Restriction map for
pFN21AB7020
(Circular) (Six-base) MAPSORT of: pFN21AB7020.seq Check: 7461 from: 1 to: 7699
pFN21AB7020 7699 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2969 2732
Size: 237 7462
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5509 278
Size: 53 83 186 936 3973 2468
Fragments arranged by size:
3973 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2319 3955 5042 1937
Size: 382 1636 1087 4594
Fragments arranged by size:
4594 1636 1087 382
Acc65I G'GTAC_C
Cuts at: 5020 5020
Size: 7699
AclI AA'CG_TT
Cuts at: 1563 5826 6199 1563
Size: 4263 373 3063
Fragments arranged by size:
4263 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3387 4025 5762 7146 7414 1632
Size: 1755 638 1737 1384 268 1917
Fragments arranged by size:
1917 1755 1737 1384 638 268
AfeI AGC'GCT
Cuts at: 4726 4726
Size: 7699
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7502
AflIII A'CryG_T
Cuts at: 1203 3891 6598 7575 1203
Size: 2688 2707 977 1327
Fragments arranged by size:
2707 2688 1327 977
AgeI A'CCGG_T
Cuts at: 6530 6530
Size: 7699
AhdI GACnn_n'nnGTC
Cuts at: 6428 6428
Size: 7699
AleI CACnn'nnGTG
Cuts at: 1166 2534 3017 1166
Size: 1368 483 5848
Fragments arranged by size:
5848 1368 483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3035 3822 1184
Size: 1851 787 5061
Fragments arranged by size:
5061 1851 787
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 2318
Size: 32 7667
AlwNI CAG_nnn'CTG
Cuts at: 2955 3009 3590 4984 5442 7014 2955
Size: 54 581 1394 458 1572 3640
Fragments arranged by size:
3640 1572 1394 581 458 54
ApaI G_GGCC'C
Cuts at: 2904 3655 2904
Size: 751 6948
ApaLI G'TGCA_C
Cuts at: 4268 5756 6912 4268
Size: 1488 1156 5055
Fragments arranged by size:
5055 1488 1156
ApoI r'AATT_y
Cuts at: 1493 3932 4655 5008 5234 5386 7495 1493
Size: 2439 723 353 226 152 2109 1697
Fragments arranged by size:
2439 2109 1697 723 353 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6253 160
Size: 872 5221 1606
Fragments arranged by size:
5221 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7699
AvaI C'yCGr_G
Cuts at: 1870 1943 2672 3033 3074 3090 3298 4094 5024
Size: 73 729 361 41 16 208 796 930
Cuts at: 5024 1870
Size: 4545
Fragments arranged by size:
4545 930 796 729 361 208 73 41
16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2914 2947 2914
Size: 33 7666
BamHI G'GATC_C
Cuts at: 5029 5396 5029
Size: 367 7332
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 2720 2752 2794 2804
Size: 325 217 627 807 126 32 42 10
Cuts at: 2804 3424 5020 6475 618
Size: 620 1596 1455 1842
Fragments arranged by size:
1842 1596 1455 807 627 620 325 217
126 42 32 10
BanII G_rGCy'C
Cuts at: 1391 2017 2331 2697 2904 3090 3311 3655 3692
Size: 626 314 366 207 186 221 344 37
Cuts at: 3692 5018 1391
Size: 1326 4072
Fragments arranged by size:
4072 1326 626 366 344 314 221 207
186 37
BbeI G_GCGC'C
Cuts at: 2598 2598
Size: 7699
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3703 4899 928
Size: 971 1804 1196 3728
Fragments arranged by size:
3728 1804 1196 971
BbvCI CC'TCA_GC
Cuts at: 2431 3163 2431
Size: 732 6967
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4265 4299 5890 5924 1903
Size: 34 2328 34 1591 34 3678
Fragments arranged by size:
3678 2328 1591 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4961 5591 6801 4961
Size: 630 1210 5859
Fragments arranged by size:
5859 1210 630
BclI T'GATC_A
Cuts at: 1548 4072 1548
Size: 2524 5175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2047 4427 6310
Size: 107 122 71 631 605 375 2380 1883
Cuts at: 6310 136
Size: 1525
Fragments arranged by size:
2380 1883 1525 631 605 375 122 107
71
BglII A'GATC_T
Cuts at: 7694 7694
Size: 7699
BlpI GC'TnA_GC
Cuts at: 5116 5116
Size: 7699
Bme1580I G_kGCm'C
Cuts at: 1790 2904 3375 3427 3580 3655 4272 5760 6916
Size: 1114 471 52 153 75 617 1488 1156
Cuts at: 6916 1790
Size: 2573
Fragments arranged by size:
2573 1488 1156 1114 617 471 153 75
52
BmgBI CAC'GTC
Cuts at: 1318 3892 1318
Size: 2574 5125
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2694 2845 4531
Size: 416 53 83 399 24 1277 151 1686
Cuts at: 4531 5430 6388 442
Size: 899 958 1753
Fragments arranged by size:
1753 1686 1277 958 899 416 399 151
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7699
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2716 3661 2518
Size: 198 945 6556
Fragments arranged by size:
6556 945 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3174 3647 4373 4489 6359 1239
Size: 1935 473 726 116 1870 2579
Fragments arranged by size:
2579 1935 1870 726 473 116
Bpu10I CC'TnA_GC
Cuts at: 2431 3163 3397 4043 4943 2431
Size: 732 234 646 900 5187
Fragments arranged by size:
5187 900 732 646 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2744 4694 5824 6689 6987 2315
Size: 429 1950 1130 865 298 3027
Fragments arranged by size:
3027 1950 1130 865 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 2381 2669 3620 6362 882
Size: 617 618 264 288 951 2742 2219
Fragments arranged by size:
2742 2219 951 618 617 288 264
BsaAI yAC'GTr
Cuts at: 493 1204 2323 2467 5490 493
Size: 711 1119 144 3023 2702
Fragments arranged by size:
3023 2702 1119 711 144
BsaBI GATnn'nnATC
Cuts at: 4406 7693 4406
Size: 3287 4412
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2595 3552 5506 5888
Size: 53 83 186 936 1062 957 1954 382
Cuts at: 5888 6523 275
Size: 635 1451
Fragments arranged by size:
1954 1451 1062 957 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4638 6132 6530 6804 6951 7362 4638
Size: 1494 398 274 147 411 4975
Fragments arranged by size:
4975 1494 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 2837 2867 4734
Size: 30 165 30 915 30 489 30 1867
Cuts at: 4734 4764 1178
Size: 30 4113
Fragments arranged by size:
4113 1867 915 489 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2428 3926 1186
Size: 1242 1498 4959
Fragments arranged by size:
4959 1498 1242
BseYI C'CCAG_C
Cuts at: 2941 3211 3654 3701 3926 4626 6902 7398 2941
Size: 270 443 47 225 700 2276 496 3242
Fragments arranged by size:
3242 2276 700 496 443 270 225 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2330 2794 2941 2995 3003 3299 3371
Size: 573 594 464 147 54 8 296 72
Cuts at: 3371 3548 4589 5012 1163
Size: 177 1041 423 3850
Fragments arranged by size:
3850 1041 594 573 464 423 296 177
147 72 54 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2521 3333 5149 5910 6938 1540
Size: 275 186 520 812 1816 761 1028 2301
Fragments arranged by size:
2301 1816 1028 812 761 520 275 186
BsiHKAI G_wGCw'C
Cuts at: 2331 4272 5760 5845 6916 2331
Size: 1941 1488 85 1071 3114
Fragments arranged by size:
3114 1941 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 5210 5303 5210
Size: 93 7606
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2331 2697 2904 3090 3311 3375
Size: 399 227 314 366 207 186 221 64
Cuts at: 3375 3427 3580 3655 3692 4272 5018 5760 5845
Size: 52 153 75 37 580 746 742 85
Cuts at: 5845 6916 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 746 742 580 399 366 314
227 221 207 186 153 85 75 64
52 37
BspEI T'CCGG_A
Cuts at: 7362 7362
Size: 7699
BspHI T'CATG_A
Cuts at: 4497 5588 7318 4497
Size: 1091 1730 4878
Fragments arranged by size:
4878 1730 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 2969 4905 4984 5054 844
Size: 1888 237 1936 79 70 3489
Fragments arranged by size:
3489 1936 1888 237 79 70
BsrBI CCG'CTC
Cuts at: 1394 1823 3436 5586 1394
Size: 429 1613 2150 3507
Fragments arranged by size:
3507 2150 1613 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2481 2709 3804 4227 4702 4716 6194
Size: 1183 1233 228 1095 423 475 14 1478
Cuts at: 6194 6368 65
Size: 174 1396
Fragments arranged by size:
1478 1396 1233 1183 1095 475 423 228
174 14
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 2516 2822 4235 4569 4922 6343
Size: 110 292 414 306 1413 334 353 1421
Cuts at: 6343 6530 1700
Size: 187 2869
Fragments arranged by size:
2869 1421 1413 414 353 334 306 292
187 110
BsrGI T'GTAC_A
Cuts at: 96 3120 96
Size: 3024 4675
BssHII G'CGCG_C
Cuts at: 1925 2620 1925
Size: 695 7004
BssSI C'ACGA_G
Cuts at: 5759 6771 5759
Size: 1012 6687
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3141 794
Size: 2347 5352
BstBI TT'CG_AA
Cuts at: 6503 6503
Size: 7699
BstEII G'GTnAC_C
Cuts at: 1184 4105 4912 1184
Size: 2921 807 3971
Fragments arranged by size:
3971 2921 807
BstXI CCAn_nnnn'nTGG
Cuts at: 2704 3152 4390 4819 2704
Size: 448 1238 429 5584
Fragments arranged by size:
5584 1238 448 429
BstYI r'GATC_y
Cuts at: 1972 4401 4938 5029 5396 5781 5798 7250 7336
Size: 2429 537 91 367 385 17 1452 86
Cuts at: 7336 7348 7694 1972
Size: 12 346 1977
Fragments arranged by size:
2429 1977 1452 537 385 367 346 91
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3749 4646 3749
Size: 897 6802
BtgI C'CryG_G
Cuts at: 513 1065 2004 2739 3145 3949 4554 4584 513
Size: 552 939 735 406 804 605 30 3628
Fragments arranged by size:
3628 939 804 735 605 552 406 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2436 2793 3478 3718 487
Size: 683 56 1210 357 685 240 4468
Fragments arranged by size:
4468 1210 685 683 357 240 56
BtsI GCAGTG_nn'
Cuts at: 4601 5215 6009 6029 4601
Size: 614 794 20 6271
Fragments arranged by size:
6271 794 614 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7664
DraI TTT'AAA
Cuts at: 5003 5003
Size: 7699
DraIII CAC_nnn'GTG
Cuts at: 3179 3179
Size: 7699
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4763 6706 809
Size: 507 3447 1943 1802
Fragments arranged by size:
3447 1943 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2518 2824
Size: 54 1425 50 275 18 177 511 306
Cuts at: 2824 3103 3330 3970 3990 4118 4920 4924 5146
Size: 279 227 640 20 128 802 4 222
Cuts at: 5146 6035 7664 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 802 640 511 306 279
275 227 222 177 128 54 50 43
20 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2518 3330 5146 1537
Size: 275 706 812 1816 4090
Fragments arranged by size:
4090 1816 812 706 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 2187 2405 2672 3476 5629 1353
Size: 762 72 218 267 804 2153 3423
Fragments arranged by size:
3423 2153 804 762 267 218 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2705 6286 6660 6806 387
Size: 1064 138 1116 3581 374 146 1280
Fragments arranged by size:
3581 1280 1116 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2329 2329
Size: 7699
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3174 3387 3647 4025 4373 4489 5762
Size: 393 1542 213 260 378 348 116 1273
Cuts at: 5762 6359 7146 7414 1239
Size: 597 787 268 1524
Fragments arranged by size:
1542 1524 1273 787 597 393 378 348
268 260 213 116
EcoNI CCTnn'n_nnAGG
Cuts at: 4046 4046
Size: 7699
EcoO109I rG'GnC_Cy
Cuts at: 2900 2901 3094 3159 3651 3850 2900
Size: 1 193 65 492 199 6749
Fragments arranged by size:
6749 492 199 193 65 1
EcoRI G'AATT_C
Cuts at: 5008 5386 5008
Size: 378 7321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2584 4855 2407
Size: 177 2271 5251
Fragments arranged by size:
5251 2271 177
FseI GG_CCGG'CC
Cuts at: 4926 4926
Size: 7699
FspI TGC'GCA
Cuts at: 2540 6205 2540
Size: 3665 4034
HaeII r_GCGC'y
Cuts at: 1678 2117 2598 3229 4728 6846 1678
Size: 439 481 631 1499 2118 2531
Fragments arranged by size:
2531 2118 1499 631 481 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 2414 2446 2978
Size: 32 135 32 586 32 224 32 532
Cuts at: 2978 3010 3027 3059 4349 4381 6340 6372 6414
Size: 32 17 32 1290 32 1959 32 42
Cuts at: 6414 6446 1373
Size: 32 2626
Fragments arranged by size:
2626 1959 1290 586 532 224 135 42
32 32 32 32 32 32 32 32
32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 3289 3956 4303 5043 5289
Size: 988 281 589 762 667 347 740 246
Cuts at: 5289 669
Size: 3079
Fragments arranged by size:
3079 988 762 740 667 589 347 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7699
HpaI GTT'AAC
Cuts at: 5289 5289
Size: 7699
KasI G'GCGC_C
Cuts at: 2594 2594
Size: 7699
KpnI G_GTAC'C
Cuts at: 5024 5024
Size: 7699
MfeI C'AATT_G
Cuts at: 5298 5298
Size: 7699
MluI A'CGCG_T
Cuts at: 7575 7575
Size: 7699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6813 6997 1305
Size: 5508 184 2007
Fragments arranged by size:
5508 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3992 10
Size: 54 1425 343 177 1983 3717
Fragments arranged by size:
3717 1983 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2615 3017 4817 5657 6016 6175
Size: 648 1368 81 402 1800 840 359 159
Cuts at: 6175 518
Size: 2042
Fragments arranged by size:
2042 1800 1368 840 648 402 359 159
81
MspA1I CmG'CkG
Cuts at: 1606 2059 2071 2086 2314 2837 3211 3532 3701
Size: 453 12 15 228 523 374 321 169
Cuts at: 3701 4030 4207 4413 5115 5792 6940 7185 1606
Size: 329 177 206 702 677 1148 245 2120
Fragments arranged by size:
2120 1148 702 677 523 453 374 329
321 245 228 206 177 169 15 12
NaeI GCC'GGC
Cuts at: 2104 2518 4237 4924 2104
Size: 414 1719 687 4879
Fragments arranged by size:
4879 1719 687 414
NarI GG'CG_CC
Cuts at: 2595 2595
Size: 7699
NcoI C'CATG_G
Cuts at: 513 1065 2004 3145 4584 513
Size: 552 939 1141 1439 3628
Fragments arranged by size:
3628 1439 1141 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7699
NgoMIV G'CCGG_C
Cuts at: 2102 2516 4235 4922 2102
Size: 414 1719 687 4879
Fragments arranged by size:
4879 1719 687 414
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7699
NotI GC'GGCC_GC
Cuts at: 5146 5146
Size: 7699
NspI r_CATG'y
Cuts at: 1132 1229 2605 4219 4741 4963 5057 6602 1132
Size: 97 1376 1614 522 222 94 1545 2229
Fragments arranged by size:
2229 1614 1545 1376 522 222 97 94
PasI CC'CwG_GG
Cuts at: 1348 2870 4390 1348
Size: 1522 1520 4657
Fragments arranged by size:
4657 1522 1520
PciI A'CATG_T
Cuts at: 6598 6598
Size: 7699
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2183 3250 4210 4413 1108
Size: 1075 1067 960 203 4394
Fragments arranged by size:
4394 1075 1067 960 203
PfoI T'CCnGG_A
Cuts at: 2583 3453 3663 3811 4350 4752 4953 2583
Size: 870 210 148 539 402 201 5329
Fragments arranged by size:
5329 870 539 402 210 201 148
PmeI GTTT'AAAC
Cuts at: 5003 5003
Size: 7699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2318 2350 5768 5800 7281 7313 2318
Size: 32 3418 32 1481 32 2704
Fragments arranged by size:
3418 2704 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3094 3850 3094
Size: 756 6943
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7699
PsiI TTA'TAA
Cuts at: 5269 5269
Size: 7699
PspOMI G'GGCC_C
Cuts at: 2900 3651 2900
Size: 751 6948
PspXI vC'TCGA_Gb
Cuts at: 3074 4094 3074
Size: 1020 6679
PstI C_TGCA'G
Cuts at: 830 3798 4971 5051 830
Size: 2968 1173 80 3478
Fragments arranged by size:
3478 2968 1173 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7699
PvuII CAG'CTG
Cuts at: 2837 3211 3532 3701 4207 2837
Size: 374 321 169 506 6329
Fragments arranged by size:
6329 506 374 321 169
SacI G_AGCT'C
Cuts at: 2331 2331
Size: 7699
SalI G'TCGA_C
Cuts at: 1936 3954 5041 1936
Size: 2018 1087 4594
Fragments arranged by size:
4594 2018 1087
SapI GCTCTTCn'nnn_
Cuts at: 3476 3476
Size: 7699
SbfI CC_TGCA'GG
Cuts at: 5051 5051
Size: 7699
ScaI AGT'ACT
Cuts at: 3473 5947 7659 3473
Size: 2474 1712 3513
Fragments arranged by size:
3513 2474 1712
SexAI A'CCwGG_T
Cuts at: 2238 4311 4869 2238
Size: 2073 558 5068
Fragments arranged by size:
5068 2073 558
SfcI C'TryA_G
Cuts at: 826 1045 3696 3794 4967 5047 6182 6863 7054
Size: 219 2651 98 1173 80 1135 681 191
Cuts at: 7054 826
Size: 1471
Fragments arranged by size:
2651 1471 1173 1135 681 219 191 98
80
SfoI GGC'GCC
Cuts at: 2596 2596
Size: 7699
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7699
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7699
SmaI CCC'GGG
Cuts at: 1872 3092 5026 1872
Size: 1220 1934 4545
Fragments arranged by size:
4545 1934 1220
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2330 2759 3074 4094 4709 5803
Size: 197 926 387 429 315 1020 615 1094
Cuts at: 5803 6704 6966 820
Size: 901 262 1553
Fragments arranged by size:
1553 1094 1020 926 901 615 429 387
315 262 197
SnaBI TAC'GTA
Cuts at: 493 5490 493
Size: 4997 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7699
SphI G_CATG'C
Cuts at: 1132 5057 1132
Size: 3925 3774
SspI AAT'ATT
Cuts at: 5 52 5623 5
Size: 47 5571 2081
Fragments arranged by size:
5571 2081 47
StuI AGG'CCT
Cuts at: 1517 4052 1517
Size: 2535 5164
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 2559 2841 3145 3879 4584
Size: 552 939 528 27 282 304 734 705
Cuts at: 4584 5138 513
Size: 554 3074
Fragments arranged by size:
3074 939 734 705 554 552 528 304
282 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2175 2792 3089 4597 4618 5726 5743
Size: 1255 595 617 297 1508 21 1108 17
Cuts at: 5743 5896 6081 6526 325
Size: 153 185 445 1498
Fragments arranged by size:
1508 1498 1255 1108 617 595 445 297
185 153 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3120 3471 3603
Size: 274 80 33 51 1443 1143 351 132
Cuts at: 3603 3780 3801 5945 7657 96
Size: 177 21 2144 1712 138
Fragments arranged by size:
2144 1712 1443 1143 351 274 177 138
132 80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2837 2869 4734 4766 7659 7691 2837
Size: 32 1865 32 2893 32 2845
Fragments arranged by size:
2893 2845 1865 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7699
XbaI T'CTAG_A
Cuts at: 5035 5035
Size: 7699
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2184 2184
Size: 7699
XhoI C'TCGA_G
Cuts at: 1943 3074 4094 1943
Size: 1131 1020 5548
Fragments arranged by size:
5548 1131 1020
XmaI C'CCGG_G
Cuts at: 1870 3090 5024 1870
Size: 1220 1934 4545
Fragments arranged by size:
4545 1934 1220
XmnI GAAnn'nnTTC
Cuts at: 5828 5828
Size: 7699
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5507 276
Size: 53 83 186 936 3973 2468
Fragments arranged by size:
3973 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV FspAI
NruI NsiI PacI PmlI PsrI RsrII SacII SanDI
SfiI SrfI SwaI