Restriction map for
pFN21AB6980
(Circular) (Six-base) MAPSORT of: pFN21AB6980.seq Check: 4534 from: 1 to: 5440
pFN21AB6980 5440 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2371 2371
Size: 5440
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 2783 1937
Size: 223 623 4594
Fragments arranged by size:
4594 623 223
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2230 3503 4887 5155 1632
Size: 598 1273 1384 268 1917
Fragments arranged by size:
1917 1384 1273 598 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 2287 4169 2287
Size: 1882 3558
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 3183 4755 3183
Size: 1572 3868
ApaI G_GGCC'C
Cuts at: 2215 2215
Size: 5440
ApaLI G'TGCA_C
Cuts at: 2675 3497 4653 2675
Size: 822 1156 3462
Fragments arranged by size:
3462 1156 822
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AscI GG'CGCG_CC
Cuts at: 2056 2056
Size: 5440
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 2193 2667 2765 1870
Size: 73 244 6 474 98 4545
Fragments arranged by size:
4545 474 244 98 73 6
BamHI G'GATC_C
Cuts at: 2770 3137 2770
Size: 367 5073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2761 4216 618
Size: 325 217 627 974 1455 1842
Fragments arranged by size:
1842 1455 974 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2186 2194 2200 2215 2759 1391
Size: 631 164 8 6 15 544 4072
Fragments arranged by size:
4072 631 544 164 15 8 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4469
BbvCI CC'TCA_GC
Cuts at: 2522 2522
Size: 5440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3332 4542 2448
Size: 884 1210 3346
Fragments arranged by size:
3346 1210 884
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2435 4051 136
Size: 107 122 71 631 605 763 1616 1525
Fragments arranged by size:
1616 1525 763 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2485 2857 2485
Size: 372 5068
Bme1580I G_kGCm'C
Cuts at: 1790 2215 2679 3501 4657 1790
Size: 425 464 822 1156 2573
Fragments arranged by size:
2573 1156 822 464 425
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2654 4100 1239
Size: 797 618 1446 2579
Fragments arranged by size:
2579 1446 797 618
Bpu10I CC'TnA_GC
Cuts at: 2522 2522
Size: 5440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2621 3565 4430 4728 2621
Size: 944 865 298 3333
Fragments arranged by size:
3333 944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 2369 5434 2369
Size: 3065 2375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3247 3629 4264 275
Size: 53 83 186 936 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3873 4271 4545 4692 5103 3873
Size: 398 274 147 411 4210
Fragments arranged by size:
4210 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 2342 2372 2627
Size: 30 165 30 606 30 303 30 255
Cuts at: 2627 2657 1178
Size: 30 3961
Fragments arranged by size:
3961 606 303 255 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5440
BseYI C'CCAG_C
Cuts at: 2553 2731 4643 5139 2553
Size: 178 1912 496 2854
Fragments arranged by size:
2854 1912 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 2399 2603 1163
Size: 573 597 66 204 4000
Fragments arranged by size:
4000 597 573 204 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2890 3651 4679 1540
Size: 275 186 889 761 1028 2301
Fragments arranged by size:
2301 1028 889 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2486 2679 3501 3586 4657 2022
Size: 464 193 822 85 1071 2805
Fragments arranged by size:
2805 1071 822 464 193 85
BsmI GAATG_Cn'
Cuts at: 2428 2951 3044 2428
Size: 523 93 4824
Fragments arranged by size:
4824 523 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2186 2194 2200 2215 2486 2679
Size: 399 232 164 8 6 15 271 193
Cuts at: 2679 2759 3501 3586 4657 1391
Size: 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 271 232 193 164
85 80 15 8 6
BspEI T'CCGG_A
Cuts at: 5103 5103
Size: 5440
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2371 2795 844
Size: 1527 424 3489
Fragments arranged by size:
3489 1527 424
BsrBI CCG'CTC
Cuts at: 1394 1823 2638 3327 1394
Size: 429 815 689 3507
Fragments arranged by size:
3507 815 689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3935 4109 65
Size: 1183 2687 174 1396
Fragments arranged by size:
2687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 2056 1925
Size: 131 5309
BssSI C'ACGA_G
Cuts at: 2171 2621 3500 4512 2171
Size: 450 879 1012 3099
Fragments arranged by size:
3099 1012 879 450
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2710 794
Size: 1916 3524
BstBI TT'CG_AA
Cuts at: 2325 4244 2325
Size: 1919 3521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 2149
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2076 2175 2770 3137 3522 3539 4991 5077
Size: 104 99 595 367 385 17 1452 86
Cuts at: 5077 5089 5435 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 595 385 367 346 104 99
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2581 2581
Size: 5440
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 2956 3750 3770 2445
Size: 511 794 20 4115
Fragments arranged by size:
4115 794 511 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2270 2887 3776
Size: 54 1425 50 275 18 440 617 889
Cuts at: 3776 5405 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 617 440 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2887 1537
Size: 275 1075 4090
Fragments arranged by size:
4090 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3370 1353
Size: 2017 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2303 2531 4027 4401 4547 387
Size: 1064 138 714 228 1496 374 146 1280
Fragments arranged by size:
1496 1280 1064 714 374 228 146 138
EcoICRI GAG'CTC
Cuts at: 2020 2020
Size: 5440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2230 2654 3503 4100 4887 5155
Size: 393 404 194 424 849 597 787 268
Cuts at: 5155 1239
Size: 1524
Fragments arranged by size:
1524 849 787 597 424 404 393 268
194
EcoO109I rG'GnC_Cy
Cuts at: 2211 2212 2403 2211
Size: 1 191 5248
Fragments arranged by size:
5248 191 1
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
EcoRV GAT'ATC
Cuts at: 2371 2371
Size: 5440
FspI TGC'GCA
Cuts at: 2072 3946 2072
Size: 1874 3566
HaeII r_GCGC'y
Cuts at: 1678 4587 1678
Size: 2909 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2273 2305 4081
Size: 32 135 32 437 32 232 32 1776
Cuts at: 4081 4113 4155 4187 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1776 437 232 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 2625 4554 4738 1305
Size: 702 618 1929 184 2007
Fragments arranged by size:
2007 1929 702 618 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3618
Fragments arranged by size:
3618 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2712 3398 3757 3916 518
Size: 648 1546 686 359 159 2042
Fragments arranged by size:
2042 1546 686 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2347 2489 2543 2557 2856 3533 4681 4926
Size: 741 142 54 14 299 677 1148 245
Cuts at: 4926 1606
Size: 2120
Fragments arranged by size:
2120 1148 741 677 299 245 142 54
14
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2711 2798 4343 1132
Size: 97 1482 87 1545 2229
Fragments arranged by size:
2229 1545 1482 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5440
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2403 2403
Size: 5440
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PspOMI G'GGCC_C
Cuts at: 2211 2211
Size: 5440
PspXI vC'TCGA_Gb
Cuts at: 2193 2193
Size: 5440
PstI C_TGCA'G
Cuts at: 830 2310 2792 830
Size: 1480 482 3478
Fragments arranged by size:
3478 1480 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
PvuII CAG'CTG
Cuts at: 2347 2489 2543 2557 2347
Size: 142 54 14 5230
Fragments arranged by size:
5230 142 54 14
SacI G_AGCT'C
Cuts at: 2022 2022
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2306 2387 2788 3923 4604 4795 826
Size: 219 1261 81 401 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 681 401 219 191 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2189 2767 1872
Size: 317 578 4545
Fragments arranged by size:
4545 578 317
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2193 2600 3544 4445 4707 820
Size: 197 926 250 407 944 901 262 1553
Fragments arranged by size:
1553 944 926 901 407 262 250 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 2720 1517
Size: 1203 4237
StyI C'CwwG_G
Cuts at: 513 1065 2004 2142 2879 513
Size: 552 939 138 737 3074
Fragments arranged by size:
3074 939 737 552 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2367 3467 3484 3637 3822 4267 325
Size: 1255 787 1100 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1100 787 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 5400 5432 2342
Size: 32 3026 32 2350
Fragments arranged by size:
3026 2350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 2193 1943
Size: 250 5190
XmaI C'CCGG_G
Cuts at: 1870 2187 2765 1870
Size: 317 578 4545
Fragments arranged by size:
4545 578 317
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI