Restriction map for
pFN21AB7016
(Circular) (Six-base) MAPSORT of: pFN21AB7016.seq Check: 2941 from: 1 to: 7210
pFN21AB7016 7210 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2696 3969 2696
Size: 1273 5937
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5020 278
Size: 53 83 186 936 3484 2468
Fragments arranged by size:
3484 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4553 1937
Size: 2616 4594
Acc65I G'GTAC_C
Cuts at: 3833 4531 3833
Size: 698 6512
AclI AA'CG_TT
Cuts at: 1563 5337 5710 1563
Size: 3774 373 3063
Fragments arranged by size:
3774 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4049 5273 6657 6925 1632
Size: 2417 1224 1384 268 1917
Fragments arranged by size:
2417 1917 1384 1224 268
AfeI AGC'GCT
Cuts at: 2099 4373 2099
Size: 2274 4936
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7013
AflIII A'CryG_T
Cuts at: 1203 6109 7086 1203
Size: 4906 977 1327
Fragments arranged by size:
4906 1327 977
AgeI A'CCGG_T
Cuts at: 6041 6041
Size: 7210
AhdI GACnn_n'nnGTC
Cuts at: 4351 5939 4351
Size: 1588 5622
AleI CACnn'nnGTG
Cuts at: 1166 2345 4076 1166
Size: 1179 1731 4300
Fragments arranged by size:
4300 1731 1179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7210
AlwNI CAG_nnn'CTG
Cuts at: 2499 3263 3611 4483 4953 6525 2499
Size: 764 348 872 470 1572 3184
Fragments arranged by size:
3184 1572 872 764 470 348
ApaI G_GGCC'C
Cuts at: 2069 2286 3130 3297 3719 2069
Size: 217 844 167 422 5560
Fragments arranged by size:
5560 844 422 217 167
ApaLI G'TGCA_C
Cuts at: 5267 6423 5267
Size: 1156 6054
ApoI r'AATT_y
Cuts at: 1493 4519 4745 4897 7006 1493
Size: 3026 226 152 2109 1697
Fragments arranged by size:
3026 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5764 160
Size: 872 4732 1606
Fragments arranged by size:
4732 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7210
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2068 2225 2311 2604 3856 4535
Size: 73 119 6 157 86 293 1252 679
Cuts at: 4535 1870
Size: 4545
Fragments arranged by size:
4545 1252 679 293 157 119 86 73
6
BamHI G'GATC_C
Cuts at: 3145 4540 4907 3145
Size: 1395 367 5448
Fragments arranged by size:
5448 1395 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 2339 3457 3632 3833
Size: 325 217 627 381 171 1118 175 201
Cuts at: 3833 4211 4531 5986 618
Size: 378 320 1455 1842
Fragments arranged by size:
1842 1455 1118 627 381 378 325 320
217 201 175 171
BanII G_rGCy'C
Cuts at: 1391 2069 2081 2238 2286 3061 3130 3289 3297
Size: 678 12 157 48 775 69 159 8
Cuts at: 3297 3314 3393 3697 3719 4440 4529 1391
Size: 17 79 304 22 721 89 4072
Fragments arranged by size:
4072 775 721 678 304 159 157 89
79 69 48 22 17 12 8
BbeI G_GCGC'C
Cuts at: 2172 2172
Size: 7210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3511 3520 928
Size: 971 1612 9 4618
Fragments arranged by size:
4618 1612 971 9
BbvCI CC'TCA_GC
Cuts at: 2137 3765 3838 4049 2137
Size: 1628 73 211 5298
Fragments arranged by size:
5298 1628 211 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2025 2059 4435 4469 5401 5435 1903
Size: 34 88 34 2376 34 932 34 3678
Fragments arranged by size:
3678 2376 932 88 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5102 6312 5102
Size: 1210 6000
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7210
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2018 2114
Size: 107 122 71 631 605 337 9 96
Cuts at: 2114 3056 5821 136
Size: 942 2765 1525
Fragments arranged by size:
2765 1525 942 631 605 337 122 107
96 71 9
BglII A'GATC_T
Cuts at: 7205 7205
Size: 7210
BlpI GC'TnA_GC
Cuts at: 4627 4627
Size: 7210
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2286 3130 3297 3637 3719 4240 5271
Size: 279 217 844 167 340 82 521 1031
Cuts at: 5271 6427 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1031 844 521 340 279 217
167 82
BmgBI CAC'GTC
Cuts at: 1318 4348 1318
Size: 3030 4180
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2598 4941 5899
Size: 416 53 83 399 24 1181 2343 958
Cuts at: 5899 442
Size: 1753
Fragments arranged by size:
2343 1753 1181 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7210
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3853 3853
Size: 7210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2210 3008 3083 3833 4229 5870 1239
Size: 962 9 798 75 750 396 1641 2579
Fragments arranged by size:
2579 1641 962 798 750 396 75 9
Bpu10I CC'TnA_GC
Cuts at: 2137 2278 3299 3421 3765 3805 3838 4049 2137
Size: 141 1021 122 344 40 33 211 5298
Fragments arranged by size:
5298 1021 344 211 141 122 40 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3364 5335 6200 6498 3116
Size: 248 1971 865 298 3828
Fragments arranged by size:
3828 1971 865 298 248
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 3716 5873 882
Size: 617 1023 1194 2157 2219
Fragments arranged by size:
2219 2157 1194 1023 617
BsaAI yAC'GTr
Cuts at: 493 1204 5001 493
Size: 711 3797 2702
Fragments arranged by size:
3797 2702 711
BsaBI GATnn'nnATC
Cuts at: 2897 7204 2897
Size: 4307 2903
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2169 2439 5017
Size: 53 83 186 936 614 22 270 2578
Cuts at: 5017 5399 6034 275
Size: 382 635 1451
Fragments arranged by size:
2578 1451 936 635 614 382 270 186
83 53 22
BsaWI w'CCGG_w
Cuts at: 5643 6041 6315 6462 6873 5643
Size: 398 274 147 411 5980
Fragments arranged by size:
5980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 4241 4271 1178
Size: 30 165 30 1104 30 1704 30 4117
Fragments arranged by size:
4117 1704 1104 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3683 3686 4004 4049 1186
Size: 2497 3 318 45 4347
Fragments arranged by size:
4347 2497 318 45 3
BseYI C'CCAG_C
Cuts at: 2485 6413 6909 2485
Size: 3928 496 2786
Fragments arranged by size:
3928 2786 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2227 2446 3104 3249 3359 3405 4083
Size: 573 491 219 658 145 110 46 678
Cuts at: 4083 4370 1163
Size: 287 4003
Fragments arranged by size:
4003 678 658 573 491 287 219 145
110 46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2118 4660 5421 6449 1540
Size: 275 186 48 69 2542 761 1028 2301
Fragments arranged by size:
2542 2301 1028 761 275 186 69 48
BsiHKAI G_wGCw'C
Cuts at: 2196 2513 2825 3036 4020 5271 5356 6427 2196
Size: 317 312 211 984 1251 85 1071 2979
Fragments arranged by size:
2979 1251 1071 984 317 312 211 85
BsmI GAATG_Cn'
Cuts at: 4721 4814 4721
Size: 93 7117
BsmBI CGTCTCn'nnnn_
Cuts at: 3668 4376 3668
Size: 708 6502
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2081 2196 2238 2286 2513 2825
Size: 399 279 12 115 42 48 227 312
Cuts at: 2825 3036 3061 3130 3289 3297 3314 3393 3637
Size: 211 25 69 159 8 17 79 244
Cuts at: 3637 3697 3719 4020 4240 4440 4529 5271 5356
Size: 60 22 301 220 200 89 742 85
Cuts at: 5356 6427 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 312 301 279 244
227 220 211 200 159 115 89 85
79 69 60 48 42 25 22 17
12 8
BspEI T'CCGG_A
Cuts at: 6873 6873
Size: 7210
BspHI T'CATG_A
Cuts at: 2910 5099 6829 2910
Size: 2189 1730 3291
Fragments arranged by size:
3291 2189 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2696 3969 4565 844
Size: 1852 1273 596 3489
Fragments arranged by size:
3489 1852 1273 596
BsrBI CCG'CTC
Cuts at: 1394 1823 2992 3420 3441 5097 1394
Size: 429 1169 428 21 1656 3507
Fragments arranged by size:
3507 1656 1169 429 428 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 2810 5705 5879 65
Size: 1183 1562 2895 174 1396
Fragments arranged by size:
2895 1562 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3596 3964 5854 6041 1700
Size: 110 1786 368 1890 187 2869
Fragments arranged by size:
2869 1890 1786 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7210
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7210
BssSI C'ACGA_G
Cuts at: 3462 5270 6282 3462
Size: 1808 1012 4390
Fragments arranged by size:
4390 1808 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3040 794
Size: 2246 4964
BstBI TT'CG_AA
Cuts at: 6014 6014
Size: 7210
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7210
BstXI CCAn_nnnn'nTGG
Cuts at: 4208 4208
Size: 7210
BstYI r'GATC_y
Cuts at: 1972 3015 3145 4219 4540 4907 5292 5309 6761
Size: 1043 130 1074 321 367 385 17 1452
Cuts at: 6761 6847 6859 7205 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1074 1043 385 367 346 321
130 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3828 3828
Size: 7210
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2142 2157 2286 2343 3150
Size: 552 939 12 126 15 129 57 807
Cuts at: 3150 3270 3330 513
Size: 120 60 4393
Fragments arranged by size:
4393 939 807 552 129 126 120 60
57 15 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6471
Fragments arranged by size:
6471 683 56
BtsI GCAGTG_nn'
Cuts at: 2194 2754 4726 5520 5540 2194
Size: 560 1972 794 20 3864
Fragments arranged by size:
3864 1972 794 560 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7210
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7175
DraI TTT'AAA
Cuts at: 4514 4514
Size: 7210
DraIII CAC_nnn'GTG
Cuts at: 3791 3791
Size: 7210
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6217 809
Size: 507 4901 1802
Fragments arranged by size:
4901 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2115 2895
Size: 54 1425 50 275 18 216 69 780
Cuts at: 2895 3598 3654 4083 4657 5546 7175 8
Size: 703 56 429 574 889 1629 43
Fragments arranged by size:
1629 1425 889 780 703 574 429 275
216 69 56 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 2115 4657 1537
Size: 275 234 69 2542 4090
Fragments arranged by size:
4090 2542 275 234 69
EarI CTCTTCn'nnn_
Cuts at: 1353 2813 5140 1353
Size: 1460 2327 3423
Fragments arranged by size:
3423 2327 1460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2837 5797 6171 6317 387
Size: 1064 138 1248 2960 374 146 1280
Fragments arranged by size:
2960 1280 1248 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2210 3008 3083 3833 4049 4229
Size: 393 569 9 798 75 750 216 180
Cuts at: 4229 5273 5870 6657 6925 1239
Size: 1044 597 787 268 1524
Fragments arranged by size:
1524 1044 798 787 750 597 569 393
268 216 180 75 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4031 4031
Size: 7210
EcoO109I rG'GnC_Cy
Cuts at: 2282 3127 3184 3260 3293 3294 3715 3716 3755
Size: 845 57 76 33 1 421 1 39
Cuts at: 3755 3770 2282
Size: 15 5722
Fragments arranged by size:
5722 845 421 76 57 39 33 15
1 1
EcoRI G'AATT_C
Cuts at: 4519 4897 4519
Size: 378 6832
FspI TGC'GCA
Cuts at: 3452 5716 3452
Size: 2264 4946
HaeII r_GCGC'y
Cuts at: 1678 2101 2172 2180 2218 2791 2986 3582 4375
Size: 423 71 8 38 573 195 596 793
Cuts at: 4375 6357 1678
Size: 1982 2531
Fragments arranged by size:
2531 1982 793 596 573 423 195 71
38 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3130 3162 3362 3394 4241
Size: 32 135 32 1558 32 200 32 847
Cuts at: 4241 4273 4337 4369 5851 5883 5925 5957 1373
Size: 32 64 32 1482 32 42 32 2626
Fragments arranged by size:
2626 1558 1482 847 200 135 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4329 4554 4800 669
Size: 988 281 2391 225 246 3079
Fragments arranged by size:
3079 2391 988 281 246 225
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7210
HpaI GTT'AAC
Cuts at: 4329 4800 4329
Size: 471 6739
KasI G'GCGC_C
Cuts at: 2168 2168
Size: 7210
KpnI G_GTAC'C
Cuts at: 3837 4535 3837
Size: 698 6512
MfeI C'AATT_G
Cuts at: 4809 4809
Size: 7210
MluI A'CGCG_T
Cuts at: 7086 7086
Size: 7210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6324 6508 1305
Size: 5019 184 2007
Fragments arranged by size:
5019 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2897 3656 4085 10
Size: 54 1425 343 1065 759 429 3135
Fragments arranged by size:
3135 1425 1065 759 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 2643 3335 4076 5168 5527 5686
Size: 648 1179 298 692 741 1092 359 159
Cuts at: 5686 518
Size: 2042
Fragments arranged by size:
2042 1179 1092 741 692 648 359 298
159
MspA1I CmG'CkG
Cuts at: 1606 2018 2039 2084 2144 2159 2288 2551 3118
Size: 412 21 45 60 15 129 263 567
Cuts at: 3118 3500 3587 4108 4171 4626 5303 6451 6696
Size: 382 87 521 63 455 677 1148 245
Cuts at: 6696 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 567 521 455 412 382
263 245 129 87 63 60 45 21
15
NaeI GCC'GGC
Cuts at: 3966 3966
Size: 7210
NarI GG'CG_CC
Cuts at: 2169 2169
Size: 7210
NcoI C'CATG_G
Cuts at: 513 1065 2004 3270 3330 513
Size: 552 939 1266 60 4393
Fragments arranged by size:
4393 1266 939 552 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7210
NgoMIV G'CCGG_C
Cuts at: 3964 3964
Size: 7210
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7210
NotI GC'GGCC_GC
Cuts at: 4657 4657
Size: 7210
NspI r_CATG'y
Cuts at: 1132 1229 2807 4309 4568 6113 1132
Size: 97 1578 1502 259 1545 2229
Fragments arranged by size:
2229 1578 1545 1502 259 97
PasI CC'CwG_GG
Cuts at: 1348 3290 1348
Size: 1942 5268
PciI A'CATG_T
Cuts at: 6109 6109
Size: 7210
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3331 3851 4297 1108
Size: 2223 520 446 4021
Fragments arranged by size:
4021 2223 520 446
PfoI T'CCnGG_A
Cuts at: 4245 4245
Size: 7210
PmeI GTTT'AAAC
Cuts at: 4514 4514
Size: 7210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5279 5311 6792 6824 5279
Size: 32 1481 32 5665
Fragments arranged by size:
5665 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3184 3755 3770 3184
Size: 571 15 6624
Fragments arranged by size:
6624 571 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7210
PsiI TTA'TAA
Cuts at: 4780 4780
Size: 7210
PspOMI G'GGCC_C
Cuts at: 2065 2282 3126 3293 3715 2065
Size: 217 844 167 422 5560
Fragments arranged by size:
5560 844 422 217 167
PstI C_TGCA'G
Cuts at: 830 2907 3592 3622 3975 4371 4562 830
Size: 2077 685 30 353 396 191 3478
Fragments arranged by size:
3478 2077 685 396 353 191 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7210
PvuII CAG'CTG
Cuts at: 3118 3500 4171 3118
Size: 382 671 6157
Fragments arranged by size:
6157 671 382
SacII CC_GC'GG
Cuts at: 2019 2145 2160 2289 2019
Size: 126 15 129 6940
Fragments arranged by size:
6940 129 126 15
SalI G'TCGA_C
Cuts at: 1936 4552 1936
Size: 2616 4594
SanDI GG'GwC_CC
Cuts at: 3770 3770
Size: 7210
SapI GCTCTTCn'nnn_
Cuts at: 2813 2813
Size: 7210
SbfI CC_TGCA'GG
Cuts at: 3622 3975 4562 3622
Size: 353 587 6270
Fragments arranged by size:
6270 587 353
ScaI AGT'ACT
Cuts at: 5458 7170 5458
Size: 1712 5498
SfcI C'TryA_G
Cuts at: 826 1045 2266 2903 3588 3618 3971 4267 4367
Size: 219 1221 637 685 30 353 296 100
Cuts at: 4367 4558 5693 6374 6565 826
Size: 191 1135 681 191 1471
Fragments arranged by size:
1471 1221 1135 685 681 637 353 296
219 191 191 100 30
SfoI GGC'GCC
Cuts at: 2170 2170
Size: 7210
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7210
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7210
SmaI CCC'GGG
Cuts at: 1872 2064 2070 3858 4537 1872
Size: 192 6 1788 679 4545
Fragments arranged by size:
4545 1788 679 192 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3131 3379 5314 6215 6477 820
Size: 197 926 1188 248 1935 901 262 1553
Fragments arranged by size:
1935 1553 1188 926 901 262 248 197
SnaBI TAC'GTA
Cuts at: 493 5001 493
Size: 4508 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7210
SphI G_CATG'C
Cuts at: 1132 4568 1132
Size: 3436 3774
SrfI GCCC'GGGC
Cuts at: 2064 2070 2064
Size: 6 7204
SspI AAT'ATT
Cuts at: 5 52 5134 5
Size: 47 5082 2081
Fragments arranged by size:
5082 2081 47
StuI AGG'CCT
Cuts at: 1517 3215 4400 1517
Size: 1698 1185 4327
Fragments arranged by size:
4327 1698 1185
StyI C'CwwG_G
Cuts at: 513 1065 2004 2565 2791 2826 3270 3330 4649
Size: 552 939 561 226 35 444 60 1319
Cuts at: 4649 513
Size: 3074
Fragments arranged by size:
3074 1319 939 561 552 444 226 60
35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3283 5237 5254 5407 5592 6037 325
Size: 1255 1703 1954 17 153 185 445 1498
Fragments arranged by size:
1954 1703 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2559 4123 5456
Size: 274 80 33 51 1443 582 1564 1333
Cuts at: 5456 7168 96
Size: 1712 138
Fragments arranged by size:
1712 1564 1443 1333 582 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2507 2539 3190 3222 7170 7202 2507
Size: 32 651 32 3948 32 2515
Fragments arranged by size:
3948 2515 651 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7210
XbaI T'CTAG_A
Cuts at: 4546 4546
Size: 7210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2500 3808 3990 4192 4484 2389
Size: 111 1308 182 202 292 5115
Fragments arranged by size:
5115 1308 292 202 182 111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7210
XmaI C'CCGG_G
Cuts at: 1870 2062 2068 3856 4535 1870
Size: 192 6 1788 679 4545
Fragments arranged by size:
4545 1788 679 192 6
XmnI GAAnn'nnTTC
Cuts at: 2402 5339 2402
Size: 2937 4273
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5018 276
Size: 53 83 186 936 3484 2468
Fragments arranged by size:
3484 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SexAI SfiI SwaI