Restriction map for
pFN21AB7001
(Circular) (Six-base) MAPSORT of: pFN21AB7001.seq Check: 196 from: 1 to: 8626
pFN21AB7001 8626 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2833 3758 4663 2833
Size: 925 905 6796
Fragments arranged by size:
6796 925 905
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4709 6436 278
Size: 53 83 186 936 3173 1727 2468
Fragments arranged by size:
3173 2468 1727 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2517 5969 1937
Size: 580 3452 4594
Fragments arranged by size:
4594 3452 580
Acc65I G'GTAC_C
Cuts at: 5947 5947
Size: 8626
AclI AA'CG_TT
Cuts at: 1563 6753 7126 1563
Size: 5190 373 3063
Fragments arranged by size:
5190 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 4527 5481 5555 5851 6689 8073 8341
Size: 1013 1882 954 74 296 838 1384 268
Cuts at: 8341 1632
Size: 1917
Fragments arranged by size:
1917 1882 1384 1013 954 838 296 268
74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8429
AflIII A'CryG_T
Cuts at: 1203 3310 5037 7525 8502 1203
Size: 2107 1727 2488 977 1327
Fragments arranged by size:
2488 2107 1727 1327 977
AgeI A'CCGG_T
Cuts at: 7457 7457
Size: 8626
AhdI GACnn_n'nnGTC
Cuts at: 7355 7355
Size: 8626
AleI CACnn'nnGTG
Cuts at: 1166 3443 1166
Size: 2277 6349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2783 3155 3461 1184
Size: 1599 372 306 6349
Fragments arranged by size:
6349 1599 372 306
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5384 5416 5384
Size: 32 8594
AlwNI CAG_nnn'CTG
Cuts at: 2382 4905 6369 7941 2382
Size: 2523 1464 1572 3067
Fragments arranged by size:
3067 2523 1572 1464
ApaI G_GGCC'C
Cuts at: 4983 4983
Size: 8626
ApaLI G'TGCA_C
Cuts at: 2420 6683 7839 2420
Size: 4263 1156 3207
Fragments arranged by size:
4263 3207 1156
ApoI r'AATT_y
Cuts at: 1493 2937 3610 4436 5851 5935 6161 6313 8422
Size: 1444 673 826 1415 84 226 152 2109
Cuts at: 8422 1493
Size: 1697
Fragments arranged by size:
2109 1697 1444 1415 826 673 226 152
84
AseI AT'TA_AT
Cuts at: 160 1032 7180 160
Size: 872 6148 1606
Fragments arranged by size:
6148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8626
AvaI C'yCGr_G
Cuts at: 1870 1943 3244 3927 5951 1870
Size: 73 1301 683 2024 4545
Fragments arranged by size:
4545 2024 1301 683 73
AvrII C'CTAG_G
Cuts at: 4786 4786
Size: 8626
BamHI G'GATC_C
Cuts at: 5956 6323 5956
Size: 367 8259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3152 3483 3743 3792 4125
Size: 325 217 627 1365 331 260 49 333
Cuts at: 4125 5152 5947 7402 618
Size: 1027 795 1455 1842
Fragments arranged by size:
1842 1455 1365 1027 795 627 333 331
325 260 217 49
BanII G_rGCy'C
Cuts at: 1391 2452 3174 3245 4983 5945 1391
Size: 1061 722 71 1738 962 4072
Fragments arranged by size:
4072 1738 1061 962 722 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2379 5496 5678 928
Size: 971 480 3117 182 3876
Fragments arranged by size:
3876 3117 971 480 182
BbvCI CC'TCA_GC
Cuts at: 5086 5086
Size: 8626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2491 2525 2882 2916 5795 5829 6817
Size: 34 554 34 357 34 2879 34 988
Cuts at: 6817 6851 1903
Size: 34 3678
Fragments arranged by size:
3678 2879 988 554 357 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6518 7728 6518
Size: 1210 7416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8626
BfrBI ATG'CAT
Cuts at: 2991 4794 5499 2991
Size: 1803 705 6118
Fragments arranged by size:
6118 1803 705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7237 136
Size: 107 122 71 631 605 5565 1525
Fragments arranged by size:
5565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3054 3888 8621 3054
Size: 834 4733 3059
Fragments arranged by size:
4733 3059 834
BlpI GC'TnA_GC
Cuts at: 2756 3260 6043 2756
Size: 504 2783 5339
Fragments arranged by size:
5339 2783 504
Bme1580I G_kGCm'C
Cuts at: 1790 2424 2472 3108 3488 4983 6687 7843 1790
Size: 634 48 636 380 1495 1704 1156 2573
Fragments arranged by size:
2573 1704 1495 1156 636 634 380 48
BmgBI CAC'GTC
Cuts at: 1318 5025 1318
Size: 3707 4919
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2317 2481 3688
Size: 416 53 83 399 24 900 164 1207
Cuts at: 3688 3857 4144 5151 6357 7315 442
Size: 169 287 1007 1206 958 1753
Fragments arranged by size:
1753 1207 1206 1007 958 900 416 399
287 169 164 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2203 2222 2687 3109 3287 3414 3842 4019
Size: 964 19 465 422 178 127 428 177
Cuts at: 4019 4878 7286 1239
Size: 859 2408 2579
Fragments arranged by size:
2579 2408 964 859 465 428 422 178
177 127 19
Bpu10I CC'TnA_GC
Cuts at: 5086 5086
Size: 8626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4640 6751 7616 7914 4640
Size: 2111 865 298 5352
Fragments arranged by size:
5352 2111 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2676 4655 7289 882
Size: 617 1177 1979 2634 2219
Fragments arranged by size:
2634 2219 1979 1177 617
BsaAI yAC'GTr
Cuts at: 493 1204 6417 493
Size: 711 5213 2702
Fragments arranged by size:
5213 2702 711
BsaBI GATnn'nnATC
Cuts at: 4295 5753 8620 4295
Size: 1458 2867 4301
Fragments arranged by size:
4301 2867 1458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4706 6433 6815 7450
Size: 53 83 186 936 3173 1727 382 635
Cuts at: 7450 275
Size: 1451
Fragments arranged by size:
3173 1727 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4481 5389 7059 7457 7731 7878 8289 4481
Size: 908 1670 398 274 147 411 4818
Fragments arranged by size:
4818 1670 908 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 2637 2667 4327
Size: 30 165 30 817 30 387 30 1660
Cuts at: 4327 4357 1178
Size: 30 5447
Fragments arranged by size:
5447 1660 817 387 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4727 1186
Size: 3541 5085
BseYI C'CCAG_C
Cuts at: 2167 2209 7829 8325 2167
Size: 42 5620 496 2468
Fragments arranged by size:
5620 2468 496 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3467 3923 1163
Size: 573 1731 456 5866
Fragments arranged by size:
5866 1731 573 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6076 6837 7865 1540
Size: 275 186 4075 761 1028 2301
Fragments arranged by size:
4075 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2424 4026 4697 6687 6772 7843 2424
Size: 1602 671 1990 85 1071 3207
Fragments arranged by size:
3207 1990 1602 1071 671 85
BsiWI C'GTAC_G
Cuts at: 5816 5816
Size: 8626
BsmI GAATG_Cn'
Cuts at: 2410 4794 5635 5647 6137 6230 2410
Size: 2384 841 12 490 93 4806
Fragments arranged by size:
4806 2384 841 490 93 12
BsmBI CGTCTCn'nnnn_
Cuts at: 2888 3034 4390 4697 2888
Size: 146 1356 307 6817
Fragments arranged by size:
6817 1356 307 146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2424 2452 2472 3108 3174 3245 3488
Size: 399 634 28 20 636 66 71 243
Cuts at: 3488 4026 4697 4983 5945 6687 6772 7843 1391
Size: 538 671 286 962 742 85 1071 2174
Fragments arranged by size:
2174 1071 962 742 671 636 634 538
399 286 243 85 71 66 28 20
BspEI T'CCGG_A
Cuts at: 4481 5389 8289 4481
Size: 908 2900 4818
Fragments arranged by size:
4818 2900 908
BspHI T'CATG_A
Cuts at: 5778 6515 8245 5778
Size: 737 1730 6159
Fragments arranged by size:
6159 1730 737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2833 3758 4663 5981 844
Size: 1989 925 905 1318 3489
Fragments arranged by size:
3489 1989 1318 925 905
BsrBI CCG'CTC
Cuts at: 1394 1823 2830 5683 6513 1394
Size: 429 1007 2853 830 3507
Fragments arranged by size:
3507 2853 1007 830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3076 5124 7121 7295 65
Size: 1183 1828 2048 1997 174 1396
Fragments arranged by size:
2048 1997 1828 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3809 7270 7457 1700
Size: 110 1999 3461 187 2869
Fragments arranged by size:
3461 2869 1999 187 110
BsrGI T'GTAC_A
Cuts at: 96 2574 3786 96
Size: 2478 1212 4936
Fragments arranged by size:
4936 2478 1212
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8626
BssSI C'ACGA_G
Cuts at: 3287 6686 7698 3287
Size: 3399 1012 4215
Fragments arranged by size:
4215 3399 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4905 794
Size: 4111 4515
BstBI TT'CG_AA
Cuts at: 3193 5707 7430 3193
Size: 2514 1723 4389
Fragments arranged by size:
4389 2514 1723
BstEII G'GTnAC_C
Cuts at: 1184 4361 1184
Size: 3177 5449
BstXI CCAn_nnnn'nTGG
Cuts at: 3167 3970 4098 5353 3167
Size: 803 128 1255 6440
Fragments arranged by size:
6440 1255 803 128
BstYI r'GATC_y
Cuts at: 1972 3054 3888 5659 5956 6323 6708 6725 8177
Size: 1082 834 1771 297 367 385 17 1452
Cuts at: 8177 8263 8275 8621 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1771 1452 1082 834 385 367 346
297 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 2662 2983 3963 5481 5578
Size: 552 939 14 644 321 980 1518 97
Cuts at: 5578 5640 513
Size: 62 3499
Fragments arranged by size:
3499 1518 980 939 644 552 321 97
62 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7887
Fragments arranged by size:
7887 683 56
BtsI GCAGTG_nn'
Cuts at: 2280 3730 4048 4169 4882 5640 5748 6142 6936
Size: 1450 318 121 713 758 108 394 794
Cuts at: 6936 6956 2280
Size: 20 3950
Fragments arranged by size:
3950 1450 794 758 713 394 318 121
108 20
ClaI AT'CG_AT
Cuts at: 1554 4563 1554
Size: 3009 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8591
DraI TTT'AAA
Cuts at: 2970 3516 4465 5930 2970
Size: 546 949 1465 5666
Fragments arranged by size:
5666 1465 949 546
DraIII CAC_nnn'GTG
Cuts at: 2443 2443
Size: 8626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7633 809
Size: 507 6317 1802
Fragments arranged by size:
6317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2557 4879
Size: 54 1425 50 275 18 447 280 2322
Cuts at: 4879 6073 6962 8591 8
Size: 1194 889 1629 43
Fragments arranged by size:
2322 1629 1425 1194 889 447 280 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6073 1537
Size: 275 4261 4090
Fragments arranged by size:
4261 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4614 4830 5329 6556 1353
Size: 3261 216 499 1227 3423
Fragments arranged by size:
3423 3261 1227 499 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7213 7587 7733 387
Size: 1064 138 5624 374 146 1280
Fragments arranged by size:
5624 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2203 2222 2645 2687 3109 3287 3414
Size: 393 571 19 423 42 422 178 127
Cuts at: 3414 3842 4019 4527 4878 5481 5555 5851 6689
Size: 428 177 508 351 603 74 296 838
Cuts at: 6689 7286 8073 8341 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 838 787 603 597 571 508 428
423 422 393 351 296 268 178 177
127 74 42 19
EcoNI CCTnn'n_nnAGG
Cuts at: 4974 5249 4974
Size: 275 8351
EcoO109I rG'GnC_Cy
Cuts at: 2653 2961 3577 3851 4059 4979 4980 5583 2653
Size: 308 616 274 208 920 1 603 5696
Fragments arranged by size:
5696 920 616 603 308 274 208 1
EcoRI G'AATT_C
Cuts at: 2937 4436 5935 6313 2937
Size: 1499 1499 378 5250
Fragments arranged by size:
5250 1499 1499 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2995 4146 4557 2995
Size: 1151 411 7064
Fragments arranged by size:
7064 1151 411
FspI TGC'GCA
Cuts at: 7132 7132
Size: 8626
HaeII r_GCGC'y
Cuts at: 1678 3942 7773 1678
Size: 2264 3831 2531
Fragments arranged by size:
3831 2531 2264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2499 2531 2587 2603 2619
Size: 32 135 32 927 32 56 16 16
Cuts at: 2619 2635 2637 2669 2673 2705 3158 3190 4327
Size: 16 2 32 4 32 453 32 1137
Cuts at: 4327 4359 7267 7299 7341 7373 1373
Size: 32 2908 32 42 32 2626
Fragments arranged by size:
2908 2626 1137 927 453 135 56 42
32 32 32 32 32 32 32 32
32 16 16 16 4 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3121 5970 6216 669
Size: 988 281 1183 2849 246 3079
Fragments arranged by size:
3079 2849 1183 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5224 748
Size: 4476 4150
HpaI GTT'AAC
Cuts at: 6216 6216
Size: 8626
KpnI G_GTAC'C
Cuts at: 5951 5951
Size: 8626
MfeI C'AATT_G
Cuts at: 4213 6225 4213
Size: 2012 6614
MluI A'CGCG_T
Cuts at: 3310 8502 3310
Size: 5192 3434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2170 4689 7740 7924 1305
Size: 865 2519 3051 184 2007
Fragments arranged by size:
3051 2519 2007 865 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 6357
Fragments arranged by size:
6357 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3443 4812 5351 6584 6943 7102 518
Size: 648 2277 1369 539 1233 359 159 2042
Fragments arranged by size:
2277 2042 1369 1233 648 539 359 159
MspA1I CmG'CkG
Cuts at: 1606 3129 3840 3997 4735 5601 6042 6719 7867
Size: 1523 711 157 738 866 441 677 1148
Cuts at: 7867 8112 1606
Size: 245 2120
Fragments arranged by size:
2120 1523 1148 866 738 711 677 441
245 157
NaeI GCC'GGC
Cuts at: 3811 3811
Size: 8626
NcoI C'CATG_G
Cuts at: 513 1065 2004 2662 2983 3963 5481 5578 513
Size: 552 939 658 321 980 1518 97 3561
Fragments arranged by size:
3561 1518 980 939 658 552 321 97
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8626
NgoMIV G'CCGG_C
Cuts at: 3809 3809
Size: 8626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8626
NotI GC'GGCC_GC
Cuts at: 6073 6073
Size: 8626
NsiI A_TGCA'T
Cuts at: 2993 4796 5501 2993
Size: 1803 705 6118
Fragments arranged by size:
6118 1803 705
NspI r_CATG'y
Cuts at: 1132 1229 2641 5041 5503 5984 7529 1132
Size: 97 1412 2400 462 481 1545 2229
Fragments arranged by size:
2400 2229 1545 1412 481 462 97
PasI CC'CwG_GG
Cuts at: 1348 2782 3604 1348
Size: 1434 822 6370
Fragments arranged by size:
6370 1434 822
PciI A'CATG_T
Cuts at: 5037 7525 5037
Size: 2488 6138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8626
PfoI T'CCnGG_A
Cuts at: 2793 3698 2793
Size: 905 7721
PmeI GTTT'AAAC
Cuts at: 5930 5930
Size: 8626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 6695 6727 8208 8240 4370
Size: 32 2293 32 1481 32 4756
Fragments arranged by size:
4756 2293 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2653 2961 3577 3851 4059 5583 2653
Size: 308 616 274 208 1524 5696
Fragments arranged by size:
5696 1524 616 308 274 208
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8626
PsiI TTA'TAA
Cuts at: 5379 5411 6196 5379
Size: 32 785 7809
Fragments arranged by size:
7809 785 32
PspOMI G'GGCC_C
Cuts at: 4979 4979
Size: 8626
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4078 4110 4078
Size: 32 8594
PstI C_TGCA'G
Cuts at: 830 3725 4055 4674 4734 4889 5978 830
Size: 2895 330 619 60 155 1089 3478
Fragments arranged by size:
3478 2895 1089 619 330 155 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8626
PvuII CAG'CTG
Cuts at: 3997 4735 5601 3997
Size: 738 866 7022
Fragments arranged by size:
7022 866 738
SalI G'TCGA_C
Cuts at: 1936 5968 1936
Size: 4032 4594
SbfI CC_TGCA'GG
Cuts at: 4674 5978 4674
Size: 1304 7322
ScaI AGT'ACT
Cuts at: 2602 6874 8586 2602
Size: 4272 1712 2642
Fragments arranged by size:
4272 2642 1712
SexAI A'CCwGG_T
Cuts at: 4097 4097
Size: 8626
SfcI C'TryA_G
Cuts at: 826 1045 3721 4051 4670 4730 4885 5974 7109
Size: 219 2676 330 619 60 155 1089 1135
Cuts at: 7109 7790 7981 826
Size: 681 191 1471
Fragments arranged by size:
2676 1471 1135 1089 681 619 330 219
191 155 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8626
SmaI CCC'GGG
Cuts at: 1872 5953 1872
Size: 4081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4655 6730 7631 7893 820
Size: 197 926 2712 2075 901 262 1553
Fragments arranged by size:
2712 2075 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6417 493
Size: 5924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8626
SphI G_CATG'C
Cuts at: 1132 2641 5984 1132
Size: 1509 3343 3774
Fragments arranged by size:
3774 3343 1509
SspI AAT'ATT
Cuts at: 5 52 5918 6550 5
Size: 47 5866 632 2081
Fragments arranged by size:
5866 2081 632 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8626
StyI C'CwwG_G
Cuts at: 513 1065 2004 2662 2694 2886 2983 3535 3573
Size: 552 939 658 32 192 97 552 38
Cuts at: 3573 3963 3969 4688 4786 5115 5424 5481 5578
Size: 390 6 719 98 329 309 57 97
Cuts at: 5578 6065 513
Size: 487 3074
Fragments arranged by size:
3074 939 719 658 552 552 487 390
329 309 192 98 97 97 57 38
32 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2299 2726 2806 3433 4380 5170 6653
Size: 1255 719 427 80 627 947 790 1483
Cuts at: 6653 6670 6823 7008 7453 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1483 1255 947 790 719 627 445
427 185 153 80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2361 2574 2600
Size: 274 80 33 51 1443 384 213 26
Cuts at: 2600 3786 3864 5514 6872 8584 96
Size: 1186 78 1650 1358 1712 138
Fragments arranged by size:
1712 1650 1443 1358 1186 384 274 213
138 80 78 51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2473 2505 2862 2894 3601 3633 8586 8618 2473
Size: 32 357 32 707 32 4953 32 2481
Fragments arranged by size:
4953 2481 707 357 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8626
XbaI T'CTAG_A
Cuts at: 5962 5962
Size: 8626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3438 4098 3438
Size: 660 7966
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8626
XmaI C'CCGG_G
Cuts at: 1870 5951 1870
Size: 4081 4545
XmnI GAAnn'nnTTC
Cuts at: 2978 4781 5713 6755 2978
Size: 1803 932 1042 4849
Fragments arranged by size:
4849 1803 1042 932
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4707 6434 276
Size: 53 83 186 936 3173 1727 2468
Fragments arranged by size:
3173 2468 1727 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BplI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NarI NruI PacI PmlI
PspXI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI