Restriction map for
pFN21AB7011
(Circular) (Six-base) MAPSORT of: pFN21AB7011.seq Check: 8653 from: 1 to: 7840
pFN21AB7011 7840 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2410 2410
Size: 7840
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4359 5650 278
Size: 53 83 186 936 2823 1291 2468
Fragments arranged by size:
2823 2468 1291 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3650 5022 5183 1937
Size: 1713 1372 161 4594
Fragments arranged by size:
4594 1713 1372 161
Acc65I G'GTAC_C
Cuts at: 2403 5161 2403
Size: 2758 5082
AclI AA'CG_TT
Cuts at: 1563 5967 6340 1563
Size: 4404 373 3063
Fragments arranged by size:
4404 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 3692 3908 4117 4768 5014 5903 7287
Size: 462 1598 216 209 651 246 889 1384
Cuts at: 7287 7555 1632
Size: 268 1917
Fragments arranged by size:
1917 1598 1384 889 651 462 268 246
216 209
AfeI AGC'GCT
Cuts at: 2828 2828
Size: 7840
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7643
AflIII A'CryG_T
Cuts at: 1203 2453 4743 4992 5049 5109 6739 7716 1203
Size: 1250 2290 249 57 60 1630 977 1327
Fragments arranged by size:
2290 1630 1327 1250 977 249 60 57
AgeI A'CCGG_T
Cuts at: 6671 6671
Size: 7840
AhdI GACnn_n'nnGTC
Cuts at: 3432 4633 4666 6569 3432
Size: 1201 33 1903 4703
Fragments arranged by size:
4703 1903 1201 33
AleI CACnn'nnGTG
Cuts at: 1166 4736 1166
Size: 3570 4270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3837 1184
Size: 2653 5187
AlwNI CAG_nnn'CTG
Cuts at: 2143 4090 4140 4222 4858 5583 7155 2143
Size: 1947 50 82 636 725 1572 2828
Fragments arranged by size:
2828 1947 1572 725 636 82 50
ApaI G_GGCC'C
Cuts at: 2053 2053
Size: 7840
ApaLI G'TGCA_C
Cuts at: 4739 5897 7053 4739
Size: 1158 1156 5526
Fragments arranged by size:
5526 1158 1156
ApoI r'AATT_y
Cuts at: 1493 2059 2357 2597 2748 2882 3148 3763 3782
Size: 566 298 240 151 134 266 615 19
Cuts at: 3782 4210 4829 5149 5375 5527 7636 1493
Size: 428 619 320 226 152 2109 1697
Fragments arranged by size:
2109 1697 619 615 566 428 320 298
266 240 226 152 151 134 19
AseI AT'TA_AT
Cuts at: 160 1032 6394 160
Size: 872 5362 1606
Fragments arranged by size:
5362 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7840
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 3280 4312 5165 1870
Size: 73 309 1028 1032 853 4545
Fragments arranged by size:
4545 1032 1028 853 309 73
BamHI G'GATC_C
Cuts at: 5170 5537 5170
Size: 367 7473
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2403 3203 3340 4465 4752
Size: 325 217 627 616 800 137 1125 287
Cuts at: 4752 5161 6616 618
Size: 409 1455 1842
Fragments arranged by size:
1842 1455 1125 800 627 616 409 325
287 217 137
BanII G_rGCy'C
Cuts at: 1391 2053 2185 2970 3287 3515 3977 4326 5159
Size: 662 132 785 317 228 462 349 833
Cuts at: 5159 1391
Size: 4072
Fragments arranged by size:
4072 833 785 662 462 349 317 228
132
BbeI G_GCGC'C
Cuts at: 3344 3344
Size: 7840
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4458 928
Size: 971 2559 4310
Fragments arranged by size:
4310 2559 971
BbvCI CC'TCA_GC
Cuts at: 2565 3396 4301 4823 2565
Size: 831 905 522 5582
Fragments arranged by size:
5582 905 831 522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6031 6065 1903
Size: 34 4094 34 3678
Fragments arranged by size:
4094 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2866 5732 6942 2866
Size: 2866 1210 3764
Fragments arranged by size:
3764 2866 1210
BclI T'GATC_A
Cuts at: 1548 2263 3540 4197 1548
Size: 715 1277 657 5191
Fragments arranged by size:
5191 1277 715 657
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3338 3950 4154
Size: 107 122 71 631 605 1666 612 204
Cuts at: 4154 4655 6451 136
Size: 501 1796 1525
Fragments arranged by size:
1796 1666 1525 631 612 605 501 204
122 107 71
BglII A'GATC_T
Cuts at: 7835 7835
Size: 7840
BlpI GC'TnA_GC
Cuts at: 2810 5257 2810
Size: 2447 5393
Bme1580I G_kGCm'C
Cuts at: 1790 2053 4470 4743 5901 7057 1790
Size: 263 2417 273 1158 1156 2573
Fragments arranged by size:
2573 2417 1158 1156 273 263
BmgBI CAC'GTC
Cuts at: 1318 2454 5020 1318
Size: 1136 2566 4138
Fragments arranged by size:
4138 2566 1136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2054 2800 5571
Size: 416 53 83 399 24 637 746 2771
Cuts at: 5571 6529 442
Size: 958 1753
Fragments arranged by size:
2771 1753 958 746 637 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7840
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3411 3411
Size: 7840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3182 3198 3977 4007 4112 4310 4724 6500
Size: 1943 16 779 30 105 198 414 1776
Cuts at: 6500 1239
Size: 2579
Fragments arranged by size:
2579 1943 1776 779 414 198 105 30
16
Bpu10I CC'TnA_GC
Cuts at: 2565 3396 3509 3639 4301 4682 4823 2565
Size: 831 113 130 662 381 141 5582
Fragments arranged by size:
5582 831 662 381 141 130 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2424 3785 4217 5965 6830 7128 2424
Size: 1361 432 1748 865 298 3136
Fragments arranged by size:
3136 1748 1361 865 432 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6503 882
Size: 617 5004 2219
Fragments arranged by size:
5004 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4744 4993 5110 5631 493
Size: 711 3540 249 117 521 2702
Fragments arranged by size:
3540 2702 711 521 249 117
BsaBI GATnn'nnATC
Cuts at: 7834 7834
Size: 7840
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3341 3726 4356 5647
Size: 53 83 186 936 1808 385 630 1291
Cuts at: 5647 6029 6664 275
Size: 382 635 1451
Fragments arranged by size:
1808 1451 1291 936 635 630 385 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2685 3117 6273 6671 6945 7092 7503 2685
Size: 432 3156 398 274 147 411 3022
Fragments arranged by size:
3156 3022 432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3257 3287 3484 3514 4085
Size: 30 165 30 1854 30 197 30 571
Cuts at: 4085 4115 4790 4820 1178
Size: 30 675 30 4198
Fragments arranged by size:
4198 1854 675 571 197 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3310 3371 3374 4457 1186
Size: 2124 61 3 1083 4569
Fragments arranged by size:
4569 2124 1083 61 3
BseYI C'CCAG_C
Cuts at: 3381 7043 7539 3381
Size: 3662 496 3682
Fragments arranged by size:
3682 3662 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3101 4046 4208 4988 1163
Size: 573 1365 945 162 780 4015
Fragments arranged by size:
4015 1365 945 780 573 162
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5290 6051 7079 1540
Size: 275 186 3289 761 1028 2301
Fragments arranged by size:
3289 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2185 2541 3182 3710 3977 4743 5901 5986 7057
Size: 356 641 528 267 766 1158 85 1071
Cuts at: 7057 2185
Size: 2968
Fragments arranged by size:
2968 1158 1071 766 641 528 356 267
85
BsmI GAATG_Cn'
Cuts at: 5351 5444 5351
Size: 93 7747
BsmBI CGTCTCn'nnnn_
Cuts at: 4774 4774
Size: 7840
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2185 2541 2970 3182 3287 3515
Size: 399 263 132 356 429 212 105 228
Cuts at: 3515 3710 3977 4326 4470 4743 5159 5901 5986
Size: 195 267 349 144 273 416 742 85
Cuts at: 5986 7057 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 429 416 399 356 349
273 267 263 228 212 195 144 132
105 85
BspEI T'CCGG_A
Cuts at: 3117 7503 3117
Size: 4386 3454
BspHI T'CATG_A
Cuts at: 5729 7459 5729
Size: 1730 6110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2410 2436 3031 4827 5195 844
Size: 1566 26 595 1796 368 3489
Fragments arranged by size:
3489 1796 1566 595 368 26
BsrBI CCG'CTC
Cuts at: 1394 1823 3840 4036 4096 4423 5727 1394
Size: 429 2017 196 60 327 1304 3507
Fragments arranged by size:
3507 2017 1304 429 327 196 60
BsrDI GCAATG_nn'
Cuts at: 65 1248 6335 6509 65
Size: 1183 5087 174 1396
Fragments arranged by size:
5087 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3231 4065 6484 6671 1700
Size: 110 1421 834 2419 187 2869
Fragments arranged by size:
2869 2419 1421 834 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7840
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7840
BssSI C'ACGA_G
Cuts at: 2120 2228 4369 5900 6912 2120
Size: 108 2141 1531 1012 3048
Fragments arranged by size:
3048 2141 1531 1012 108
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7840
BstBI TT'CG_AA
Cuts at: 5055 6644 5055
Size: 1589 6251
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7840
BstXI CCAn_nnnn'nTGG
Cuts at: 4510 4573 4510
Size: 63 7777
BstYI r'GATC_y
Cuts at: 1972 2290 3676 5170 5537 5922 5939 7391 7477
Size: 318 1386 1494 367 385 17 1452 86
Cuts at: 7477 7489 7835 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1494 1452 1386 385 367 346 318
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3306 3345 4186 4408 4653 4723
Size: 552 939 1302 39 841 222 245 70
Cuts at: 4723 4773 513
Size: 50 3580
Fragments arranged by size:
3580 1302 939 841 552 245 222 70
50 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3891 4683 4774 487
Size: 683 56 2665 792 91 3553
Fragments arranged by size:
3553 2665 792 683 91 56
BtsI GCAGTG_nn'
Cuts at: 4058 4471 4800 5086 5356 6150 6170 4058
Size: 413 329 286 270 794 20 5728
Fragments arranged by size:
5728 794 413 329 286 270 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7840
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7805
DraI TTT'AAA
Cuts at: 4402 5144 4402
Size: 742 7098
DraIII CAC_nnn'GTG
Cuts at: 2283 2494 3696 2283
Size: 211 1202 6427
Fragments arranged by size:
6427 1202 211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6847 809
Size: 507 5531 1802
Fragments arranged by size:
5531 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2697 2730 2846
Size: 54 1425 50 275 18 867 33 116
Cuts at: 2846 2892 4055 4512 4563 4611 5287 6176 7805
Size: 46 1163 457 51 48 676 889 1629
Cuts at: 7805 8
Size: 43
Fragments arranged by size:
1629 1425 1163 889 867 676 457 275
116 54 51 50 48 46 43 33
18
EagI C'GGCC_G
Cuts at: 1537 1812 5287 1537
Size: 275 3475 4090
Fragments arranged by size:
4090 3475 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2958 3348 3599 4577 5770 1353
Size: 1605 390 251 978 1193 3423
Fragments arranged by size:
3423 1605 1193 978 390 251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2355 3247 3338 4592 5051 6427
Size: 1064 138 766 892 91 1254 459 1376
Cuts at: 6427 6801 6947 387
Size: 374 146 1280
Fragments arranged by size:
1376 1280 1254 1064 892 766 459 374
146 138 91
EcoICRI GAG'CTC
Cuts at: 2183 3975 2183
Size: 1792 6048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 3182 3198 3692 3908 3977 4007
Size: 393 462 1088 16 494 216 69 30
Cuts at: 4007 4112 4117 4310 4724 4768 5014 5903 6500
Size: 105 5 193 414 44 246 889 597
Cuts at: 6500 7287 7555 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1088 889 787 597 494 462 414
393 268 246 216 193 105 69 44
30 16 5
EcoO109I rG'GnC_Cy
Cuts at: 2049 2050 3378 3952 4087 4714 2049
Size: 1 1328 574 135 627 5175
Fragments arranged by size:
5175 1328 627 574 135 1
EcoRI G'AATT_C
Cuts at: 2748 2882 3148 5149 5527 2748
Size: 134 266 2001 378 5061
Fragments arranged by size:
5061 2001 378 266 134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2830 5023 5147 2449
Size: 381 2193 124 5142
Fragments arranged by size:
5142 2193 381 124
FspI TGC'GCA
Cuts at: 6346 6346
Size: 7840
HaeII r_GCGC'y
Cuts at: 1678 2830 3344 4689 4711 6987 1678
Size: 1152 514 1345 22 2276 2531
Fragments arranged by size:
2531 2276 1345 1152 514 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2647 2679 2881 2913 3257
Size: 32 135 32 1075 32 202 32 344
Cuts at: 3257 3289 3418 3450 3484 3516 4619 4651 4652
Size: 32 129 32 34 32 1103 32 1
Cuts at: 4652 4684 6481 6513 6555 6587 1373
Size: 32 1797 32 42 32 2626
Fragments arranged by size:
2626 1797 1103 1075 344 202 135 129
42 34 32 32 32 32 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2451 5184 5430 669
Size: 988 281 513 2733 246 3079
Fragments arranged by size:
3079 2733 988 513 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7840
HpaI GTT'AAC
Cuts at: 5430 5430
Size: 7840
KasI G'GCGC_C
Cuts at: 3340 3340
Size: 7840
KpnI G_GTAC'C
Cuts at: 2407 5165 2407
Size: 2758 5082
MfeI C'AATT_G
Cuts at: 5439 5439
Size: 7840
MluI A'CGCG_T
Cuts at: 7716 7716
Size: 7840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3008 6954 7138 1305
Size: 1703 3946 184 2007
Fragments arranged by size:
3946 2007 1703 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2894 4565 4613 10
Size: 54 1425 343 1062 1671 48 3237
Fragments arranged by size:
3237 1671 1425 1062 343 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 4169 4508 4559 4736 4793 5798 6157
Size: 648 3003 339 51 177 57 1005 359
Cuts at: 6157 6316 518
Size: 159 2042
Fragments arranged by size:
3003 2042 1005 648 359 339 177 159
57 51
MspA1I CmG'CkG
Cuts at: 1606 3308 3550 4495 4655 4725 5256 5933 7081
Size: 1702 242 945 160 70 531 677 1148
Cuts at: 7081 7326 1606
Size: 245 2120
Fragments arranged by size:
2120 1702 1148 945 677 531 245 242
160 70
NaeI GCC'GGC
Cuts at: 3233 3233
Size: 7840
NarI GG'CG_CC
Cuts at: 3341 3341
Size: 7840
NcoI C'CATG_G
Cuts at: 513 1065 2004 4773 513
Size: 552 939 2769 3580
Fragments arranged by size:
3580 2769 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7840
NgoMIV G'CCGG_C
Cuts at: 3231 3231
Size: 7840
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7840
NotI GC'GGCC_GC
Cuts at: 5287 5287
Size: 7840
NruI TCG'CGA
Cuts at: 5101 5101
Size: 7840
NspI r_CATG'y
Cuts at: 1132 1229 4339 5198 6743 1132
Size: 97 3110 859 1545 2229
Fragments arranged by size:
3110 2229 1545 859 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7840
PciI A'CATG_T
Cuts at: 6739 6739
Size: 7840
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 4090 1108
Size: 1035 1947 4858
Fragments arranged by size:
4858 1947 1035
PfoI T'CCnGG_A
Cuts at: 4089 4270 4089
Size: 181 7659
PmeI GTTT'AAAC
Cuts at: 5144 5144
Size: 7840
PmlI CAC'GTG
Cuts at: 4744 5110 4744
Size: 366 7474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 5909 5941 7422 7454 5036
Size: 32 841 32 1481 32 5422
Fragments arranged by size:
5422 1481 841 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3378 4087 3378
Size: 709 7131
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7840
PsiI TTA'TAA
Cuts at: 5410 5410
Size: 7840
PspOMI G'GGCC_C
Cuts at: 2049 2049
Size: 7840
PspXI vC'TCGA_Gb
Cuts at: 2252 2252
Size: 7840
PstI C_TGCA'G
Cuts at: 830 2421 4001 4017 5192 830
Size: 1591 1580 16 1175 3478
Fragments arranged by size:
3478 1591 1580 1175 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7840
RsrII CG'GwC_CG
Cuts at: 4527 4527
Size: 7840
SacI G_AGCT'C
Cuts at: 2185 3977 2185
Size: 1792 6048
SacII CC_GC'GG
Cuts at: 3309 4656 4726 3309
Size: 1347 70 6423
Fragments arranged by size:
6423 1347 70
SalI G'TCGA_C
Cuts at: 1936 5182 1936
Size: 3246 4594
SapI GCTCTTCn'nnn_
Cuts at: 2958 4577 2958
Size: 1619 6221
SbfI CC_TGCA'GG
Cuts at: 5192 5192
Size: 7840
ScaI AGT'ACT
Cuts at: 6088 7800 6088
Size: 1712 6128
SexAI A'CCwGG_T
Cuts at: 4083 4083
Size: 7840
SfcI C'TryA_G
Cuts at: 826 1045 2417 3138 3997 4013 5188 6323 7004
Size: 219 1372 721 859 16 1175 1135 681
Cuts at: 7004 7195 826
Size: 191 1471
Fragments arranged by size:
1471 1372 1175 1135 859 721 681 219
191 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 3950 3950
Size: 7840
SfoI GGC'GCC
Cuts at: 3342 3342
Size: 7840
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7840
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7840
SmaI CCC'GGG
Cuts at: 1872 5167 1872
Size: 3295 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 2439 3800 4232 5944 6845
Size: 197 926 309 187 1361 432 1712 901
Cuts at: 6845 7107 820
Size: 262 1553
Fragments arranged by size:
1712 1553 1361 926 901 432 309 262
197 187
SnaBI TAC'GTA
Cuts at: 493 5631 493
Size: 5138 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7840
SphI G_CATG'C
Cuts at: 1132 5198 1132
Size: 4066 3774
SspI AAT'ATT
Cuts at: 5 52 5764 5
Size: 47 5712 2081
Fragments arranged by size:
5712 2081 47
StuI AGG'CCT
Cuts at: 1517 3530 3566 3944 1517
Size: 2013 36 378 5413
Fragments arranged by size:
5413 2013 378 36
StyI C'CwwG_G
Cuts at: 513 1065 2004 2076 2526 2700 2796 2877 3945
Size: 552 939 72 450 174 96 81 1068
Cuts at: 3945 4773 5279 513
Size: 828 506 3074
Fragments arranged by size:
3074 1068 939 828 552 506 450 174
96 81 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 4515 4852 5867 5884 6037 6222
Size: 1255 588 2347 337 1015 17 153 185
Cuts at: 6222 6667 325
Size: 445 1498
Fragments arranged by size:
2347 1498 1255 1015 588 445 337 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5046 6086 7798
Size: 274 80 33 51 1443 3069 1040 1712
Cuts at: 7798 96
Size: 138
Fragments arranged by size:
3069 1712 1443 1040 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2106 2138 2739 2771 2914 2946 4085 4117 4997
Size: 32 601 32 143 32 1139 32 880
Cuts at: 4997 5029 7800 7832 2106
Size: 32 2771 32 2114
Fragments arranged by size:
2771 2114 1139 880 601 143 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3496 1316
Size: 2180 5660
XbaI T'CTAG_A
Cuts at: 5176 5176
Size: 7840
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2612 4771 2612
Size: 2159 5681
XhoI C'TCGA_G
Cuts at: 1943 2252 1943
Size: 309 7531
XmaI C'CCGG_G
Cuts at: 1870 5165 1870
Size: 3295 4545
XmnI GAAnn'nnTTC
Cuts at: 3608 5969 3608
Size: 2361 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4357 5648 276
Size: 53 83 186 936 2823 1291 2468
Fragments arranged by size:
2823 2468 1291 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoNI EcoRV FseI FspAI NsiI PacI PsrI PvuII
SanDI SrfI SwaI