Restriction map for
pFN21AB7031
(Circular) (Six-base) MAPSORT of: pFN21AB7031.seq Check: 8864 from: 1 to: 5824
pFN21AB7031 5824 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2142 3167 1937
Size: 205 1025 4594
Fragments arranged by size:
4594 1025 205
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2566 2869 3887 5271 5539 1632
Size: 934 303 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1018 934 303 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 2370 4723 5700 1203
Size: 1167 2353 977 1327
Fragments arranged by size:
2353 1327 1167 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 2732 1166
Size: 1566 4258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2441 1184
Size: 1257 4567
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 3149 1870
Size: 73 219 987 4545
Fragments arranged by size:
4545 987 219 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2823 3145 4600 618
Size: 325 217 627 1036 322 1455 1842
Fragments arranged by size:
1842 1455 1036 627 325 322 217
BanII G_rGCy'C
Cuts at: 1391 2856 3143 1391
Size: 1465 287 4072
Fragments arranged by size:
4072 1465 287
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2222 2266 2377 2524 2595 2875 928
Size: 971 323 44 111 147 71 280 3877
Fragments arranged by size:
3877 971 323 280 147 111 71 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 2187 1548
Size: 639 5185
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2828 3885 5041 1790
Size: 1038 1057 1156 2573
Fragments arranged by size:
2573 1156 1057 1038
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2326 2693 3555
Size: 416 53 83 399 24 909 367 862
Cuts at: 3555 4513 442
Size: 958 1753
Fragments arranged by size:
1753 958 909 862 416 399 367 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4484 1239
Size: 3245 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2533 3949 4814 5112 2533
Size: 1416 865 298 3245
Fragments arranged by size:
3245 1416 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3104 4487 882
Size: 617 1605 1383 2219
Fragments arranged by size:
2219 1605 1383 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2829 2859 1178
Size: 30 165 30 1426 30 4143
Fragments arranged by size:
4143 1426 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 1186
Size: 1234 4590
BseYI C'CCAG_C
Cuts at: 3047 5027 5523 3047
Size: 1980 496 3348
Fragments arranged by size:
3348 1980 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5251
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2430 2856 3885 3970 5041 2430
Size: 426 1029 85 1071 3213
Fragments arranged by size:
3213 1071 1029 426 85
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 2828 2856 3143 3885 3970 5041
Size: 399 640 398 28 287 742 85 1071
Cuts at: 5041 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 640 399 398 287 85
28
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2559 3179 844
Size: 1715 620 3489
Fragments arranged by size:
3489 1715 620
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2182 2193 2919 4319 4493 65
Size: 1183 934 11 726 1400 174 1396
Fragments arranged by size:
1400 1396 1183 934 726 174 11
BsrFI r'CCGG_y
Cuts at: 1700 1810 2649 3113 4468 4655 1700
Size: 110 839 464 1355 187 2869
Fragments arranged by size:
2869 1355 839 464 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 2608 3884 4896 2608
Size: 1276 1012 3536
Fragments arranged by size:
3536 1276 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2154 794
Size: 1360 4464
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 2116 1184
Size: 932 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 2124 3154 3521 3906 3923 5375 5461 5473
Size: 152 1030 367 385 17 1452 86 12
Cuts at: 5473 5819 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1030 385 367 346 152 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 4033
Fragments arranged by size:
4033 1239 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 2256 2862 2907 3340 4134 4154 2256
Size: 606 45 433 794 20 3926
Fragments arranged by size:
3926 794 606 433 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2896 2931 529
Size: 35 2332 35 3422
Fragments arranged by size:
3422 2332 35 35
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 2659 2659
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2760 2904 3271
Size: 54 1425 50 275 18 930 144 367
Cuts at: 3271 4160 5789 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 930 889 367 275 144 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2999 3043 3754 1353
Size: 1646 44 711 3423
Fragments arranged by size:
3423 1646 711 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2854 2854
Size: 5824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2566 2869 3887 4484 5271 5539 1239
Size: 393 934 303 1018 597 787 268 1524
Fragments arranged by size:
1524 1018 934 787 597 393 303 268
EcoO109I rG'GnC_Cy
Cuts at: 2497 2497
Size: 5824
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2794 2794
Size: 5824
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 4971 1678
Size: 3293 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 4465 4497 4539
Size: 32 135 32 509 32 2352 32 42
Cuts at: 4539 4571 1373
Size: 32 2626
Fragments arranged by size:
2626 2352 509 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3058 748
Size: 2310 3514
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2762 2906 10
Size: 54 1425 343 930 144 2928
Fragments arranged by size:
2928 1425 930 343 144 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2423 2660 2732 2867 3782 4141
Size: 648 843 414 237 72 135 915 359
Cuts at: 4141 4300 518
Size: 159 2042
Fragments arranged by size:
2042 915 843 648 414 359 237 159
135 72
MspA1I CmG'CkG
Cuts at: 1606 2415 2931 3240 3917 5065 5310 1606
Size: 809 516 309 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 677 516 309 245
NaeI GCC'GGC
Cuts at: 2651 2651
Size: 5824
NcoI C'CATG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 4033
Fragments arranged by size:
4033 1239 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2649 2649
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2374 3121 3182 4727 1132
Size: 97 1145 747 61 1545 2229
Fragments arranged by size:
2229 1545 1145 747 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 2370 4723 2370
Size: 2353 3471
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PfoI T'CCnGG_A
Cuts at: 2535 2535
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2497 2497
Size: 5824
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2748 3176 830
Size: 1918 428 3478
Fragments arranged by size:
3478 1918 428
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2931 2931
Size: 5824
SacI G_AGCT'C
Cuts at: 2856 2856
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2143 2744 3172 4307 4988 5179 826
Size: 219 1098 601 428 1135 681 191 1471
Fragments arranged by size:
1471 1135 1098 681 601 428 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2512 3928 4829 5091 820
Size: 197 926 569 1416 901 262 1553
Fragments arranged by size:
1553 1416 926 901 569 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3121 3182 1132
Size: 1989 61 3774
Fragments arranged by size:
3774 1989 61
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2103 2304 3263 513
Size: 552 1038 201 959 3074
Fragments arranged by size:
3074 1038 959 552 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 3868 4021 4206 4651 325
Size: 1255 2271 17 153 185 445 1498
Fragments arranged by size:
2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4070 5782 96
Size: 274 80 33 51 1443 2093 1712 138
Fragments arranged by size:
2093 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2829 2861 5784 5816 2829
Size: 32 2923 32 2837
Fragments arranged by size:
2923 2837 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2128 2083
Size: 45 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 2777 3953 2777
Size: 1176 4648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI