Restriction map for
pFN21AB7014
(Circular) (Six-base) MAPSORT of: pFN21AB7014.seq Check: 2105 from: 1 to: 10447
pFN21AB7014 10447 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2074 3565 4609 5602 5717 5749 7708 2074
Size: 1491 1044 993 115 32 1959 4813
Fragments arranged by size:
4813 1959 1491 1044 993 115 32
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6777 6875 8257 278
Size: 53 83 186 936 5241 98 1382 2468
Fragments arranged by size:
5241 2468 1382 936 186 98 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 7790 1937
Size: 889 4964 4594
Fragments arranged by size:
4964 4594 889
Acc65I G'GTAC_C
Cuts at: 7768 7768
Size: 10447
AclI AA'CG_TT
Cuts at: 1563 8574 8947 1563
Size: 7011 373 3063
Fragments arranged by size:
7011 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5567 7147 8510 9894 10162 1632
Size: 3935 1580 1363 1384 268 1917
Fragments arranged by size:
3935 1917 1580 1384 1363 268
AfeI AGC'GCT
Cuts at: 3722 4922 5128 3722
Size: 1200 206 9041
Fragments arranged by size:
9041 1200 206
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10250
AflIII A'CryG_T
Cuts at: 1203 2700 3612 4110 4161 7209 9346 10323 1203
Size: 1497 912 498 51 3048 2137 977 1327
Fragments arranged by size:
3048 2137 1497 1327 977 912 498 51
AgeI A'CCGG_T
Cuts at: 9278 9278
Size: 10447
AhdI GACnn_n'nnGTC
Cuts at: 2764 9176 2764
Size: 6412 4035
AleI CACnn'nnGTG
Cuts at: 1166 4931 6275 1166
Size: 3765 1344 5338
Fragments arranged by size:
5338 3765 1344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6776 7307 1184
Size: 5592 531 4324
Fragments arranged by size:
5592 4324 531
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3053 3085 5283 5315 6425 6457 3053
Size: 32 2198 32 1110 32 7043
Fragments arranged by size:
7043 2198 1110 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2202 2479 3301 3678 4186 4638 6142 7699 8190
Size: 277 822 377 508 452 1504 1557 491
Cuts at: 8190 9762 2202
Size: 1572 2887
Fragments arranged by size:
2887 1572 1557 1504 822 508 491 452
377 277
ApaI G_GGCC'C
Cuts at: 2308 2321 4682 6485 2308
Size: 13 2361 1803 6270
Fragments arranged by size:
6270 2361 1803 13
ApaLI G'TGCA_C
Cuts at: 4114 8504 9660 4114
Size: 4390 1156 4901
Fragments arranged by size:
4901 4390 1156
ApoI r'AATT_y
Cuts at: 1493 2930 4363 7756 7982 8134 10243 1493
Size: 1437 1433 3393 226 152 2109 1697
Fragments arranged by size:
3393 2109 1697 1437 1433 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9001 160
Size: 872 7969 1606
Fragments arranged by size:
7969 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10447
AvaI C'yCGr_G
Cuts at: 1870 1943 3924 4303 4670 4681 5108 5222 7772
Size: 73 1981 379 367 11 427 114 2550
Cuts at: 7772 1870
Size: 4545
Fragments arranged by size:
4545 2550 1981 427 379 367 114 73
11
BamHI G'GATC_C
Cuts at: 3900 7777 8144 3900
Size: 3877 367 6203
Fragments arranged by size:
6203 3877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3586 3934 4985 5094 5267
Size: 325 217 627 1799 348 1051 109 173
Cuts at: 5267 5686 6005 6610 6732 6859 7007 7020 7768
Size: 419 319 605 122 127 148 13 748
Cuts at: 7768 9223 618
Size: 1455 1842
Fragments arranged by size:
1842 1799 1455 1051 748 627 605 419
348 325 319 217 173 148 127 122
109 13
BanII G_rGCy'C
Cuts at: 1391 2308 2321 3717 4682 4961 5120 6485 7184
Size: 917 13 1396 965 279 159 1365 699
Cuts at: 7184 7766 1391
Size: 582 4072
Fragments arranged by size:
4072 1396 1365 965 917 699 582 279
159 13
BbeI G_GCGC'C
Cuts at: 3938 5690 6736 3938
Size: 1752 1046 7649
Fragments arranged by size:
7649 1752 1046
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4021 4746 928
Size: 971 2122 725 6629
Fragments arranged by size:
6629 2122 971 725
BbvCI CC'TCA_GC
Cuts at: 2136 3051 3533 6917 2136
Size: 915 482 3384 5666
Fragments arranged by size:
5666 3384 915 482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2611 2645 2675 2709 4566 4583 4600
Size: 34 674 34 30 34 1857 17 17
Cuts at: 4600 4617 8638 8672 1903
Size: 17 4021 34 3678
Fragments arranged by size:
4021 3678 1857 674 34 34 34 34
30 17 17 17
BciVI GTATCCnnnnn_n'
Cuts at: 8339 9549 8339
Size: 1210 9237
BclI T'GATC_A
Cuts at: 1548 6984 7317 1548
Size: 5436 333 4678
Fragments arranged by size:
5436 4678 333
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2376 3018 5532
Size: 107 122 71 631 605 704 642 2514
Cuts at: 5532 9058 136
Size: 3526 1525
Fragments arranged by size:
3526 2514 1525 704 642 631 605 122
107 71
BglII A'GATC_T
Cuts at: 4200 7089 7620 10442 4200
Size: 2889 531 2822 4205
Fragments arranged by size:
4205 2889 2822 531
BlpI GC'TnA_GC
Cuts at: 7183 7566 7864 7183
Size: 383 298 9766
Fragments arranged by size:
9766 383 298
Bme1580I G_kGCm'C
Cuts at: 1790 2308 2321 2624 3028 3311 4118 4487 4682
Size: 518 13 303 404 283 807 369 195
Cuts at: 4682 4855 4988 6485 6690 6864 7124 8508 9664
Size: 173 133 1497 205 174 260 1384 1156
Cuts at: 9664 1790
Size: 2573
Fragments arranged by size:
2573 1497 1384 1156 807 518 404 369
303 283 260 205 195 174 173 133
13
BmgBI CAC'GTC
Cuts at: 1318 2311 2895 3064 3615 4744 7264 1318
Size: 993 584 169 551 1129 2520 4501
Fragments arranged by size:
4501 2520 1129 993 584 551 169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 2513 5709
Size: 416 53 83 399 24 795 301 3196
Cuts at: 5709 8178 9136 442
Size: 2469 958 1753
Fragments arranged by size:
3196 2469 1753 958 795 416 399 301
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10447
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7642 7642
Size: 10447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3908 4166 4330 5092 5534 5561 5891 5942
Size: 2669 258 164 762 442 27 330 51
Cuts at: 5942 6203 6338 6746 7019 7688 7733 9107 1239
Size: 261 135 408 273 669 45 1374 2579
Fragments arranged by size:
2669 2579 1374 762 669 442 408 330
273 261 258 164 135 51 45 27
Bpu10I CC'TnA_GC
Cuts at: 2136 3008 3051 3143 3533 6917 7586 7739 2136
Size: 872 43 92 390 3384 669 153 4844
Fragments arranged by size:
4844 3384 872 669 390 153 92 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 4502 4643 5555 5876 6068 6266 6401 8572
Size: 1773 141 912 321 192 198 135 2171
Cuts at: 8572 9437 9735 2729
Size: 865 298 3441
Fragments arranged by size:
3441 2171 1773 912 865 321 298 198
192 141 135
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3516 3951 5825 7087 7148 9110 882
Size: 617 2017 435 1874 1262 61 1962 2219
Fragments arranged by size:
2219 2017 1962 1874 1262 617 435 61
BsaAI yAC'GTr
Cuts at: 493 1204 4111 4207 8238 493
Size: 711 2907 96 4031 2702
Fragments arranged by size:
4031 2907 2702 711 96
BsaBI GATnn'nnATC
Cuts at: 4997 10441 4997
Size: 5444 5003
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3329 3693 3843 3935
Size: 53 83 186 936 1796 364 150 92
Cuts at: 3935 4836 4860 5687 6492 6733 6774 6872 7368
Size: 901 24 827 805 241 41 98 496
Cuts at: 7368 8254 8636 9271 275
Size: 886 382 635 1451
Fragments arranged by size:
1796 1451 936 901 886 827 805 635
496 382 364 241 186 150 98 92
83 53 41 24
BsaWI w'CCGG_w
Cuts at: 5091 6030 8880 9278 9552 9699 10110 5091
Size: 939 2850 398 274 147 411 5428
Fragments arranged by size:
5428 2850 939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4781 4811 4826 4856 5444
Size: 30 165 30 3378 30 15 30 588
Cuts at: 5444 5468 5474 5498 6425 6455 6872 6902 7394
Size: 24 6 24 927 30 417 30 492
Cuts at: 7394 7424 7430 7460 1178
Size: 30 6 30 4165
Fragments arranged by size:
4165 3378 927 588 492 417 165 30
30 30 30 30 30 30 30 24
24 15 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 6886 1186
Size: 1516 4184 4747
Fragments arranged by size:
4747 4184 1516
BseYI C'CCAG_C
Cuts at: 2017 2049 2354 3040 3235 3717 5157 5413 5434
Size: 32 305 686 195 482 1440 256 21
Cuts at: 5434 5552 6428 6696 7429 7491 9650 10146 2017
Size: 118 876 268 733 62 2159 496 2318
Fragments arranged by size:
2318 2159 1440 876 733 686 496 482
305 268 256 195 118 62 32 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 2567 3014 3626 3653 3770 3782
Size: 573 645 186 447 612 27 117 12
Cuts at: 3782 4624 4798 5580 6119 7565 1163
Size: 842 174 782 539 1446 4045
Fragments arranged by size:
4045 1446 842 782 645 612 573 539
447 186 174 117 27 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3022 5046 5426 6214 7897 8658
Size: 275 186 1021 2024 380 788 1683 761
Cuts at: 8658 9686 1540
Size: 1028 2301
Fragments arranged by size:
2301 2024 1683 1028 1021 788 761 380
275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2091 2532 2577 2886 4118 5475 5631 6054
Size: 57 441 45 309 1232 1357 156 423
Cuts at: 6054 6084 6282 7425 8508 8593 9664 2034
Size: 30 198 1143 1083 85 1071 2817
Fragments arranged by size:
2817 1357 1232 1143 1083 1071 441 423
309 198 156 85 57 45 30
BsiWI C'GTAC_G
Cuts at: 2940 2940
Size: 10447
BsmI GAATG_Cn'
Cuts at: 5948 6604 7958 8051 5948
Size: 656 1354 93 8344
Fragments arranged by size:
8344 1354 656 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4667 5660 4667
Size: 993 9454
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2091 2308 2321 2532 2577 2624
Size: 399 244 57 217 13 211 45 47
Cuts at: 2624 2886 3028 3311 3717 4118 4487 4682 4855
Size: 262 142 283 406 401 369 195 173
Cuts at: 4855 4961 4988 5120 5475 5631 6054 6084 6282
Size: 106 27 132 355 156 423 30 198
Cuts at: 6282 6485 6690 6864 7124 7184 7425 7766 8508
Size: 203 205 174 260 60 241 341 742
Cuts at: 8508 8593 9664 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 406 401 399 369
355 341 283 262 260 244 241 217
211 205 203 198 195 174 173 156
142 132 106 85 60 57 47 45
30 27 13
BspEI T'CCGG_A
Cuts at: 10110 10110
Size: 10447
BspHI T'CATG_A
Cuts at: 6237 8336 10066 6237
Size: 2099 1730 6618
Fragments arranged by size:
6618 2099 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 3565 3744 4260 4410 4609 5602 5717
Size: 1230 1491 179 516 150 199 993 115
Cuts at: 5717 5749 7708 7802 844
Size: 32 1959 94 3489
Fragments arranged by size:
3489 1959 1491 1230 993 516 199 179
150 115 94 32
BsrBI CCG'CTC
Cuts at: 1394 1823 3912 5422 7005 8334 1394
Size: 429 2089 1510 1583 1329 3507
Fragments arranged by size:
3507 2089 1583 1510 1329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2577 3778 8942 9116 65
Size: 1183 1329 1201 5164 174 1396
Fragments arranged by size:
5164 1396 1329 1201 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2876 3012 3407 3465 5052 6008 6613
Size: 110 1066 136 395 58 1587 956 605
Cuts at: 6613 9091 9278 1700
Size: 2478 187 2869
Fragments arranged by size:
2869 2478 1587 1066 956 605 395 187
136 110 58
BsrGI T'GTAC_A
Cuts at: 96 2799 5346 96
Size: 2703 2547 5197
Fragments arranged by size:
5197 2703 2547
BssHII G'CGCG_C
Cuts at: 1925 6749 1925
Size: 4824 5623
BssSI C'ACGA_G
Cuts at: 5871 5981 7616 8507 9519 5871
Size: 110 1635 891 1012 6799
Fragments arranged by size:
6799 1635 1012 891 110
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3748 4638 6160 7699 794
Size: 2954 890 1522 1539 3542
Fragments arranged by size:
3542 2954 1539 1522 890
BstBI TT'CG_AA
Cuts at: 4737 9251 4737
Size: 4514 5933
BstEII G'GTnAC_C
Cuts at: 1184 6640 7061 1184
Size: 5456 421 4570
Fragments arranged by size:
5456 4570 421
BstXI CCAn_nnnn'nTGG
Cuts at: 4237 5998 4237
Size: 1761 8686
BstYI r'GATC_y
Cuts at: 1972 2748 3435 3900 4182 4200 4653 5088 7089
Size: 776 687 465 282 18 453 435 2001
Cuts at: 7089 7248 7620 7653 7777 8144 8529 8546 9998
Size: 159 372 33 124 367 385 17 1452
Cuts at: 9998 10084 10096 10442 1972
Size: 86 12 346 1977
Fragments arranged by size:
2001 1977 1452 776 687 465 453 435
385 372 367 346 282 159 124 86
33 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 5172 5210 6606 5172
Size: 38 1396 9013
Fragments arranged by size:
9013 1396 38
BtgI C'CryG_G
Cuts at: 513 1065 2072 2374 3190 3215 3366 4966 5361
Size: 552 1007 302 816 25 151 1600 395
Cuts at: 5361 6214 6711 7066 513
Size: 853 497 355 3894
Fragments arranged by size:
3894 1600 1007 853 816 552 497 395
355 302 151 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7501 487
Size: 683 56 6275 3433
Fragments arranged by size:
6275 3433 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2294 3749 3904 4361 4582 5032 5753 7124
Size: 29 1455 155 457 221 450 721 1371
Cuts at: 7124 7963 8757 8777 2265
Size: 839 794 20 3935
Fragments arranged by size:
3935 1455 1371 839 794 721 457 450
221 155 29 20
ClaI AT'CG_AT
Cuts at: 1554 2631 3264 1554
Size: 1077 633 8737
Fragments arranged by size:
8737 1077 633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5224 5259 6769 6804 529
Size: 35 4660 35 1510 35 4172
Fragments arranged by size:
4660 4172 1510 35 35 35
DraI TTT'AAA
Cuts at: 7751 7751
Size: 10447
DraIII CAC_nnn'GTG
Cuts at: 2221 4126 6079 7027 2221
Size: 1905 1953 948 5641
Fragments arranged by size:
5641 1953 1905 948
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6773 6841 9454 809
Size: 507 5457 68 2613 1802
Fragments arranged by size:
5457 2613 1802 507 68
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2679 3019
Size: 54 1425 50 275 18 363 486 340
Cuts at: 3019 3421 3463 3895 4239 4554 5050 5277 5385
Size: 402 42 432 344 315 496 227 108
Cuts at: 5385 5423 6945 7110 7197 7894 8783 10412 8
Size: 38 1522 165 87 697 889 1629 43
Fragments arranged by size:
1629 1522 1425 889 697 496 486 432
402 363 344 340 315 275 227 165
108 87 54 50 43 42 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 3019 5423 7894 1537
Size: 275 1207 2404 2471 4090
Fragments arranged by size:
4090 2471 2404 1207 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 2618 4298 6797 7274 8377 1353
Size: 1148 117 1680 2499 477 1103 3423
Fragments arranged by size:
3423 2499 1680 1148 1103 477 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 5823 6865 9034 9408 9554
Size: 1064 138 2321 1913 1042 2169 374 146
Cuts at: 9554 387
Size: 1280
Fragments arranged by size:
2321 2169 1913 1280 1064 1042 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3908 4166 4330 5092 5534 5561 5567
Size: 393 2276 258 164 762 442 27 6
Cuts at: 5567 5891 5942 6203 6338 6746 7019 7147 7688
Size: 324 51 261 135 408 273 128 541
Cuts at: 7688 7733 8510 9107 9894 10162 1239
Size: 45 777 597 787 268 1524
Fragments arranged by size:
2276 1524 787 777 762 597 541 442
408 393 324 273 268 261 258 164
135 128 51 45 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2993 3050 4868 4977 6524 7670 2993
Size: 57 1818 109 1547 1146 5770
Fragments arranged by size:
5770 1818 1547 1146 109 57
EcoO109I rG'GnC_Cy
Cuts at: 2046 2206 2318 3643 3796 5197 5247 6481 6482
Size: 160 112 1325 153 1401 50 1234 1
Cuts at: 6482 6843 2046
Size: 361 5650
Fragments arranged by size:
5650 1401 1325 1234 361 160 153 112
50 1
EcoRI G'AATT_C
Cuts at: 4363 7756 8134 4363
Size: 3393 378 6676
Fragments arranged by size:
6676 3393 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5971 6172 5971
Size: 201 10246
FseI GG_CCGG'CC
Cuts at: 3469 5056 3469
Size: 1587 8860
FspI TGC'GCA
Cuts at: 5399 6941 8953 5399
Size: 1542 2012 6893
Fragments arranged by size:
6893 2012 1542
HaeII r_GCGC'y
Cuts at: 1678 2457 3724 3938 4564 4924 5130 5690 6736
Size: 779 1267 214 626 360 206 560 1046
Cuts at: 6736 7611 9594 1678
Size: 875 1983 2531
Fragments arranged by size:
2531 1983 1267 1046 875 779 626 560
360 214 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2717 2749 2750
Size: 32 135 32 1052 32 61 32 1
Cuts at: 2750 2782 3513 3545 4826 4858 6830 6862 7394
Size: 32 731 32 1281 32 1972 32 532
Cuts at: 7394 7426 7430 7462 7543 7575 9088 9120 9162
Size: 32 4 32 81 32 1513 32 42
Cuts at: 9162 9194 1373
Size: 32 2626
Fragments arranged by size:
2626 1972 1513 1281 1052 731 532 135
81 61 42 32 32 32 32 32
32 32 32 32 32 32 32 32
4 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2401 3847 4072 4159 7791 8037
Size: 988 281 463 1446 225 87 3632 246
Cuts at: 8037 669
Size: 3079
Fragments arranged by size:
3632 3079 1446 988 463 281 246 225
87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10447
HpaI GTT'AAC
Cuts at: 8037 8037
Size: 10447
KasI G'GCGC_C
Cuts at: 3934 5686 6732 3934
Size: 1752 1046 7649
Fragments arranged by size:
7649 1752 1046
KpnI G_GTAC'C
Cuts at: 7772 7772
Size: 10447
MfeI C'AATT_G
Cuts at: 8046 8046
Size: 10447
MluI A'CGCG_T
Cuts at: 10323 10323
Size: 10447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5643 6321 6915 7473 9561 9745 1305
Size: 4338 678 594 558 2088 184 2007
Fragments arranged by size:
4338 2088 2007 678 594 558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2681 5279 5387 7112 7199
Size: 54 1425 343 849 2598 108 1725 87
Cuts at: 7199 10
Size: 3258
Fragments arranged by size:
3258 2598 1725 1425 849 343 108 87
54
MslI CAynn'nnrTG
Cuts at: 518 1166 4931 5360 6047 6275 6980 8405 8764
Size: 648 3765 429 687 228 705 1425 359
Cuts at: 8764 8923 518
Size: 159 2042
Fragments arranged by size:
3765 2042 1425 705 687 648 429 359
228 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2074 2857 3040 3754 3839 3964 4566
Size: 459 9 783 183 714 85 125 602
Cuts at: 4566 5241 6139 6216 6289 6439 6700 6713 7068
Size: 675 898 77 73 150 261 13 355
Cuts at: 7068 7195 7696 7863 8540 9688 9933 1606
Size: 127 501 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 898 783 714 677 675 602
501 459 355 261 245 183 167 150
127 125 85 77 73 13 9
NaeI GCC'GGC
Cuts at: 2878 3014 3409 3467 5054 6010 2878
Size: 136 395 58 1587 956 7315
Fragments arranged by size:
7315 1587 956 395 136 58
NarI GG'CG_CC
Cuts at: 3935 5687 6733 3935
Size: 1752 1046 7649
Fragments arranged by size:
7649 1752 1046
NcoI C'CATG_G
Cuts at: 513 1065 5361 513
Size: 552 4296 5599
Fragments arranged by size:
5599 4296 552
NdeI CA'TA_TG
Cuts at: 387 6037 387
Size: 5650 4797
NgoMIV G'CCGG_C
Cuts at: 2876 3012 3407 3465 5052 6008 2876
Size: 136 395 58 1587 956 7315
Fragments arranged by size:
7315 1587 956 395 136 58
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10447
NotI GC'GGCC_GC
Cuts at: 7894 7894
Size: 10447
NspI r_CATG'y
Cuts at: 1132 1229 2131 2704 6937 7213 7805 9350 1132
Size: 97 902 573 4233 276 592 1545 2229
Fragments arranged by size:
4233 2229 1545 902 592 573 276 97
PasI CC'CwG_GG
Cuts at: 1348 2151 2461 3085 3301 1348
Size: 803 310 624 216 8494
Fragments arranged by size:
8494 803 624 310 216
PciI A'CATG_T
Cuts at: 2700 7209 9346 2700
Size: 4509 2137 3801
Fragments arranged by size:
4509 3801 2137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2202 2221 2380 3040 3301 3967 7017 1108
Size: 1094 19 159 660 261 666 3050 4538
Fragments arranged by size:
4538 3050 1094 666 660 261 159 19
PfoI T'CCnGG_A
Cuts at: 3885 4143 5069 7221 3885
Size: 258 926 2152 7111
Fragments arranged by size:
7111 2152 926 258
PmeI GTTT'AAAC
Cuts at: 7751 7751
Size: 10447
PmlI CAC'GTG
Cuts at: 4111 4111
Size: 10447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6425 6457 8516 8548 10029 10061 6425
Size: 32 2059 32 1481 32 6811
Fragments arranged by size:
6811 2059 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3643 3796 5197 5247 6843 3643
Size: 153 1401 50 1596 7247
Fragments arranged by size:
7247 1596 1401 153 50
PshAI GACnn'nnGTC
Cuts at: 1613 4746 1613
Size: 3133 7314
PsiI TTA'TAA
Cuts at: 8017 8017
Size: 10447
PspOMI G'GGCC_C
Cuts at: 2304 2317 4678 6481 2304
Size: 13 2361 1803 6270
Fragments arranged by size:
6270 2361 1803 13
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 10415
PstI C_TGCA'G
Cuts at: 830 2085 2844 3261 3744 3813 4043 4356 5760
Size: 1255 759 417 483 69 230 313 1404
Cuts at: 5760 5904 5970 6252 6765 7131 7799 830
Size: 144 66 282 513 366 668 3478
Fragments arranged by size:
3478 1404 1255 759 668 513 483 417
366 313 282 230 144 69 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10447
PvuII CAG'CTG
Cuts at: 2857 3040 3754 3839 6139 6289 2857
Size: 183 714 85 2300 150 7015
Fragments arranged by size:
7015 2300 714 183 150 85
SacII CC_GC'GG
Cuts at: 2075 6217 6714 7069 2075
Size: 4142 497 355 5453
Fragments arranged by size:
5453 4142 497 355
SalI G'TCGA_C
Cuts at: 1936 7789 1936
Size: 5853 4594
SanDI GG'GwC_CC
Cuts at: 3643 5247 3643
Size: 1604 8843
SapI GCTCTTCn'nnn_
Cuts at: 2618 7274 2618
Size: 4656 5791
SbfI CC_TGCA'GG
Cuts at: 2085 6252 6765 7799 2085
Size: 4167 513 1034 4733
Fragments arranged by size:
4733 4167 1034 513
ScaI AGT'ACT
Cuts at: 2389 2648 8695 10407 2389
Size: 259 6047 1712 2429
Fragments arranged by size:
6047 2429 1712 259
SexAI A'CCwGG_T
Cuts at: 6828 7023 6828
Size: 195 10252
SfcI C'TryA_G
Cuts at: 826 1045 2081 2840 3257 3740 3809 4039 4352
Size: 219 1036 759 417 483 69 230 313
Cuts at: 4352 5756 5900 5966 6248 6761 7127 7795 8930
Size: 1404 144 66 282 513 366 668 1135
Cuts at: 8930 9611 9802 826
Size: 681 191 1471
Fragments arranged by size:
1471 1404 1135 1036 759 681 668 513
483 417 366 313 282 230 219 191
144 69 66
SfoI GGC'GCC
Cuts at: 3936 5688 6734 3936
Size: 1752 1046 7649
Fragments arranged by size:
7649 1752 1046
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10447
SgrAI Cr'CCGG_yG
Cuts at: 1700 2876 1700
Size: 1176 9271
SmaI CCC'GGG
Cuts at: 1872 3926 4305 5110 7774 1872
Size: 2054 379 805 2664 4545
Fragments arranged by size:
4545 2664 2054 805 379
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 4517 4658 5570 5891 6083
Size: 197 926 801 1773 141 912 321 192
Cuts at: 6083 6281 6416 8551 9452 9714 820
Size: 198 135 2135 901 262 1553
Fragments arranged by size:
2135 1773 1553 926 912 901 801 321
262 198 197 192 141 135
SnaBI TAC'GTA
Cuts at: 493 8238 493
Size: 7745 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10447
SphI G_CATG'C
Cuts at: 1132 6937 7805 1132
Size: 5805 868 3774
Fragments arranged by size:
5805 3774 868
SspI AAT'ATT
Cuts at: 5 52 8371 5
Size: 47 8319 2081
Fragments arranged by size:
8319 2081 47
StuI AGG'CCT
Cuts at: 1517 3815 1517
Size: 2298 8149
StyI C'CwwG_G
Cuts at: 513 1065 2042 3990 5361 6651 6705 7116 7467
Size: 552 977 1948 1371 1290 54 411 351
Cuts at: 7467 7886 513
Size: 419 3074
Fragments arranged by size:
3074 1948 1371 1290 977 552 419 411
351 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2228 2495 2641 3581 4930 5032 6659
Size: 1255 648 267 146 940 1349 102 1627
Cuts at: 6659 7297 7483 8474 8491 8644 8829 9274 325
Size: 638 186 991 17 153 185 445 1498
Fragments arranged by size:
1627 1498 1349 1255 991 940 648 638
445 267 186 185 153 146 102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2387 2646 2799
Size: 274 80 33 51 1443 410 259 153
Cuts at: 2799 3609 4797 5181 5346 5763 6450 7206 7641
Size: 810 1188 384 165 417 687 756 435
Cuts at: 7641 8693 10405 96
Size: 1052 1712 138
Fragments arranged by size:
1712 1443 1188 1052 810 756 687 435
417 410 384 274 259 165 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2522 2554 4446 4478 4536 4568 5068 5100 6713
Size: 32 1892 32 58 32 500 32 1613
Cuts at: 6713 6745 10407 10439 2522
Size: 32 3662 32 2530
Fragments arranged by size:
3662 2530 1892 1613 500 58 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2396 3527 4025 6358 6638 1316
Size: 1080 1131 498 2333 280 5125
Fragments arranged by size:
5125 2333 1131 1080 498 280
XbaI T'CTAG_A
Cuts at: 7783 7783
Size: 10447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2614 5536 2614
Size: 2922 7525
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10447
XmaI C'CCGG_G
Cuts at: 1870 3924 4303 5108 7772 1870
Size: 2054 379 805 2664 4545
Fragments arranged by size:
4545 2664 2054 805 379
XmnI GAAnn'nnTTC
Cuts at: 6155 8576 6155
Size: 2421 8026
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6775 6873 8255 276
Size: 53 83 186 936 5241 98 1382 2468
Fragments arranged by size:
5241 2468 1382 936 186 98 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I EcoICRI EcoRV FspAI
NruI NsiI PacI PspXI RsrII SacI SfiI SrfI
SwaI