Restriction map for
pFN21AB6956
(Circular) (Six-base) MAPSORT of: pFN21AB6956.seq Check: 3889 from: 1 to: 5419
pFN21AB6956 5419 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2296 2296
Size: 5419
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2299 3229 278
Size: 53 83 186 936 763 930 2468
Fragments arranged by size:
2468 936 930 763 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2762 1937
Size: 825 4594
Acc65I G'GTAC_C
Cuts at: 2740 2740
Size: 5419
AclI AA'CG_TT
Cuts at: 1563 3546 3919 1563
Size: 1983 373 3063
Fragments arranged by size:
3063 1983 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3482 4866 5134 1632
Size: 1850 1384 268 1917
Fragments arranged by size:
1917 1850 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5222
AflIII A'CryG_T
Cuts at: 1203 4318 5295 1203
Size: 3115 977 1327
Fragments arranged by size:
3115 1327 977
AgeI A'CCGG_T
Cuts at: 4250 4250
Size: 5419
AhdI GACnn_n'nnGTC
Cuts at: 4148 4148
Size: 5419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5419
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5419
AlwNI CAG_nnn'CTG
Cuts at: 3162 4734 3162
Size: 1572 3847
ApaLI G'TGCA_C
Cuts at: 3476 4632 3476
Size: 1156 4263
ApoI r'AATT_y
Cuts at: 1493 2162 2728 2954 3106 5215 1493
Size: 669 566 226 152 2109 1697
Fragments arranged by size:
2109 1697 669 566 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3973 160
Size: 872 2941 1606
Fragments arranged by size:
2941 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 2744 1870
Size: 73 286 515 4545
Fragments arranged by size:
4545 515 286 73
BamHI G'GATC_C
Cuts at: 2749 3116 2749
Size: 367 5052
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2740 4195 618
Size: 325 217 627 953 1455 1842
Fragments arranged by size:
1842 1455 953 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2738 1391
Size: 1347 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2239 2273 3610 3644 1903
Size: 34 302 34 1337 34 3678
Fragments arranged by size:
3678 1337 302 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3311 4521 3311
Size: 1210 4209
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5419
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4030 136
Size: 107 122 71 631 605 2358 1525
Fragments arranged by size:
2358 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2495 2537 2645 5414 2495
Size: 42 108 2769 2500
Fragments arranged by size:
2769 2500 108 42
BlpI GC'TnA_GC
Cuts at: 2836 2836
Size: 5419
Bme1580I G_kGCm'C
Cuts at: 1790 3480 4636 1790
Size: 1690 1156 2573
Fragments arranged by size:
2573 1690 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3150 4108 442
Size: 416 53 83 399 24 1733 958 1753
Fragments arranged by size:
1753 1733 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5419
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2157 2157
Size: 5419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 4079 1239
Size: 888 1952 2579
Fragments arranged by size:
2579 1952 888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3544 4409 4707 3544
Size: 865 298 4256
Fragments arranged by size:
4256 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4082 882
Size: 617 2583 2219
Fragments arranged by size:
2583 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3210 493
Size: 711 2006 2702
Fragments arranged by size:
2702 2006 711
BsaBI GATnn'nnATC
Cuts at: 2361 2494 2518 2599 2650 5413 2361
Size: 133 24 81 51 2763 2367
Fragments arranged by size:
2763 2367 133 81 51 24
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2296 3226 3608 4243
Size: 53 83 186 936 763 930 382 635
Cuts at: 4243 275
Size: 1451
Fragments arranged by size:
1451 936 930 763 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3852 4250 4524 4671 5082 3852
Size: 398 274 147 411 4189
Fragments arranged by size:
4189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2257 2287 1178
Size: 30 165 30 854 30 4310
Fragments arranged by size:
4310 854 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2043 2472 1186
Size: 857 429 4133
Fragments arranged by size:
4133 857 429
BseYI C'CCAG_C
Cuts at: 4622 5118 4622
Size: 496 4923
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4846
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2869 3630 4658 1540
Size: 275 186 868 761 1028 2301
Fragments arranged by size:
2301 1028 868 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3480 3565 4636 3480
Size: 85 1071 4263
Fragments arranged by size:
4263 1071 85
BsmI GAATG_Cn'
Cuts at: 2930 3023 2930
Size: 93 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2738 3480 3565 4636 1391
Size: 399 948 742 85 1071 2174
Fragments arranged by size:
2174 1071 948 742 399 85
BspEI T'CCGG_A
Cuts at: 5082 5082
Size: 5419
BspHI T'CATG_A
Cuts at: 3308 5038 3308
Size: 1730 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2296 2379 2774 844
Size: 1452 83 395 3489
Fragments arranged by size:
3489 1452 395 83
BsrBI CCG'CTC
Cuts at: 1394 1823 3306 1394
Size: 429 1483 3507
Fragments arranged by size:
3507 1483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3914 4088 65
Size: 1183 2666 174 1396
Fragments arranged by size:
2666 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2373 4063 4250 1700
Size: 110 563 1690 187 2869
Fragments arranged by size:
2869 1690 563 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5419
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5419
BssSI C'ACGA_G
Cuts at: 3479 4491 3479
Size: 1012 4407
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2189 794
Size: 1395 4024
BstBI TT'CG_AA
Cuts at: 2166 4223 2166
Size: 2057 3362
BstEII G'GTnAC_C
Cuts at: 1184 2051 1184
Size: 867 4552
BstYI r'GATC_y
Cuts at: 1972 2170 2495 2537 2600 2645 2749 3116 3501
Size: 198 325 42 63 45 104 367 385
Cuts at: 3501 3518 4970 5056 5068 5414 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 325 198 104
86 63 45 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4867
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4680
Fragments arranged by size:
4680 683 56
BtsI GCAGTG_nn'
Cuts at: 2935 3729 3749 2935
Size: 794 20 4605
Fragments arranged by size:
4605 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5384
DraI TTT'AAA
Cuts at: 2723 2723
Size: 5419
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4426 809
Size: 507 3110 1802
Fragments arranged by size:
3110 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2866 3755 5384
Size: 54 1425 50 275 18 1036 889 1629
Cuts at: 5384 8
Size: 43
Fragments arranged by size:
1629 1425 1036 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2866 1537
Size: 275 1054 4090
Fragments arranged by size:
4090 1054 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2511 3349 1353
Size: 1158 838 3423
Fragments arranged by size:
3423 1158 838
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4006 4380 4526 387
Size: 1064 138 2417 374 146 1280
Fragments arranged by size:
2417 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 3482 4079 4866 5134 1239
Size: 393 495 1355 597 787 268 1524
Fragments arranged by size:
1524 1355 787 597 495 393 268
EcoRI G'AATT_C
Cuts at: 2162 2728 3106 2162
Size: 566 378 4475
Fragments arranged by size:
4475 566 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2541 2541
Size: 5419
FspI TGC'GCA
Cuts at: 3925 3925
Size: 5419
HaeII r_GCGC'y
Cuts at: 1678 2070 4566 1678
Size: 392 2496 2531
Fragments arranged by size:
2531 2496 392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4060 4092 4134 4166 1373
Size: 32 135 32 2488 32 42 32 2626
Fragments arranged by size:
2626 2488 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2763 3009 669
Size: 988 281 825 246 3079
Fragments arranged by size:
3079 988 825 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5419
HpaI GTT'AAC
Cuts at: 3009 3009
Size: 5419
KpnI G_GTAC'C
Cuts at: 2744 2744
Size: 5419
MfeI C'AATT_G
Cuts at: 3018 3018
Size: 5419
MluI A'CGCG_T
Cuts at: 5295 5295
Size: 5419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4533 4717 1305
Size: 3228 184 2007
Fragments arranged by size:
3228 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3597
Fragments arranged by size:
3597 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3377 3736 3895 518
Size: 648 2211 359 159 2042
Fragments arranged by size:
2211 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2835 3512 4660 4905 1606
Size: 1229 677 1148 245 2120
Fragments arranged by size:
2120 1229 1148 677 245
NaeI GCC'GGC
Cuts at: 2375 2375
Size: 5419
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4867
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5419
NgoMIV G'CCGG_C
Cuts at: 2373 2373
Size: 5419
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5419
NotI GC'GGCC_GC
Cuts at: 2866 2866
Size: 5419
NspI r_CATG'y
Cuts at: 1132 1229 2260 2777 4322 1132
Size: 97 1031 517 1545 2229
Fragments arranged by size:
2229 1545 1031 517 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5419
PciI A'CATG_T
Cuts at: 4318 4318
Size: 5419
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5419
PfoI T'CCnGG_A
Cuts at: 2143 2143
Size: 5419
PmeI GTTT'AAAC
Cuts at: 2723 2723
Size: 5419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 5001 5033 3488
Size: 32 1481 32 3874
Fragments arranged by size:
3874 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2464 1613
Size: 851 4568
PsiI TTA'TAA
Cuts at: 2989 2989
Size: 5419
PstI C_TGCA'G
Cuts at: 830 2771 830
Size: 1941 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5419
SalI G'TCGA_C
Cuts at: 1936 2761 1936
Size: 825 4594
SbfI CC_TGCA'GG
Cuts at: 2771 2771
Size: 5419
ScaI AGT'ACT
Cuts at: 3667 5379 3667
Size: 1712 3707
SfcI C'TryA_G
Cuts at: 826 1045 2767 3902 4583 4774 826
Size: 219 1722 1135 681 191 1471
Fragments arranged by size:
1722 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5419
SmaI CCC'GGG
Cuts at: 1872 2746 1872
Size: 874 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3523 4424 4686 820
Size: 197 926 1580 901 262 1553
Fragments arranged by size:
1580 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3210 493
Size: 2717 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5419
SphI G_CATG'C
Cuts at: 1132 2260 2777 1132
Size: 1128 517 3774
Fragments arranged by size:
3774 1128 517
SspI AAT'ATT
Cuts at: 5 52 3343 5
Size: 47 3291 2081
Fragments arranged by size:
3291 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5419
StyI C'CwwG_G
Cuts at: 513 1065 2037 2858 513
Size: 552 972 821 3074
Fragments arranged by size:
3074 972 821 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3446 3463 3616 3801 4246 325
Size: 1255 1866 17 153 185 445 1498
Fragments arranged by size:
1866 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3665 5377 96
Size: 274 80 33 51 1443 1688 1712 138
Fragments arranged by size:
1712 1688 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5379 5411 5379
Size: 32 5387
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5419
XbaI T'CTAG_A
Cuts at: 2399 2755 2399
Size: 356 5063
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5419
XmaI C'CCGG_G
Cuts at: 1870 2744 1870
Size: 874 4545
XmnI GAAnn'nnTTC
Cuts at: 2668 3548 2668
Size: 880 4539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2297 3227 276
Size: 53 83 186 936 763 930 2468
Fragments arranged by size:
2468 936 930 763 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI