Restriction map for
pFN21AB7003
(Circular) (Six-base) MAPSORT of: pFN21AB7003.seq Check: 5910 from: 1 to: 8566
pFN21AB7003 8566 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3538 3804 4403 3538
Size: 266 599 7701
Fragments arranged by size:
7701 599 266
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6376 278
Size: 53 83 186 936 4840 2468
Fragments arranged by size:
4840 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4883 5909 1937
Size: 2946 1026 4594
Fragments arranged by size:
4594 2946 1026
Acc65I G'GTAC_C
Cuts at: 2068 5887 2068
Size: 3819 4747
AclI AA'CG_TT
Cuts at: 1563 3807 4929 6693 7066 1563
Size: 2244 1122 1764 373 3063
Fragments arranged by size:
3063 2244 1764 1122 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 3749 3927 4196 6629 8013 8281 1632
Size: 793 1324 178 269 2433 1384 268 1917
Fragments arranged by size:
2433 1917 1384 1324 793 269 268 178
AfeI AGC'GCT
Cuts at: 3073 3073
Size: 8566
AflII C'TTAA_G
Cuts at: 820 1017 5000 5161 820
Size: 197 3983 161 4225
Fragments arranged by size:
4225 3983 197 161
AflIII A'CryG_T
Cuts at: 1203 5346 7465 8442 1203
Size: 4143 2119 977 1327
Fragments arranged by size:
4143 2119 1327 977
AgeI A'CCGG_T
Cuts at: 7397 7397
Size: 8566
AhdI GACnn_n'nnGTC
Cuts at: 7295 7295
Size: 8566
AleI CACnn'nnGTG
Cuts at: 1166 2612 3939 5045 5079 1166
Size: 1446 1327 1106 34 4653
Fragments arranged by size:
4653 1446 1327 1106 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8566
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5462 5494 5462
Size: 32 8534
AlwNI CAG_nnn'CTG
Cuts at: 2891 3278 3629 4249 6309 7881 2891
Size: 387 351 620 2060 1572 3576
Fragments arranged by size:
3576 2060 1572 620 387 351
ApaLI G'TGCA_C
Cuts at: 2097 6623 7779 2097
Size: 4526 1156 2884
Fragments arranged by size:
4526 2884 1156
ApoI r'AATT_y
Cuts at: 1493 2227 2558 2787 2896 3121 3856 4461 4480
Size: 734 331 229 109 225 735 605 19
Cuts at: 4480 4592 4812 5809 5875 6101 6253 8362 1493
Size: 112 220 997 66 226 152 2109 1697
Fragments arranged by size:
2109 1697 997 735 734 605 331 229
226 225 220 152 112 109 66 19
AseI AT'TA_AT
Cuts at: 160 1032 4497 5298 5538 7120 160
Size: 872 3465 801 240 1582 1606
Fragments arranged by size:
3465 1606 1582 872 801 240
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8566
AvaI C'yCGr_G
Cuts at: 1870 1943 5891 1870
Size: 73 3948 4545
Fragments arranged by size:
4545 3948 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5568 5601 5568
Size: 33 8533
BamHI G'GATC_C
Cuts at: 3884 5896 6263 3884
Size: 2012 367 6187
Fragments arranged by size:
6187 2012 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 4724 5819 5887 7342
Size: 325 217 627 281 2656 1095 68 1455
Cuts at: 7342 618
Size: 1842
Fragments arranged by size:
2656 1842 1455 1095 627 325 281 217
68
BanII G_rGCy'C
Cuts at: 1391 5885 1391
Size: 4494 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2189 3132 3684 3757 4290 4309 4329
Size: 971 290 943 552 73 533 19 20
Cuts at: 4329 4508 928
Size: 179 4986
Fragments arranged by size:
4986 971 943 552 533 290 179 73
20 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6757 6791 1903
Size: 34 4820 34 3678
Fragments arranged by size:
4820 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3666 4624 6458 7668 3666
Size: 958 1834 1210 4564
Fragments arranged by size:
4564 1834 1210 958
BclI T'GATC_A
Cuts at: 1548 3178 1548
Size: 1630 6936
BfrBI ATG'CAT
Cuts at: 3968 4169 5094 3968
Size: 201 925 7440
Fragments arranged by size:
7440 925 201
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7177 136
Size: 107 122 71 631 605 5505 1525
Fragments arranged by size:
5505 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8561 8561
Size: 8566
BlpI GC'TnA_GC
Cuts at: 2211 5983 2211
Size: 3772 4794
Bme1580I G_kGCm'C
Cuts at: 1790 2101 6627 7783 1790
Size: 311 4526 1156 2573
Fragments arranged by size:
4526 2573 1156 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4551 6297 7255
Size: 416 53 83 399 24 3134 1746 958
Cuts at: 7255 442
Size: 1753
Fragments arranged by size:
3134 1753 1746 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4395 1056
Size: 3339 5227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4450 4450
Size: 8566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 3465 4146 7226 1239
Size: 903 1323 681 3080 2579
Fragments arranged by size:
3080 2579 1323 903 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2379 2664 2677 3478 4018 6691 7556 7854 2379
Size: 285 13 801 540 2673 865 298 3091
Fragments arranged by size:
3091 2673 865 801 540 298 285 13
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3994 7229 882
Size: 617 2495 3235 2219
Fragments arranged by size:
3235 2495 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6357 493
Size: 711 5153 2702
Fragments arranged by size:
5153 2702 711
BsaBI GATnn'nnATC
Cuts at: 4170 8560 4170
Size: 4390 4176
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6373 6755 7390 275
Size: 53 83 186 936 4840 382 635 1451
Fragments arranged by size:
4840 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6999 7397 7671 7818 8229 6999
Size: 398 274 147 411 7336
Fragments arranged by size:
7336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8341
Fragments arranged by size:
8341 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8566
BseYI C'CCAG_C
Cuts at: 3405 7769 8265 3405
Size: 4364 496 3706
Fragments arranged by size:
4364 3706 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7993
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5258 6016 6777 7805 1540
Size: 275 186 3257 758 761 1028 2301
Fragments arranged by size:
3257 2301 1028 761 758 275 186
BsiHKAI G_wGCw'C
Cuts at: 2101 4385 6627 6712 7783 2101
Size: 2284 2242 85 1071 2884
Fragments arranged by size:
2884 2284 2242 1071 85
BsmI GAATG_Cn'
Cuts at: 2333 2954 3922 3968 4411 4916 6077 6170 2333
Size: 621 968 46 443 505 1161 93 4729
Fragments arranged by size:
4729 1161 968 621 505 443 93 46
BsmBI CGTCTCn'nnnn_
Cuts at: 5846 5846
Size: 8566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 4385 5885 6627 6712 7783 1391
Size: 399 311 2284 1500 742 85 1071 2174
Fragments arranged by size:
2284 2174 1500 1071 742 399 311 85
BspEI T'CCGG_A
Cuts at: 8229 8229
Size: 8566
BspHI T'CATG_A
Cuts at: 3598 4906 6455 8185 3598
Size: 1308 1549 1730 3979
Fragments arranged by size:
3979 1730 1549 1308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3538 3804 4403 5921 844
Size: 2694 266 599 1518 3489
Fragments arranged by size:
3489 2694 1518 599 266
BsrBI CCG'CTC
Cuts at: 1394 1823 5157 6453 1394
Size: 429 3334 1296 3507
Fragments arranged by size:
3507 3334 1296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3395 4371 4575 7061 7235 65
Size: 1183 2147 976 204 2486 174 1396
Fragments arranged by size:
2486 2147 1396 1183 976 204 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7210 7397 1700
Size: 110 5400 187 2869
Fragments arranged by size:
5400 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3640 96
Size: 3544 5022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8566
BssSI C'ACGA_G
Cuts at: 6626 7638 6626
Size: 1012 7554
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4110 4774 4783 794
Size: 3316 664 9 4577
Fragments arranged by size:
4577 3316 664 9
BstBI TT'CG_AA
Cuts at: 5695 7370 5695
Size: 1675 6891
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8566
BstXI CCAn_nnnn'nTGG
Cuts at: 5086 5176 5086
Size: 90 8476
BstYI r'GATC_y
Cuts at: 1972 3261 3884 5896 6263 6648 6665 8117 8203
Size: 1289 623 2012 367 385 17 1452 86
Cuts at: 8203 8215 8561 1972
Size: 12 346 1977
Fragments arranged by size:
2012 1977 1452 1289 623 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2823 4005 5474 2823
Size: 1182 1469 5915
Fragments arranged by size:
5915 1469 1182
BtgI C'CryG_G
Cuts at: 513 1065 2004 2715 513
Size: 552 939 711 6364
Fragments arranged by size:
6364 939 711 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4035 487
Size: 683 56 2809 5018
Fragments arranged by size:
5018 2809 683 56
BtsI GCAGTG_nn'
Cuts at: 3508 3782 5115 5404 5691 6082 6876 6896 3508
Size: 274 1333 289 287 391 794 20 5178
Fragments arranged by size:
5178 1333 794 391 289 287 274 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2151 2186 4950 4985 529
Size: 35 1587 35 2764 35 4110
Fragments arranged by size:
4110 2764 1587 35 35 35
DraI TTT'AAA
Cuts at: 2454 2602 5220 5870 2454
Size: 148 2618 650 5150
Fragments arranged by size:
5150 2618 650 148
DraIII CAC_nnn'GTG
Cuts at: 4110 4110
Size: 8566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7573 809
Size: 507 6257 1802
Fragments arranged by size:
6257 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4672 6013 6902
Size: 54 1425 50 275 18 2842 1341 889
Cuts at: 6902 8531 8
Size: 1629 43
Fragments arranged by size:
2842 1629 1425 1341 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6013 1537
Size: 275 4201 4090
Fragments arranged by size:
4201 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 3645 4560 4627 5369 5412 6496 1353
Size: 1008 1284 915 67 742 43 1084 3423
Fragments arranged by size:
3423 1284 1084 1008 915 742 67 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7153 7527 7673 387
Size: 1064 138 5564 374 146 1280
Fragments arranged by size:
5564 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2425 3465 3749 3927 4146 4196
Size: 393 510 283 1040 284 178 219 50
Cuts at: 4196 6629 7226 8013 8281 1239
Size: 2433 597 787 268 1524
Fragments arranged by size:
2433 1524 1040 787 597 510 393 284
283 268 219 178 50
EcoRI G'AATT_C
Cuts at: 5809 5875 6253 5809
Size: 66 378 8122
Fragments arranged by size:
8122 378 66
EcoRV GAT'ATC
Cuts at: 2708 2708
Size: 8566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4086 4086
Size: 8566
FspI TGC'GCA
Cuts at: 7072 7072
Size: 8566
HaeII r_GCGC'y
Cuts at: 1678 3075 7713 1678
Size: 1397 4638 2531
Fragments arranged by size:
4638 2531 1397
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 2281 2313 2511
Size: 32 135 32 543 32 134 32 198
Cuts at: 2511 2543 2650 2682 3170 3202 3760 3792 3887
Size: 32 107 32 488 32 558 32 95
Cuts at: 3887 3919 4865 4897 5370 5402 7207 7239 7281
Size: 32 946 32 473 32 1805 32 42
Cuts at: 7281 7313 1373
Size: 32 2626
Fragments arranged by size:
2626 1805 946 558 543 488 473 198
135 134 107 95 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5637 5910 6156 669
Size: 988 281 3699 273 246 3079
Fragments arranged by size:
3699 3079 988 281 273 246
HindIII A'AGCT_T
Cuts at: 748 2165 748
Size: 1417 7149
HpaI GTT'AAC
Cuts at: 6156 6156
Size: 8566
KpnI G_GTAC'C
Cuts at: 2072 5891 2072
Size: 3819 4747
MfeI C'AATT_G
Cuts at: 6165 6165
Size: 8566
MluI A'CGCG_T
Cuts at: 8442 8442
Size: 8566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2215 2660 2727 3276 3289 3862 4215 4641
Size: 910 445 67 549 13 573 353 426
Cuts at: 4641 7680 7864 1305
Size: 3039 184 2007
Fragments arranged by size:
3039 2007 910 573 549 445 426 353
184 67 13
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4674 10
Size: 54 1425 343 2842 3902
Fragments arranged by size:
3902 2842 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2092 2612 3939 4376 4580 5045 5079
Size: 648 926 520 1327 437 204 465 34
Cuts at: 5079 6524 6883 7042 518
Size: 1445 359 159 2042
Fragments arranged by size:
2042 1445 1327 926 648 520 465 437
359 204 159 34
MspA1I CmG'CkG
Cuts at: 1606 4678 5233 5982 6659 7807 8052 1606
Size: 3072 555 749 677 1148 245 2120
Fragments arranged by size:
3072 2120 1148 749 677 555 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2715 513
Size: 552 939 711 6364
Fragments arranged by size:
6364 939 711 552
NdeI CA'TA_TG
Cuts at: 387 4077 387
Size: 3690 4876
NheI G'CTAG_C
Cuts at: 1052 4391 1052
Size: 3339 5227
NotI GC'GGCC_GC
Cuts at: 6013 6013
Size: 8566
NsiI A_TGCA'T
Cuts at: 3970 4171 5096 3970
Size: 201 925 7440
Fragments arranged by size:
7440 925 201
NspI r_CATG'y
Cuts at: 1132 1229 4117 4699 4772 5094 5134 5350 5924
Size: 97 2888 582 73 322 40 216 574
Cuts at: 5924 7469 1132
Size: 1545 2229
Fragments arranged by size:
2888 2229 1545 582 574 322 216 97
73 40
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8566
PciI A'CATG_T
Cuts at: 5346 7465 5346
Size: 2119 6447
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8566
PmeI GTTT'AAAC
Cuts at: 5870 5870
Size: 8566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6635 6667 8148 8180 6635
Size: 32 1481 32 7021
Fragments arranged by size:
7021 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8566
PsiI TTA'TAA
Cuts at: 2697 6136 2697
Size: 3439 5127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3753 3785 4635 4667 3753
Size: 32 850 32 7652
Fragments arranged by size:
7652 850 32 32
PstI C_TGCA'G
Cuts at: 830 5686 5918 830
Size: 4856 232 3478
Fragments arranged by size:
4856 3478 232
PvuI CG_AT'CG
Cuts at: 2001 5258 2001
Size: 3257 5309
PvuII CAG'CTG
Cuts at: 4678 5233 4678
Size: 555 8011
SalI G'TCGA_C
Cuts at: 1936 5908 1936
Size: 3972 4594
SbfI CC_TGCA'GG
Cuts at: 5918 5918
Size: 8566
ScaI AGT'ACT
Cuts at: 6814 8526 6814
Size: 1712 6854
SexAI A'CCwGG_T
Cuts at: 2045 2045
Size: 8566
SfcI C'TryA_G
Cuts at: 826 1045 2152 2462 2815 3990 5202 5682 5914
Size: 219 1107 310 353 1175 1212 480 232
Cuts at: 5914 7049 7730 7921 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1212 1175 1135 1107 681 480 353
310 232 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8566
SmaI CCC'GGG
Cuts at: 1872 5893 1872
Size: 4021 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2394 2656 2679 3457 3997 5000
Size: 197 926 451 262 23 778 540 1003
Cuts at: 5000 5161 6670 7571 7833 820
Size: 161 1509 901 262 1553
Fragments arranged by size:
1553 1509 1003 926 901 778 540 451
262 262 197 161 23
SnaBI TAC'GTA
Cuts at: 493 6357 493
Size: 5864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8566
SphI G_CATG'C
Cuts at: 1132 4117 5094 5924 1132
Size: 2985 977 830 3774
Fragments arranged by size:
3774 2985 977 830
SspI AAT'ATT
Cuts at: 5 52 2674 4042 5216 5456 5596 6490 5
Size: 47 2622 1368 1174 240 140 894 2081
Fragments arranged by size:
2622 2081 1368 1174 894 240 140 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8566
StyI C'CwwG_G
Cuts at: 513 1065 2004 2490 2715 2760 4863 5017 6005
Size: 552 939 486 225 45 2103 154 988
Cuts at: 6005 513
Size: 3074
Fragments arranged by size:
3074 2103 988 939 552 486 225 154
45
SwaI ATTT'AAAT
Cuts at: 5220 5220
Size: 8566
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3951 4138 6593 6610 6763 6948 7393
Size: 1255 2371 187 2455 17 153 185 445
Cuts at: 7393 325
Size: 1498
Fragments arranged by size:
2455 2371 1498 1255 445 187 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3640 3957 6812
Size: 274 80 33 51 1443 1663 317 2855
Cuts at: 6812 8524 96
Size: 1712 138
Fragments arranged by size:
2855 1712 1663 1443 317 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4501 4533 5707 5739 8526 8558 4501
Size: 32 1174 32 2787 32 4509
Fragments arranged by size:
4509 2787 1174 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4132 1316
Size: 2816 5750
XbaI T'CTAG_A
Cuts at: 5902 5902
Size: 8566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2542 2542
Size: 8566
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8566
XmaI C'CCGG_G
Cuts at: 1870 5891 1870
Size: 4021 4545
XmnI GAAnn'nnTTC
Cuts at: 3912 6695 3912
Size: 2783 5783
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6374 276
Size: 53 83 186 936 4840 2468
Fragments arranged by size:
4840 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI