Restriction map for
pFN21AB7021
(Circular) (Six-base) MAPSORT of: pFN21AB7021.seq Check: 14 from: 1 to: 6349
pFN21AB7021 6349 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2352 2352
Size: 6349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 1937
Size: 1755 4594
Acc65I G'GTAC_C
Cuts at: 3362 3670 3362
Size: 308 6041
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 4412 5796 6064 1632
Size: 1172 1608 1384 268 1917
Fragments arranged by size:
1917 1608 1384 1172 268
AfeI AGC'GCT
Cuts at: 2092 2092
Size: 6349
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 5248 6225 1203
Size: 4045 977 1327
Fragments arranged by size:
4045 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 2576 3291 1166
Size: 1410 715 4224
Fragments arranged by size:
4224 1410 715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2866 1184
Size: 1682 4667
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 2237
Size: 32 6317
AlwNI CAG_nnn'CTG
Cuts at: 2455 2635 2689 2772 3112 3388 3526 3613 4092
Size: 180 54 83 340 276 138 87 479
Cuts at: 4092 5664 2455
Size: 1572 3140
Fragments arranged by size:
3140 1572 479 340 276 180 138 87
83 54
ApaI G_GGCC'C
Cuts at: 2033 2033
Size: 6349
ApaLI G'TGCA_C
Cuts at: 2846 4406 5562 2846
Size: 1560 1156 3633
Fragments arranged by size:
3633 1560 1156
ApoI r'AATT_y
Cuts at: 1493 3658 3884 4036 6145 1493
Size: 2165 226 152 2109 1697
Fragments arranged by size:
2165 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 3396 3543 3674 1870
Size: 73 89 1364 147 131 4545
Fragments arranged by size:
4545 1364 147 131 89 73
BamHI G'GATC_C
Cuts at: 2929 3679 4046 2929
Size: 750 367 5232
Fragments arranged by size:
5232 750 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2176 2581 3059 3081 3123
Size: 325 217 627 389 405 478 22 42
Cuts at: 3123 3196 3262 3362 3670 5125 618
Size: 73 66 100 308 1455 1842
Fragments arranged by size:
1842 1455 627 478 405 389 325 308
217 100 73 66 42 22
BanII G_rGCy'C
Cuts at: 1391 2033 2718 3404 3668 1391
Size: 642 685 686 264 4072
Fragments arranged by size:
4072 686 685 642 264
BbeI G_GCGC'C
Cuts at: 3063 3063
Size: 6349
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5378
BbvCI CC'TCA_GC
Cuts at: 3320 3320
Size: 6349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3524 3558 4540 4574 1903
Size: 34 1587 34 982 34 3678
Fragments arranged by size:
3678 1587 982 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4960 136
Size: 107 122 71 631 605 3288 1525
Fragments arranged by size:
3288 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3477 6344 3477
Size: 2867 3482
BlpI GC'TnA_GC
Cuts at: 2712 3766 2712
Size: 1054 5295
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2181 2383 2850 4410 5566 1790
Size: 243 148 202 467 1560 1156 2573
Fragments arranged by size:
2573 1560 1156 467 243 202 148
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2935 3461 4080
Size: 416 53 83 399 24 1518 526 619
Cuts at: 4080 5038 442
Size: 958 1753
Fragments arranged by size:
1753 1518 958 619 526 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2713 3161 5009 1239
Size: 1474 448 1848 2579
Fragments arranged by size:
2579 1848 1474 448
Bpu10I CC'TnA_GC
Cuts at: 3320 3320
Size: 6349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 4474 5339 5637 2393
Size: 2081 865 298 3105
Fragments arranged by size:
3105 2081 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5012 882
Size: 617 3513 2219
Fragments arranged by size:
3513 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2884 4140 493
Size: 711 1680 1256 2702
Fragments arranged by size:
2702 1680 1256 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 3060 3573 4156
Size: 53 83 186 936 578 949 513 583
Cuts at: 4156 4538 5173 275
Size: 382 635 1451
Fragments arranged by size:
1451 949 936 635 583 578 513 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4782 5180 5454 5601 6012 4782
Size: 398 274 147 411 5119
Fragments arranged by size:
5119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6124
Fragments arranged by size:
6124 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3531 1186
Size: 2345 4004
BseYI C'CCAG_C
Cuts at: 2126 2698 2754 2830 2895 3354 3566 3576 3593
Size: 572 56 76 65 459 212 10 17
Cuts at: 3593 5552 6048 2126
Size: 1959 496 2427
Fragments arranged by size:
2427 1959 572 496 459 212 76 65
56 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 2758 3098 3401 3554 1163
Size: 573 912 110 340 303 153 3958
Fragments arranged by size:
3958 912 573 340 303 153 110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 3799 4560 5588 1540
Size: 275 186 1300 498 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 498 275 186
BsiHKAI G_wGCw'C
Cuts at: 2622 2850 4410 4495 5566 2622
Size: 228 1560 85 1071 3405
Fragments arranged by size:
3405 1560 1071 228 85
BsmI GAATG_Cn'
Cuts at: 2227 3860 3953 2227
Size: 1633 93 4623
Fragments arranged by size:
4623 1633 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2181 2383 2622 2718 2850 3404
Size: 399 243 148 202 239 96 132 554
Cuts at: 3404 3668 4410 4495 5566 1391
Size: 264 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 399 264 243 239
202 148 132 96 85
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 2352 2454 2511 2643 3704 844
Size: 1352 156 102 57 132 1061 3489
Fragments arranged by size:
3489 1352 1061 156 132 102 57
BsrBI CCG'CTC
Cuts at: 1394 1823 2803 4236 1394
Size: 429 980 1433 3507
Fragments arranged by size:
3507 1433 980 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4844 5018 65
Size: 1183 3596 174 1396
Fragments arranged by size:
3596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3296 4993 5180 1700
Size: 110 1486 1697 187 2869
Fragments arranged by size:
2869 1697 1486 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2455 2626 2635 2980 3388 794
Size: 1661 171 9 345 408 3755
Fragments arranged by size:
3755 1661 408 345 171 9
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 3253 1184
Size: 2069 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 2903 2903
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 2238 2929 3345 3477 3637 3679 4046 4431
Size: 266 691 416 132 160 42 367 385
Cuts at: 4431 4448 5900 5986 5998 6344 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 691 416 385 367 346 266
160 132 86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2574 3036 3167 513
Size: 552 939 570 462 131 3695
Fragments arranged by size:
3695 939 570 552 462 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 2453 2709 2848 2850 3110 3463 3865 4659 4679
Size: 256 139 2 260 353 402 794 20
Cuts at: 4679 2453
Size: 4123
Fragments arranged by size:
4123 794 402 353 260 256 139 20
2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2653 2688 529
Size: 35 2089 35 4190
Fragments arranged by size:
4190 2089 35 35
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DraIII CAC_nnn'GTG
Cuts at: 2626 3631 2626
Size: 1005 5344
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2433 3298 3433
Size: 54 1425 50 275 18 603 865 135
Cuts at: 3433 3796 4685 6314 8
Size: 363 889 1629 43
Fragments arranged by size:
1629 1425 889 865 603 363 275 135
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 3796 1537
Size: 275 1486 498 4090
Fragments arranged by size:
4090 1486 498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 4279 1353
Size: 1007 1919 3423
Fragments arranged by size:
3423 1919 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4936 5310 5456 387
Size: 1064 138 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2713 2804 3161 4412 5009 5796 6064
Size: 393 1081 91 357 1251 597 787 268
Cuts at: 6064 1239
Size: 1524
Fragments arranged by size:
1524 1251 1081 787 597 393 357 268
91
EcoO109I rG'GnC_Cy
Cuts at: 2029 2100 2632 3385 2029
Size: 71 532 753 4993
Fragments arranged by size:
4993 753 532 71
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 6349
FspI TGC'GCA
Cuts at: 2984 4855 2984
Size: 1871 4478
HaeII r_GCGC'y
Cuts at: 1678 2054 2094 3063 5496 1678
Size: 376 40 969 2433 2531
Fragments arranged by size:
2531 2433 969 376 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4990 5022 5064 5096 1373
Size: 32 135 32 3418 32 42 32 2626
Fragments arranged by size:
3418 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KasI G'GCGC_C
Cuts at: 3059 3059
Size: 6349
KpnI G_GTAC'C
Cuts at: 3366 3674 3366
Size: 308 6041
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 5463 5647 1305
Size: 1014 3144 184 2007
Fragments arranged by size:
3144 2007 1014 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 10
Size: 54 1425 343 603 3924
Fragments arranged by size:
3924 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 3087 3291 3632 4307 4666 4825
Size: 648 1410 511 204 341 675 359 159
Cuts at: 4825 518
Size: 2042
Fragments arranged by size:
2042 1410 675 648 511 359 341 204
159
MspA1I CmG'CkG
Cuts at: 1606 2130 2269 2641 2878 2899 3078 3765 4442
Size: 524 139 372 237 21 179 687 677
Cuts at: 4442 5590 5835 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 524 372 245 237
179 139 21
NaeI GCC'GGC
Cuts at: 3298 3298
Size: 6349
NarI GG'CG_CC
Cuts at: 3060 3060
Size: 6349
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4858
Fragments arranged by size:
4858 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NgoMIV G'CCGG_C
Cuts at: 3296 3296
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 3562 3707 5252 1132
Size: 97 2333 145 1545 2229
Fragments arranged by size:
2333 2229 1545 145 97
PasI CC'CwG_GG
Cuts at: 1348 3244 1348
Size: 1896 4453
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2689 3526 1108
Size: 1581 837 3931
Fragments arranged by size:
3931 1581 837
PfoI T'CCnGG_A
Cuts at: 2241 2613 3519 2241
Size: 372 906 5071
Fragments arranged by size:
5071 906 372
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2177 2209 4418 4450 5931 5963 2177
Size: 32 2209 32 1481 32 2563
Fragments arranged by size:
2563 2209 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 6349
PshAI GACnn'nnGTC
Cuts at: 1613 2410 1613
Size: 797 5552
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 2029 2029
Size: 6349
PstI C_TGCA'G
Cuts at: 830 2277 2349 2451 2845 3417 3701 830
Size: 1447 72 102 394 572 284 3478
Fragments arranged by size:
3478 1447 572 394 284 102 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2130 2878 2899 2130
Size: 748 21 5580
Fragments arranged by size:
5580 748 21
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 2273 2345 2447 2841 3413 3697 4832
Size: 219 1228 72 102 394 572 284 1135
Cuts at: 4832 5513 5704 826
Size: 681 191 1471
Fragments arranged by size:
1471 1228 1135 681 572 394 284 219
191 102 72
SfoI GGC'GCC
Cuts at: 3061 3061
Size: 6349
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 2034 3398 3676 1872
Size: 162 1364 278 4545
Fragments arranged by size:
4545 1364 278 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 4453 5354 5616 820
Size: 197 926 465 2045 901 262 1553
Fragments arranged by size:
2045 1553 926 901 465 262 197
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2004 3051 3788 513
Size: 552 939 1047 737 3074
Fragments arranged by size:
3074 1047 939 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2599 3239 3419 3488 4376 4393 4546
Size: 1255 1019 640 180 69 888 17 153
Cuts at: 4546 4731 5176 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1019 888 640 445 185 180
153 69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4595 6307 96
Size: 274 80 33 51 1443 2618 1712 138
Fragments arranged by size:
2618 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6309 6341 6309
Size: 32 6317
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 2575
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 2032 3396 3674 1870
Size: 162 1364 278 4545
Fragments arranged by size:
4545 1364 278 162
XmnI GAAnn'nnTTC
Cuts at: 3092 4478 3092
Size: 1386 4963
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI