Restriction map for
pFN21AB7015
(Circular) (Six-base) MAPSORT of: pFN21AB7015.seq Check: 1092 from: 1 to: 7123
pFN21AB7015 7123 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2278 2693 3026 3755 2278
Size: 415 333 729 5646
Fragments arranged by size:
5646 729 415 333
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4035 4271 4933 278
Size: 53 83 186 936 2499 236 662 2468
Fragments arranged by size:
2499 2468 936 662 236 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4014 4466 1937
Size: 2077 452 4594
Fragments arranged by size:
4594 2077 452
Acc65I G'GTAC_C
Cuts at: 4444 4444
Size: 7123
AclI AA'CG_TT
Cuts at: 1563 5250 5623 1563
Size: 3687 373 3063
Fragments arranged by size:
3687 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2350 2591 4145 5186 6570 6838 1632
Size: 718 241 1554 1041 1384 268 1917
Fragments arranged by size:
1917 1554 1384 1041 718 268 241
AfeI AGC'GCT
Cuts at: 2747 2747
Size: 7123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6926
AflIII A'CryG_T
Cuts at: 1203 3288 6022 6999 1203
Size: 2085 2734 977 1327
Fragments arranged by size:
2734 2085 1327 977
AgeI A'CCGG_T
Cuts at: 5954 5954
Size: 7123
AhdI GACnn_n'nnGTC
Cuts at: 3571 3787 4036 5852 3571
Size: 216 249 1816 4842
Fragments arranged by size:
4842 1816 249 216
AleI CACnn'nnGTG
Cuts at: 1166 2367 2438 4026 1166
Size: 1201 71 1588 4263
Fragments arranged by size:
4263 1588 1201 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2636 1184
Size: 1452 5671
AlwNI CAG_nnn'CTG
Cuts at: 2284 2293 2540 4866 6438 2284
Size: 9 247 2326 1572 2969
Fragments arranged by size:
2969 2326 1572 247 9
ApaI G_GGCC'C
Cuts at: 2126 2217 2277 2455 2903 3350 3415 3654 2126
Size: 91 60 178 448 447 65 239 5595
Fragments arranged by size:
5595 448 447 239 178 91 65 60
ApaLI G'TGCA_C
Cuts at: 3236 3929 5180 6336 3236
Size: 693 1251 1156 4023
Fragments arranged by size:
4023 1251 1156 693
ApoI r'AATT_y
Cuts at: 1493 4432 4658 4810 6919 1493
Size: 2939 226 152 2109 1697
Fragments arranged by size:
2939 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5677 160
Size: 872 4645 1606
Fragments arranged by size:
4645 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2195 2395 2471 3374 3408 3721
Size: 73 176 76 200 76 903 34 313
Cuts at: 3721 3758 3810 3921 4318 4448 1870
Size: 37 52 111 397 130 4545
Fragments arranged by size:
4545 903 397 313 200 176 130 111
76 76 73 52 37 34
BamHI G'GATC_C
Cuts at: 4453 4820 4453
Size: 367 6756
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2060 2248 2659 3632
Size: 325 217 627 222 51 188 411 973
Cuts at: 3632 3988 4297 4444 5899 618
Size: 356 309 147 1455 1842
Fragments arranged by size:
1842 1455 973 627 411 356 325 309
222 217 188 147 51
BanII G_rGCy'C
Cuts at: 1391 2126 2133 2217 2277 2455 2903 3227 3302
Size: 735 7 84 60 178 448 324 75
Cuts at: 3302 3350 3375 3415 3654 4082 4113 4415 4442
Size: 48 25 40 239 428 31 302 27
Cuts at: 4442 1391
Size: 4072
Fragments arranged by size:
4072 735 448 428 324 302 239 178
84 75 60 48 40 31 27 25
7
BbeI G_GCGC'C
Cuts at: 2013 2064 3636 3992 4301 2013
Size: 51 1572 356 309 4835
Fragments arranged by size:
4835 1572 356 309 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2146 2415 3959 928
Size: 971 247 269 1544 4092
Fragments arranged by size:
4092 1544 971 269 247
BbvCI CC'TCA_GC
Cuts at: 2427 3134 2427
Size: 707 6416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3365 3399 5314 5348 1903
Size: 34 1428 34 1915 34 3678
Fragments arranged by size:
3678 1915 1428 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5015 6225 5015
Size: 1210 5913
BclI T'GATC_A
Cuts at: 1548 4179 1548
Size: 2631 4492
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 2281 2504
Size: 107 122 71 631 605 382 227 223
Cuts at: 2504 2907 3027 5734 136
Size: 403 120 2707 1525
Fragments arranged by size:
2707 1525 631 605 403 382 227 223
122 120 107 71
BglII A'GATC_T
Cuts at: 3243 4062 7118 3243
Size: 819 3056 3248
Fragments arranged by size:
3248 3056 819
BlpI GC'TnA_GC
Cuts at: 2180 4540 2180
Size: 2360 4763
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2217 2253 2277 2455 2664 2903 3240
Size: 336 91 36 24 178 209 239 337
Cuts at: 3240 3350 3415 3654 3933 5184 6340 1790
Size: 110 65 239 279 1251 1156 2573
Fragments arranged by size:
2573 1251 1156 337 336 279 239 239
209 178 110 91 65 36 24
BmgBI CAC'GTC
Cuts at: 1318 2737 2815 3928 1318
Size: 1419 78 1113 4513
Fragments arranged by size:
4513 1419 1113 78
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4854 5812 442
Size: 416 53 83 399 24 3437 958 1753
Fragments arranged by size:
3437 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 4330 2992
Size: 1338 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2762 3299 4327 5783 1239
Size: 1523 537 1028 1456 2579
Fragments arranged by size:
2579 1523 1456 1028 537
Bpu10I CC'TnA_GC
Cuts at: 2427 3134 2427
Size: 707 6416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2852 2915 3245 5248 6113 6411 2852
Size: 63 330 2003 865 298 3564
Fragments arranged by size:
3564 2003 865 330 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5786 882
Size: 617 4287 2219
Fragments arranged by size:
4287 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2875 3661 4914 493
Size: 711 1671 786 1253 2702
Fragments arranged by size:
2702 1671 1253 786 711
BsaBI GATnn'nnATC
Cuts at: 3899 7117 3899
Size: 3218 3905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2061 3003 3633
Size: 53 83 186 936 477 51 942 630
Cuts at: 3633 3989 4032 4268 4298 4930 5312 5947 275
Size: 356 43 236 30 632 382 635 1451
Fragments arranged by size:
1451 942 936 635 632 630 477 382
356 236 186 83 53 51 43 30
BsaWI w'CCGG_w
Cuts at: 5556 5954 6228 6375 6786 5556
Size: 398 274 147 411 5893
Fragments arranged by size:
5893 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2297 2327 2371 2401 2687
Size: 30 165 30 894 30 44 30 286
Cuts at: 2687 2717 3230 3260 3815 3845 1178
Size: 30 513 30 555 30 4456
Fragments arranged by size:
4456 894 555 513 286 165 44 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2056 3098 3101 3776 4121 4339 1186
Size: 870 1042 3 675 345 218 3970
Fragments arranged by size:
3970 1042 870 675 345 218 3
BseYI C'CCAG_C
Cuts at: 2277 2533 2803 3628 4072 4337 4400 6326 6822
Size: 256 270 825 444 265 63 1926 496
Cuts at: 6822 2277
Size: 2578
Fragments arranged by size:
2578 1926 825 496 444 270 265 256
63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3622 4144 1163
Size: 573 1886 522 4142
Fragments arranged by size:
4142 1886 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2508 3967 4573 5334 6362 1540
Size: 275 186 507 1459 606 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 606 507 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 2931 3240 3302 3375 3933 4113 5184 5269
Size: 330 309 62 73 558 180 1071 85
Cuts at: 5269 6340 2601
Size: 1071 3384
Fragments arranged by size:
3384 1071 1071 558 330 309 180 85
73 62
BsmI GAATG_Cn'
Cuts at: 2302 4634 4727 2302
Size: 2332 93 4698
Fragments arranged by size:
4698 2332 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2133 2217 2253 2277 2455 2601
Size: 399 336 7 84 36 24 178 146
Cuts at: 2601 2664 2903 2931 3227 3240 3302 3350 3375
Size: 63 239 28 296 13 62 48 25
Cuts at: 3375 3415 3654 3933 4082 4113 4415 4442 5184
Size: 40 239 279 149 31 302 27 742
Cuts at: 5184 5269 6340 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 336 302 296 279
239 239 178 149 146 85 84 63
62 48 40 36 31 28 27 25
24 13 7
BspEI T'CCGG_A
Cuts at: 6786 6786
Size: 7123
BspHI T'CATG_A
Cuts at: 5012 6742 5012
Size: 1730 5393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2278 2693 3026 3633 3699 3755 4389 4478
Size: 1434 415 333 607 66 56 634 89
Cuts at: 4478 844
Size: 3489
Fragments arranged by size:
3489 1434 634 607 415 333 89 66
56
BsrBI CCG'CTC
Cuts at: 1394 1823 2108 2476 3259 5010 1394
Size: 429 285 368 783 1751 3507
Fragments arranged by size:
3507 1751 783 429 368 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 3874 5618 5792 65
Size: 1183 2626 1744 174 1396
Fragments arranged by size:
2626 1744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2057 2331 2503 2757 3966 5767 5954
Size: 110 247 274 172 254 1209 1801 187
Cuts at: 5954 1700
Size: 2869
Fragments arranged by size:
2869 1801 1209 274 254 247 187 172
110
BsrGI T'GTAC_A
Cuts at: 96 2835 4173 96
Size: 2739 1338 3046
Fragments arranged by size:
3046 2739 1338
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7123
BssSI C'ACGA_G
Cuts at: 2306 5183 6195 2306
Size: 2877 1012 3234
Fragments arranged by size:
3234 2877 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7123
BstBI TT'CG_AA
Cuts at: 5927 5927
Size: 7123
BstEII G'GTnAC_C
Cuts at: 1184 3686 3877 1184
Size: 2502 191 4430
Fragments arranged by size:
4430 2502 191
BstYI r'GATC_y
Cuts at: 1972 3243 4062 4453 4820 5205 5222 6674 6760
Size: 1271 819 391 367 385 17 1452 86
Cuts at: 6760 6772 7118 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1271 819 391 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3337 3993 4351 3337
Size: 656 358 6109
Fragments arranged by size:
6109 656 358
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2208 2590 2671 513
Size: 552 939 39 165 382 81 4965
Fragments arranged by size:
4965 939 552 382 165 81 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3307 487
Size: 683 56 2081 4303
Fragments arranged by size:
4303 2081 683 56
BtsI GCAGTG_nn'
Cuts at: 2639 2824 3121 4411 4639 5433 5453 2639
Size: 185 297 1290 228 794 20 4309
Fragments arranged by size:
4309 1290 794 297 228 185 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7088
DraI TTT'AAA
Cuts at: 4427 4427
Size: 7123
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6130 809
Size: 507 4814 1802
Fragments arranged by size:
4814 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2046 2055
Size: 54 1425 50 275 18 210 6 9
Cuts at: 2055 2134 2496 2505 2784 3357 3454 3678 3964
Size: 79 362 9 279 573 97 224 286
Cuts at: 3964 4324 4570 5459 7088 8
Size: 360 246 889 1629 43
Fragments arranged by size:
1629 1425 889 573 362 360 286 279
275 246 224 210 97 79 54 50
43 18 9 9 6
EagI C'GGCC_G
Cuts at: 1537 1812 2505 3964 4570 1537
Size: 275 693 1459 606 4090
Fragments arranged by size:
4090 1459 693 606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4118 5053 1353
Size: 2765 935 3423
Fragments arranged by size:
3423 2765 935
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3485 5710 6084 6230 387
Size: 1064 138 1896 2225 374 146 1280
Fragments arranged by size:
2225 1896 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3300 3373 4111 3300
Size: 73 738 6312
Fragments arranged by size:
6312 738 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2350 2591 2762 3299 4145 4327 5186
Size: 393 718 241 171 537 846 182 859
Cuts at: 5186 5783 6570 6838 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 859 846 787 718 597 537 393
268 241 182 171
EcoNI CCTnn'n_nnAGG
Cuts at: 3440 4213 3440
Size: 773 6350
EcoO109I rG'GnC_Cy
Cuts at: 2213 2273 2274 2319 2451 2452 3346 3347 3556
Size: 60 1 45 132 1 894 1 209
Cuts at: 3556 3651 3781 3853 4048 4243 2213
Size: 95 130 72 195 195 5093
Fragments arranged by size:
5093 894 209 195 195 132 130 95
72 60 45 1 1 1
EcoRI G'AATT_C
Cuts at: 4432 4810 4432
Size: 378 6745
FspI TGC'GCA
Cuts at: 3200 3320 5629 3200
Size: 120 2309 4694
Fragments arranged by size:
4694 2309 120
FspAI rTGC'GCAy
Cuts at: 3320 3320
Size: 7123
HaeII r_GCGC'y
Cuts at: 1678 2013 2064 2749 2946 3636 3992 4301 6270
Size: 335 51 685 197 690 356 309 1969
Cuts at: 6270 1678
Size: 2531
Fragments arranged by size:
2531 1969 690 685 356 335 309 197
51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2371 2375 2403 2407 3557
Size: 32 135 32 799 4 28 4 1150
Cuts at: 3557 3589 3773 3805 4022 4054 5764 5796 5838
Size: 32 184 32 217 32 1710 32 42
Cuts at: 5838 5870 1373
Size: 32 2626
Fragments arranged by size:
2626 1710 1150 799 217 184 135 42
32 32 32 32 32 32 32 28
4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4467 4713 669
Size: 988 281 2529 246 3079
Fragments arranged by size:
3079 2529 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7123
HpaI GTT'AAC
Cuts at: 4713 4713
Size: 7123
KasI G'GCGC_C
Cuts at: 2009 2060 3632 3988 4297 2009
Size: 51 1572 356 309 4835
Fragments arranged by size:
4835 1572 356 309 51
KpnI G_GTAC'C
Cuts at: 4448 4448
Size: 7123
MfeI C'AATT_G
Cuts at: 4722 4722
Size: 7123
MluI A'CGCG_T
Cuts at: 3288 6999 3288
Size: 3711 3412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3309 3327 3543 6237 6421 1305
Size: 2004 18 216 2694 184 2007
Fragments arranged by size:
2694 2007 2004 216 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 3359 4326 10
Size: 54 1425 343 216 1311 967 2807
Fragments arranged by size:
2807 1425 1311 967 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 2438 3167 4026 4100 4181 5081
Size: 648 1201 71 729 859 74 81 900
Cuts at: 5081 5440 5599 518
Size: 359 159 2042
Fragments arranged by size:
2042 1201 900 859 729 648 359 159
81 74 71
MspA1I CmG'CkG
Cuts at: 1606 2163 2281 2328 2338 2529 2540 2592 2673
Size: 557 118 47 10 191 11 52 81
Cuts at: 2673 4234 4539 5216 6364 6609 1606
Size: 1561 305 677 1148 245 2120
Fragments arranged by size:
2120 1561 1148 677 557 305 245 191
118 81 52 47 11 10
NaeI GCC'GGC
Cuts at: 2059 2333 2505 3968 2059
Size: 274 172 1463 5214
Fragments arranged by size:
5214 1463 274 172
NarI GG'CG_CC
Cuts at: 2010 2061 3633 3989 4298 2010
Size: 51 1572 356 309 4835
Fragments arranged by size:
4835 1572 356 309 51
NcoI C'CATG_G
Cuts at: 513 1065 2004 2208 513
Size: 552 939 204 5428
Fragments arranged by size:
5428 939 552 204
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7123
NgoMIV G'CCGG_C
Cuts at: 2057 2331 2503 3966 2057
Size: 274 172 1463 5214
Fragments arranged by size:
5214 1463 274 172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7123
NotI GC'GGCC_GC
Cuts at: 4570 4570
Size: 7123
NspI r_CATG'y
Cuts at: 1132 1229 4202 4481 6026 1132
Size: 97 2973 279 1545 2229
Fragments arranged by size:
2973 2229 1545 279 97
PasI CC'CwG_GG
Cuts at: 1348 3424 1348
Size: 2076 5047
PciI A'CATG_T
Cuts at: 6022 6022
Size: 7123
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2223 2540 2565 4141 1108
Size: 1115 317 25 1576 4090
Fragments arranged by size:
4090 1576 1115 317 25
PmeI GTTT'AAAC
Cuts at: 4427 4427
Size: 7123
PmlI CAC'GTG
Cuts at: 2875 2875
Size: 7123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5192 5224 6705 6737 5192
Size: 32 1481 32 5578
Fragments arranged by size:
5578 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3556 3781 3853 4048 4243 3556
Size: 225 72 195 195 6436
Fragments arranged by size:
6436 225 195 195 72
PshAI GACnn'nnGTC
Cuts at: 1613 3271 1613
Size: 1658 5465
PsiI TTA'TAA
Cuts at: 4693 4693
Size: 7123
PspOMI G'GGCC_C
Cuts at: 2122 2213 2273 2451 2899 3346 3411 3650 2122
Size: 91 60 178 448 447 65 239 5595
Fragments arranged by size:
5595 448 447 239 178 91 65 60
PspXI vC'TCGA_Gb
Cuts at: 3374 3374
Size: 7123
PstI C_TGCA'G
Cuts at: 830 2289 2772 4475 830
Size: 1459 483 1703 3478
Fragments arranged by size:
3478 1703 1459 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7123
PvuII CAG'CTG
Cuts at: 2328 4234 2328
Size: 1906 5217
RsrII CG'GwC_CG
Cuts at: 2668 2668
Size: 7123
SacI G_AGCT'C
Cuts at: 3302 3375 4113 3302
Size: 73 738 6312
Fragments arranged by size:
6312 738 73
SacII CC_GC'GG
Cuts at: 2593 2674 2593
Size: 81 7042
SalI G'TCGA_C
Cuts at: 1936 4465 1936
Size: 2529 4594
SapI GCTCTTCn'nnn_
Cuts at: 4118 4118
Size: 7123
SbfI CC_TGCA'GG
Cuts at: 2772 4475 2772
Size: 1703 5420
ScaI AGT'ACT
Cuts at: 3824 4313 5371 7083 3824
Size: 489 1058 1712 3864
Fragments arranged by size:
3864 1712 1058 489
SexAI A'CCwGG_T
Cuts at: 3567 3783 3801 3567
Size: 216 18 6889
Fragments arranged by size:
6889 216 18
SfcI C'TryA_G
Cuts at: 826 1045 2285 2323 2768 4471 5606 6287 6478
Size: 219 1240 38 445 1703 1135 681 191
Cuts at: 6478 826
Size: 1471
Fragments arranged by size:
1703 1471 1240 1135 681 445 219 191
38
SfiI GGCCn_nnn'nGGCC
Cuts at: 2054 2504 2054
Size: 450 6673
SfoI GGC'GCC
Cuts at: 2011 2062 3634 3990 4299 2011
Size: 51 1572 356 309 4835
Fragments arranged by size:
4835 1572 356 309 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7123
SmaI CCC'GGG
Cuts at: 1872 2121 2197 3723 3812 3923 4320 4450 1872
Size: 249 76 1526 89 111 397 130 4545
Fragments arranged by size:
4545 1526 397 249 130 111 89 76
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2867 2930 3260 3374 5227 6128
Size: 197 926 924 63 330 114 1853 901
Cuts at: 6128 6390 820
Size: 262 1553
Fragments arranged by size:
1853 1553 926 924 901 330 262 197
114 63
SnaBI TAC'GTA
Cuts at: 493 4914 493
Size: 4421 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7123
SphI G_CATG'C
Cuts at: 1132 4202 4481 1132
Size: 3070 279 3774
Fragments arranged by size:
3774 3070 279
SspI AAT'ATT
Cuts at: 5 52 5047 5
Size: 47 4995 2081
Fragments arranged by size:
4995 2081 47
StuI AGG'CCT
Cuts at: 1517 3219 3998 1517
Size: 1702 779 4642
Fragments arranged by size:
4642 1702 779
StyI C'CwwG_G
Cuts at: 513 1065 2004 2076 2208 3436 3681 4562 513
Size: 552 939 72 132 1228 245 881 3074
Fragments arranged by size:
3074 1228 939 881 552 245 132 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3896 4023 4414 5150 5167 5320 5505
Size: 1255 2316 127 391 736 17 153 185
Cuts at: 5505 5950 325
Size: 445 1498
Fragments arranged by size:
2316 1498 1255 736 445 391 185 153
127 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2835 3012 3822
Size: 274 80 33 51 1443 858 177 810
Cuts at: 3822 4173 4311 5369 7081 96
Size: 351 138 1058 1712 138
Fragments arranged by size:
1712 1443 1058 858 810 351 274 177
138 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2297 2329 3230 3262 7083 7115 2297
Size: 32 901 32 3821 32 2305
Fragments arranged by size:
3821 2305 901 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4010 4253 1316
Size: 2694 243 4186
Fragments arranged by size:
4186 2694 243
XbaI T'CTAG_A
Cuts at: 4459 4459
Size: 7123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3217 3310 3217
Size: 93 7030
XhoI C'TCGA_G
Cuts at: 1943 3374 1943
Size: 1431 5692
XmaI C'CCGG_G
Cuts at: 1870 2119 2195 3721 3810 3921 4318 4448 1870
Size: 249 76 1526 89 111 397 130 4545
Fragments arranged by size:
4545 1526 397 249 130 111 89 76
XmnI GAAnn'nnTTC
Cuts at: 5252 5252
Size: 7123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4033 4269 4931 276
Size: 53 83 186 936 2499 236 662 2468
Fragments arranged by size:
2499 2468 936 662 236 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII EcoRV FalI FseI NruI NsiI PacI
PfoI PsrI SanDI SrfI SwaI