Restriction map for
pFN21AB7006
(Circular) (Six-base) MAPSORT of: pFN21AB7006.seq Check: 3963 from: 1 to: 7903
pFN21AB7006 7903 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5713 278
Size: 53 83 186 936 4177 2468
Fragments arranged by size:
4177 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5246 1937
Size: 3309 4594
Acc65I G'GTAC_C
Cuts at: 4829 5196 5224 4829
Size: 367 28 7508
Fragments arranged by size:
7508 367 28
AclI AA'CG_TT
Cuts at: 1563 6030 6403 1563
Size: 4467 373 3063
Fragments arranged by size:
4467 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2814 3264 4164 4623 5148 5966 7350
Size: 641 541 450 900 459 525 818 1384
Cuts at: 7350 7618 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 900 818 641 541 525 459
450 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7706
AflIII A'CryG_T
Cuts at: 1203 6802 7779 1203
Size: 5599 977 1327
Fragments arranged by size:
5599 1327 977
AgeI A'CCGG_T
Cuts at: 3043 6734 3043
Size: 3691 4212
AhdI GACnn_n'nnGTC
Cuts at: 6632 6632
Size: 7903
AleI CACnn'nnGTG
Cuts at: 1166 3508 1166
Size: 2342 5561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3171 4802 1184
Size: 1987 1631 4285
Fragments arranged by size:
4285 1987 1631
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4322 4354 4640 4672 4322
Size: 32 286 32 7553
Fragments arranged by size:
7553 286 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2312 3082 3421 4249 5159 5646 7218 2312
Size: 770 339 828 910 487 1572 2997
Fragments arranged by size:
2997 1572 910 828 770 487 339
ApaLI G'TGCA_C
Cuts at: 4918 5960 7116 4918
Size: 1042 1156 5705
Fragments arranged by size:
5705 1156 1042
ApoI r'AATT_y
Cuts at: 1493 2424 4321 5145 5212 5438 5590 7699 1493
Size: 931 1897 824 67 226 152 2109 1697
Fragments arranged by size:
2109 1897 1697 931 824 226 152 67
AseI AT'TA_AT
Cuts at: 160 1032 2727 6457 160
Size: 872 1695 3730 1606
Fragments arranged by size:
3730 1695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7903
AvaI C'yCGr_G
Cuts at: 1870 1943 2316 3381 4008 5228 1870
Size: 73 373 1065 627 1220 4545
Fragments arranged by size:
4545 1220 1065 627 373 73
AvrII C'CTAG_G
Cuts at: 4622 4622
Size: 7903
BamHI G'GATC_C
Cuts at: 3035 4290 4330 4473 5233 5600 3035
Size: 1255 40 143 760 367 5338
Fragments arranged by size:
5338 1255 760 367 143 40
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3847 4829 5196 5224 6679
Size: 325 217 627 2060 982 367 28 1455
Cuts at: 6679 618
Size: 1842
Fragments arranged by size:
2060 1842 1455 982 627 367 325 217
28
BanII G_rGCy'C
Cuts at: 1391 2550 5222 1391
Size: 1159 2672 4072
Fragments arranged by size:
4072 2672 1159
BbeI G_GCGC'C
Cuts at: 3851 3851
Size: 7903
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3358 4007 928
Size: 971 1459 649 4824
Fragments arranged by size:
4824 1459 971 649
BbvCI CC'TCA_GC
Cuts at: 2391 2391
Size: 7903
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6094 6128 1903
Size: 34 50 34 4073 34 3678
Fragments arranged by size:
4073 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5795 7005 5795
Size: 1210 6693
BclI T'GATC_A
Cuts at: 1548 3001 1548
Size: 1453 6450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 3616 3668
Size: 107 122 71 631 605 475 1469 52
Cuts at: 3668 3941 6514 136
Size: 273 2573 1525
Fragments arranged by size:
2573 1525 1469 631 605 475 273 122
107 71 52
BglII A'GATC_T
Cuts at: 2061 2907 3343 3622 7898 2061
Size: 846 436 279 4276 2066
Fragments arranged by size:
4276 2066 846 436 279
BlpI GC'TnA_GC
Cuts at: 3063 5320 3063
Size: 2257 5646
Bme1580I G_kGCm'C
Cuts at: 1790 4843 4922 5964 7120 1790
Size: 3053 79 1042 1156 2573
Fragments arranged by size:
3053 2573 1156 1042 79
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7903
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5634 6592 442
Size: 416 53 83 399 24 4217 958 1753
Fragments arranged by size:
4217 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 3357 6563 1239
Size: 824 1294 3206 2579
Fragments arranged by size:
3206 2579 1294 824
Bpu10I CC'TnA_GC
Cuts at: 2391 4662 2391
Size: 2271 5632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3503 6028 6893 7191 3503
Size: 2525 865 298 4215
Fragments arranged by size:
4215 2525 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4036 4085 6566 882
Size: 617 2537 49 2481 2219
Fragments arranged by size:
2537 2481 2219 617 49
BsaAI yAC'GTr
Cuts at: 493 1204 5694 493
Size: 711 4490 2702
Fragments arranged by size:
4490 2702 711
BsaBI GATnn'nnATC
Cuts at: 7897 7897
Size: 7903
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3848 5710 6092 6727
Size: 53 83 186 936 2315 1862 382 635
Cuts at: 6727 275
Size: 1451
Fragments arranged by size:
2315 1862 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3043 3208 4287 6336 6734 7008 7155 7566 3043
Size: 165 1079 2049 398 274 147 411 3380
Fragments arranged by size:
3380 2049 1079 411 398 274 165 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3799 3829 3835 3865 1178
Size: 30 165 30 2396 30 6 30 5216
Fragments arranged by size:
5216 2396 165 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 4572 5060 1186
Size: 916 2470 488 4029
Fragments arranged by size:
4029 2470 916 488
BseYI C'CCAG_C
Cuts at: 3663 4256 4983 5020 5175 7106 7602 3663
Size: 593 727 37 155 1931 496 3964
Fragments arranged by size:
3964 1931 727 593 496 155 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 2964 1163
Size: 573 429 799 6102
Fragments arranged by size:
6102 799 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 2154 3936 5353 6114 7142
Size: 275 186 30 123 1782 1417 761 1028
Cuts at: 7142 1540
Size: 2301
Fragments arranged by size:
2301 1782 1417 1028 761 275 186 123
30
BsiHKAI G_wGCw'C
Cuts at: 2550 2565 3093 3718 4922 5964 6049 7120 2550
Size: 15 528 625 1204 1042 85 1071 3333
Fragments arranged by size:
3333 1204 1071 1042 625 528 85 15
BsmI GAATG_Cn'
Cuts at: 2257 3984 5414 5507 2257
Size: 1727 1430 93 4653
Fragments arranged by size:
4653 1727 1430 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2550 2565 3093 3718 4843 4922 5222
Size: 399 760 15 528 625 1125 79 300
Cuts at: 5222 5964 6049 7120 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1125 1071 760 742 625 528 399
300 85 79 15
BspEI T'CCGG_A
Cuts at: 7566 7566
Size: 7903
BspHI T'CATG_A
Cuts at: 5792 7522 5792
Size: 1730 6173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2376 3058 3429 5258 844
Size: 1532 682 371 1829 3489
Fragments arranged by size:
3489 1829 1532 682 371
BsrBI CCG'CTC
Cuts at: 1394 1823 5790 1394
Size: 429 3967 3507
Fragments arranged by size:
3967 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3153 4759 4761 6398 6572 65
Size: 1183 1905 1606 2 1637 174 1396
Fragments arranged by size:
1905 1637 1606 1396 1183 174 2
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 3043 3460 6547 6734 1700
Size: 110 237 996 417 3087 187 2869
Fragments arranged by size:
3087 2869 996 417 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 3475 96
Size: 3379 4524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7903
BssSI C'ACGA_G
Cuts at: 2084 3711 5963 6975 2084
Size: 1627 2252 1012 3012
Fragments arranged by size:
3012 2252 1627 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3421 794
Size: 2627 5276
BstBI TT'CG_AA
Cuts at: 5149 6707 5149
Size: 1558 6345
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7903
BstXI CCAn_nnnn'nTGG
Cuts at: 3400 4780 5020 3400
Size: 1380 240 6283
Fragments arranged by size:
6283 1380 240
BstYI r'GATC_y
Cuts at: 1972 2061 2907 3035 3193 3343 3622 3748 4290
Size: 89 846 128 158 150 279 126 542
Cuts at: 4290 4330 4473 5233 5600 5985 6002 7454 7540
Size: 40 143 760 367 385 17 1452 86
Cuts at: 7540 7552 7898 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 846 760 542 385 367 346
279 158 150 143 128 126 89 86
40 17 12
Bsu36I CC'TnA_GG
Cuts at: 3705 4515 3705
Size: 810 7093
BtgI C'CryG_G
Cuts at: 513 1065 2133 3405 3538 3843 513
Size: 552 1068 1272 133 305 4573
Fragments arranged by size:
4573 1272 1068 552 305 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4849 487
Size: 683 56 3623 3541
Fragments arranged by size:
3623 3541 683 56
BtsI GCAGTG_nn'
Cuts at: 3840 4831 4922 5168 5419 6213 6233 3840
Size: 991 91 246 251 794 20 5510
Fragments arranged by size:
5510 991 794 251 246 91 20
ClaI AT'CG_AT
Cuts at: 1554 3168 4113 1554
Size: 1614 945 5344
Fragments arranged by size:
5344 1614 945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4395 4430 529
Size: 35 3831 35 4002
Fragments arranged by size:
4002 3831 35 35
DraI TTT'AAA
Cuts at: 2685 5207 2685
Size: 2522 5381
DraIII CAC_nnn'GTG
Cuts at: 2074 2569 2074
Size: 495 7408
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3542 6910 809
Size: 507 2226 3368 1802
Fragments arranged by size:
3368 2226 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2151 2517
Size: 54 1425 50 275 18 306 15 366
Cuts at: 2517 3048 3390 3402 3447 3462 3573 3933 4227
Size: 531 342 12 45 15 111 360 294
Cuts at: 4227 4488 4896 5350 6239 7868 8
Size: 261 408 454 889 1629 43
Fragments arranged by size:
1629 1425 889 531 454 408 366 360
342 306 294 275 261 111 54 50
45 43 18 15 15 12
EagI C'GGCC_G
Cuts at: 1537 1812 2151 3933 5350 1537
Size: 275 339 1782 1417 4090
Fragments arranged by size:
4090 1782 1417 339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 2798 3213 3867 4647 5833 1353
Size: 857 588 415 654 780 1186 3423
Fragments arranged by size:
3423 1186 857 780 654 588 415
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6490 6864 7010 387
Size: 1064 138 4901 374 146 1280
Fragments arranged by size:
4901 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2548 2548
Size: 7903
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2273 2814 3264 3357 4164 4623
Size: 393 431 210 541 450 93 807 459
Cuts at: 4623 5148 5966 6563 7350 7618 1239
Size: 525 818 597 787 268 1524
Fragments arranged by size:
1524 818 807 787 597 541 525 459
450 431 393 268 210 93
EcoNI CCTnn'n_nnAGG
Cuts at: 4949 4949
Size: 7903
EcoO109I rG'GnC_Cy
Cuts at: 3608 4612 3608
Size: 1004 6899
EcoRI G'AATT_C
Cuts at: 4321 5212 5590 4321
Size: 891 378 6634
Fragments arranged by size:
6634 891 378
EcoRV GAT'ATC
Cuts at: 4469 4469
Size: 7903
FspI TGC'GCA
Cuts at: 6409 6409
Size: 7903
HaeII r_GCGC'y
Cuts at: 1678 3851 7050 1678
Size: 2173 3199 2531
Fragments arranged by size:
3199 2531 2173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 2986 3018 3505
Size: 32 135 32 716 32 666 32 487
Cuts at: 3505 3537 4148 4180 4458 4490 5033 5065 6544
Size: 32 611 32 278 32 543 32 1479
Cuts at: 6544 6576 6618 6650 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1479 716 666 611 543 487 278
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5247 5493 669
Size: 988 281 3309 246 3079
Fragments arranged by size:
3309 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2264 2679 3920 748
Size: 1516 415 1241 4731
Fragments arranged by size:
4731 1516 1241 415
HpaI GTT'AAC
Cuts at: 5493 5493
Size: 7903
KasI G'GCGC_C
Cuts at: 3847 3847
Size: 7903
KpnI G_GTAC'C
Cuts at: 4833 5200 5228 4833
Size: 367 28 7508
Fragments arranged by size:
7508 367 28
MfeI C'AATT_G
Cuts at: 5502 5502
Size: 7903
MluI A'CGCG_T
Cuts at: 7779 7779
Size: 7903
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2155 4152 4455 4534 4585 7017 7201 1305
Size: 850 1997 303 79 51 2432 184 2007
Fragments arranged by size:
2432 2007 1997 850 303 184 79 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 3050 3392 3404 3449
Size: 54 1425 343 687 531 342 12 45
Cuts at: 3449 4229 4490 4898 10
Size: 780 261 408 3015
Fragments arranged by size:
3015 1425 780 687 531 408 343 342
261 54 45 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3508 5066 5861 6220 6379 518
Size: 648 843 1499 1558 795 359 159 2042
Fragments arranged by size:
2042 1558 1499 843 795 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3473 3508 3655 3667 5319 5996 7144 7389
Size: 1867 35 147 12 1652 677 1148 245
Cuts at: 7389 1606
Size: 2120
Fragments arranged by size:
2120 1867 1652 1148 677 245 147 35
12
NarI GG'CG_CC
Cuts at: 3848 3848
Size: 7903
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7903
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7903
NotI GC'GGCC_GC
Cuts at: 5350 5350
Size: 7903
NspI r_CATG'y
Cuts at: 1132 1229 5261 6806 1132
Size: 97 4032 1545 2229
Fragments arranged by size:
4032 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2876 1348
Size: 1528 6375
PciI A'CATG_T
Cuts at: 6802 6802
Size: 7903
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2850 3203 1108
Size: 1742 353 5808
Fragments arranged by size:
5808 1742 353
PfoI T'CCnGG_A
Cuts at: 2040 4455 2040
Size: 2415 5488
PmeI GTTT'AAAC
Cuts at: 5207 5207
Size: 7903
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5972 6004 7485 7517 5972
Size: 32 1481 32 6358
Fragments arranged by size:
6358 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4612 4612
Size: 7903
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7903
PsiI TTA'TAA
Cuts at: 5473 5473
Size: 7903
PspXI vC'TCGA_Gb
Cuts at: 3381 3381
Size: 7903
PstI C_TGCA'G
Cuts at: 830 3417 3426 5255 830
Size: 2587 9 1829 3478
Fragments arranged by size:
3478 2587 1829 9
PvuI CG_AT'CG
Cuts at: 2001 2031 2001
Size: 30 7873
PvuII CAG'CTG
Cuts at: 3473 3655 3667 3473
Size: 182 12 7709
Fragments arranged by size:
7709 182 12
SacI G_AGCT'C
Cuts at: 2550 2550
Size: 7903
SalI G'TCGA_C
Cuts at: 1936 5245 1936
Size: 3309 4594
SapI GCTCTTCn'nnn_
Cuts at: 3213 3213
Size: 7903
SbfI CC_TGCA'GG
Cuts at: 5255 5255
Size: 7903
ScaI AGT'ACT
Cuts at: 6151 7863 6151
Size: 1712 6191
SexAI A'CCwGG_T
Cuts at: 2070 5199 2070
Size: 3129 4774
SfcI C'TryA_G
Cuts at: 826 1045 2703 3413 3422 5251 6386 7067 7258
Size: 219 1658 710 9 1829 1135 681 191
Cuts at: 7258 826
Size: 1471
Fragments arranged by size:
1829 1658 1471 1135 710 681 219 191
9
SfoI GGC'GCC
Cuts at: 3849 3849
Size: 7903
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7903
SgrAI Cr'CCGG_yG
Cuts at: 1700 3043 1700
Size: 1343 6560
SmaI CCC'GGG
Cuts at: 1872 5230 1872
Size: 3358 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2316 3381 3482 6007 6908 7170
Size: 197 926 373 1065 101 2525 901 262
Cuts at: 7170 820
Size: 1553
Fragments arranged by size:
2525 1553 1065 926 901 373 262 197
101
SnaBI TAC'GTA
Cuts at: 493 5694 493
Size: 5201 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7903
SphI G_CATG'C
Cuts at: 1132 5261 1132
Size: 4129 3774
SspI AAT'ATT
Cuts at: 5 52 5827 5
Size: 47 5775 2081
Fragments arranged by size:
5775 2081 47
StuI AGG'CCT
Cuts at: 1517 3992 4627 4874 1517
Size: 2475 635 247 4546
Fragments arranged by size:
4546 2475 635 247
StyI C'CwwG_G
Cuts at: 513 1065 2665 3307 3387 4491 4622 4857 4869
Size: 552 1600 642 80 1104 131 235 12
Cuts at: 4869 4899 5342 513
Size: 30 443 3074
Fragments arranged by size:
3074 1600 1104 642 552 443 235 131
80 30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 3657 4997 5930 5947 6100 6285
Size: 1255 1956 121 1340 933 17 153 185
Cuts at: 6285 6730 325
Size: 445 1498
Fragments arranged by size:
1956 1498 1340 1255 933 445 185 153
121 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3475 6149 7861
Size: 274 80 33 51 1443 1498 2674 1712
Cuts at: 7861 96
Size: 138
Fragments arranged by size:
2674 1712 1498 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3835 3867 7863 7895 3835
Size: 32 3996 32 3843
Fragments arranged by size:
3996 3843 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7903
XbaI T'CTAG_A
Cuts at: 5239 5239
Size: 7903
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3442 3826 4168 4390 5068 3442
Size: 384 342 222 678 6277
Fragments arranged by size:
6277 678 384 342 222
XhoI C'TCGA_G
Cuts at: 1943 2316 3381 1943
Size: 373 1065 6465
Fragments arranged by size:
6465 1065 373
XmaI C'CCGG_G
Cuts at: 1870 5228 1870
Size: 3358 4545
XmnI GAAnn'nnTTC
Cuts at: 3984 4426 6032 3984
Size: 442 1606 5855
Fragments arranged by size:
5855 1606 442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5711 276
Size: 53 83 186 936 4177 2468
Fragments arranged by size:
4177 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BfrBI BplI BsiWI
BsmBI BstZ17I FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI
SfiI SrfI SwaI