Restriction map for
pFN21AB6969
(Circular) (Six-base) MAPSORT of: pFN21AB6969.seq Check: 32 from: 1 to: 5353
pFN21AB6969 5353 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3163 278
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2696 1937
Size: 759 4594
Acc65I G'GTAC_C
Cuts at: 2674 2674
Size: 5353
AclI AA'CG_TT
Cuts at: 1563 2652 3480 3853 1563
Size: 1089 828 373 3063
Fragments arranged by size:
3063 1089 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3416 4800 5068 1632
Size: 1784 1384 268 1917
Fragments arranged by size:
1917 1784 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5156
AflIII A'CryG_T
Cuts at: 1203 4252 5229 1203
Size: 3049 977 1327
Fragments arranged by size:
3049 1327 977
AgeI A'CCGG_T
Cuts at: 4184 4184
Size: 5353
AhdI GACnn_n'nnGTC
Cuts at: 4082 4082
Size: 5353
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5353
AlwNI CAG_nnn'CTG
Cuts at: 2048 3096 4668 2048
Size: 1048 1572 2733
Fragments arranged by size:
2733 1572 1048
ApaI G_GGCC'C
Cuts at: 2287 2287
Size: 5353
ApaLI G'TGCA_C
Cuts at: 3410 4566 3410
Size: 1156 4197
ApoI r'AATT_y
Cuts at: 1493 2438 2662 2888 3040 5149 1493
Size: 945 224 226 152 2109 1697
Fragments arranged by size:
2109 1697 945 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 3907 160
Size: 872 2875 1606
Fragments arranged by size:
2875 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 1870
Size: 73 735 4545
Fragments arranged by size:
4545 735 73
BamHI G'GATC_C
Cuts at: 2683 3050 2683
Size: 367 4986
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2674 4129 618
Size: 325 217 627 887 1455 1842
Fragments arranged by size:
1842 1455 887 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2287 2565 2672 1391
Size: 896 278 107 4072
Fragments arranged by size:
4072 896 278 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3544 3578 1903
Size: 34 1607 34 3678
Fragments arranged by size:
3678 1607 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3245 4455 3245
Size: 1210 4143
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5353
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3964 136
Size: 107 122 71 631 605 2292 1525
Fragments arranged by size:
2292 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2058 2223 5348 2058
Size: 165 3125 2063
Fragments arranged by size:
3125 2063 165
BlpI GC'TnA_GC
Cuts at: 2770 2770
Size: 5353
Bme1580I G_kGCm'C
Cuts at: 1790 2287 3414 4570 1790
Size: 497 1127 1156 2573
Fragments arranged by size:
2573 1156 1127 497
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2356 3084 4042
Size: 416 53 83 399 24 939 728 958
Cuts at: 4042 442
Size: 1753
Fragments arranged by size:
1753 958 939 728 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2568 2594 4013 1239
Size: 1329 26 1419 2579
Fragments arranged by size:
2579 1419 1329 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3478 4343 4641 3478
Size: 865 298 4190
Fragments arranged by size:
4190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4016 882
Size: 617 2517 2219
Fragments arranged by size:
2517 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3144 493
Size: 711 1940 2702
Fragments arranged by size:
2702 1940 711
BsaBI GATnn'nnATC
Cuts at: 5347 5347
Size: 5353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3160 3542 4177 275
Size: 53 83 186 936 1627 382 635 1451
Fragments arranged by size:
1627 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3786 4184 4458 4605 5016 3786
Size: 398 274 147 411 4123
Fragments arranged by size:
4123 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2566 2596 1178
Size: 30 165 30 1163 30 3935
Fragments arranged by size:
3935 1163 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5353
BseYI C'CCAG_C
Cuts at: 2048 2083 2623 4556 5052 2048
Size: 35 540 1933 496 2349
Fragments arranged by size:
2349 1933 540 496 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4780
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3564 4592 1540
Size: 275 186 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2162 2584 3414 3499 4570 2162
Size: 422 830 85 1071 2945
Fragments arranged by size:
2945 1071 830 422 85
BsmI GAATG_Cn'
Cuts at: 2575 2864 2957 2575
Size: 289 93 4971
Fragments arranged by size:
4971 289 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2287 2565 2584 2672 3414 3499
Size: 399 372 125 278 19 88 742 85
Cuts at: 3499 4570 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 278 125 88
85 19
BspEI T'CCGG_A
Cuts at: 5016 5016
Size: 5353
BspHI T'CATG_A
Cuts at: 3242 4972 3242
Size: 1730 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2708 844
Size: 1864 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2171 2248 3240 1394
Size: 429 348 77 992 3507
Fragments arranged by size:
3507 992 429 348 77
BsrDI GCAATG_nn'
Cuts at: 65 1248 2388 2514 3848 4022 65
Size: 1183 1140 126 1334 174 1396
Fragments arranged by size:
1396 1334 1183 1140 174 126
BsrFI r'CCGG_y
Cuts at: 1700 1810 3997 4184 1700
Size: 110 2187 187 2869
Fragments arranged by size:
2869 2187 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5353
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5353
BssSI C'ACGA_G
Cuts at: 2144 3413 4425 2144
Size: 1269 1012 3072
Fragments arranged by size:
3072 1269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2504 2579 794
Size: 1710 75 3568
Fragments arranged by size:
3568 1710 75
BstBI TT'CG_AA
Cuts at: 4157 4157
Size: 5353
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5353
BstYI r'GATC_y
Cuts at: 1972 2058 2223 2683 3050 3435 3452 4904 4990
Size: 86 165 460 367 385 17 1452 86
Cuts at: 4990 5002 5348 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 460 385 367 346 165 86
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2433 513
Size: 552 939 429 3433
Fragments arranged by size:
3433 939 552 429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4614
Fragments arranged by size:
4614 683 56
BtsI GCAGTG_nn'
Cuts at: 2869 3663 3683 2869
Size: 794 20 4539
Fragments arranged by size:
4539 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5353
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5318
DraI TTT'AAA
Cuts at: 2657 2657
Size: 5353
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2210 4360 809
Size: 507 894 2150 1802
Fragments arranged by size:
2150 1802 894 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 2800 3689
Size: 54 1425 50 275 18 588 382 889
Cuts at: 3689 5318 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 588 382 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2800 1537
Size: 275 988 4090
Fragments arranged by size:
4090 988 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3283 1353
Size: 1930 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3940 4314 4460 387
Size: 1064 138 2351 374 146 1280
Fragments arranged by size:
2351 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2568 2594 3416 4013 4800 5068 1239
Size: 393 936 26 822 597 787 268 1524
Fragments arranged by size:
1524 936 822 787 597 393 268 26
EcoO109I rG'GnC_Cy
Cuts at: 2283 2283
Size: 5353
EcoRI G'AATT_C
Cuts at: 2438 2662 3040 2438
Size: 224 378 4751
Fragments arranged by size:
4751 378 224
FspI TGC'GCA
Cuts at: 3859 3859
Size: 5353
HaeII r_GCGC'y
Cuts at: 1678 4500 1678
Size: 2822 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3994 4026 4068 4100 1373
Size: 32 135 32 2422 32 42 32 2626
Fragments arranged by size:
2626 2422 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2697 2943 669
Size: 988 281 759 246 3079
Fragments arranged by size:
3079 988 759 281 246
HindIII A'AGCT_T
Cuts at: 748 2546 748
Size: 1798 3555
HpaI GTT'AAC
Cuts at: 2943 2943
Size: 5353
KpnI G_GTAC'C
Cuts at: 2678 2678
Size: 5353
MfeI C'AATT_G
Cuts at: 2163 2952 2163
Size: 789 4564
MluI A'CGCG_T
Cuts at: 5229 5229
Size: 5353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4467 4651 1305
Size: 3162 184 2007
Fragments arranged by size:
3162 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2420 10
Size: 54 1425 343 588 2943
Fragments arranged by size:
2943 1425 588 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3311 3670 3829 518
Size: 648 2145 359 159 2042
Fragments arranged by size:
2145 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2237 2769 3446 4594 4839 1606
Size: 439 192 532 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 532 439 245 192
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3862
Fragments arranged by size:
3862 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5353
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5353
NotI GC'GGCC_GC
Cuts at: 2800 2800
Size: 5353
NspI r_CATG'y
Cuts at: 1132 1229 2711 4256 1132
Size: 97 1482 1545 2229
Fragments arranged by size:
2229 1545 1482 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5353
PciI A'CATG_T
Cuts at: 4252 4252
Size: 5353
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2048 2346 1108
Size: 940 298 4115
Fragments arranged by size:
4115 940 298
PfoI T'CCnGG_A
Cuts at: 2442 2610 2442
Size: 168 5185
PmeI GTTT'AAAC
Cuts at: 2657 2657
Size: 5353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 4935 4967 3422
Size: 32 1481 32 3808
Fragments arranged by size:
3808 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5353
PsiI TTA'TAA
Cuts at: 2923 2923
Size: 5353
PspOMI G'GGCC_C
Cuts at: 2283 2283
Size: 5353
PstI C_TGCA'G
Cuts at: 830 2648 2705 830
Size: 1818 57 3478
Fragments arranged by size:
3478 1818 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5353
PvuII CAG'CTG
Cuts at: 2045 2237 2045
Size: 192 5161
SalI G'TCGA_C
Cuts at: 1936 2695 1936
Size: 759 4594
SbfI CC_TGCA'GG
Cuts at: 2705 2705
Size: 5353
ScaI AGT'ACT
Cuts at: 3601 5313 3601
Size: 1712 3641
SfcI C'TryA_G
Cuts at: 826 1045 2360 2644 2701 3836 4517 4708 826
Size: 219 1315 284 57 1135 681 191 1471
Fragments arranged by size:
1471 1315 1135 681 284 219 191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5353
SmaI CCC'GGG
Cuts at: 1872 2680 1872
Size: 808 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3457 4358 4620 820
Size: 197 926 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3144 493
Size: 2651 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5353
SphI G_CATG'C
Cuts at: 1132 2711 1132
Size: 1579 3774
SspI AAT'ATT
Cuts at: 5 52 3277 5
Size: 47 3225 2081
Fragments arranged by size:
3225 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5353
StyI C'CwwG_G
Cuts at: 513 1065 2004 2792 513
Size: 552 939 788 3074
Fragments arranged by size:
3074 939 788 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 3380 3397 3550 3735 4180 325
Size: 1255 758 1042 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1042 758 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 3599 5311
Size: 274 80 33 51 1443 294 1328 1712
Cuts at: 5311 96
Size: 138
Fragments arranged by size:
1712 1443 1328 294 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5313 5345 5313
Size: 32 5321
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5353
XbaI T'CTAG_A
Cuts at: 2689 2689
Size: 5353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2573 2573
Size: 5353
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5353
XmaI C'CCGG_G
Cuts at: 1870 2678 1870
Size: 808 4545
XmnI GAAnn'nnTTC
Cuts at: 3482 3482
Size: 5353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3161 276
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI