Restriction map for
pFN21AB6970
(Circular) (Six-base) MAPSORT of: pFN21AB6970.seq Check: 6060 from: 1 to: 5428
pFN21AB6970 5428 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 3491 4875 5143 1632
Size: 876 983 1384 268 1917
Fragments arranged by size:
1917 1384 983 876 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 2685 4327 5304 1203
Size: 1482 1642 977 1327
Fragments arranged by size:
1642 1482 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 3171 4743 3171
Size: 1572 3856
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2677 2737 2963 3115 5224 1493
Size: 1184 60 226 152 2109 1697
Fragments arranged by size:
2109 1697 1184 226 152 60
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2505 2753 1870
Size: 73 562 248 4545
Fragments arranged by size:
4545 562 248 73
BamHI G'GATC_C
Cuts at: 2121 2758 3125 2121
Size: 637 367 4424
Fragments arranged by size:
4424 637 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 2531 2749 4204 618
Size: 325 217 627 609 135 218 1455 1842
Fragments arranged by size:
1842 1455 627 609 325 218 217 135
BanII G_rGCy'C
Cuts at: 1391 2187 2543 2747 1391
Size: 796 356 204 4072
Fragments arranged by size:
4072 796 356 204
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2586 928
Size: 971 687 3770
Fragments arranged by size:
3770 971 687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 4039 136
Size: 107 122 71 631 605 418 1949 1525
Fragments arranged by size:
1949 1525 631 605 418 122 107 71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 2536 3489 4645 1790
Size: 746 953 1156 2573
Fragments arranged by size:
2573 1156 953 746
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3159 4117 442
Size: 416 53 83 399 24 1742 958 1753
Fragments arranged by size:
1753 1742 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2657 1056
Size: 1601 3827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4088 1239
Size: 2849 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4091 882
Size: 617 2592 2219
Fragments arranged by size:
2592 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 2388 5422 2388
Size: 3034 2394
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3235 3617 4252 275
Size: 53 83 186 936 1702 382 635 1451
Fragments arranged by size:
1702 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3861 4259 4533 4680 5091 3861
Size: 398 274 147 411 4198
Fragments arranged by size:
4198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2035 2065 1178
Size: 30 165 30 632 30 4541
Fragments arranged by size:
4541 632 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2451 2639 1186
Size: 1265 188 3975
Fragments arranged by size:
3975 1265 188
BseYI C'CCAG_C
Cuts at: 2186 2457 4631 5127 2186
Size: 271 2174 496 2487
Fragments arranged by size:
2487 2174 496 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 1163
Size: 573 948 3907
Fragments arranged by size:
3907 948 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2878 3639 4667 1540
Size: 275 186 877 761 1028 2301
Fragments arranged by size:
2301 1028 877 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2543 3489 3574 4645 2543
Size: 946 85 1071 3326
Fragments arranged by size:
3326 1071 946 85
BsmI GAATG_Cn'
Cuts at: 2641 2939 3032 2641
Size: 298 93 5037
Fragments arranged by size:
5037 298 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2536 2543 2747 3489 3574 4645
Size: 399 397 349 7 204 742 85 1071
Cuts at: 4645 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 397 349 204 85
7
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2783 844
Size: 1939 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 1394
Size: 429 1492 3507
Fragments arranged by size:
3507 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4072 4259 1700
Size: 110 2262 187 2869
Fragments arranged by size:
2869 2262 187 110
BsrGI T'GTAC_A
Cuts at: 96 2491 96
Size: 2395 3033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 3488 4500 3488
Size: 1012 4416
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2121 2389 2758 3125 3510 3527 4979 5065
Size: 149 268 369 367 385 17 1452 86
Cuts at: 5065 5077 5423 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 369 367 346 268 149
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4876
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3738 3758 2944
Size: 794 20 4614
Fragments arranged by size:
4614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2310 2345 529
Size: 35 1746 35 3612
Fragments arranged by size:
3612 1746 35 35
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4435 809
Size: 507 3119 1802
Fragments arranged by size:
3119 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 2875 3764
Size: 54 1425 50 275 18 841 204 889
Cuts at: 3764 5393 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 841 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2703 3358 1353
Size: 800 550 655 3423
Fragments arranged by size:
3423 800 655 550
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4015 4389 4535 387
Size: 1064 138 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2541 2541
Size: 5428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 3491 4088 4875 5143 1239
Size: 393 876 983 597 787 268 1524
Fragments arranged by size:
1524 983 876 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2453 2484 2453
Size: 31 5397
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 4575 1678
Size: 2897 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2203 2235 4069 4101 4143
Size: 32 135 32 631 32 1834 32 42
Cuts at: 4143 4175 1373
Size: 32 2626
Fragments arranged by size:
2626 1834 631 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3018 669
Size: 988 281 834 246 3079
Fragments arranged by size:
3079 988 834 281 246
HindIII A'AGCT_T
Cuts at: 748 2099 748
Size: 1351 4077
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 2511 3027 2511
Size: 516 4912
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4542 4726 1305
Size: 3237 184 2007
Fragments arranged by size:
3237 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2673 10
Size: 54 1425 343 841 2765
Fragments arranged by size:
2765 1425 841 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3386 3745 3904 518
Size: 648 2220 359 159 2042
Fragments arranged by size:
2220 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2461 2844 3521 4669 4914 1606
Size: 855 383 677 1148 245 2120
Fragments arranged by size:
2120 1148 855 677 383 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4876
NdeI CA'TA_TG
Cuts at: 387 2469 387
Size: 2082 3346
NheI G'CTAG_C
Cuts at: 1052 2653 1052
Size: 1601 3827
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2689 2786 4331 1132
Size: 97 1460 97 1545 2229
Fragments arranged by size:
2229 1545 1460 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 2685 4327 2685
Size: 1642 3786
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2453 2484 2453
Size: 31 5397
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PspXI vC'TCGA_Gb
Cuts at: 2505 2505
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2780 830
Size: 1950 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 5428
SacI G_AGCT'C
Cuts at: 2543 2543
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SfcI C'TryA_G
Cuts at: 826 1045 2109 2776 3911 4592 4783 826
Size: 219 1064 667 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 681 667 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2505 3532 4433 4695 820
Size: 197 926 562 1027 901 262 1553
Fragments arranged by size:
1553 1027 926 901 562 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2867 513
Size: 552 1802 3074
Fragments arranged by size:
3074 1802 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3455 3472 3625 3810 4255 325
Size: 1255 1875 17 153 185 445 1498
Fragments arranged by size:
1875 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2491 3674 5386
Size: 274 80 33 51 1443 514 1183 1712
Cuts at: 5386 96
Size: 138
Fragments arranged by size:
1712 1443 1183 514 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2589 2621 5388 5420 2589
Size: 32 2767 32 2597
Fragments arranged by size:
2767 2597 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 2505 1943
Size: 562 4866
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 2167 3557 2167
Size: 1390 4038
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI