Restriction map for
pFN21AB7017
(Circular) (Six-base) MAPSORT of: pFN21AB7017.seq Check: 4716 from: 1 to: 7369
pFN21AB7017 7369 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2855 3410 5179 278
Size: 53 83 186 936 1319 555 1769 2468
Fragments arranged by size:
2468 1769 1319 936 555 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 4712 1937
Size: 790 1985 4594
Fragments arranged by size:
4594 1985 790
Acc65I G'GTAC_C
Cuts at: 2301 4690 2301
Size: 2389 4980
AclI AA'CG_TT
Cuts at: 1563 5496 5869 1563
Size: 3933 373 3063
Fragments arranged by size:
3933 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2882 3835 5432 6816 7084 1632
Size: 1250 953 1597 1384 268 1917
Fragments arranged by size:
1917 1597 1384 1250 953 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7172
AflIII A'CryG_T
Cuts at: 1203 2346 3702 4545 6268 7245 1203
Size: 1143 1356 843 1723 977 1327
Fragments arranged by size:
1723 1356 1327 1143 977 843
AgeI A'CCGG_T
Cuts at: 6200 6200
Size: 7369
AhdI GACnn_n'nnGTC
Cuts at: 6098 6098
Size: 7369
AleI CACnn'nnGTG
Cuts at: 1166 2933 1166
Size: 1767 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3177 1184
Size: 1993 5376
AlwNI CAG_nnn'CTG
Cuts at: 2588 3496 5112 6684 2588
Size: 908 1616 1572 3273
Fragments arranged by size:
3273 1616 1572 908
ApaI G_GGCC'C
Cuts at: 2163 3646 4101 4199 4279 4424 4508 2163
Size: 1483 455 98 80 145 84 5024
Fragments arranged by size:
5024 1483 455 145 98 84 80
ApaLI G'TGCA_C
Cuts at: 5426 6582 5426
Size: 1156 6213
ApoI r'AATT_y
Cuts at: 1493 4678 4904 5056 7165 1493
Size: 3185 226 152 2109 1697
Fragments arranged by size:
3185 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5923 160
Size: 872 4891 1606
Fragments arranged by size:
4891 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7369
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 2796 2945 3077 3128 3146 3288
Size: 73 690 163 149 132 51 18 142
Cuts at: 3288 3592 3673 3989 4114 4133 4272 4280 4694
Size: 304 81 316 125 19 139 8 414
Cuts at: 4694 1870
Size: 4545
Fragments arranged by size:
4545 690 414 316 304 163 149 142
139 132 125 81 73 51 19 18
8
BamHI G'GATC_C
Cuts at: 3255 4699 5066 3255
Size: 1444 367 5558
Fragments arranged by size:
5558 1444 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2301 3097 3112 3229
Size: 325 217 627 282 232 796 15 117
Cuts at: 3229 3276 3677 4019 4048 4264 4690 6145 618
Size: 47 401 342 29 216 426 1455 1842
Fragments arranged by size:
1842 1455 796 627 426 401 342 325
282 232 217 216 117 47 29 15
BanII G_rGCy'C
Cuts at: 1391 2163 2280 3417 3646 4101 4199 4279 4424
Size: 772 117 1137 229 455 98 80 145
Cuts at: 4424 4472 4508 4688 1391
Size: 48 36 180 4072
Fragments arranged by size:
4072 1137 772 455 229 180 145 117
98 80 48 36
BbeI G_GCGC'C
Cuts at: 2073 4023 4052 4268 2073
Size: 1950 29 216 5174
Fragments arranged by size:
5174 1950 216 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3331 3415 928
Size: 971 1432 84 4882
Fragments arranged by size:
4882 1432 971 84
BbvCI CC'TCA_GC
Cuts at: 3668 4438 3668
Size: 770 6599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5560 5594 1903
Size: 34 50 34 3539 34 3678
Fragments arranged by size:
3678 3539 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5261 6471 5261
Size: 1210 6159
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7369
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2949 3321 3923
Size: 107 122 71 631 605 1277 372 602
Cuts at: 3923 3966 4046 4520 4587 5980 136
Size: 43 80 474 67 1393 1525
Fragments arranged by size:
1525 1393 1277 631 605 602 474 372
122 107 80 71 67 43
BglII A'GATC_T
Cuts at: 2324 7364 2324
Size: 5040 2329
BlpI GC'TnA_GC
Cuts at: 2105 3500 3950 4786 2105
Size: 1395 450 836 4688
Fragments arranged by size:
4688 1395 836 450
Bme1580I G_kGCm'C
Cuts at: 1790 2163 3234 3646 3680 4101 4199 4279 4424
Size: 373 1071 412 34 421 98 80 145
Cuts at: 4424 4508 4610 4668 5430 6586 1790
Size: 84 102 58 762 1156 2573
Fragments arranged by size:
2573 1156 1071 762 421 412 373 145
102 98 84 80 58 34
BmgBI CAC'GTC
Cuts at: 1318 2286 2815 3304 3703 1318
Size: 968 529 489 399 4984
Fragments arranged by size:
4984 968 529 489 399
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 4511 5100
Size: 416 53 83 399 24 655 2439 589
Cuts at: 5100 6058 442
Size: 958 1753
Fragments arranged by size:
2439 1753 958 655 589 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2341 2789 2858 3151 3449 4486 6029
Size: 1055 47 448 69 293 298 1037 1543
Cuts at: 6029 1239
Size: 2579
Fragments arranged by size:
2579 1543 1055 1037 448 298 293 69
47
Bpu10I CC'TnA_GC
Cuts at: 3668 3727 4438 3668
Size: 59 711 6599
Fragments arranged by size:
6599 711 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3353 5494 6359 6657 3353
Size: 2141 865 298 4065
Fragments arranged by size:
4065 2141 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3434 6032 882
Size: 617 1935 2598 2219
Fragments arranged by size:
2598 2219 1935 617
BsaAI yAC'GTr
Cuts at: 493 1204 5160 493
Size: 711 3956 2702
Fragments arranged by size:
3956 2702 711
BsaBI GATnn'nnATC
Cuts at: 7363 7363
Size: 7369
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2167 2852 3092
Size: 53 83 186 936 537 97 685 240
Cuts at: 3092 3407 4020 4049 4142 4265 5176 5558 6193
Size: 315 613 29 93 123 911 382 635
Cuts at: 6193 275
Size: 1451
Fragments arranged by size:
1451 936 911 685 635 613 537 382
315 240 186 123 97 93 83 53
29
BsaWI w'CCGG_w
Cuts at: 5802 6200 6474 6621 7032 5802
Size: 398 274 147 411 6139
Fragments arranged by size:
6139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 2363 2393 2831
Size: 30 165 30 882 30 48 30 438
Cuts at: 2831 2861 3149 3179 3825 3855 1178
Size: 30 288 30 646 30 4692
Fragments arranged by size:
4692 882 646 438 288 165 48 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 3446 4507 4616 1186
Size: 919 1341 1061 109 3939
Fragments arranged by size:
3939 1341 1061 919 109
BseYI C'CCAG_C
Cuts at: 2333 2984 3238 4068 4432 6572 7068 2333
Size: 651 254 830 364 2140 496 2634
Fragments arranged by size:
2634 2140 830 651 496 364 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3721 1163
Size: 573 1985 4811
Fragments arranged by size:
4811 1985 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3452 4321 4345 4524 4604 4819
Size: 275 186 1451 869 24 179 80 215
Cuts at: 4819 5580 6608 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1451 1028 869 761 275 215 186
179 80 24
BsiHKAI G_wGCw'C
Cuts at: 5430 5515 6586 5430
Size: 85 1071 6213
Fragments arranged by size:
6213 1071 85
BsiWI C'GTAC_G
Cuts at: 2529 2529
Size: 7369
BsmI GAATG_Cn'
Cuts at: 4880 4973 4880
Size: 93 7276
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2280 3234 3417 3646 3680 4101
Size: 399 373 117 954 183 229 34 421
Cuts at: 4101 4199 4279 4424 4472 4508 4610 4668 4688
Size: 98 80 145 48 36 102 58 20
Cuts at: 4688 5430 5515 6586 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 954 742 421 399 373 229
183 145 117 102 98 85 80 58
48 36 34 20
BspEI T'CCGG_A
Cuts at: 7032 7032
Size: 7369
BspHI T'CATG_A
Cuts at: 5258 6988 5258
Size: 1730 5639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3804 4724 844
Size: 2960 920 3489
Fragments arranged by size:
3489 2960 920
BsrBI CCG'CTC
Cuts at: 1394 1823 3883 4369 5256 1394
Size: 429 2060 486 887 3507
Fragments arranged by size:
3507 2060 887 486 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5864 6038 65
Size: 1183 4616 174 1396
Fragments arranged by size:
4616 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2952 3094 3311 3447 4395 6013 6200
Size: 110 1142 142 217 136 948 1618 187
Cuts at: 6200 1700
Size: 2869
Fragments arranged by size:
2869 1618 1142 948 217 187 142 136
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7369
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7369
BssSI C'ACGA_G
Cuts at: 5429 6441 5429
Size: 1012 6357
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2186 3496 794
Size: 1392 1310 4667
Fragments arranged by size:
4667 1392 1310
BstBI TT'CG_AA
Cuts at: 6173 6173
Size: 7369
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7369
BstYI r'GATC_y
Cuts at: 1972 2324 3255 4699 5066 5451 5468 6920 7006
Size: 352 931 1444 367 385 17 1452 86
Cuts at: 7006 7018 7364 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1444 931 385 367 352 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2088 3400 3721 4490 2088
Size: 1312 321 769 4967
Fragments arranged by size:
4967 1312 769 321
BtgI C'CryG_G
Cuts at: 513 1065 2074 2289 2958 3837 4213 4354 4416
Size: 552 1009 215 669 879 376 141 62
Cuts at: 4416 4457 4585 513
Size: 41 128 3297
Fragments arranged by size:
3297 1009 879 669 552 376 215 141
128 62 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2765 487
Size: 683 56 1539 5091
Fragments arranged by size:
5091 1539 683 56
BtsI GCAGTG_nn'
Cuts at: 2852 4885 5679 5699 2852
Size: 2033 794 20 4522
Fragments arranged by size:
4522 2033 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7369
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2410 2445 529
Size: 35 1846 35 5453
Fragments arranged by size:
5453 1846 35 35
DraI TTT'AAA
Cuts at: 4673 4673
Size: 7369
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6376 809
Size: 507 5060 1802
Fragments arranged by size:
5060 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2967 3449 4216
Size: 54 1425 50 275 18 1137 482 767
Cuts at: 4216 4318 4342 4521 4601 4816 5705 7334 8
Size: 102 24 179 80 215 889 1629 43
Fragments arranged by size:
1629 1425 1137 889 767 482 275 215
179 102 80 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3449 4318 4342 4521 4601 4816 1537
Size: 275 1637 869 24 179 80 215 4090
Fragments arranged by size:
4090 1637 869 275 215 179 80 24
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 2582 5299 1353
Size: 1211 18 2717 3423
Fragments arranged by size:
3423 2717 1211 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4017 4658 5956 6330 6476 387
Size: 1064 138 2428 641 1298 374 146 1280
Fragments arranged by size:
2428 1298 1280 1064 641 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2341 2789 2858 2882 3151 3449
Size: 393 662 47 448 69 24 269 298
Cuts at: 3449 3835 4486 5432 6029 6816 7084 1239
Size: 386 651 946 597 787 268 1524
Fragments arranged by size:
1524 946 787 662 651 597 448 393
386 298 269 268 69 47 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2771 3029 3764 2771
Size: 258 735 6376
Fragments arranged by size:
6376 735 258
EcoO109I rG'GnC_Cy
Cuts at: 3529 3625 3643 3986 4039 4098 4196 4276 4421
Size: 96 18 343 53 59 98 80 145
Cuts at: 4421 4505 3529
Size: 84 6393
Fragments arranged by size:
6393 343 145 98 96 84 80 59
53 18
EcoRI G'AATT_C
Cuts at: 4678 5056 4678
Size: 378 6991
FseI GG_CCGG'CC
Cuts at: 2956 2956
Size: 7369
FspI TGC'GCA
Cuts at: 2654 3139 5875 2654
Size: 485 2736 4148
Fragments arranged by size:
4148 2736 485
HaeII r_GCGC'y
Cuts at: 1678 2073 3174 4023 4052 4162 4268 6516 1678
Size: 395 1101 849 29 110 106 2248 2531
Fragments arranged by size:
2531 2248 1101 849 395 110 106 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2490 2522 3056 3088 3196
Size: 32 135 32 918 32 534 32 108
Cuts at: 3196 3228 4097 4129 4421 4453 6010 6042 6084
Size: 32 869 32 292 32 1557 32 42
Cuts at: 6084 6116 1373
Size: 32 2626
Fragments arranged by size:
2626 1557 918 869 534 292 135 108
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 4713 4959 669
Size: 988 281 286 2489 246 3079
Fragments arranged by size:
3079 2489 988 286 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7369
HpaI GTT'AAC
Cuts at: 4959 4959
Size: 7369
KasI G'GCGC_C
Cuts at: 2069 4019 4048 4264 2069
Size: 1950 29 216 5174
Fragments arranged by size:
5174 1950 216 29
KpnI G_GTAC'C
Cuts at: 2305 4694 2305
Size: 2389 4980
MfeI C'AATT_G
Cuts at: 4968 4968
Size: 7369
MluI A'CGCG_T
Cuts at: 7245 7245
Size: 7369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2333 2466 2476 6483 6667 1305
Size: 1028 133 10 4007 184 2007
Fragments arranged by size:
4007 2007 1028 184 133 10
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5547
Fragments arranged by size:
5547 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 2547 2933 5327 5686 5845 518
Size: 648 1179 202 386 2394 359 159 2042
Fragments arranged by size:
2394 2042 1179 648 386 359 202 159
MspA1I CmG'CkG
Cuts at: 1606 2157 2511 2523 3041 3283 3493 3552 4147
Size: 551 354 12 518 242 210 59 595
Cuts at: 4147 4459 4557 4587 4785 5462 6610 6855 1606
Size: 312 98 30 198 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 595 551 518 354 312
245 242 210 198 98 59 30 12
NaeI GCC'GGC
Cuts at: 2954 3313 4397 2954
Size: 359 1084 5926
Fragments arranged by size:
5926 1084 359
NarI GG'CG_CC
Cuts at: 2070 4020 4049 4265 2070
Size: 1950 29 216 5174
Fragments arranged by size:
5174 1950 216 29
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6817
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7369
NgoMIV G'CCGG_C
Cuts at: 2952 3311 4395 2952
Size: 359 1084 5926
Fragments arranged by size:
5926 1084 359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7369
NotI GC'GGCC_GC
Cuts at: 4816 4816
Size: 7369
NspI r_CATG'y
Cuts at: 1132 1229 4727 6272 1132
Size: 97 3498 1545 2229
Fragments arranged by size:
3498 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2118 3318 4192 1348
Size: 770 1200 874 4525
Fragments arranged by size:
4525 1200 874 770
PciI A'CATG_T
Cuts at: 6268 6268
Size: 7369
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2707 4108 4192 1108
Size: 1599 1401 84 4285
Fragments arranged by size:
4285 1599 1401 84
PfoI T'CCnGG_A
Cuts at: 2739 3426 2739
Size: 687 6682
PmeI GTTT'AAAC
Cuts at: 4673 4673
Size: 7369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5438 5470 6951 6983 5438
Size: 32 1481 32 5824
Fragments arranged by size:
5824 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3625 3986 3625
Size: 361 7008
PshAI GACnn'nnGTC
Cuts at: 1613 3335 1613
Size: 1722 5647
PsiI TTA'TAA
Cuts at: 4939 4939
Size: 7369
PspOMI G'GGCC_C
Cuts at: 2159 3642 4097 4195 4275 4420 4504 2159
Size: 1483 455 98 80 145 84 5024
Fragments arranged by size:
5024 1483 455 145 98 84 80
PspXI vC'TCGA_Gb
Cuts at: 3288 3288
Size: 7369
PstI C_TGCA'G
Cuts at: 830 2490 2922 4092 4721 830
Size: 1660 432 1170 629 3478
Fragments arranged by size:
3478 1660 1170 629 432
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7369
PvuII CAG'CTG
Cuts at: 3041 3283 3041
Size: 242 7127
RsrII CG'GwC_CG
Cuts at: 4390 4390
Size: 7369
SacII CC_GC'GG
Cuts at: 4460 4588 4460
Size: 128 7241
SalI G'TCGA_C
Cuts at: 1936 4711 1936
Size: 2775 4594
SanDI GG'GwC_CC
Cuts at: 3986 3986
Size: 7369
SapI GCTCTTCn'nnn_
Cuts at: 2582 2582
Size: 7369
SbfI CC_TGCA'GG
Cuts at: 4721 4721
Size: 7369
ScaI AGT'ACT
Cuts at: 3590 5617 7329 3590
Size: 2027 1712 3630
Fragments arranged by size:
3630 2027 1712
SfcI C'TryA_G
Cuts at: 826 1045 2470 2486 2918 4088 4717 5852 6533
Size: 219 1425 16 432 1170 629 1135 681
Cuts at: 6533 6724 826
Size: 191 1471
Fragments arranged by size:
1471 1425 1170 1135 681 629 432 219
191 16
SfoI GGC'GCC
Cuts at: 2071 4021 4050 4266 2071
Size: 1950 29 216 5174
Fragments arranged by size:
5174 1950 216 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7369
SgrAI Cr'CCGG_yG
Cuts at: 1700 3094 4395 1700
Size: 1394 1301 4674
Fragments arranged by size:
4674 1394 1301
SmaI CCC'GGG
Cuts at: 1872 3130 3148 3675 3991 4274 4696 1872
Size: 1258 18 527 316 283 422 4545
Fragments arranged by size:
4545 1258 527 422 316 283 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3288 3368 5473 6374 6636 820
Size: 197 926 1345 80 2105 901 262 1553
Fragments arranged by size:
2105 1553 1345 926 901 262 197 80
SnaBI TAC'GTA
Cuts at: 493 5160 493
Size: 4667 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7369
SphI G_CATG'C
Cuts at: 1132 4727 1132
Size: 3595 3774
SrfI GCCC'GGGC
Cuts at: 3675 4274 3675
Size: 599 6770
SspI AAT'ATT
Cuts at: 5 52 5293 5
Size: 47 5241 2081
Fragments arranged by size:
5241 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7369
StyI C'CwwG_G
Cuts at: 513 1065 4074 4425 4808 513
Size: 552 3009 351 383 3074
Fragments arranged by size:
3074 3009 552 383 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4556 5396 5413 5566 5751 6196 325
Size: 1255 2976 840 17 153 185 445 1498
Fragments arranged by size:
2976 1498 1255 840 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3156 3588 5615
Size: 274 80 33 51 1443 1179 432 2027
Cuts at: 5615 7327 96
Size: 1712 138
Fragments arranged by size:
2027 1712 1443 1179 432 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 2831 2863 3825 3857 7329 7361 2363
Size: 32 436 32 962 32 3472 32 2371
Fragments arranged by size:
3472 2371 962 436 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7369
XbaI T'CTAG_A
Cuts at: 4705 4705
Size: 7369
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4423 4423
Size: 7369
XhoI C'TCGA_G
Cuts at: 1943 3288 1943
Size: 1345 6024
XmaI C'CCGG_G
Cuts at: 1870 3128 3146 3673 3989 4272 4694 1870
Size: 1258 18 527 316 283 422 4545
Fragments arranged by size:
4545 1258 527 422 316 283 18
XmnI GAAnn'nnTTC
Cuts at: 5498 5498
Size: 7369
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2853 3408 5177 276
Size: 53 83 186 936 1319 555 1769 2468
Fragments arranged by size:
2468 1769 1319 936 555 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FspAI
NruI NsiI PacI PmlI PsrI SacI SexAI SfiI
SwaI