Restriction map for
pFN21AB6994
(Circular) (Six-base) MAPSORT of: pFN21AB6994.seq Check: 4079 from: 1 to: 5464
pFN21AB6994 5464 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2053
Size: 5464
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2325 3527 4911 5179 1632
Size: 693 1202 1384 268 1917
Fragments arranged by size:
1917 1384 1202 693 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 2614 3207 4779 2614
Size: 593 1572 3299
Fragments arranged by size:
3299 1572 593
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2285 2398 2617 2715 2773 2999 3151 5260
Size: 792 113 219 98 58 226 152 2109
Cuts at: 5260 1493
Size: 1697
Fragments arranged by size:
2109 1697 792 226 219 152 113 98
58
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 1870
Size: 73 846 4545
Fragments arranged by size:
4545 846 73
BamHI G'GATC_C
Cuts at: 2548 2794 3161 2548
Size: 246 367 4851
Fragments arranged by size:
4851 367 246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2785 4240 618
Size: 325 217 627 998 1455 1842
Fragments arranged by size:
1842 1455 998 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2783 1391
Size: 1392 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2499 928
Size: 971 600 3893
Fragments arranged by size:
3893 971 600
BbvCI CC'TCA_GC
Cuts at: 2240 2240
Size: 5464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 2314 1548
Size: 766 4698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4075 136
Size: 107 122 71 631 605 2403 1525
Fragments arranged by size:
2403 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2562 5459 2562
Size: 2897 2567
BlpI GC'TnA_GC
Cuts at: 2102 2881 2102
Size: 779 4685
Bme1580I G_kGCm'C
Cuts at: 1790 3525 4681 1790
Size: 1735 1156 2573
Fragments arranged by size:
2573 1735 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3195 4153 442
Size: 416 53 83 399 24 1778 958 1753
Fragments arranged by size:
1778 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2178 2246 2428 4124 1239
Size: 939 68 182 1696 2579
Fragments arranged by size:
2579 1696 939 182 68
Bpu10I CC'TnA_GC
Cuts at: 2240 2240
Size: 5464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2402 4127 882
Size: 617 903 1725 2219
Fragments arranged by size:
2219 1725 903 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3271 3653 4288 275
Size: 53 83 186 936 1738 382 635 1451
Fragments arranged by size:
1738 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2662 3897 4295 4569 4716 5127 2662
Size: 1235 398 274 147 411 2999
Fragments arranged by size:
2999 1235 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5239
Fragments arranged by size:
5239 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 4533
BseYI C'CCAG_C
Cuts at: 2028 4667 5163 2028
Size: 2639 496 2329
Fragments arranged by size:
2639 2329 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4891
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3525 3610 4681 3525
Size: 85 1071 4308
Fragments arranged by size:
4308 1071 85
BsmI GAATG_Cn'
Cuts at: 2536 2761 2975 3068 2536
Size: 225 214 93 4932
Fragments arranged by size:
4932 225 214 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2783 3525 3610 4681 1391
Size: 399 993 742 85 1071 2174
Fragments arranged by size:
2174 1071 993 742 399 85
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 2317 3353 5083 2317
Size: 1036 1730 2698
Fragments arranged by size:
2698 1730 1036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 2244 2819 844
Size: 1209 191 575 3489
Fragments arranged by size:
3489 1209 575 191
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3959 4133 65
Size: 1183 2711 174 1396
Fragments arranged by size:
2711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 4108 4295 1700
Size: 110 287 2011 187 2869
Fragments arranged by size:
2869 2011 287 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 2117 1925
Size: 192 5272
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5464
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstXI CCAn_nnnn'nTGG
Cuts at: 2350 2350
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2548 2562 2794 3161 3546 3563 5015 5101
Size: 576 14 232 367 385 17 1452 86
Cuts at: 5101 5113 5459 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 576 385 367 346 232 86
17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2049 2184 513
Size: 552 984 135 3793
Fragments arranged by size:
3793 984 552 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2099 2730 2980 3774 3794 2099
Size: 631 250 794 20 3769
Fragments arranged by size:
3769 794 631 250 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2169 2187
Size: 54 1425 50 275 18 222 117 18
Cuts at: 2187 2911 3800 5429 8
Size: 724 889 1629 43
Fragments arranged by size:
1629 1425 889 724 275 222 117 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2301 3394 1353
Size: 948 1093 3423
Fragments arranged by size:
3423 1093 948
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2246 2325 2428 3527 4124 4911
Size: 393 546 68 79 103 1099 597 787
Cuts at: 4911 5179 1239
Size: 268 1524
Fragments arranged by size:
1524 1099 787 597 546 393 268 103
79 68
EcoO109I rG'GnC_Cy
Cuts at: 2611 2611
Size: 5464
EcoRI G'AATT_C
Cuts at: 2715 2773 3151 2715
Size: 58 378 5028
Fragments arranged by size:
5028 378 58
EcoRV GAT'ATC
Cuts at: 2326 2326
Size: 5464
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 4611 1678
Size: 2933 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2734 2766 4105 4137 4179
Size: 32 135 32 1162 32 1339 32 42
Cuts at: 4179 4211 1373
Size: 32 2626
Fragments arranged by size:
2626 1339 1162 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 2537 748
Size: 1789 3675
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4578 4762 1305
Size: 3273 184 2007
Fragments arranged by size:
3273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2171 2189 10
Size: 54 1425 343 339 18 3285
Fragments arranged by size:
3285 1425 343 339 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 3422 3781 3940 518
Size: 648 2256 359 159 2042
Fragments arranged by size:
2256 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2072 2880 3557 4705 4950 1606
Size: 445 21 808 677 1148 245 2120
Fragments arranged by size:
2120 1148 808 677 445 245 21
NaeI GCC'GGC
Cuts at: 2099 2099
Size: 5464
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4912
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NgoMIV G'CCGG_C
Cuts at: 2097 2097
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2110 2260 2584 2822 4367 1132
Size: 97 881 150 324 238 1545 2229
Fragments arranged by size:
2229 1545 881 324 238 150 97
PasI CC'CwG_GG
Cuts at: 1348 2015 1348
Size: 667 4797
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PfoI T'CCnGG_A
Cuts at: 2151 2151
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 2673 3034 2673
Size: 361 5103
PstI C_TGCA'G
Cuts at: 830 2094 2816 830
Size: 1264 722 3478
Fragments arranged by size:
3478 1264 722
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
SacII CC_GC'GG
Cuts at: 2052 2052
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SexAI A'CCwGG_T
Cuts at: 2508 2508
Size: 5464
SfcI C'TryA_G
Cuts at: 826 1045 2090 2812 3947 4628 4819 826
Size: 219 1045 722 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 722 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2791 1872
Size: 919 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3568 4469 4731 820
Size: 197 926 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 2357 1517
Size: 840 4624
StyI C'CwwG_G
Cuts at: 513 1065 2903 513
Size: 552 1838 3074
Fragments arranged by size:
3074 1838 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3491 3508 3661 3846 4291 325
Size: 1255 1911 17 153 185 445 1498
Fragments arranged by size:
1911 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3710 5422 96
Size: 274 80 33 51 1443 1733 1712 138
Fragments arranged by size:
1733 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2281 2569 2800 2281
Size: 288 231 4945
Fragments arranged by size:
4945 288 231
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2789 1870
Size: 919 4545
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SfiI SfoI SrfI SwaI XcmI