Restriction map for
pFN21AB6984
(Circular) (Six-base) MAPSORT of: pFN21AB6984.seq Check: 2864 from: 1 to: 5446
pFN21AB6984 5446 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3256 278
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2688 2700 2789 1937
Size: 751 12 89 4594
Fragments arranged by size:
4594 751 89 12
Acc65I G'GTAC_C
Cuts at: 2767 2767
Size: 5446
AclI AA'CG_TT
Cuts at: 1563 3573 3946 1563
Size: 2010 373 3063
Fragments arranged by size:
3063 2010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3509 4893 5161 1632
Size: 1877 1384 268 1917
Fragments arranged by size:
1917 1877 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5249
AflIII A'CryG_T
Cuts at: 1203 2550 4345 5322 1203
Size: 1347 1795 977 1327
Fragments arranged by size:
1795 1347 1327 977
AgeI A'CCGG_T
Cuts at: 4277 4277
Size: 5446
AhdI GACnn_n'nnGTC
Cuts at: 4175 4175
Size: 5446
AleI CACnn'nnGTG
Cuts at: 1166 2145 1166
Size: 979 4467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2209 1184
Size: 1025 4421
AlwNI CAG_nnn'CTG
Cuts at: 2408 2485 3189 4761 2408
Size: 77 704 1572 3093
Fragments arranged by size:
3093 1572 704 77
ApaLI G'TGCA_C
Cuts at: 3503 4659 3503
Size: 1156 4290
ApoI r'AATT_y
Cuts at: 1493 2101 2128 2755 2981 3133 5242 1493
Size: 608 27 627 226 152 2109 1697
Fragments arranged by size:
2109 1697 627 608 226 152 27
AseI AT'TA_AT
Cuts at: 160 1032 4000 160
Size: 872 2968 1606
Fragments arranged by size:
2968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 1870
Size: 73 828 4545
Fragments arranged by size:
4545 828 73
BamHI G'GATC_C
Cuts at: 2776 3143 2776
Size: 367 5079
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2456 2767 4222 618
Size: 325 217 627 669 311 1455 1842
Fragments arranged by size:
1842 1455 669 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2453 2765 1391
Size: 1062 312 4072
Fragments arranged by size:
4072 1062 312
BbeI G_GCGC'C
Cuts at: 2460 2460
Size: 5446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 928
Size: 971 416 4059
Fragments arranged by size:
4059 971 416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3637 3671 1903
Size: 34 1700 34 3678
Fragments arranged by size:
3678 1700 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3338 4548 3338
Size: 1210 4236
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4057 136
Size: 107 122 71 631 605 2385 1525
Fragments arranged by size:
2385 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5441 5441
Size: 5446
BlpI GC'TnA_GC
Cuts at: 2501 2863 2501
Size: 362 5084
Bme1580I G_kGCm'C
Cuts at: 1790 2668 3507 4663 1790
Size: 878 839 1156 2573
Fragments arranged by size:
2573 1156 878 839
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5446
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2231 3177 4135
Size: 416 53 83 399 24 814 946 958
Cuts at: 4135 442
Size: 1753
Fragments arranged by size:
1753 958 946 814 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2385 2703 4106 1239
Size: 1028 118 318 1403 2579
Fragments arranged by size:
2579 1403 1028 318 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2591 3571 4436 4734 2591
Size: 980 865 298 3303
Fragments arranged by size:
3303 980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4109 882
Size: 617 2610 2219
Fragments arranged by size:
2610 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2351 3237 493
Size: 711 1147 886 2702
Fragments arranged by size:
2702 1147 886 711
BsaBI GATnn'nnATC
Cuts at: 5440 5440
Size: 5446
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2457 3253 3635 4270
Size: 53 83 186 936 924 796 382 635
Cuts at: 4270 275
Size: 1451
Fragments arranged by size:
1451 936 924 796 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3879 4277 4551 4698 5109 3879
Size: 398 274 147 411 4216
Fragments arranged by size:
4216 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2383 2413 1178
Size: 30 165 30 980 30 4211
Fragments arranged by size:
4211 980 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2335 1186
Size: 925 224 4297
Fragments arranged by size:
4297 925 224
BseYI C'CCAG_C
Cuts at: 2433 4649 5145 2433
Size: 2216 496 2734
Fragments arranged by size:
2734 2216 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2373 1163
Size: 573 637 4236
Fragments arranged by size:
4236 637 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2896 3657 4685 1540
Size: 275 186 895 761 1028 2301
Fragments arranged by size:
2301 1028 895 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 2453 3507 3592 4663 2232
Size: 221 1054 85 1071 3015
Fragments arranged by size:
3015 1071 1054 221 85
BsmI GAATG_Cn'
Cuts at: 2957 3050 2957
Size: 93 5353
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2453 2668 2765 3507 3592 4663
Size: 399 442 221 215 97 742 85 1071
Cuts at: 4663 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 442 399 221 215 97
85
BspEI T'CCGG_A
Cuts at: 5109 5109
Size: 5446
BspHI T'CATG_A
Cuts at: 3335 5065 3335
Size: 1730 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2801 844
Size: 1957 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3333 1394
Size: 429 1510 3507
Fragments arranged by size:
3507 1510 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2629 3941 4115 65
Size: 1183 1381 1312 174 1396
Fragments arranged by size:
1396 1381 1312 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4090 4277 1700
Size: 110 2280 187 2869
Fragments arranged by size:
2869 2280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5446
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5446
BssSI C'ACGA_G
Cuts at: 3506 4518 3506
Size: 1012 4434
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2418 794
Size: 1624 3822
BstBI TT'CG_AA
Cuts at: 4250 4250
Size: 5446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5446
BstXI CCAn_nnnn'nTGG
Cuts at: 2147 2147
Size: 5446
BstYI r'GATC_y
Cuts at: 1972 2776 3143 3528 3545 4997 5083 5095 5441
Size: 804 367 385 17 1452 86 12 346
Cuts at: 5441 1972
Size: 1977
Fragments arranged by size:
1977 1452 804 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4894
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4707
Fragments arranged by size:
4707 683 56
BtsI GCAGTG_nn'
Cuts at: 2191 2442 2486 2962 3756 3776 2191
Size: 251 44 476 794 20 3861
Fragments arranged by size:
3861 794 476 251 44 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5411
DraI TTT'AAA
Cuts at: 2750 2750
Size: 5446
DraIII CAC_nnn'GTG
Cuts at: 2418 2418
Size: 5446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4453 809
Size: 507 3137 1802
Fragments arranged by size:
3137 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2893 3782 5411
Size: 54 1425 50 275 18 1063 889 1629
Cuts at: 5411 8
Size: 43
Fragments arranged by size:
1629 1425 1063 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2893 1537
Size: 275 1081 4090
Fragments arranged by size:
4090 1081 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3376 1353
Size: 2023 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2276 4033 4407 4553 387
Size: 1064 138 687 1757 374 146 1280
Fragments arranged by size:
1757 1280 1064 687 374 146 138
EcoICRI GAG'CTC
Cuts at: 2451 2451
Size: 5446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2385 2703 3509 4106 4893 5161
Size: 393 635 118 318 806 597 787 268
Cuts at: 5161 1239
Size: 1524
Fragments arranged by size:
1524 806 787 635 597 393 318 268
118
EcoRI G'AATT_C
Cuts at: 2101 2128 2755 3133 2101
Size: 27 627 378 4414
Fragments arranged by size:
4414 627 378 27
FspI TGC'GCA
Cuts at: 3952 3952
Size: 5446
HaeII r_GCGC'y
Cuts at: 1678 2460 4593 1678
Size: 782 2133 2531
Fragments arranged by size:
2531 2133 782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4087 4119 4161 4193 1373
Size: 32 135 32 2515 32 42 32 2626
Fragments arranged by size:
2626 2515 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2790 3036 669
Size: 988 281 852 246 3079
Fragments arranged by size:
3079 988 852 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5446
HpaI GTT'AAC
Cuts at: 3036 3036
Size: 5446
KasI G'GCGC_C
Cuts at: 2456 2456
Size: 5446
KpnI G_GTAC'C
Cuts at: 2771 2771
Size: 5446
MfeI C'AATT_G
Cuts at: 3045 3045
Size: 5446
MluI A'CGCG_T
Cuts at: 5322 5322
Size: 5446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4560 4744 1305
Size: 3255 184 2007
Fragments arranged by size:
3255 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3624
Fragments arranged by size:
3624 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2213 3404 3763 3922 518
Size: 648 979 68 1191 359 159 2042
Fragments arranged by size:
2042 1191 979 648 359 159 68
MspA1I CmG'CkG
Cuts at: 1606 2039 2862 3539 4687 4932 1606
Size: 433 823 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 677 433 245
NarI GG'CG_CC
Cuts at: 2457 2457
Size: 5446
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4894
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5446
NotI GC'GGCC_GC
Cuts at: 2893 2893
Size: 5446
NspI r_CATG'y
Cuts at: 1132 1229 2554 2804 4349 1132
Size: 97 1325 250 1545 2229
Fragments arranged by size:
2229 1545 1325 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5446
PciI A'CATG_T
Cuts at: 2550 4345 2550
Size: 1795 3651
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2408 1108
Size: 1300 4146
PfoI T'CCnGG_A
Cuts at: 2244 2244
Size: 5446
PmeI GTTT'AAAC
Cuts at: 2750 2750
Size: 5446
PmlI CAC'GTG
Cuts at: 2351 2351
Size: 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5028 5060 3515
Size: 32 1481 32 3901
Fragments arranged by size:
3901 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5446
PsiI TTA'TAA
Cuts at: 3016 3016
Size: 5446
PstI C_TGCA'G
Cuts at: 830 2481 2798 830
Size: 1651 317 3478
Fragments arranged by size:
3478 1651 317
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5446
PvuII CAG'CTG
Cuts at: 2039 2039
Size: 5446
SacI G_AGCT'C
Cuts at: 2453 2453
Size: 5446
SalI G'TCGA_C
Cuts at: 1936 2788 1936
Size: 852 4594
SbfI CC_TGCA'GG
Cuts at: 2798 2798
Size: 5446
ScaI AGT'ACT
Cuts at: 3694 5406 3694
Size: 1712 3734
SexAI A'CCwGG_T
Cuts at: 2115 2115
Size: 5446
SfcI C'TryA_G
Cuts at: 826 1045 2477 2794 3929 4610 4801 826
Size: 219 1432 317 1135 681 191 1471
Fragments arranged by size:
1471 1432 1135 681 317 219 191
SfoI GGC'GCC
Cuts at: 2458 2458
Size: 5446
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5446
SmaI CCC'GGG
Cuts at: 1872 2773 1872
Size: 901 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2570 3550 4451 4713 820
Size: 197 926 627 980 901 262 1553
Fragments arranged by size:
1553 980 926 901 627 262 197
SnaBI TAC'GTA
Cuts at: 493 3237 493
Size: 2744 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5446
SphI G_CATG'C
Cuts at: 1132 2804 1132
Size: 1672 3774
SspI AAT'ATT
Cuts at: 5 52 3370 5
Size: 47 3318 2081
Fragments arranged by size:
3318 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5446
StyI C'CwwG_G
Cuts at: 513 1065 2635 2694 2885 513
Size: 552 1570 59 191 3074
Fragments arranged by size:
3074 1570 552 191 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2164 3473 3490 3643 3828 4273 325
Size: 1255 584 1309 17 153 185 445 1498
Fragments arranged by size:
1498 1309 1255 584 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3692 5404 96
Size: 274 80 33 51 1443 1715 1712 138
Fragments arranged by size:
1715 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 2383 2415 5406 5438 2004
Size: 32 347 32 2991 32 2012
Fragments arranged by size:
2991 2012 347 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5446
XbaI T'CTAG_A
Cuts at: 2782 2782
Size: 5446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2147 2147
Size: 5446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5446
XmaI C'CCGG_G
Cuts at: 1870 2771 1870
Size: 901 4545
XmnI GAAnn'nnTTC
Cuts at: 3575 3575
Size: 5446
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3254 276
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SfiI SrfI SwaI