranged by size:
2909 2531 537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4618 4650 4692 4724 1373
Size: 32 135 32 3046 32 42 32 2626
Fragments arranged by size:
3046 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KasI G'GCGC_C
Cuts at: 2211 2211
Size: 5977
KpnI G_GTAC'C
Cuts at: 3227 3302 3227
Size: 75 5902
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5091 5275 1305
Size: 3786 184 2007
Fragments arranged by size:
3786 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 2468 2861 10
Size: 54 1425 343 180 456 393 3126
Fragments arranged by size:
3126 1425 456 393 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 2537 2687 3935 4294 4453 518
Size: 648 1029 342 150 1248 359 159 2042
Fragments arranged by size:
2042 1248 1029 648 359 342 159 150
MspA1I CmG'CkG
Cuts at: 1606 2253 3393 4070 5218 5463 1606
Size: 647 1140 677 1148 245 2120
Fragments arranged by size:
2120 1148 1140 677 647 245
NarI GG'CG_CC
Cuts at: 2212 2212
Size: 5977
NcoI C'CATG_G
Cuts at: 513 1065 2296 513
Size: 552 1231 4194
Fragments arranged by size:
4194 1231 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 3335 4880 1132
Size: 97 2106 1545 2229
Fragments arranged by size:
2229 2106 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 2872 1108
Size: 1113 651 4213
Fragments arranged by size:
4213 1113 651
PfoI T'CCnGG_A
Cuts at: 2028 2028
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PmlI CAC'GTG
Cuts at: 2812 2812
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2372 1613
Size: 759 5218
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2252 3329 830
Size: 1422 1077 3478
Fragments arranged by size:
3478 1422 1077
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2253 2253
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SapI GCTCTTCn'nnn_
Cuts at: 2267 2267
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2174 2248 3325 4460 5141 5332 826
Size: 219 1129 74 1077 1135 681 191 1471
Fragments arranged by size:
1471 1135 1129 1077 681 219 191 74
SfoI GGC'GCC
Cuts at: 2213 2213
Size: 5977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4081 4982 5244 820
Size: 197 926 2138 901 262 1553
Fragments arranged by size:
2138 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 2494 3901 5
Size: 47 2442 1407 2081
Fragments arranged by size:
2442 2081 1407 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2193 2296 3416 513
Size: 552 1128 103 1120 3074
Fragments arranged by size:
3074 1128 1120 552 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2630 4004 4021 4174 4359 4804 325
Size: 1255 1050 1374 17 153 185 445 1498
Fragments arranged by size:
1498 1374 1255 1050 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4223 5935 96
Size: 274 80 33 51 1443 2246 1712 138
Fragments arranged by size:
2246 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2869 2869
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 3172 4106 3172
Size: 934 5043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI NaeI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI