Restriction map for
pFN21AB7009
(Circular) (Six-base) MAPSORT of: pFN21AB7009.seq Check: 4950 from: 1 to: 7921
pFN21AB7009 7921 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3497 3497
Size: 7921
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5731 278
Size: 53 83 186 936 4195 2468
Fragments arranged by size:
4195 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 3954 5264 1937
Size: 909 1108 1310 4594
Fragments arranged by size:
4594 1310 1108 909
Acc65I G'GTAC_C
Cuts at: 2795 5185 5242 2795
Size: 2390 57 5474
Fragments arranged by size:
5474 2390 57
AclI AA'CG_TT
Cuts at: 1563 6048 6421 1563
Size: 4485 373 3063
Fragments arranged by size:
4485 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3182 4580 4814 5984 7368 7636 1632
Size: 1550 1398 234 1170 1384 268 1917
Fragments arranged by size:
1917 1550 1398 1384 1170 268 234
AfeI AGC'GCT
Cuts at: 5099 5099
Size: 7921
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7724
AflIII A'CryG_T
Cuts at: 1203 6820 7797 1203
Size: 5617 977 1327
Fragments arranged by size:
5617 1327 977
AgeI A'CCGG_T
Cuts at: 6752 6752
Size: 7921
AhdI GACnn_n'nnGTC
Cuts at: 6650 6650
Size: 7921
AleI CACnn'nnGTG
Cuts at: 1166 3557 3810 1166
Size: 2391 253 5277
Fragments arranged by size:
5277 2391 253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2457 2940 3203 3225 3575 1184
Size: 1273 483 263 22 350 5530
Fragments arranged by size:
5530 1273 483 350 263 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3001 3033 4373 4405 3001
Size: 32 1340 32 6517
Fragments arranged by size:
6517 1340 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2524 2710 2776 3104 3420 3931 4144 4704 4837
Size: 186 66 328 316 511 213 560 133
Cuts at: 4837 4898 5173 5664 7236 2524
Size: 61 275 491 1572 3209
Fragments arranged by size:
3209 1572 560 511 491 328 316 275
213 186 133 66 61
ApaI G_GGCC'C
Cuts at: 3387 3829 4120 4888 3387
Size: 442 291 768 6420
Fragments arranged by size:
6420 768 442 291
ApaLI G'TGCA_C
Cuts at: 4159 5978 7134 4159
Size: 1819 1156 4946
Fragments arranged by size:
4946 1819 1156
ApoI r'AATT_y
Cuts at: 1493 2576 5230 5456 5608 7717 1493
Size: 1083 2654 226 152 2109 1697
Fragments arranged by size:
2654 2109 1697 1083 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6475 160
Size: 872 5443 1606
Fragments arranged by size:
5443 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
AvaI C'yCGr_G
Cuts at: 1870 1943 2982 3168 3271 3547 4647 4785 4887
Size: 73 1039 186 103 276 1100 138 102
Cuts at: 4887 5246 1870
Size: 359 4545
Fragments arranged by size:
4545 1100 1039 359 276 186 138 103
102 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2275 2308 2275
Size: 33 7888
BamHI G'GATC_C
Cuts at: 5137 5251 5618 5137
Size: 114 367 7440
Fragments arranged by size:
7440 367 114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2795 3509 3559 3644 5185
Size: 325 217 627 1008 714 50 85 1541
Cuts at: 5185 5242 6697 618
Size: 57 1455 1842
Fragments arranged by size:
1842 1541 1455 1008 714 627 325 217
85 57 50
BanII G_rGCy'C
Cuts at: 1391 2199 2484 2810 3278 3387 3753 3829 3861
Size: 808 285 326 468 109 366 76 32
Cuts at: 3861 3897 4120 4888 4947 5240 1391
Size: 36 223 768 59 293 4072
Fragments arranged by size:
4072 808 768 468 366 326 293 285
223 109 76 59 36 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2547 928
Size: 971 648 6302
Fragments arranged by size:
6302 971 648
BbvCI CC'TCA_GC
Cuts at: 2271 2824 2271
Size: 553 7368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2836 2870 4499 4533 4638
Size: 34 50 34 815 34 1629 34 105
Cuts at: 4638 4672 6112 6146 1903
Size: 34 1440 34 3678
Fragments arranged by size:
3678 1629 1440 815 105 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5213 5813 7023 5213
Size: 600 1210 6111
Fragments arranged by size:
6111 1210 600
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7921
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6532 136
Size: 107 122 71 631 605 4860 1525
Fragments arranged by size:
4860 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3525 7916 3525
Size: 4391 3530
BlpI GC'TnA_GC
Cuts at: 2483 3612 4280 5338 2483
Size: 1129 668 1058 5066
Fragments arranged by size:
5066 1129 1058 668
Bme1580I G_kGCm'C
Cuts at: 1790 3387 3406 3554 3829 4120 4163 4238 4405
Size: 1597 19 148 275 291 43 75 167
Cuts at: 4405 4888 5982 7138 1790
Size: 483 1094 1156 2573
Fragments arranged by size:
2573 1597 1156 1094 483 291 275 167
148 75 43 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2805 2836 3408
Size: 416 53 83 399 24 1388 31 572
Cuts at: 3408 4399 4453 4672 5652 6610 442
Size: 991 54 219 980 958 1753
Fragments arranged by size:
1753 1388 991 980 958 572 416 399
219 83 54 53 31 24
BmtI G_CTAG'C
Cuts at: 1056 2138 1056
Size: 1082 6839
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3547 4811 3547
Size: 1264 6657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2261 2546 3051 3455 3620 3737 3773
Size: 878 144 285 505 404 165 117 36
Cuts at: 3773 4463 4860 4920 4959 5102 5151 6581 1239
Size: 690 397 60 39 143 49 1430 2579
Fragments arranged by size:
2579 1430 878 690 505 404 397 285
165 144 143 117 60 49 39 36
Bpu10I CC'TnA_GC
Cuts at: 2271 2804 2824 3891 4946 2271
Size: 533 20 1067 1055 5246
Fragments arranged by size:
5246 1067 1055 533 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6046 6911 7209 6046
Size: 865 298 6758
Fragments arranged by size:
6758 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3789 6584 882
Size: 617 2290 2795 2219
Fragments arranged by size:
2795 2290 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5110 5712 493
Size: 711 3906 602 2702
Fragments arranged by size:
3906 2702 711 602
BsaBI GATnn'nnATC
Cuts at: 7915 7915
Size: 7921
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2160 3394 5728 6110
Size: 53 83 186 936 627 1234 2334 382
Cuts at: 6110 6745 275
Size: 635 1451
Fragments arranged by size:
2334 1451 1234 936 635 627 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3303 4131 6354 6752 7026 7173 7584 3303
Size: 828 2223 398 274 147 411 3640
Fragments arranged by size:
3640 2223 828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2549 3740 3770 3801
Size: 30 165 30 1116 30 1191 30 31
Cuts at: 3801 3831 4858 4888 1178
Size: 30 1027 30 4211
Fragments arranged by size:
4211 1191 1116 1027 165 31 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2063 2083 2396 2435 3536 3728 3791
Size: 850 27 20 313 39 1101 192 63
Cuts at: 3791 4517 4665 1186
Size: 726 148 4442
Fragments arranged by size:
4442 1101 850 726 313 192 148 63
39 27 20
BseYI C'CCAG_C
Cuts at: 3141 3149 3282 3640 3965 3969 4113 4880 5053
Size: 8 133 358 325 4 144 767 173
Cuts at: 5053 5118 7124 7620 3141
Size: 65 2006 496 3442
Fragments arranged by size:
3442 2006 767 496 358 325 173 144
133 65 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 2669 3764 4754 5090 1163
Size: 573 624 309 1095 990 336 3994
Fragments arranged by size:
3994 1095 990 624 573 336 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2111 2254 5371 6132 7160 1540
Size: 275 186 110 143 3117 761 1028 2301
Fragments arranged by size:
3117 2301 1028 761 275 186 143 110
BsiHKAI G_wGCw'C
Cuts at: 2436 2484 2758 2905 2961 3753 4163 4412 5982
Size: 48 274 147 56 792 410 249 1570
Cuts at: 5982 6067 7138 2436
Size: 85 1071 3219
Fragments arranged by size:
3219 1570 1071 792 410 274 249 147
85 56 48
BsiWI C'GTAC_G
Cuts at: 4635 5110 4635
Size: 475 7446
BsmI GAATG_Cn'
Cuts at: 5432 5525 5432
Size: 93 7828
BsmBI CGTCTCn'nnnn_
Cuts at: 5078 5078
Size: 7921
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2436 2484 2758 2810 2905 2961
Size: 399 409 237 48 274 52 95 56
Cuts at: 2961 3278 3387 3406 3554 3753 3829 3861 3897
Size: 317 109 19 148 199 76 32 36
Cuts at: 3897 4120 4163 4238 4405 4412 4888 4947 5240
Size: 223 43 75 167 7 476 59 293
Cuts at: 5240 5982 6067 7138 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 409 399 317 293
274 237 223 199 167 148 109 95
85 76 75 59 56 52 48 43
36 32 19 7
BspEI T'CCGG_A
Cuts at: 7584 7584
Size: 7921
BspHI T'CATG_A
Cuts at: 5810 7540 5810
Size: 1730 6191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 2827 3497 4812 5112 5276 844
Size: 1313 670 670 1315 300 164 3489
Fragments arranged by size:
3489 1315 1313 670 670 300 164
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 5808 1394
Size: 429 203 3782 3507
Fragments arranged by size:
3782 3507 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 4593 6416 6590 65
Size: 1183 3345 1823 174 1396
Fragments arranged by size:
3345 1823 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4988 6565 6752 1700
Size: 110 3178 1577 187 2869
Fragments arranged by size:
3178 2869 1577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7921
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7921
BssSI C'ACGA_G
Cuts at: 5981 6993 5981
Size: 1012 6909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3332 3574 4614 4704 794
Size: 2538 242 1040 90 4011
Fragments arranged by size:
4011 2538 1040 242 90
BstBI TT'CG_AA
Cuts at: 6725 6725
Size: 7921
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7921
BstXI CCAn_nnnn'nTGG
Cuts at: 3029 3157 3358 3029
Size: 128 201 7592
Fragments arranged by size:
7592 201 128
BstYI r'GATC_y
Cuts at: 1972 3427 3525 5137 5251 5618 6003 6020 7472
Size: 1455 98 1612 114 367 385 17 1452
Cuts at: 7472 7558 7570 7916 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1612 1455 1452 385 367 346 114
98 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2066 3774 2066
Size: 1708 6213
BtgI C'CryG_G
Cuts at: 513 1065 2223 2737 2834 513
Size: 552 1158 514 97 5600
Fragments arranged by size:
5600 1158 552 514 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4183 487
Size: 683 56 2957 4225
Fragments arranged by size:
4225 2957 683 56
BtsI GCAGTG_nn'
Cuts at: 2681 2686 2961 3575 3995 5437 6231 6251 2681
Size: 5 275 614 420 1442 794 20 4351
Fragments arranged by size:
4351 1442 794 614 420 275 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7921
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7886
DraI TTT'AAA
Cuts at: 5225 5225
Size: 7921
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4853 6928 809
Size: 507 3537 2075 1802
Fragments arranged by size:
3537 2075 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2108 2251 4246
Size: 54 1425 50 275 18 278 143 1995
Cuts at: 4246 4774 5088 5368 6257 7886 8
Size: 528 314 280 889 1629 43
Fragments arranged by size:
1995 1629 1425 889 528 314 280 278
275 143 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2108 2251 5368 1537
Size: 275 296 143 3117 4090
Fragments arranged by size:
4090 3117 296 275 143
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 4802 5070 5175 5851 1353
Size: 660 2789 268 105 676 3423
Fragments arranged by size:
3423 2789 676 660 268 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2756 3854 4172 5230 6508 6882
Size: 1064 138 1167 1098 318 1058 1278 374
Cuts at: 6882 7028 387
Size: 146 1280
Fragments arranged by size:
1280 1278 1167 1098 1064 1058 374 318
146 138
EcoICRI GAG'CTC
Cuts at: 2482 3751 2482
Size: 1269 6652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2261 2546 3051 3182 3455 3620
Size: 393 485 144 285 505 131 273 165
Cuts at: 3620 3737 3773 4463 4580 4814 4860 4920 4959
Size: 117 36 690 117 234 46 60 39
Cuts at: 4959 5102 5151 5984 6581 7368 7636 1239
Size: 143 49 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 690 597 505 485 393
285 273 268 234 165 144 143 131
117 117 60 49 46 39 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3200 4997 3200
Size: 1797 6124
EcoO109I rG'GnC_Cy
Cuts at: 2707 2785 3017 3368 3384 3631 3826 4117 4141
Size: 78 232 351 16 247 195 291 24
Cuts at: 4141 4381 4579 4884 2707
Size: 240 198 305 5744
Fragments arranged by size:
5744 351 305 291 247 240 232 198
195 78 24 16
EcoRI G'AATT_C
Cuts at: 5230 5608 5230
Size: 378 7543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5036 5036
Size: 7921
FspI TGC'GCA
Cuts at: 6427 6427
Size: 7921
HaeII r_GCGC'y
Cuts at: 1678 2064 3445 3847 3874 4495 5101 7068 1678
Size: 386 1381 402 27 621 606 1967 2531
Fragments arranged by size:
2531 1967 1381 621 606 402 386 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3740 3767 3772 3799 5187
Size: 32 135 32 2168 27 5 27 1388
Cuts at: 5187 5219 6562 6594 6636 6668 1373
Size: 32 1343 32 42 32 2626
Fragments arranged by size:
2626 2168 1388 1343 135 42 32 32
32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 5265 5511 669
Size: 988 281 909 2418 246 3079
Fragments arranged by size:
3079 2418 988 909 281 246
HindIII A'AGCT_T
Cuts at: 748 4956 748
Size: 4208 3713
HpaI GTT'AAC
Cuts at: 5511 5511
Size: 7921
KpnI G_GTAC'C
Cuts at: 2799 5189 5246 2799
Size: 2390 57 5474
Fragments arranged by size:
5474 2390 57
MfeI C'AATT_G
Cuts at: 5520 5520
Size: 7921
MluI A'CGCG_T
Cuts at: 7797 7797
Size: 7921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2517 4499 7035 7219 1305
Size: 1212 1982 2536 184 2007
Fragments arranged by size:
2536 2007 1982 1212 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6099
Fragments arranged by size:
6099 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3557 3810 4226 5879 6238 6397 518
Size: 648 2391 253 416 1653 359 159 2042
Fragments arranged by size:
2391 2042 1653 648 416 359 253 159
MspA1I CmG'CkG
Cuts at: 1606 2155 2178 2275 2533 3101 3969 4441 4701
Size: 549 23 97 258 568 868 472 260
Cuts at: 4701 4880 4979 5337 6014 7162 7407 1606
Size: 179 99 358 677 1148 245 2120
Fragments arranged by size:
2120 1148 868 677 568 549 472 358
260 258 245 179 99 97 23
NaeI GCC'GGC
Cuts at: 4990 4990
Size: 7921
NcoI C'CATG_G
Cuts at: 513 1065 2737 513
Size: 552 1672 5697
Fragments arranged by size:
5697 1672 552
NdeI CA'TA_TG
Cuts at: 387 3119 387
Size: 2732 5189
NgoMIV G'CCGG_C
Cuts at: 4988 4988
Size: 7921
NheI G'CTAG_C
Cuts at: 1052 2134 1052
Size: 1082 6839
NotI GC'GGCC_GC
Cuts at: 5368 5368
Size: 7921
NruI TCG'CGA
Cuts at: 4314 4314
Size: 7921
NspI r_CATG'y
Cuts at: 1132 1229 3335 4663 5279 6824 1132
Size: 97 2106 1328 616 1545 2229
Fragments arranged by size:
2229 2106 1545 1328 616 97
PasI CC'CwG_GG
Cuts at: 1348 2863 3635 4576 1348
Size: 1515 772 941 4693
Fragments arranged by size:
4693 1515 941 772
PciI A'CATG_T
Cuts at: 6820 6820
Size: 7921
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5047 5125 1108
Size: 3939 78 3904
Fragments arranged by size:
3939 3904 78
PfoI T'CCnGG_A
Cuts at: 2238 3489 3597 2238
Size: 1251 108 6562
Fragments arranged by size:
6562 1251 108
PmeI GTTT'AAAC
Cuts at: 5225 5225
Size: 7921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5990 6022 7503 7535 5990
Size: 32 1481 32 6376
Fragments arranged by size:
6376 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 3017 3368 3631 4381 2785
Size: 232 351 263 750 6325
Fragments arranged by size:
6325 750 351 263 232
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7921
PsiI TTA'TAA
Cuts at: 5491 5491
Size: 7921
PspOMI G'GGCC_C
Cuts at: 3383 3825 4116 4884 3383
Size: 442 291 768 6420
Fragments arranged by size:
6420 768 442 291
PspXI vC'TCGA_Gb
Cuts at: 3271 3271
Size: 7921
PstI C_TGCA'G
Cuts at: 830 2154 2568 2793 3543 3570 4005 4700 4754
Size: 1324 414 225 750 27 435 695 54
Cuts at: 4754 5273 830
Size: 519 3478
Fragments arranged by size:
3478 1324 750 695 519 435 414 225
54 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7921
PvuII CAG'CTG
Cuts at: 2155 2533 3101 3969 4441 4880 4979 2155
Size: 378 568 868 472 439 99 5097
Fragments arranged by size:
5097 868 568 472 439 378 99
SacI G_AGCT'C
Cuts at: 2484 3753 2484
Size: 1269 6652
SalI G'TCGA_C
Cuts at: 1936 2845 5263 1936
Size: 909 2418 4594
Fragments arranged by size:
4594 2418 909
SanDI GG'GwC_CC
Cuts at: 3017 3368 4381 3017
Size: 351 1013 6557
Fragments arranged by size:
6557 1013 351
SapI GCTCTTCn'nnn_
Cuts at: 5070 5070
Size: 7921
SbfI CC_TGCA'GG
Cuts at: 2793 5273 2793
Size: 2480 5441
ScaI AGT'ACT
Cuts at: 2463 3545 6169 7881 2463
Size: 1082 2624 1712 2503
Fragments arranged by size:
2624 2503 1712 1082
SexAI A'CCwGG_T
Cuts at: 2436 2436
Size: 7921
SfcI C'TryA_G
Cuts at: 826 1045 2150 2445 2564 2789 3539 3566 3839
Size: 219 1105 295 119 225 750 27 273
Cuts at: 3839 4001 4696 4750 4841 5269 6404 7085 7276
Size: 162 695 54 91 428 1135 681 191
Cuts at: 7276 826
Size: 1471
Fragments arranged by size:
1471 1135 1105 750 695 681 428 295
273 225 219 191 162 119 91 54
27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7921
SmaI CCC'GGG
Cuts at: 1872 4889 5248 1872
Size: 3017 359 4545
Fragments arranged by size:
4545 3017 359
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3271 4785 6025 6926 7188 820
Size: 197 926 1328 1514 1240 901 262 1553
Fragments arranged by size:
1553 1514 1328 1240 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5712 493
Size: 5219 2702
SpeI A'CTAG_T
Cuts at: 152 3308 152
Size: 3156 4765
SphI G_CATG'C
Cuts at: 1132 4663 5279 1132
Size: 3531 616 3774
Fragments arranged by size:
3774 3531 616
SrfI GCCC'GGGC
Cuts at: 4889 4889
Size: 7921
SspI AAT'ATT
Cuts at: 5 52 5845 5
Size: 47 5793 2081
Fragments arranged by size:
5793 2081 47
StuI AGG'CCT
Cuts at: 1517 3036 3468 1517
Size: 1519 432 5970
Fragments arranged by size:
5970 1519 432
StyI C'CwwG_G
Cuts at: 513 1065 2737 2948 3022 5190 5360 513
Size: 552 1672 211 74 2168 170 3074
Fragments arranged by size:
3074 2168 1672 552 211 170 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2760 3274 3632 4027 4376 5948 5965
Size: 1255 1180 514 358 395 349 1572 17
Cuts at: 5965 6118 6303 6748 325
Size: 153 185 445 1498
Fragments arranged by size:
1572 1498 1255 1180 514 445 395 358
349 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 3543 6167
Size: 274 80 33 51 1443 484 1082 2624
Cuts at: 6167 7879 96
Size: 1712 138
Fragments arranged by size:
2624 1712 1443 1082 484 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2157 2189 2613 2632 2645 2664 2678 2710 3801
Size: 32 424 19 13 19 14 32 1091
Cuts at: 3801 3833 4153 4185 7881 7913 2157
Size: 32 320 32 3696 32 2165
Fragments arranged by size:
3696 2165 1091 424 320 32 32 32
32 32 19 19 14 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7921
XbaI T'CTAG_A
Cuts at: 5257 5257
Size: 7921
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2735 2777 3157 4412 5126 2735
Size: 42 380 1255 714 5530
Fragments arranged by size:
5530 1255 714 380 42
XhoI C'TCGA_G
Cuts at: 1943 3271 4785 1943
Size: 1328 1514 5079
Fragments arranged by size:
5079 1514 1328
XmaI C'CCGG_G
Cuts at: 1870 4887 5246 1870
Size: 3017 359 4545
Fragments arranged by size:
4545 3017 359
XmnI GAAnn'nnTTC
Cuts at: 6050 6050
Size: 7921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5729 276
Size: 53 83 186 936 4195 2468
Fragments arranged by size:
4195 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BfrBI BstZ17I DraIII EcoRV FseI
FspAI KasI NarI NsiI PacI PmlI PsrI RsrII
SacII SfiI SfoI SwaI