Restriction map for
pFN21AB7007
(Circular) (Six-base) MAPSORT of: pFN21AB7007.seq Check: 5983 from: 1 to: 8977
pFN21AB7007 8977 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2163 2927 4011 4345 4660 4675 5295 2163
Size: 764 1084 334 315 15 620 5845
Fragments arranged by size:
5845 1084 764 620 334 315 15
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6787 278
Size: 53 83 186 936 5251 2468
Fragments arranged by size:
5251 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3033 4863 5631 5865 6320 1937
Size: 1096 1830 768 234 455 4594
Fragments arranged by size:
4594 1830 1096 768 455 234
Acc65I G'GTAC_C
Cuts at: 2955 6298 2955
Size: 3343 5634
AclI AA'CG_TT
Cuts at: 1563 7104 7477 1563
Size: 5541 373 3063
Fragments arranged by size:
5541 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2843 3641 4512 5360 5369 5553 7040
Size: 678 533 798 871 848 9 184 1487
Cuts at: 7040 8424 8692 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1487 1384 871 848 798 678 533
268 184 9
AfeI AGC'GCT
Cuts at: 4629 4629
Size: 8977
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8780
AflIII A'CryG_T
Cuts at: 1203 3177 3272 3976 4539 5201 7876 8853 1203
Size: 1974 95 704 563 662 2675 977 1327
Fragments arranged by size:
2675 1974 1327 977 704 662 563 95
AgeI A'CCGG_T
Cuts at: 7808 7808
Size: 8977
AhdI GACnn_n'nnGTC
Cuts at: 5872 5959 7706 5872
Size: 87 1747 7143
Fragments arranged by size:
7143 1747 87
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2162 2359 4259 6038 1184
Size: 978 197 1900 1779 4123
Fragments arranged by size:
4123 1900 1779 978 197
AlwNI CAG_nnn'CTG
Cuts at: 2320 2404 2494 2572 3143 3671 4079 5486 5749
Size: 84 90 78 571 528 408 1407 263
Cuts at: 5749 6720 8292 2320
Size: 971 1572 3005
Fragments arranged by size:
3005 1572 1407 971 571 528 408 263
90 84 78
ApaI G_GGCC'C
Cuts at: 2675 3721 5278 5839 2675
Size: 1046 1557 561 5813
Fragments arranged by size:
5813 1557 1046 561
ApaLI G'TGCA_C
Cuts at: 2310 7034 8190 2310
Size: 4724 1156 3097
Fragments arranged by size:
4724 3097 1156
ApoI r'AATT_y
Cuts at: 1493 2370 3515 4181 4988 5363 5464 5648 6286
Size: 877 1145 666 807 375 101 184 638
Cuts at: 6286 6512 6664 8773 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1145 877 807 666 638 375
226 184 152 101
AseI AT'TA_AT
Cuts at: 160 1032 7531 160
Size: 872 6499 1606
Fragments arranged by size:
6499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8977
AvaI C'yCGr_G
Cuts at: 1870 1943 2586 3394 4591 5277 5832 6302 1870
Size: 73 643 808 1197 686 555 470 4545
Fragments arranged by size:
4545 1197 808 686 643 555 470 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2947 2980 3543 3576 2947
Size: 33 563 33 8348
Fragments arranged by size:
8348 563 33 33
BamHI G'GATC_C
Cuts at: 3337 6307 6674 3337
Size: 2970 367 5640
Fragments arranged by size:
5640 2970 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2497 2653 2720 2955 3466
Size: 325 217 627 710 156 67 235 511
Cuts at: 3466 4842 6298 7753 618
Size: 1376 1456 1455 1842
Fragments arranged by size:
1842 1456 1455 1376 710 627 511 325
235 217 156 67
BanII G_rGCy'C
Cuts at: 1391 2526 2675 3371 3711 3721 3755 3898 3926
Size: 1135 149 696 340 10 34 143 28
Cuts at: 3926 5278 5589 5802 5839 5985 6296 1391
Size: 1352 311 213 37 146 311 4072
Fragments arranged by size:
4072 1352 1135 696 340 311 311 213
149 146 143 37 34 28 10
BbeI G_GCGC'C
Cuts at: 2724 2724
Size: 8977
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2039 3084 928
Size: 971 140 1045 6821
Fragments arranged by size:
6821 1045 971 140
BbvCI CC'TCA_GC
Cuts at: 2397 5750 6039 2397
Size: 3353 289 5335
Fragments arranged by size:
5335 3353 289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7168 7202 1903
Size: 34 5231 34 3678
Fragments arranged by size:
5231 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6869 8079 6869
Size: 1210 7767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8977
BfrBI ATG'CAT
Cuts at: 4118 4118
Size: 8977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2670 4608 7588
Size: 107 122 71 631 605 998 1938 2980
Cuts at: 7588 136
Size: 1525
Fragments arranged by size:
2980 1938 1525 998 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5109 8972 5109
Size: 3863 5114
BlpI GC'TnA_GC
Cuts at: 3194 6394 3194
Size: 3200 5777
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2314 2675 3721 4845 5001 5278 5839
Size: 384 140 361 1046 1124 156 277 561
Cuts at: 5839 7038 8194 1790
Size: 1199 1156 2573
Fragments arranged by size:
2573 1199 1156 1124 1046 561 384 361
277 156 140
BmgBI CAC'GTC
Cuts at: 1318 2802 4440 4452 4540 1318
Size: 1484 1638 12 88 5755
Fragments arranged by size:
5755 1638 1484 88 12
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3091 3369 6708
Size: 416 53 83 399 24 1674 278 3339
Cuts at: 6708 7666 442
Size: 958 1753
Fragments arranged by size:
3339 1753 1674 958 416 399 278 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2319 4942 5710 6004 2319
Size: 2623 768 294 5292
Fragments arranged by size:
5292 2623 768 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 3023 3391 4146 4912 4995 5261 5279
Size: 1382 402 368 755 766 83 266 18
Cuts at: 5279 7637 1239
Size: 2358 2579
Fragments arranged by size:
2579 2358 1382 766 755 402 368 266
83 18
Bpu10I CC'TnA_GC
Cuts at: 2397 5750 5856 6039 2397
Size: 3353 106 183 5335
Fragments arranged by size:
5335 3353 183 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 3940 7102 7967 8265 2729
Size: 1211 3162 865 298 3441
Fragments arranged by size:
3441 3162 1211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4513 7640 882
Size: 617 3014 3127 2219
Fragments arranged by size:
3127 3014 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3273 6768 493
Size: 711 2069 3495 2702
Fragments arranged by size:
3495 2702 2069 711
BsaBI GATnn'nnATC
Cuts at: 3528 8971 3528
Size: 5443 3534
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2721 3024 6784 7166
Size: 53 83 186 936 1188 303 3760 382
Cuts at: 7166 7801 275
Size: 635 1451
Fragments arranged by size:
3760 1451 1188 936 635 382 303 186
83 53
BsaWI w'CCGG_w
Cuts at: 4300 7410 7808 8082 8229 8640 4300
Size: 3110 398 274 147 411 4637
Fragments arranged by size:
4637 3110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3220 3250 4714 4744 4949
Size: 30 165 30 1817 30 1464 30 205
Cuts at: 4949 4979 6072 6102 1178
Size: 30 1093 30 4053
Fragments arranged by size:
4053 1817 1464 1093 205 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 2245 2726 2984 2987 3008 3379 3624
Size: 1030 29 481 258 3 21 371 245
Cuts at: 3624 3779 5099 5258 5315 5333 5336 5339 5357
Size: 155 1320 159 57 18 3 3 18
Cuts at: 5357 5645 5800 5912 1186
Size: 288 155 112 4251
Fragments arranged by size:
4251 1320 1030 481 371 288 258 245
159 155 155 112 57 29 21 18
18 3 3 3
BseYI C'CCAG_C
Cuts at: 2033 2271 3739 4582 4624 8180 8676 2033
Size: 238 1468 843 42 3556 496 2334
Fragments arranged by size:
3556 2334 1468 843 496 238 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2074 2135 3930 3935 4450 4502 4752
Size: 573 338 61 1795 5 515 52 250
Cuts at: 4752 4917 5001 5226 5273 1163
Size: 165 84 225 47 4867
Fragments arranged by size:
4867 1795 573 515 338 250 225 165
84 61 52 47 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6427 7188 8216 1540
Size: 275 186 4426 761 1028 2301
Fragments arranged by size:
4426 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2314 2526 4923 5286 7038 7123 8194 2314
Size: 212 2397 363 1752 85 1071 3097
Fragments arranged by size:
3097 2397 1752 1071 363 212 85
BsmI GAATG_Cn'
Cuts at: 4372 6488 6581 4372
Size: 2116 93 6768
Fragments arranged by size:
6768 2116 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2314 2526 2675 3371 3711 3721
Size: 399 384 140 212 149 696 340 10
Cuts at: 3721 3755 3898 3926 4845 4923 5001 5278 5286
Size: 34 143 28 919 78 78 277 8
Cuts at: 5286 5589 5802 5839 5985 6296 7038 7123 8194
Size: 303 213 37 146 311 742 85 1071
Cuts at: 8194 1391
Size: 2174
Fragments arranged by size:
2174 1071 919 742 696 399 384 340
311 303 277 213 212 149 146 143
140 85 78 78 37 34 28 10
8
BspEI T'CCGG_A
Cuts at: 8640 8640
Size: 8977
BspHI T'CATG_A
Cuts at: 6866 8596 6866
Size: 1730 7247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2927 4011 4345 4660 4675 5295 6332
Size: 1319 764 1084 334 315 15 620 1037
Cuts at: 6332 844
Size: 3489
Fragments arranged by size:
3489 1319 1084 1037 764 620 334 315
15
BsrBI CCG'CTC
Cuts at: 1394 1823 6864 1394
Size: 429 5041 3507
Fragments arranged by size:
5041 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3888 4333 7472 7646 65
Size: 1183 2640 445 3139 174 1396
Fragments arranged by size:
3139 2640 1396 1183 445 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2282 7621 7808 1700
Size: 110 472 5339 187 2869
Fragments arranged by size:
5339 2869 472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8977
BssSI C'ACGA_G
Cuts at: 2341 2525 7037 8049 2341
Size: 184 4512 1012 3269
Fragments arranged by size:
4512 3269 1012 184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4694 4968 794
Size: 3900 274 4803
Fragments arranged by size:
4803 3900 274
BstBI TT'CG_AA
Cuts at: 7781 7781
Size: 8977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8977
BstXI CCAn_nnnn'nTGG
Cuts at: 4612 4612
Size: 8977
BstYI r'GATC_y
Cuts at: 1972 3337 5109 6307 6674 7059 7076 8528 8614
Size: 1365 1772 1198 367 385 17 1452 86
Cuts at: 8614 8626 8972 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1772 1452 1365 1198 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2124 2190 3970 4605 513
Size: 552 1059 66 1780 635 4885
Fragments arranged by size:
4885 1780 1059 635 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3258 487
Size: 683 56 2032 6206
Fragments arranged by size:
6206 2032 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 2362 2929 3947 4828 6493 7287 7307 2293
Size: 69 567 1018 881 1665 794 20 3963
Fragments arranged by size:
3963 1665 1018 881 794 567 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2783 2818 529
Size: 35 2219 35 6688
Fragments arranged by size:
6688 2219 35 35
DraI TTT'AAA
Cuts at: 3094 6281 3094
Size: 3187 5790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3029 3293 7984 809
Size: 507 1713 264 4691 1802
Fragments arranged by size:
4691 1802 1713 507 264
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2640 3564 3907
Size: 54 1425 50 275 18 810 924 343
Cuts at: 3907 4434 4782 6424 7313 8942 8
Size: 527 348 1642 889 1629 43
Fragments arranged by size:
1642 1629 1425 924 889 810 527 348
343 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6424 1537
Size: 275 4612 4090
Fragments arranged by size:
4612 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3008 4128 5334 5554 6907 1353
Size: 1655 1120 1206 220 1353 3423
Fragments arranged by size:
3423 1655 1353 1206 1120 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2399 2603 7564 7938 8084 387
Size: 1064 138 810 204 4961 374 146 1280
Fragments arranged by size:
4961 1280 1064 810 374 204 146 138
EcoICRI GAG'CTC
Cuts at: 2524 2524
Size: 8977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2621 2843 3023 3391 3641 4146
Size: 393 678 311 222 180 368 250 505
Cuts at: 4146 4512 4912 4995 5261 5279 5360 5369 5553
Size: 366 400 83 266 18 81 9 184
Cuts at: 5553 7040 7637 8424 8692 1239
Size: 1487 597 787 268 1524
Fragments arranged by size:
1524 1487 787 678 597 505 400 393
368 366 311 268 266 250 222 184
180 83 81 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 2729 4530 5297 5660 2708
Size: 21 1801 767 363 6025
Fragments arranged by size:
6025 1801 767 363 21
EcoO109I rG'GnC_Cy
Cuts at: 2548 2671 3717 3718 5274 2548
Size: 123 1046 1 1556 6251
Fragments arranged by size:
6251 1556 1046 123 1
EcoRI G'AATT_C
Cuts at: 4988 6286 6664 4988
Size: 1298 378 7301
Fragments arranged by size:
7301 1298 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2261 3255 5077 2261
Size: 994 1822 6161
Fragments arranged by size:
6161 1822 994
FspI TGC'GCA
Cuts at: 2981 7483 2981
Size: 4502 4475
HaeII r_GCGC'y
Cuts at: 1678 2724 4631 4806 8124 1678
Size: 1046 1907 175 3318 2531
Fragments arranged by size:
3318 2531 1907 1046 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3446 3478 4747 4779 4898
Size: 32 135 32 1874 32 1269 32 119
Cuts at: 4898 4930 5096 5128 5807 5839 5858 5890 5945
Size: 32 166 32 679 32 19 32 55
Cuts at: 5945 5977 6072 6104 6213 6245 7618 7650 7692
Size: 32 95 32 109 32 1373 32 42
Cuts at: 7692 7724 1373
Size: 32 2626
Fragments arranged by size:
2626 1874 1373 1269 679 166 135 119
109 95 55 42 32 32 32 32
32 32 32 32 32 32 32 32
32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 3034 3072 3123 5134 5632 5866
Size: 988 281 1096 38 51 2011 498 234
Cuts at: 5866 5911 6091 6321 6567 669
Size: 45 180 230 246 3079
Fragments arranged by size:
3079 2011 1096 988 498 281 246 234
230 180 51 45 38
HindIII A'AGCT_T
Cuts at: 748 3858 5054 748
Size: 3110 1196 4671
Fragments arranged by size:
4671 3110 1196
HpaI GTT'AAC
Cuts at: 3072 3123 6567 3072
Size: 51 3444 5482
Fragments arranged by size:
5482 3444 51
KasI G'GCGC_C
Cuts at: 2720 2720
Size: 8977
KpnI G_GTAC'C
Cuts at: 2959 6302 2959
Size: 3343 5634
MfeI C'AATT_G
Cuts at: 6099 6576 6099
Size: 477 8500
MluI A'CGCG_T
Cuts at: 5201 8853 5201
Size: 3652 5325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5427 8091 8275 1305
Size: 4122 2664 184 2007
Fragments arranged by size:
4122 2664 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3566 3909 4436 10
Size: 54 1425 343 1734 343 527 4551
Fragments arranged by size:
4551 1734 1425 527 343 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2341 3969 3975 6935 7294 7453 518
Size: 648 1175 1628 6 2960 359 159 2042
Fragments arranged by size:
2960 2042 1628 1175 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2404 2470 2560 2629 4848 4911 5015 5371
Size: 798 66 90 69 2219 63 104 356
Cuts at: 5371 5938 6393 7070 8218 8463 1606
Size: 567 455 677 1148 245 2120
Fragments arranged by size:
2219 2120 1148 798 677 567 455 356
245 104 90 69 66 63
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 8977
NarI GG'CG_CC
Cuts at: 2721 2721
Size: 8977
NcoI C'CATG_G
Cuts at: 513 1065 2124 3970 513
Size: 552 1059 1846 5520
Fragments arranged by size:
5520 1846 1059 552
NdeI CA'TA_TG
Cuts at: 387 5571 387
Size: 5184 3793
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 8977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8977
NotI GC'GGCC_GC
Cuts at: 6424 6424
Size: 8977
NsiI A_TGCA'T
Cuts at: 4120 4120
Size: 8977
NspI r_CATG'y
Cuts at: 1132 1229 2340 3070 3181 3202 3980 5455 6335
Size: 97 1111 730 111 21 778 1475 880
Cuts at: 6335 7880 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1475 1111 880 778 730 111
97 21
PasI CC'CwG_GG
Cuts at: 1348 3713 1348
Size: 2365 6612
PciI A'CATG_T
Cuts at: 3177 3976 7876 3177
Size: 799 3900 4278
Fragments arranged by size:
4278 3900 799
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2494 2572 1108
Size: 1386 78 7513
Fragments arranged by size:
7513 1386 78
PfoI T'CCnGG_A
Cuts at: 5295 5295
Size: 8977
PmeI GTTT'AAAC
Cuts at: 6281 6281
Size: 8977
PmlI CAC'GTG
Cuts at: 3273 3273
Size: 8977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7046 7078 8559 8591 7046
Size: 32 1481 32 7432
Fragments arranged by size:
7432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 2548
Size: 8977
PshAI GACnn'nnGTC
Cuts at: 1613 3252 1613
Size: 1639 7338
PsiI TTA'TAA
Cuts at: 4187 5168 6547 4187
Size: 981 1379 6617
Fragments arranged by size:
6617 1379 981
PspOMI G'GGCC_C
Cuts at: 2671 3717 5274 5835 2671
Size: 1046 1557 561 5813
Fragments arranged by size:
5813 1557 1046 561
PstI C_TGCA'G
Cuts at: 830 2357 2409 2670 2924 2952 3254 4342 4823
Size: 1527 52 261 254 28 302 1088 481
Cuts at: 4823 5623 6329 830
Size: 800 706 3478
Fragments arranged by size:
3478 1527 1088 800 706 481 302 261
254 52 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8977
PvuII CAG'CTG
Cuts at: 2404 2560 2629 4911 5015 5938 2404
Size: 156 69 2282 104 923 5443
Fragments arranged by size:
5443 2282 923 156 104 69
RsrII CG'GwC_CG
Cuts at: 2386 2386
Size: 8977
SacI G_AGCT'C
Cuts at: 2526 2526
Size: 8977
SalI G'TCGA_C
Cuts at: 1936 3032 5630 5864 6319 1936
Size: 1096 2598 234 455 4594
Fragments arranged by size:
4594 2598 1096 455 234
SanDI GG'GwC_CC
Cuts at: 2548 2548
Size: 8977
SbfI CC_TGCA'GG
Cuts at: 2670 6329 2670
Size: 3659 5318
ScaI AGT'ACT
Cuts at: 4967 7225 8937 4967
Size: 2258 1712 5007
Fragments arranged by size:
5007 2258 1712
SfcI C'TryA_G
Cuts at: 826 1045 2353 2405 2666 2920 2948 3223 3250
Size: 219 1308 52 261 254 28 275 27
Cuts at: 3250 4338 4819 5619 6325 7460 8141 8332 826
Size: 1088 481 800 706 1135 681 191 1471
Fragments arranged by size:
1471 1308 1135 1088 800 706 681 481
275 261 254 219 191 52 28 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 2670 2670
Size: 8977
SfoI GGC'GCC
Cuts at: 2722 2722
Size: 8977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8977
SmaI CCC'GGG
Cuts at: 1872 5279 5834 6304 1872
Size: 3407 555 470 4545
Fragments arranged by size:
4545 3407 555 470
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2586 2708 3919 7081 7982 8244
Size: 197 926 643 122 1211 3162 901 262
Cuts at: 8244 820
Size: 1553
Fragments arranged by size:
3162 1553 1211 926 901 643 262 197
122
SnaBI TAC'GTA
Cuts at: 493 6768 493
Size: 6275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8977
SphI G_CATG'C
Cuts at: 1132 2340 3202 6335 1132
Size: 1208 862 3133 3774
Fragments arranged by size:
3774 3133 1208 862
SspI AAT'ATT
Cuts at: 5 52 4272 6176 6901 5
Size: 47 4220 1904 725 2081
Fragments arranged by size:
4220 2081 1904 725 47
StuI AGG'CCT
Cuts at: 1517 2351 1517
Size: 834 8143
StyI C'CwwG_G
Cuts at: 513 1065 2124 2133 2643 2758 3970 5661 5805
Size: 552 1059 9 510 115 1212 1691 144
Cuts at: 5805 6416 513
Size: 611 3074
Fragments arranged by size:
3074 1691 1212 1059 611 552 510 144
115 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2155 2297 7004 7021 7174 7359 7804
Size: 1255 575 142 4707 17 153 185 445
Cuts at: 7804 325
Size: 1498
Fragments arranged by size:
4707 1498 1255 575 445 185 153 142
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4965 7223 8935
Size: 274 80 33 51 1443 2988 2258 1712
Cuts at: 8935 96
Size: 138
Fragments arranged by size:
2988 2258 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3220 3252 4191 4223 5546 5578 8937 8969 3220
Size: 32 939 32 1323 32 3359 32 3228
Fragments arranged by size:
3359 3228 1323 939 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5627 5845 6086 1316
Size: 4311 218 241 4207
Fragments arranged by size:
4311 4207 241 218
XbaI T'CTAG_A
Cuts at: 6313 6313
Size: 8977
XhoI C'TCGA_G
Cuts at: 1943 2586 1943
Size: 643 8334
XmaI C'CCGG_G
Cuts at: 1870 5277 5832 6302 1870
Size: 3407 555 470 4545
Fragments arranged by size:
4545 3407 555 470
XmnI GAAnn'nnTTC
Cuts at: 2789 3155 4308 5055 7106 2789
Size: 366 1153 747 2051 4660
Fragments arranged by size:
4660 2051 1153 747 366
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6785 276
Size: 53 83 186 936 5251 2468
Fragments arranged by size:
5251 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI NruI PacI PspXI PsrI SacII
SapI SexAI SrfI SwaI XcmI