Restriction map for
pFN21AB7004
(Circular) (Six-base) MAPSORT of: pFN21AB7004.seq Check: 9784 from: 1 to: 8725
pFN21AB7004 8725 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2293 2293
Size: 8725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6535 278
Size: 53 83 186 936 4999 2468
Fragments arranged by size:
4999 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6068 1937
Size: 4131 4594
Acc65I G'GTAC_C
Cuts at: 5134 6046 5134
Size: 912 7813
AclI AA'CG_TT
Cuts at: 1563 6852 7225 1563
Size: 5289 373 3063
Fragments arranged by size:
5289 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2739 3687 4391 4589 6788 8172 8440 1632
Size: 1107 948 704 198 2199 1384 268 1917
Fragments arranged by size:
2199 1917 1384 1107 948 704 268 198
AfeI AGC'GCT
Cuts at: 2864 2864
Size: 8725
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8528
AflIII A'CryG_T
Cuts at: 1203 2316 7624 8601 1203
Size: 1113 5308 977 1327
Fragments arranged by size:
5308 1327 1113 977
AgeI A'CCGG_T
Cuts at: 7556 7556
Size: 8725
AhdI GACnn_n'nnGTC
Cuts at: 7454 7454
Size: 8725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4361 5338 1184
Size: 3177 977 4571
Fragments arranged by size:
4571 3177 977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4418 4450 4418
Size: 32 8693
AlwNI CAG_nnn'CTG
Cuts at: 2354 3688 4484 5491 5804 6468 8040 2354
Size: 1334 796 1007 313 664 1572 3039
Fragments arranged by size:
3039 1572 1334 1007 796 664 313
ApaI G_GGCC'C
Cuts at: 2697 3048 3836 3890 4013 4124 4200 4478 2697
Size: 351 788 54 123 111 76 278 6944
Fragments arranged by size:
6944 788 351 278 123 111 76 54
ApaLI G'TGCA_C
Cuts at: 6782 7938 6782
Size: 1156 7569
ApoI r'AATT_y
Cuts at: 1493 2138 2521 2918 3592 3870 6034 6260 6412
Size: 645 383 397 674 278 2164 226 152
Cuts at: 6412 8521 1493
Size: 2109 1697
Fragments arranged by size:
2164 2109 1697 674 645 397 383 278
226 152
AseI AT'TA_AT
Cuts at: 160 1032 7279 160
Size: 872 6247 1606
Fragments arranged by size:
6247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8725
AvaI C'yCGr_G
Cuts at: 1870 1943 3182 3303 3418 4420 5339 5505 5952
Size: 73 1239 121 115 1002 919 166 447
Cuts at: 5952 6050 1870
Size: 98 4545
Fragments arranged by size:
4545 1239 1002 919 447 166 121 115
98 73
AvrII C'CTAG_G
Cuts at: 3028 3028
Size: 8725
BamHI G'GATC_C
Cuts at: 3962 4617 6055 6422 3962
Size: 655 1438 367 6265
Fragments arranged by size:
6265 1438 655 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2566 3361 4075 5134 6046
Size: 325 217 627 779 795 714 1059 912
Cuts at: 6046 7501 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1059 912 795 779 714 627
325 217
BanII G_rGCy'C
Cuts at: 1391 2036 2280 2697 2805 3048 3067 3836 3890
Size: 645 244 417 108 243 19 769 54
Cuts at: 3890 4013 4124 4200 4415 4478 5850 5876 6044
Size: 123 111 76 215 63 1372 26 168
Cuts at: 6044 1391
Size: 4072
Fragments arranged by size:
4072 1372 769 645 417 244 243 215
168 123 111 108 76 63 54 26
19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 2300 4733 928
Size: 971 333 68 2433 4920
Fragments arranged by size:
4920 2433 971 333 68
BbvCI CC'TCA_GC
Cuts at: 4976 4976
Size: 8725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3284 3318 6916 6950 1903
Size: 34 50 34 1263 34 3598 34 3678
Fragments arranged by size:
3678 3598 1263 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6617 7827 6617
Size: 1210 7515
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8725
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 8725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3011 4041 5277
Size: 107 122 71 631 605 1339 1030 1236
Cuts at: 5277 5845 7336 136
Size: 568 1491 1525
Fragments arranged by size:
1525 1491 1339 1236 1030 631 605 568
122 107 71
BglII A'GATC_T
Cuts at: 5401 8720 5401
Size: 3319 5406
BlpI GC'TnA_GC
Cuts at: 4062 6142 4062
Size: 2080 6645
Bme1580I G_kGCm'C
Cuts at: 1790 2697 3048 3364 3836 3890 4013 4124 4200
Size: 907 351 316 472 54 123 111 76
Cuts at: 4200 4478 6786 7942 1790
Size: 278 2308 1156 2573
Fragments arranged by size:
2573 2308 1156 907 472 351 316 278
123 111 76 54
BmgBI CAC'GTC
Cuts at: 1318 2597 3058 5971 1318
Size: 1279 461 2913 4072
Fragments arranged by size:
4072 2913 1279 461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2700 2822 3133
Size: 416 53 83 399 24 1283 122 311
Cuts at: 3133 4833 5214 6456 7414 442
Size: 1700 381 1242 958 1753
Fragments arranged by size:
1753 1700 1283 1242 958 416 399 381
311 122 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2052 2052
Size: 8725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2870 3090 3257 3567 4272 4755 4887
Size: 1044 587 220 167 310 705 483 132
Cuts at: 4887 5157 5352 5445 5862 7385 1239
Size: 270 195 93 417 1523 2579
Fragments arranged by size:
2579 1523 1044 705 587 483 417 310
270 220 195 167 132 93
Bpu10I CC'TnA_GC
Cuts at: 4976 4976
Size: 8725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3063 3923 4415 4529 5124 6850 7715 8013 3063
Size: 860 492 114 595 1726 865 298 3775
Fragments arranged by size:
3775 1726 865 860 595 492 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3713 5689 7388 882
Size: 617 2214 1976 1699 2219
Fragments arranged by size:
2219 2214 1976 1699 617
BsaAI yAC'GTr
Cuts at: 493 1204 2317 6516 493
Size: 711 1113 4199 2702
Fragments arranged by size:
4199 2702 1113 711
BsaBI GATnn'nnATC
Cuts at: 3942 8719 3942
Size: 4777 3948
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6532 6914 7549 275
Size: 53 83 186 936 4999 382 635 1451
Fragments arranged by size:
4999 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5432 5644 7158 7556 7830 7977 8388 5432
Size: 212 1514 398 274 147 411 5769
Fragments arranged by size:
5769 1514 411 398 274 212 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4270 4300 4573 4603 4753
Size: 30 165 30 2867 30 273 30 150
Cuts at: 4753 4783 1178
Size: 30 5120
Fragments arranged by size:
5120 2867 273 165 150 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 3247 4031 4275 5046 1186
Size: 858 1203 784 244 771 4865
Fragments arranged by size:
4865 1203 858 784 771 244
BseYI C'CCAG_C
Cuts at: 2124 2190 2497 3357 3703 3743 3777 4001 4101
Size: 66 307 860 346 40 34 224 100
Cuts at: 4101 4408 4477 4657 4666 4995 5285 5480 7928
Size: 307 69 180 9 329 290 195 2448
Cuts at: 7928 8424 2124
Size: 496 2425
Fragments arranged by size:
2448 2425 860 496 346 329 307 307
290 224 195 180 100 69 66 40
34 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6175 6936 7964 1540
Size: 275 186 4174 761 1028 2301
Fragments arranged by size:
4174 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 2856 2932 3067 4098 6786 6871 7942 2036
Size: 820 76 135 1031 2688 85 1071 2819
Fragments arranged by size:
2819 2688 1071 1031 820 135 85 76
BsmI GAATG_Cn'
Cuts at: 2008 4348 6236 6329 2008
Size: 2340 1888 93 4404
Fragments arranged by size:
4404 2340 1888 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2389 2389
Size: 8725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2280 2697 2805 2856 2932 3048
Size: 399 246 244 417 108 51 76 116
Cuts at: 3048 3067 3364 3836 3890 4013 4098 4124 4200
Size: 19 297 472 54 123 85 26 76
Cuts at: 4200 4415 4478 5850 5876 6044 6786 6871 7942
Size: 215 63 1372 26 168 742 85 1071
Cuts at: 7942 1391
Size: 2174
Fragments arranged by size:
2174 1372 1071 742 472 417 399 297
246 244 215 168 123 116 108 85
85 76 76 63 54 51 26 26
19
BspEI T'CCGG_A
Cuts at: 5644 8388 5644
Size: 2744 5981
BspHI T'CATG_A
Cuts at: 5928 6614 8344 5928
Size: 686 1730 6309
Fragments arranged by size:
6309 1730 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2293 5185 5301 6080 844
Size: 1449 2892 116 779 3489
Fragments arranged by size:
3489 2892 1449 779 116
BsrBI CCG'CTC
Cuts at: 1394 1823 6612 1394
Size: 429 4789 3507
Fragments arranged by size:
4789 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4839 7220 7394 65
Size: 1183 3591 2381 174 1396
Fragments arranged by size:
3591 2381 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4218 7369 7556 1700
Size: 110 2408 3151 187 2869
Fragments arranged by size:
3151 2869 2408 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8725
BssSI C'ACGA_G
Cuts at: 6785 7797 6785
Size: 1012 7713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8725
BstBI TT'CG_AA
Cuts at: 7529 7529
Size: 8725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8725
BstXI CCAn_nnnn'nTGG
Cuts at: 5543 5999 5543
Size: 456 8269
BstYI r'GATC_y
Cuts at: 1972 2761 3962 4617 5401 6055 6422 6807 6824
Size: 789 1201 655 784 654 367 385 17
Cuts at: 6824 8276 8362 8374 8720 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1201 789 784 655 654 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4014 4014
Size: 8725
BtgI C'CryG_G
Cuts at: 513 1065 2254 3726 4647 4956 5037 5808 513
Size: 552 1189 1472 921 309 81 771 3430
Fragments arranged by size:
3430 1472 1189 921 771 552 309 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4434 487
Size: 683 56 3208 4778
Fragments arranged by size:
4778 3208 683 56
BtsI GCAGTG_nn'
Cuts at: 2672 2727 3269 4079 6241 7035 7055 2672
Size: 55 542 810 2162 794 20 4342
Fragments arranged by size:
4342 2162 810 794 542 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4938 4973 5019 5054 529
Size: 35 4374 35 46 35 4200
Fragments arranged by size:
4374 4200 46 35 35 35
DraI TTT'AAA
Cuts at: 6029 6029
Size: 8725
DraIII CAC_nnn'GTG
Cuts at: 2936 2936
Size: 8725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3284 7732 809
Size: 507 1968 4448 1802
Fragments arranged by size:
4448 1968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2257 2334 2505
Size: 54 1425 50 275 18 427 77 171
Cuts at: 2505 3557 3604 3628 3723 4878 5278 5773 6172
Size: 1052 47 24 95 1155 400 495 399
Cuts at: 6172 7061 8690 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1155 1052 889 495 427 400
399 275 171 95 77 54 50 47
43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 6172 1537
Size: 275 4360 4090
Fragments arranged by size:
4360 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2758 3219 3224 3663 4591 6655 1353
Size: 1405 461 5 439 928 2064 3423
Fragments arranged by size:
3423 2064 1405 928 461 439 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2766 4324 7312 7686 7832 387
Size: 1064 138 1177 1558 2988 374 146 1280
Fragments arranged by size:
2988 1558 1280 1177 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2034 3065 2034
Size: 1031 7694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2739 2870 3090 3257 3567 3687
Size: 393 651 456 131 220 167 310 120
Cuts at: 3687 4272 4391 4589 4755 4887 5157 5352 5445
Size: 585 119 198 166 132 270 195 93
Cuts at: 5445 5862 6788 7385 8172 8440 1239
Size: 417 926 597 787 268 1524
Fragments arranged by size:
1524 926 787 651 597 585 456 417
393 310 270 268 220 198 195 167
166 132 131 120 119 93
EcoNI CCTnn'n_nnAGG
Cuts at: 2646 2837 3032 3351 3630 2646
Size: 191 195 319 279 7741
Fragments arranged by size:
7741 319 279 195 191
EcoO109I rG'GnC_Cy
Cuts at: 3038 3045 3155 3320 3832 3833 3887 4010 4121
Size: 7 110 165 512 1 54 123 111
Cuts at: 4121 5042 5065 5075 5115 5270 5336 5345 5540
Size: 921 23 10 40 155 66 9 195
Cuts at: 5540 5729 5837 5912 5949 5996 3038
Size: 189 108 75 37 47 5767
Fragments arranged by size:
5767 921 512 195 189 165 155 123
111 110 108 75 66 54 47 40
37 23 10 9 7 1
EcoRI G'AATT_C
Cuts at: 2521 2918 3870 6034 6412 2521
Size: 397 952 2164 378 4834
Fragments arranged by size:
4834 2164 952 397 378
EcoRV GAT'ATC
Cuts at: 2454 2887 2454
Size: 433 8292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5291 5291
Size: 8725
FspI TGC'GCA
Cuts at: 7231 7231
Size: 8725
HaeII r_GCGC'y
Cuts at: 1678 2438 2866 3784 7872 1678
Size: 760 428 918 4088 2531
Fragments arranged by size:
4088 2531 918 760 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2074 2106 2819 2851 3274
Size: 32 135 32 502 32 713 32 423
Cuts at: 3274 3306 3967 3999 4573 4605 4880 4912 5011
Size: 32 661 32 574 32 275 32 99
Cuts at: 5011 5043 5122 5154 5716 5748 5779 5811 7366
Size: 32 79 32 562 32 31 32 1555
Cuts at: 7366 7398 7440 7472 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1555 713 661 574 562 502 423
275 135 99 79 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 3955 6069 6315 669
Size: 988 281 2017 2114 246 3079
Fragments arranged by size:
3079 2114 2017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8725
HpaI GTT'AAC
Cuts at: 6315 6315
Size: 8725
KpnI G_GTAC'C
Cuts at: 5138 6050 5138
Size: 912 7813
MfeI C'AATT_G
Cuts at: 4191 6324 4191
Size: 2133 6592
MluI A'CGCG_T
Cuts at: 8601 8601
Size: 8725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3990 5443 5737 7839 8023 1305
Size: 2685 1453 294 2102 184 2007
Fragments arranged by size:
2685 2102 2007 1453 294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2259 2336 3559 3606 3725
Size: 54 1425 343 427 77 1223 47 119
Cuts at: 3725 4880 10
Size: 1155 3855
Fragments arranged by size:
3855 1425 1223 1155 427 343 119 77
54 47
MslI CAynn'nnrTG
Cuts at: 518 1166 3090 3752 4576 5786 5888 6683 7042
Size: 648 1924 662 824 1210 102 795 359
Cuts at: 7042 7201 518
Size: 159 2042
Fragments arranged by size:
2042 1924 1210 824 795 662 648 359
159 102
MspA1I CmG'CkG
Cuts at: 1606 2128 2848 3621 3688 3703 4456 4481 5801
Size: 522 720 773 67 15 753 25 1320
Cuts at: 5801 6141 6818 7966 8211 1606
Size: 340 677 1148 245 2120
Fragments arranged by size:
2120 1320 1148 773 753 720 677 522
340 245 67 25 15
NaeI GCC'GGC
Cuts at: 4220 4220
Size: 8725
NcoI C'CATG_G
Cuts at: 513 1065 2254 3726 4647 513
Size: 552 1189 1472 921 4591
Fragments arranged by size:
4591 1472 1189 921 552
NdeI CA'TA_TG
Cuts at: 387 3174 4230 387
Size: 2787 1056 4882
Fragments arranged by size:
4882 2787 1056
NgoMIV G'CCGG_C
Cuts at: 4218 4218
Size: 8725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8725
NotI GC'GGCC_GC
Cuts at: 6172 6172
Size: 8725
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 8725
NspI r_CATG'y
Cuts at: 1132 1229 2899 3403 3448 4054 4468 5380 5566
Size: 97 1670 504 45 606 414 912 186
Cuts at: 5566 6083 7628 1132
Size: 517 1545 2229
Fragments arranged by size:
2229 1670 1545 912 606 517 504 414
186 97 45
PasI CC'CwG_GG
Cuts at: 1348 2737 3716 5161 5536 5861 1348
Size: 1389 979 1445 375 325 4212
Fragments arranged by size:
4212 1445 1389 979 375 325
PciI A'CATG_T
Cuts at: 7624 7624
Size: 8725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8725
PfoI T'CCnGG_A
Cuts at: 3273 5542 3273
Size: 2269 6456
PmeI GTTT'AAAC
Cuts at: 6029 6029
Size: 8725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4784 4816 5499 5531 6794 6826 8307 8339 4784
Size: 32 683 32 1263 32 1481 32 5170
Fragments arranged by size:
5170 1481 1263 683 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3038 3155 3320 5042 5065 5115 5345 5540 5729
Size: 117 165 1722 23 50 230 195 189
Cuts at: 5729 5912 5996 3038
Size: 183 84 5767
Fragments arranged by size:
5767 1722 230 195 189 183 165 117
84 50 23
PshAI GACnn'nnGTC
Cuts at: 1613 2729 1613
Size: 1116 7609
PsiI TTA'TAA
Cuts at: 6295 6295
Size: 8725
PspOMI G'GGCC_C
Cuts at: 2693 3044 3832 3886 4009 4120 4196 4474 2693
Size: 351 788 54 123 111 76 278 6944
Fragments arranged by size:
6944 788 351 278 123 111 76 54
PspXI vC'TCGA_Gb
Cuts at: 5505 5505
Size: 8725
PstI C_TGCA'G
Cuts at: 830 2350 2547 3210 5409 6077 830
Size: 1520 197 663 2199 668 3478
Fragments arranged by size:
3478 2199 1520 668 663 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8725
PvuII CAG'CTG
Cuts at: 2128 2848 3688 3703 4456 4481 5801 2128
Size: 720 840 15 753 25 1320 5052
Fragments arranged by size:
5052 1320 840 753 720 25 15
SacI G_AGCT'C
Cuts at: 2036 3067 2036
Size: 1031 7694
SalI G'TCGA_C
Cuts at: 1936 6067 1936
Size: 4131 4594
SanDI GG'GwC_CC
Cuts at: 5042 5065 5115 5912 5042
Size: 23 50 797 7855
Fragments arranged by size:
7855 797 50 23
SapI GCTCTTCn'nnn_
Cuts at: 2758 2758
Size: 8725
SbfI CC_TGCA'GG
Cuts at: 6077 6077
Size: 8725
ScaI AGT'ACT
Cuts at: 6973 8685 6973
Size: 1712 7013
SexAI A'CCwGG_T
Cuts at: 3535 3535
Size: 8725
SfcI C'TryA_G
Cuts at: 826 1045 2097 2226 2346 2543 2646 3206 4701
Size: 219 1052 129 120 197 103 560 1495
Cuts at: 4701 5405 6073 7208 7889 8080 826
Size: 704 668 1135 681 191 1471
Fragments arranged by size:
1495 1471 1135 1052 704 681 668 560
219 197 191 129 120 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 3011 5277 3011
Size: 2266 6459
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8725
SmaI CCC'GGG
Cuts at: 1872 5341 5954 6052 1872
Size: 3469 613 98 4545
Fragments arranged by size:
4545 3469 613 98
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3078 3902 4430 4544 5139 5505
Size: 197 926 1135 824 528 114 595 366
Cuts at: 5505 6829 7730 7992 820
Size: 1324 901 262 1553
Fragments arranged by size:
1553 1324 1135 926 901 824 595 528
366 262 197 114
SnaBI TAC'GTA
Cuts at: 493 6516 493
Size: 6023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8725
SphI G_CATG'C
Cuts at: 1132 2899 4468 5380 6083 1132
Size: 1767 1569 912 703 3774
Fragments arranged by size:
3774 1767 1569 912 703
SspI AAT'ATT
Cuts at: 5 52 5498 6649 5
Size: 47 5446 1151 2081
Fragments arranged by size:
5446 2081 1151 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8725
StyI C'CwwG_G
Cuts at: 513 1065 2254 3028 3655 3726 4317 4647 5275
Size: 552 1189 774 627 71 591 330 628
Cuts at: 5275 5992 6164 513
Size: 717 172 3074
Fragments arranged by size:
3074 1189 774 717 628 627 591 552
330 172 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2572 2590 5870 5969 6752 6769 6922
Size: 1255 992 18 3280 99 783 17 153
Cuts at: 6922 7107 7552 325
Size: 185 445 1498
Fragments arranged by size:
3280 1498 1255 992 783 445 185 153
99 18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4751 5176 6971
Size: 274 80 33 51 1443 2774 425 1795
Cuts at: 6971 8683 96
Size: 1712 138
Fragments arranged by size:
2774 1795 1712 1443 425 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2162 2194 4270 4302 8685 8717 2162
Size: 32 2076 32 4383 32 2170
Fragments arranged by size:
4383 2170 2076 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3537 1316
Size: 2221 6504
XbaI T'CTAG_A
Cuts at: 3530 6061 3530
Size: 2531 6194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3791 4109 3791
Size: 318 8407
XhoI C'TCGA_G
Cuts at: 1943 5505 1943
Size: 3562 5163
XmaI C'CCGG_G
Cuts at: 1870 5339 5952 6050 1870
Size: 3469 613 98 4545
Fragments arranged by size:
4545 3469 613 98
XmnI GAAnn'nnTTC
Cuts at: 2918 3117 6854 2918
Size: 199 3737 4789
Fragments arranged by size:
4789 3737 199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6533 276
Size: 53 83 186 936 4999 2468
Fragments arranged by size:
4999 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbeI BsiWI BstZ17I FseI FspAI KasI
NarI NruI PacI PmlI PsrI RsrII SacII SfoI
SrfI SwaI