Restriction map for
pFN21AB6975
(Circular) (Six-base) MAPSORT of: pFN21AB6975.seq Check: 9701 from: 1 to: 5356
pFN21AB6975 5356 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3166 278
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2699 1937
Size: 762 4594
Acc65I G'GTAC_C
Cuts at: 2677 2677
Size: 5356
AclI AA'CG_TT
Cuts at: 1563 3483 3856 1563
Size: 1920 373 3063
Fragments arranged by size:
3063 1920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3419 4803 5071 1632
Size: 977 810 1384 268 1917
Fragments arranged by size:
1917 1384 977 810 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5159
AflIII A'CryG_T
Cuts at: 1203 4255 5232 1203
Size: 3052 977 1327
Fragments arranged by size:
3052 1327 977
AgeI A'CCGG_T
Cuts at: 4187 4187
Size: 5356
AhdI GACnn_n'nnGTC
Cuts at: 4085 4085
Size: 5356
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5356
AlwNI CAG_nnn'CTG
Cuts at: 2508 3099 4671 2508
Size: 591 1572 3193
Fragments arranged by size:
3193 1572 591
ApaLI G'TGCA_C
Cuts at: 3413 4569 3413
Size: 1156 4200
ApoI r'AATT_y
Cuts at: 1493 2243 2379 2544 2665 2891 3043 5152 1493
Size: 750 136 165 121 226 152 2109 1697
Fragments arranged by size:
2109 1697 750 226 165 152 136 121
AseI AT'TA_AT
Cuts at: 160 1032 3910 160
Size: 872 2878 1606
Fragments arranged by size:
2878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2681 1870
Size: 73 92 646 4545
Fragments arranged by size:
4545 646 92 73
BamHI G'GATC_C
Cuts at: 2686 3053 2686
Size: 367 4989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2677 4132 618
Size: 325 217 627 890 1455 1842
Fragments arranged by size:
1842 1455 890 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2352 2675 1391
Size: 961 323 4072
Fragments arranged by size:
4072 961 323
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3547 3581 1903
Size: 34 1610 34 3678
Fragments arranged by size:
3678 1610 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3248 4458 3248
Size: 1210 4146
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3967 136
Size: 107 122 71 631 605 2295 1525
Fragments arranged by size:
2295 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5351 5351
Size: 5356
BlpI GC'TnA_GC
Cuts at: 2773 2773
Size: 5356
Bme1580I G_kGCm'C
Cuts at: 1790 3417 4573 1790
Size: 1627 1156 2573
Fragments arranged by size:
2573 1627 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3087 4045 442
Size: 416 53 83 399 24 1670 958 1753
Fragments arranged by size:
1753 1670 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4016 1239
Size: 2777 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2470 3481 4346 4644 2470
Size: 1011 865 298 3182
Fragments arranged by size:
3182 1011 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2480 4019 882
Size: 617 981 1539 2219
Fragments arranged by size:
2219 1539 981 617
BsaAI yAC'GTr
Cuts at: 493 1204 3147 493
Size: 711 1943 2702
Fragments arranged by size:
2702 1943 711
BsaBI GATnn'nnATC
Cuts at: 5350 5350
Size: 5356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3163 3545 4180 275
Size: 53 83 186 936 1630 382 635 1451
Fragments arranged by size:
1630 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3789 4187 4461 4608 5019 3789
Size: 398 274 147 411 4126
Fragments arranged by size:
4126 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5131
Fragments arranged by size:
5131 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5356
BseYI C'CCAG_C
Cuts at: 2080 4559 5055 2080
Size: 2479 496 2381
Fragments arranged by size:
2479 2381 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4783
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2806 3567 4595 1540
Size: 275 186 805 761 1028 2301
Fragments arranged by size:
2301 1028 805 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2352 3417 3502 4573 2352
Size: 1065 85 1071 3135
Fragments arranged by size:
3135 1071 1065 85
BsmI GAATG_Cn'
Cuts at: 2867 2960 2867
Size: 93 5263
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2675 3417 3502 4573 1391
Size: 399 562 323 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 399 323 85
BspEI T'CCGG_A
Cuts at: 5019 5019
Size: 5356
BspHI T'CATG_A
Cuts at: 2149 3245 4975 2149
Size: 1096 1730 2530
Fragments arranged by size:
2530 1730 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2711 844
Size: 1867 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3243 1394
Size: 429 1420 3507
Fragments arranged by size:
3507 1420 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2115 3851 4025 65
Size: 1183 867 1736 174 1396
Fragments arranged by size:
1736 1396 1183 867 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4000 4187 1700
Size: 110 2190 187 2869
Fragments arranged by size:
2869 2190 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5356
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5356
BssSI C'ACGA_G
Cuts at: 3416 4428 3416
Size: 1012 4344
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2269 794
Size: 1475 3881
BstBI TT'CG_AA
Cuts at: 4160 4160
Size: 5356
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5356
BstYI r'GATC_y
Cuts at: 1972 2686 3053 3438 3455 4907 4993 5005 5351
Size: 714 367 385 17 1452 86 12 346
Cuts at: 5351 1972
Size: 1977
Fragments arranged by size:
1977 1452 714 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3865
Fragments arranged by size:
3865 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4617
Fragments arranged by size:
4617 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 2872 3666 3686 2226
Size: 646 794 20 3896
Fragments arranged by size:
3896 794 646 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5321
DraI TTT'AAA
Cuts at: 2344 2660 2344
Size: 316 5040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4363 809
Size: 507 3047 1802
Fragments arranged by size:
3047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 2803 3692
Size: 54 1425 50 275 18 403 570 889
Cuts at: 3692 5321 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 570 403 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2803 1537
Size: 275 991 4090
Fragments arranged by size:
4090 991 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2585 2633 3286 1353
Size: 1232 48 653 3423
Fragments arranged by size:
3423 1232 653 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 2414 3943 4317 4463 387
Size: 1064 138 765 60 1529 374 146 1280
Fragments arranged by size:
1529 1280 1064 765 374 146 138 60
EcoICRI GAG'CTC
Cuts at: 2350 2350
Size: 5356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 3419 4016 4803 5071 1239
Size: 393 977 810 597 787 268 1524
Fragments arranged by size:
1524 977 810 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2243 2665 3043 2243
Size: 422 378 4556
Fragments arranged by size:
4556 422 378
FspI TGC'GCA
Cuts at: 3862 3862
Size: 5356
HaeII r_GCGC'y
Cuts at: 1678 4503 1678
Size: 2825 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 3997 4029 4071
Size: 32 135 32 731 32 1662 32 42
Cuts at: 4071 4103 1373
Size: 32 2626
Fragments arranged by size:
2626 1662 731 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2700 2946 669
Size: 988 281 762 246 3079
Fragments arranged by size:
3079 988 762 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5356
HpaI GTT'AAC
Cuts at: 2946 2946
Size: 5356
KpnI G_GTAC'C
Cuts at: 2681 2681
Size: 5356
MfeI C'AATT_G
Cuts at: 2955 2955
Size: 5356
MluI A'CGCG_T
Cuts at: 5232 5232
Size: 5356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4470 4654 1305
Size: 3165 184 2007
Fragments arranged by size:
3165 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2235 10
Size: 54 1425 343 403 3131
Fragments arranged by size:
3131 1425 403 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2373 3314 3673 3832 518
Size: 648 1086 121 941 359 159 2042
Fragments arranged by size:
2042 1086 941 648 359 159 121
MspA1I CmG'CkG
Cuts at: 1606 2028 2772 3449 4597 4842 1606
Size: 422 744 677 1148 245 2120
Fragments arranged by size:
2120 1148 744 677 422 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3865
Fragments arranged by size:
3865 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5356
NotI GC'GGCC_GC
Cuts at: 2803 2803
Size: 5356
NspI r_CATG'y
Cuts at: 1132 1229 2272 2714 4259 1132
Size: 97 1043 442 1545 2229
Fragments arranged by size:
2229 1545 1043 442 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5356
PciI A'CATG_T
Cuts at: 4255 4255
Size: 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5356
PmeI GTTT'AAAC
Cuts at: 2660 2660
Size: 5356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 4938 4970 3425
Size: 32 1481 32 3811
Fragments arranged by size:
3811 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2183 1613
Size: 570 4786
PsiI TTA'TAA
Cuts at: 2175 2926 2175
Size: 751 4605
PstI C_TGCA'G
Cuts at: 830 2497 2708 830
Size: 1667 211 3478
Fragments arranged by size:
3478 1667 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5356
PvuII CAG'CTG
Cuts at: 2028 2028
Size: 5356
SacI G_AGCT'C
Cuts at: 2352 2352
Size: 5356
SalI G'TCGA_C
Cuts at: 1936 2698 1936
Size: 762 4594
SbfI CC_TGCA'GG
Cuts at: 2708 2708
Size: 5356
ScaI AGT'ACT
Cuts at: 3604 5316 3604
Size: 1712 3644
SfcI C'TryA_G
Cuts at: 826 1045 2160 2436 2493 2704 3839 4520 4711
Size: 219 1115 276 57 211 1135 681 191
Cuts at: 4711 826
Size: 1471
Fragments arranged by size:
1471 1135 1115 681 276 219 211 191
57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5356
SmaI CCC'GGG
Cuts at: 1872 2037 2683 1872
Size: 165 646 4545
Fragments arranged by size:
4545 646 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2449 3460 4361 4623 820
Size: 197 926 506 1011 901 262 1553
Fragments arranged by size:
1553 1011 926 901 506 262 197
SnaBI TAC'GTA
Cuts at: 493 3147 493
Size: 2654 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5356
SphI G_CATG'C
Cuts at: 1132 2714 1132
Size: 1582 3774
SspI AAT'ATT
Cuts at: 5 52 3280 5
Size: 47 3228 2081
Fragments arranged by size:
3228 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5356
StyI C'CwwG_G
Cuts at: 513 1065 2004 2795 513
Size: 552 939 791 3074
Fragments arranged by size:
3074 939 791 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3383 3400 3553 3738 4183 325
Size: 1255 1803 17 153 185 445 1498
Fragments arranged by size:
1803 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2064 2265 3602
Size: 274 80 33 51 1443 87 201 1337
Cuts at: 3602 5314 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1337 274 201 138 87 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5316 5348 5316
Size: 32 5324
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5356
XbaI T'CTAG_A
Cuts at: 2692 2692
Size: 5356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5356
XmaI C'CCGG_G
Cuts at: 1870 2035 2681 1870
Size: 165 646 4545
Fragments arranged by size:
4545 646 165
XmnI GAAnn'nnTTC
Cuts at: 3485 3485
Size: 5356
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3164 276
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI