Restriction map for
pFN21AB6965
(Circular) (Six-base) MAPSORT of: pFN21AB6965.seq Check: 1264 from: 1 to: 5224
pFN21AB6965 5224 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3034 278
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2513 2567 1937
Size: 576 54 4594
Fragments arranged by size:
4594 576 54
Acc65I G'GTAC_C
Cuts at: 2545 2545
Size: 5224
AclI AA'CG_TT
Cuts at: 1563 3351 3724 1563
Size: 1788 373 3063
Fragments arranged by size:
3063 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3287 4671 4939 1632
Size: 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5027
AflIII A'CryG_T
Cuts at: 1203 2475 4123 5100 1203
Size: 1272 1648 977 1327
Fragments arranged by size:
1648 1327 1272 977
AgeI A'CCGG_T
Cuts at: 4055 4055
Size: 5224
AhdI GACnn_n'nnGTC
Cuts at: 3953 3953
Size: 5224
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5224
AlwNI CAG_nnn'CTG
Cuts at: 2128 2967 4539 2128
Size: 839 1572 2813
Fragments arranged by size:
2813 1572 839
ApaLI G'TGCA_C
Cuts at: 3281 4437 3281
Size: 1156 4068
ApoI r'AATT_y
Cuts at: 1493 2533 2759 2911 5020 1493
Size: 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3778 160
Size: 872 2746 1606
Fragments arranged by size:
2746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2549 1870
Size: 73 253 353 4545
Fragments arranged by size:
4545 353 253 73
BamHI G'GATC_C
Cuts at: 2554 2921 2554
Size: 367 4857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2505 2545 4000 618
Size: 325 217 627 718 40 1455 1842
Fragments arranged by size:
1842 1455 718 627 325 217 40
BanII G_rGCy'C
Cuts at: 1391 2543 1391
Size: 1152 4072
BbeI G_GCGC'C
Cuts at: 2509 2509
Size: 5224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3415 3449 1903
Size: 34 1478 34 3678
Fragments arranged by size:
3678 1478 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4326 3116
Size: 1210 4014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 3835 136
Size: 107 122 71 631 605 526 1637 1525
Fragments arranged by size:
1637 1525 631 605 526 122 107 71
BglII A'GATC_T
Cuts at: 5219 5219
Size: 5224
BlpI GC'TnA_GC
Cuts at: 2641 2641
Size: 5224
Bme1580I G_kGCm'C
Cuts at: 1790 3285 4441 1790
Size: 1495 1156 2573
Fragments arranged by size:
2573 1495 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2955 3913 442
Size: 416 53 83 399 24 1538 958 1753
Fragments arranged by size:
1753 1538 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2385 3884 1239
Size: 1146 1499 2579
Fragments arranged by size:
2579 1499 1146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3349 4214 4512 3349
Size: 865 298 4061
Fragments arranged by size:
4061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3887 882
Size: 617 2388 2219
Fragments arranged by size:
2388 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3015 493
Size: 711 1811 2702
Fragments arranged by size:
2702 1811 711
BsaBI GATnn'nnATC
Cuts at: 5218 5218
Size: 5224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2506 3031 3413 4048
Size: 53 83 186 936 973 525 382 635
Cuts at: 4048 275
Size: 1451
Fragments arranged by size:
1451 973 936 635 525 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3657 4055 4329 4476 4887 3657
Size: 398 274 147 411 3994
Fragments arranged by size:
3994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2358 2388 1178
Size: 30 165 30 955 30 4014
Fragments arranged by size:
4014 955 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5224
BseYI C'CCAG_C
Cuts at: 2433 4427 4923 2433
Size: 1994 496 2734
Fragments arranged by size:
2734 1994 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4651
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2674 3435 4463 1540
Size: 275 186 673 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 275 186
BsiHKAI G_wGCw'C
Cuts at: 2448 3285 3370 4441 2448
Size: 837 85 1071 3231
Fragments arranged by size:
3231 1071 837 85
BsmI GAATG_Cn'
Cuts at: 2194 2504 2735 2828 2194
Size: 310 231 93 4590
Fragments arranged by size:
4590 310 231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2448 2543 3285 3370 4441 1391
Size: 399 658 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 658 399 95 85
BspEI T'CCGG_A
Cuts at: 4887 4887
Size: 5224
BspHI T'CATG_A
Cuts at: 2205 2448 3113 4843 2205
Size: 243 665 1730 2586
Fragments arranged by size:
2586 1730 665 243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2579 844
Size: 1325 410 3489
Fragments arranged by size:
3489 1325 410
BsrBI CCG'CTC
Cuts at: 1394 1823 3111 1394
Size: 429 1288 3507
Fragments arranged by size:
3507 1288 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3719 3893 65
Size: 1183 2471 174 1396
Fragments arranged by size:
2471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 2502 3868 4055 1700
Size: 110 287 405 1366 187 2869
Fragments arranged by size:
2869 1366 405 287 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 3313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5224
BssSI C'ACGA_G
Cuts at: 3284 4296 3284
Size: 1012 4212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2154 794
Size: 1360 3864
BstBI TT'CG_AA
Cuts at: 4028 4028
Size: 5224
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5224
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 2059
Size: 5224
BstYI r'GATC_y
Cuts at: 1972 2554 2921 3306 3323 4775 4861 4873 5219
Size: 582 367 385 17 1452 86 12 346
Cuts at: 5219 1972
Size: 1977
Fragments arranged by size:
1977 1452 582 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2070 513
Size: 552 1005 3667
Fragments arranged by size:
3667 1005 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2161 487
Size: 683 56 935 3550
Fragments arranged by size:
3550 935 683 56
BtsI GCAGTG_nn'
Cuts at: 2448 2740 3534 3554 2448
Size: 292 794 20 4118
Fragments arranged by size:
4118 794 292 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5189
DraI TTT'AAA
Cuts at: 2528 2528
Size: 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4231 809
Size: 507 2915 1802
Fragments arranged by size:
2915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2352 2671
Size: 54 1425 50 275 18 237 285 319
Cuts at: 2671 3560 5189 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 319 285 275 237 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2671 1537
Size: 275 859 4090
Fragments arranged by size:
4090 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3154 1353
Size: 1801 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3811 4185 4331 387
Size: 1064 138 2222 374 146 1280
Fragments arranged by size:
2222 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2385 3287 3884 4671 4939 1239
Size: 393 753 902 597 787 268 1524
Fragments arranged by size:
1524 902 787 753 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2109 2125 2109
Size: 16 5208
EcoRI G'AATT_C
Cuts at: 2533 2911 2533
Size: 378 4846
FspI TGC'GCA
Cuts at: 3730 3730
Size: 5224
HaeII r_GCGC'y
Cuts at: 1678 2410 2509 4371 1678
Size: 732 99 1862 2531
Fragments arranged by size:
2531 1862 732 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2358 2390 3865 3897 3939
Size: 32 135 32 786 32 1475 32 42
Cuts at: 3939 3971 1373
Size: 32 2626
Fragments arranged by size:
2626 1475 786 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 2814 669
Size: 988 281 630 246 3079
Fragments arranged by size:
3079 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5224
HpaI GTT'AAC
Cuts at: 2814 2814
Size: 5224
KasI G'GCGC_C
Cuts at: 2505 2505
Size: 5224
KpnI G_GTAC'C
Cuts at: 2549 2549
Size: 5224
MfeI C'AATT_G
Cuts at: 2823 2823
Size: 5224
MluI A'CGCG_T
Cuts at: 5100 5100
Size: 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4338 4522 1305
Size: 3033 184 2007
Fragments arranged by size:
3033 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 2354 10
Size: 54 1425 343 237 285 2880
Fragments arranged by size:
2880 1425 343 285 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 3182 3541 3700 518
Size: 648 1077 939 359 159 2042
Fragments arranged by size:
2042 1077 939 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2640 3317 4465 4710 1606
Size: 1034 677 1148 245 2120
Fragments arranged by size:
2120 1148 1034 677 245
NaeI GCC'GGC
Cuts at: 2504 2504
Size: 5224
NarI GG'CG_CC
Cuts at: 2506 2506
Size: 5224
NcoI C'CATG_G
Cuts at: 513 1065 2070 513
Size: 552 1005 3667
Fragments arranged by size:
3667 1005 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5224
NgoMIV G'CCGG_C
Cuts at: 2502 2502
Size: 5224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5224
NotI GC'GGCC_GC
Cuts at: 2671 2671
Size: 5224
NspI r_CATG'y
Cuts at: 1132 1229 2479 2582 4127 1132
Size: 97 1250 103 1545 2229
Fragments arranged by size:
2229 1545 1250 103 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5224
PciI A'CATG_T
Cuts at: 2475 4123 2475
Size: 1648 3576
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5224
PmeI GTTT'AAAC
Cuts at: 2528 2528
Size: 5224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 4806 4838 3293
Size: 32 1481 32 3679
Fragments arranged by size:
3679 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2109 2109
Size: 5224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5224
PsiI TTA'TAA
Cuts at: 2794 2794
Size: 5224
PstI C_TGCA'G
Cuts at: 830 2166 2576 830
Size: 1336 410 3478
Fragments arranged by size:
3478 1336 410
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5224
SalI G'TCGA_C
Cuts at: 1936 2566 1936
Size: 630 4594
SbfI CC_TGCA'GG
Cuts at: 2576 2576
Size: 5224
ScaI AGT'ACT
Cuts at: 3472 5184 3472
Size: 1712 3512
SfcI C'TryA_G
Cuts at: 826 1045 2162 2572 3707 4388 4579 826
Size: 219 1117 410 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 681 410 219 191
SfoI GGC'GCC
Cuts at: 2507 2507
Size: 5224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5224
SmaI CCC'GGG
Cuts at: 1872 2198 2551 1872
Size: 326 353 4545
Fragments arranged by size:
4545 353 326
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3328 4229 4491 820
Size: 197 926 1385 901 262 1553
Fragments arranged by size:
1553 1385 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3015 493
Size: 2522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5224
SphI G_CATG'C
Cuts at: 1132 2582 1132
Size: 1450 3774
SspI AAT'ATT
Cuts at: 5 52 3148 5
Size: 47 3096 2081
Fragments arranged by size:
3096 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5224
StyI C'CwwG_G
Cuts at: 513 1065 2070 2349 2663 513
Size: 552 1005 279 314 3074
Fragments arranged by size:
3074 1005 552 314 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2009 2032 2425 3251 3268 3421 3606
Size: 1255 429 23 393 826 17 153 185
Cuts at: 3606 4051 325
Size: 445 1498
Fragments arranged by size:
1498 1255 826 445 429 393 185 153
23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3470 5182
Size: 274 80 33 51 1443 30 1463 1712
Cuts at: 5182 96
Size: 138
Fragments arranged by size:
1712 1463 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5184 5216 5184
Size: 32 5192
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5224
XbaI T'CTAG_A
Cuts at: 2560 2560
Size: 5224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 5224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5224
XmaI C'CCGG_G
Cuts at: 1870 2196 2549 1870
Size: 326 353 4545
Fragments arranged by size:
4545 353 326
XmnI GAAnn'nnTTC
Cuts at: 3353 3353
Size: 5224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3032 276
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI