(Circular) (Six-base) MAPSORT of: pFN21AB6996.seq Check: 2665 from: 1 to: 5467 pFN21AB6996 5467 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3277 278 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2810 1937 Size: 873 4594 Acc65I G'GTAC_C Cuts at: 2788 2788 Size: 5467 AclI AA'CG_TT Cuts at: 1563 3594 3967 1563 Size: 2031 373 3063 Fragments arranged by size: 3063 2031 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2460 2514 3530 4914 5182 1632 Size: 828 54 1016 1384 268 1917 Fragments arranged by size: 1917 1384 1016 828 268 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5270 AflIII A'CryG_T Cuts at: 1203 2178 4366 5343 1203 Size: 975 2188 977 1327 Fragments arranged by size: 2188 1327 977 975 AgeI A'CCGG_T Cuts at: 4298 4298 Size: 5467 AhdI GACnn_n'nnGTC Cuts at: 4196 4196 Size: 5467 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5467 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5467 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2226 2258 2226 Size: 32 5435 AlwNI CAG_nnn'CTG Cuts at: 2113 2492 3210 4782 2113 Size: 379 718 1572 2798 Fragments arranged by size: 2798 1572 718 379 ApaLI G'TGCA_C Cuts at: 3524 4680 3524 Size: 1156 4311 ApoI r'AATT_y Cuts at: 1493 2395 2673 2776 3002 3154 5263 1493 Size: 902 278 103 226 152 2109 1697 Fragments arranged by size: 2109 1697 902 278 226 152 103 AseI AT'TA_AT Cuts at: 160 1032 4021 160 Size: 872 2989 1606 Fragments arranged by size: 2989 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 AvaI C'yCGr_G Cuts at: 1870 1943 2792 1870 Size: 73 849 4545 Fragments arranged by size: 4545 849 73 BamHI G'GATC_C Cuts at: 2797 3164 2797 Size: 367 5100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2788 4243 618 Size: 325 217 627 1001 1455 1842 Fragments arranged by size: 1842 1455 1001 627 325 217 BanII G_rGCy'C Cuts at: 1391 2786 1391 Size: 1395 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2332 928 Size: 971 433 4063 Fragments arranged by size: 4063 971 433 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3658 3692 1903 Size: 34 1721 34 3678 Fragments arranged by size: 3678 1721 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3359 4569 3359 Size: 1210 4257 BclI T'GATC_A Cuts at: 1548 1548 Size: 5467 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4078 136 Size: 107 122 71 631 605 337 2069 1525 Fragments arranged by size: 2069 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5462 5462 Size: 5467 BlpI GC'TnA_GC Cuts at: 2884 2884 Size: 5467 Bme1580I G_kGCm'C Cuts at: 1790 3528 4684 1790 Size: 1738 1156 2573 Fragments arranged by size: 2573 1738 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5467 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2162 3198 4156 Size: 416 53 83 399 24 745 1036 958 Cuts at: 4156 442 Size: 1753 Fragments arranged by size: 1753 1036 958 745 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2606 4127 1239 Size: 1367 1521 2579 Fragments arranged by size: 2579 1521 1367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 3592 4457 4755 2267 Size: 1325 865 298 2979 Fragments arranged by size: 2979 1325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4130 882 Size: 617 2631 2219 Fragments arranged by size: 2631 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3258 493 Size: 711 2054 2702 Fragments arranged by size: 2702 2054 711 BsaBI GATnn'nnATC Cuts at: 5461 5461 Size: 5467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3274 3656 4291 275 Size: 53 83 186 936 1741 382 635 1451 Fragments arranged by size: 1741 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2701 3900 4298 4572 4719 5130 2701 Size: 1199 398 274 147 411 3038 Fragments arranged by size: 3038 1199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2562 2592 1178 Size: 30 165 30 1159 30 4053 Fragments arranged by size: 4053 1159 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5467 BseYI C'CCAG_C Cuts at: 2028 2275 4670 5166 2028 Size: 247 2395 496 2329 Fragments arranged by size: 2395 2329 496 247 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2579 1163 Size: 573 843 4051 Fragments arranged by size: 4051 843 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2917 3678 4706 1540 Size: 275 186 916 761 1028 2301 Fragments arranged by size: 2301 1028 916 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3528 3613 4684 3528 Size: 85 1071 4311 Fragments arranged by size: 4311 1071 85 BsmI GAATG_Cn' Cuts at: 2978 3071 2978 Size: 93 5374 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2786 3528 3613 4684 1391 Size: 399 996 742 85 1071 2174 Fragments arranged by size: 2174 1071 996 742 399 85 BspEI T'CCGG_A Cuts at: 2701 5130 2701 Size: 2429 3038 BspHI T'CATG_A Cuts at: 3356 5086 3356 Size: 1730 3737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2158 2822 844 Size: 1314 664 3489 Fragments arranged by size: 3489 1314 664 BsrBI CCG'CTC Cuts at: 1394 1823 3354 1394 Size: 429 1531 3507 Fragments arranged by size: 3507 1531 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 3962 4136 65 Size: 1183 1275 1439 174 1396 Fragments arranged by size: 1439 1396 1275 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4111 4298 1700 Size: 110 2301 187 2869 Fragments arranged by size: 2869 2301 187 110 BsrGI T'GTAC_A Cuts at: 96 2125 2608 96 Size: 2029 483 2955 Fragments arranged by size: 2955 2029 483 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5467 BssSI C'ACGA_G Cuts at: 3527 4539 3527 Size: 1012 4455 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5467 BstBI TT'CG_AA Cuts at: 4271 4271 Size: 5467 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5467 BstYI r'GATC_y Cuts at: 1972 2489 2797 3164 3549 3566 5018 5104 5116 Size: 517 308 367 385 17 1452 86 12 Cuts at: 5116 5462 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 517 385 367 346 308 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2259 513 Size: 552 939 255 3721 Fragments arranged by size: 3721 939 552 255 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4728 Fragments arranged by size: 4728 683 56 BtsI GCAGTG_nn' Cuts at: 2983 3777 3797 2983 Size: 794 20 4653 Fragments arranged by size: 4653 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5467 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5432 DraI TTT'AAA Cuts at: 2771 2771 Size: 5467 DraIII CAC_nnn'GTG Cuts at: 2170 2170 Size: 5467 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4474 809 Size: 507 3158 1802 Fragments arranged by size: 3158 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2549 2914 Size: 54 1425 50 275 18 186 533 365 Cuts at: 2914 3803 5432 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 533 365 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2914 1537 Size: 275 1102 4090 Fragments arranged by size: 4090 1102 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3397 1353 Size: 2044 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4054 4428 4574 387 Size: 1064 138 2465 374 146 1280 Fragments arranged by size: 2465 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2460 2514 2606 3530 4127 4914 5182 Size: 393 828 54 92 924 597 787 268 Cuts at: 5182 1239 Size: 1524 Fragments arranged by size: 1524 924 828 787 597 393 268 92 54 EcoRI G'AATT_C Cuts at: 2673 2776 3154 2673 Size: 103 378 4986 Fragments arranged by size: 4986 378 103 FspI TGC'GCA Cuts at: 2060 3973 2060 Size: 1913 3554 HaeII r_GCGC'y Cuts at: 1678 4614 1678 Size: 2936 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2723 2755 4108 4140 4182 Size: 32 135 32 1151 32 1353 32 42 Cuts at: 4182 4214 1373 Size: 32 2626 Fragments arranged by size: 2626 1353 1151 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2811 3057 669 Size: 988 281 873 246 3079 Fragments arranged by size: 3079 988 873 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5467 HpaI GTT'AAC Cuts at: 3057 3057 Size: 5467 KpnI G_GTAC'C Cuts at: 2792 2792 Size: 5467 MfeI C'AATT_G Cuts at: 3066 3066 Size: 5467 MluI A'CGCG_T Cuts at: 5343 5343 Size: 5467 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4581 4765 1305 Size: 3276 184 2007 Fragments arranged by size: 3276 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3645 Fragments arranged by size: 3645 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3425 3784 3943 518 Size: 648 2259 359 159 2042 Fragments arranged by size: 2259 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2279 2614 2640 2883 3560 4708 4953 1606 Size: 673 335 26 243 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 673 335 245 243 26 NcoI C'CATG_G Cuts at: 513 1065 2004 2259 513 Size: 552 939 255 3721 Fragments arranged by size: 3721 939 552 255 NdeI CA'TA_TG Cuts at: 387 2076 387 Size: 1689 3778 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5467 NotI GC'GGCC_GC Cuts at: 2914 2914 Size: 5467 NspI r_CATG'y Cuts at: 1132 1229 2182 2686 2825 4370 1132 Size: 97 953 504 139 1545 2229 Fragments arranged by size: 2229 1545 953 504 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5467 PciI A'CATG_T Cuts at: 2178 4366 2178 Size: 2188 3279 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5467 PmeI GTTT'AAAC Cuts at: 2771 2771 Size: 5467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3536 3568 5049 5081 3536 Size: 32 1481 32 3922 Fragments arranged by size: 3922 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5467 PsiI TTA'TAA Cuts at: 3037 3037 Size: 5467 PstI C_TGCA'G Cuts at: 830 2155 2284 2691 2819 830 Size: 1325 129 407 128 3478 Fragments arranged by size: 3478 1325 407 129 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5467 PvuII CAG'CTG Cuts at: 2279 2614 2640 2279 Size: 335 26 5106 Fragments arranged by size: 5106 335 26 SalI G'TCGA_C Cuts at: 1936 2809 1936 Size: 873 4594 SbfI CC_TGCA'GG Cuts at: 2819 2819 Size: 5467 ScaI AGT'ACT Cuts at: 3715 5427 3715 Size: 1712 3755 SfcI C'TryA_G Cuts at: 826 1045 2151 2280 2687 2815 3950 4631 4822 Size: 219 1106 129 407 128 1135 681 191 Cuts at: 4822 826 Size: 1471 Fragments arranged by size: 1471 1135 1106 681 407 219 191 129 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5467 SmaI CCC'GGG Cuts at: 1872 2794 1872 Size: 922 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2246 3571 4472 4734 820 Size: 197 926 303 1325 901 262 1553 Fragments arranged by size: 1553 1325 926 901 303 262 197 SnaBI TAC'GTA Cuts at: 493 3258 493 Size: 2765 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5467 SphI G_CATG'C Cuts at: 1132 2825 1132 Size: 1693 3774 SspI AAT'ATT Cuts at: 5 52 3391 5 Size: 47 3339 2081 Fragments arranged by size: 3339 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5467 StyI C'CwwG_G Cuts at: 513 1065 2004 2259 2906 513 Size: 552 939 255 647 3074 Fragments arranged by size: 3074 939 647 552 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2181 3494 3511 3664 3849 4294 325 Size: 1255 601 1313 17 153 185 445 1498 Fragments arranged by size: 1498 1313 1255 601 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2125 2608 3713 Size: 274 80 33 51 1443 148 483 1105 Cuts at: 3713 5425 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1105 483 274 148 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5427 5459 5427 Size: 32 5435 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5467 XbaI T'CTAG_A Cuts at: 2803 2803 Size: 5467 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5467 XmaI C'CCGG_G Cuts at: 1870 2792 1870 Size: 922 4545 XmnI GAAnn'nnTTC Cuts at: 3596 3596 Size: 5467 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3275 276 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI