Restriction map for
pFN21AB6982
(Circular) (Six-base) MAPSORT of: pFN21AB6982.seq Check: 3155 from: 1 to: 5443
pFN21AB6982 5443 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2376 2612 2786 1937
Size: 439 236 174 4594
Fragments arranged by size:
4594 439 236 174
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2625 3506 4890 5158 1632
Size: 993 881 1384 268 1917
Fragments arranged by size:
1917 1384 993 881 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2620 1184
Size: 1436 4007
AlwNI CAG_nnn'CTG
Cuts at: 2126 3186 4758 2126
Size: 1060 1572 2811
Fragments arranged by size:
2811 1572 1060
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2561 2681 2752 2978 3130 5239 1493
Size: 1068 120 71 226 152 2109 1697
Fragments arranged by size:
2109 1697 1068 226 152 120 71
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2768 1870
Size: 73 114 711 4545
Fragments arranged by size:
4545 711 114 73
BamHI G'GATC_C
Cuts at: 2773 3140 2773
Size: 367 5076
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2764 4219 618
Size: 325 217 627 977 1455 1842
Fragments arranged by size:
1842 1455 977 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2762 1391
Size: 1371 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4054 136
Size: 107 122 71 631 605 2382 1525
Fragments arranged by size:
2382 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2287 3504 4660 1790
Size: 497 1217 1156 2573
Fragments arranged by size:
2573 1217 1156 497
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4103 1239
Size: 2864 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4106 882
Size: 617 2607 2219
Fragments arranged by size:
2607 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3250 3632 4267 275
Size: 53 83 186 936 1717 382 635 1451
Fragments arranged by size:
1717 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2246 3876 4274 4548 4695 5106 2246
Size: 1630 398 274 147 411 2583
Fragments arranged by size:
2583 1630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5443
BseYI C'CCAG_C
Cuts at: 4646 5142 4646
Size: 496 4947
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2166 1163
Size: 573 430 4440
Fragments arranged by size:
4440 573 430
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3504 3589 4660 3504
Size: 85 1071 4287
Fragments arranged by size:
4287 1071 85
BsmI GAATG_Cn'
Cuts at: 2392 2954 3047 2392
Size: 562 93 4788
Fragments arranged by size:
4788 562 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2733 2733
Size: 5443
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 2762 3504 3589 4660 1391
Size: 399 497 475 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 497 475 399 85
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2465 2798 844
Size: 1621 333 3489
Fragments arranged by size:
3489 1621 333
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 1394
Size: 429 1507 3507
Fragments arranged by size:
3507 1507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4112 65
Size: 1183 2690 174 1396
Fragments arranged by size:
2690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 4087 4274 1700
Size: 110 214 2063 187 2869
Fragments arranged by size:
2869 2063 214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2267 2773 3140 3525 3542 4994 5080 5092
Size: 295 506 367 385 17 1452 86 12
Cuts at: 5092 5438 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 506 385 367 346 295 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2451 2451
Size: 5443
BtgI C'CryG_G
Cuts at: 513 1065 2011 513
Size: 552 946 3945
Fragments arranged by size:
3945 946 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 2959 3753 3773 2499
Size: 460 794 20 4169
Fragments arranged by size:
4169 794 460 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2494 2747 2494
Size: 253 5190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2890 3779 5408
Size: 54 1425 50 275 18 1060 889 1629
Cuts at: 5408 8
Size: 43
Fragments arranged by size:
1629 1425 1060 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 3373 1353
Size: 1080 940 3423
Fragments arranged by size:
3423 1080 940
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4030 4404 4550 387
Size: 1064 138 2441 374 146 1280
Fragments arranged by size:
2441 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2625 3506 4103 4890 5158 1239
Size: 393 993 881 597 787 268 1524
Fragments arranged by size:
1524 993 881 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2447 2447
Size: 5443
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 2025 4590 1678
Size: 347 2565 2531
Fragments arranged by size:
2565 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2698 2730 4084
Size: 32 135 32 680 32 414 32 1354
Cuts at: 4084 4116 4158 4190 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1354 680 414 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2529 2787 3033 669
Size: 988 281 591 258 246 3079
Fragments arranged by size:
3079 988 591 281 258 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 2625 3042 2625
Size: 417 5026
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2635 4557 4741 1305
Size: 1330 1922 184 2007
Fragments arranged by size:
2007 1922 1330 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3621
Fragments arranged by size:
3621 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3760 3919 518
Size: 648 2235 359 159 2042
Fragments arranged by size:
2235 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2258 2507 2859 3536 4684 4929 1606
Size: 652 249 352 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 652 352 249 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4891
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2344 2607 2801 4346 1132
Size: 97 1115 263 194 1545 2229
Fragments arranged by size:
2229 1545 1115 263 194 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PstI C_TGCA'G
Cuts at: 830 2476 2506 2795 830
Size: 1646 30 289 3478
Fragments arranged by size:
3478 1646 289 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
PvuII CAG'CTG
Cuts at: 2258 2258
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SapI GCTCTTCn'nnn_
Cuts at: 2433 2433
Size: 5443
SbfI CC_TGCA'GG
Cuts at: 2476 2795 2476
Size: 319 5124
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2472 2502 2791 3926 4607 4798 826
Size: 219 1427 30 289 1135 681 191 1471
Fragments arranged by size:
1471 1427 1135 681 289 219 191 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2770 1872
Size: 898 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3547 4448 4710 820
Size: 197 926 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 2401 1517
Size: 884 4559
StyI C'CwwG_G
Cuts at: 513 1065 2882 513
Size: 552 1817 3074
Fragments arranged by size:
3074 1817 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3470 3487 3640 3825 4270 325
Size: 1255 1890 17 153 185 445 1498
Fragments arranged by size:
1890 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3689 5401 96
Size: 274 80 33 51 1443 1712 1712 138
Fragments arranged by size:
1712 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2128 2160 2330 2362 5403 5435 2128
Size: 32 170 32 3041 32 2136
Fragments arranged by size:
3041 2136 170 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2768 1870
Size: 898 4545
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI