Restriction map for
pFN21AB6979
(Circular) (Six-base) MAPSORT of: pFN21AB6979.seq Check: 7055 from: 1 to: 5359
pFN21AB6979 5359 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3169 278
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 1937
Size: 765 4594
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2599 3422 4806 5074 1632
Size: 437 530 823 1384 268 1917
Fragments arranged by size:
1917 1384 823 530 437 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 2298 2653 4258 5235 1203
Size: 1095 355 1605 977 1327
Fragments arranged by size:
1605 1327 1095 977 355
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 4088 4088
Size: 5359
AleI CACnn'nnGTG
Cuts at: 1166 2387 2596 1166
Size: 1221 209 3929
Fragments arranged by size:
3929 1221 209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2381 2405 1184
Size: 1197 24 4138
Fragments arranged by size:
4138 1197 24
AlwNI CAG_nnn'CTG
Cuts at: 3102 4674 3102
Size: 1572 3787
ApaI G_GGCC'C
Cuts at: 2043 2077 2043
Size: 34 5325
ApaLI G'TGCA_C
Cuts at: 2380 3416 4572 2380
Size: 1036 1156 3167
Fragments arranged by size:
3167 1156 1036
ApoI r'AATT_y
Cuts at: 1493 2668 2894 3046 5155 1493
Size: 1175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3913 160
Size: 872 2881 1606
Fragments arranged by size:
2881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 2268 2684 1870
Size: 73 189 136 416 4545
Fragments arranged by size:
4545 416 189 136 73
BamHI G'GATC_C
Cuts at: 2457 2689 3056 2457
Size: 232 367 4760
Fragments arranged by size:
4760 367 232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 2152 2389 2598 2680
Size: 325 217 627 341 24 237 209 82
Cuts at: 2680 4135 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 341 325 237 217 209
82 24
BanII G_rGCy'C
Cuts at: 1391 2043 2077 2678 1391
Size: 652 34 601 4072
Fragments arranged by size:
4072 652 601 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2072 928
Size: 971 173 4215
Fragments arranged by size:
4215 971 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 1903
Size: 34 1613 34 3678
Fragments arranged by size:
3678 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4461 3251
Size: 1210 4149
BclI T'GATC_A
Cuts at: 1548 2400 1548
Size: 852 4507
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3970 136
Size: 107 122 71 631 605 2298 1525
Fragments arranged by size:
2298 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5354 5354
Size: 5359
BlpI GC'TnA_GC
Cuts at: 2180 2776 2180
Size: 596 4763
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2077 2384 2603 3420 4576 1790
Size: 253 34 307 219 817 1156 2573
Fragments arranged by size:
2573 1156 817 307 253 219 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2332 3090 4048
Size: 416 53 83 399 24 915 758 958
Cuts at: 4048 442
Size: 1753
Fragments arranged by size:
1753 958 915 758 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2602 4019 1239
Size: 1363 1417 2579
Fragments arranged by size:
2579 1417 1363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2177 2354 2474 3484 4349 4647 2177
Size: 177 120 1010 865 298 2889
Fragments arranged by size:
2889 1010 865 298 177 120
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4022 882
Size: 617 2523 2219
Fragments arranged by size:
2523 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2654 3150 493
Size: 711 1450 496 2702
Fragments arranged by size:
2702 1450 711 496
BsaBI GATnn'nnATC
Cuts at: 5353 5353
Size: 5359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3166 3548 4183 275
Size: 53 83 186 936 1633 382 635 1451
Fragments arranged by size:
1633 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3792 4190 4464 4611 5022 3792
Size: 398 274 147 411 4129
Fragments arranged by size:
4129 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 2432 2462 1178
Size: 30 165 30 843 30 156 30 4075
Fragments arranged by size:
4075 843 165 156 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 2487 2550 1186
Size: 1291 10 63 3995
Fragments arranged by size:
3995 1291 63 10
BseYI C'CCAG_C
Cuts at: 4562 5058 4562
Size: 496 4863
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4786
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2809 3570 4598 1540
Size: 275 186 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2384 3420 3505 4576 2370
Size: 14 1036 85 1071 3153
Fragments arranged by size:
3153 1071 1036 85 14
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2077 2370 2384 2603 2678 3420
Size: 399 253 34 293 14 219 75 742
Cuts at: 3420 3505 4576 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 293 253 219 85
75 34 14
BspEI T'CCGG_A
Cuts at: 5022 5022
Size: 5359
BspHI T'CATG_A
Cuts at: 2061 3248 4978 2061
Size: 1187 1730 2442
Fragments arranged by size:
2442 1730 1187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2577 2714 844
Size: 1733 137 3489
Fragments arranged by size:
3489 1733 137
BsrBI CCG'CTC
Cuts at: 1394 1823 2443 3246 1394
Size: 429 620 803 3507
Fragments arranged by size:
3507 803 620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 4028 65
Size: 1183 2606 174 1396
Fragments arranged by size:
2606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4003 4190 1700
Size: 110 2193 187 2869
Fragments arranged by size:
2869 2193 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5359
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5359
BssSI C'ACGA_G
Cuts at: 3419 4431 3419
Size: 1012 4347
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5359
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5359
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 2563 2362
Size: 201 5158
BstYI r'GATC_y
Cuts at: 1972 2457 2689 3056 3441 3458 4910 4996 5008
Size: 485 232 367 385 17 1452 86 12
Cuts at: 5008 5354 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 485 385 367 346 232 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2157 2157
Size: 5359
BtgI C'CryG_G
Cuts at: 513 1065 2004 2250 513
Size: 552 939 246 3622
Fragments arranged by size:
3622 939 552 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4620
Fragments arranged by size:
4620 683 56
BtsI GCAGTG_nn'
Cuts at: 2875 3669 3689 2875
Size: 794 20 4545
Fragments arranged by size:
4545 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5324
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2806 3695 5324
Size: 54 1425 50 275 18 976 889 1629
Cuts at: 5324 8
Size: 43
Fragments arranged by size:
1629 1425 976 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2806 1537
Size: 275 994 4090
Fragments arranged by size:
4090 994 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3289 1353
Size: 1936 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3946 4320 4466 387
Size: 1064 138 2357 374 146 1280
Fragments arranged by size:
2357 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2599 2602 3422 4019 4806 5074
Size: 393 437 530 3 820 597 787 268
Cuts at: 5074 1239
Size: 1524
Fragments arranged by size:
1524 820 787 597 530 437 393 268
3
EcoO109I rG'GnC_Cy
Cuts at: 2039 2073 2074 2374 2567 2039
Size: 34 1 300 193 4831
Fragments arranged by size:
4831 300 193 34 1
EcoRI G'AATT_C
Cuts at: 2668 3046 2668
Size: 378 4981
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 4506 1678
Size: 2828 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2173 2205 2246 2278 4000
Size: 32 135 32 601 32 41 32 1722
Cuts at: 4000 4032 4074 4106 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1722 601 135 42 41 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2949 669
Size: 988 281 765 246 3079
Fragments arranged by size:
3079 988 765 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5359
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2958 2958
Size: 5359
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4473 4657 1305
Size: 3168 184 2007
Fragments arranged by size:
3168 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3537
Fragments arranged by size:
3537 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 2596 3317 3676 3835 518
Size: 648 1221 209 721 359 159 2042
Fragments arranged by size:
2042 1221 721 648 359 209 159
MspA1I CmG'CkG
Cuts at: 1606 2294 2775 3452 4600 4845 1606
Size: 688 481 677 1148 245 2120
Fragments arranged by size:
2120 1148 688 677 481 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3868
Fragments arranged by size:
3868 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2302 2717 4262 1132
Size: 97 1073 415 1545 2229
Fragments arranged by size:
2229 1545 1073 415 97
PasI CC'CwG_GG
Cuts at: 1348 2571 1348
Size: 1223 4136
PciI A'CATG_T
Cuts at: 2298 4258 2298
Size: 1960 3399
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5359
PfoI T'CCnGG_A
Cuts at: 2097 2436 2097
Size: 339 5020
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 4941 4973 3428
Size: 32 1481 32 3814
Fragments arranged by size:
3814 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2567 2567
Size: 5359
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5359
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PspOMI G'GGCC_C
Cuts at: 2039 2073 2039
Size: 34 5325
PstI C_TGCA'G
Cuts at: 830 2235 2711 830
Size: 1405 476 3478
Fragments arranged by size:
3478 1405 476
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
PvuII CAG'CTG
Cuts at: 2294 2294
Size: 5359
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SanDI GG'GwC_CC
Cuts at: 2567 2567
Size: 5359
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 3607 5319 3607
Size: 1712 3647
SfcI C'TryA_G
Cuts at: 826 1045 2231 2707 3842 4523 4714 826
Size: 219 1186 476 1135 681 191 1471
Fragments arranged by size:
1471 1186 1135 681 476 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5359
SmaI CCC'GGG
Cuts at: 1872 2686 1872
Size: 814 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 2369 2489 3463 4364 4626
Size: 197 926 249 177 120 974 901 262
Cuts at: 4626 820
Size: 1553
Fragments arranged by size:
1553 974 926 901 262 249 197 177
120
SnaBI TAC'GTA
Cuts at: 493 3150 493
Size: 2657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2717 1132
Size: 1585 3774
SspI AAT'ATT
Cuts at: 5 52 3283 5
Size: 47 3231 2081
Fragments arranged by size:
3231 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5359
StyI C'CwwG_G
Cuts at: 513 1065 2004 2385 2798 513
Size: 552 939 381 413 3074
Fragments arranged by size:
3074 939 552 413 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2467 3386 3403 3556 3741 4186 325
Size: 1255 887 919 17 153 185 445 1498
Fragments arranged by size:
1498 1255 919 887 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3605 5317 96
Size: 274 80 33 51 1443 1628 1712 138
Fragments arranged by size:
1712 1628 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5319 5351 5319
Size: 32 5327
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5359
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 2468
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2684 1870
Size: 814 4545
XmnI GAAnn'nnTTC
Cuts at: 3488 3488
Size: 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3167 276
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI