Restriction map for
pFN21AB6942
(Circular) (Six-base) MAPSORT of: pFN21AB6942.seq Check: 6441 from: 1 to: 5392
pFN21AB6942 5392 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2152
Size: 5392
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3202 278
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2735 1937
Size: 798 4594
Acc65I G'GTAC_C
Cuts at: 2713 2713
Size: 5392
AclI AA'CG_TT
Cuts at: 1563 3519 3892 1563
Size: 1956 373 3063
Fragments arranged by size:
3063 1956 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 3455 4839 5107 1632
Size: 1058 765 1384 268 1917
Fragments arranged by size:
1917 1384 1058 765 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5195
AflIII A'CryG_T
Cuts at: 1203 4291 5268 1203
Size: 3088 977 1327
Fragments arranged by size:
3088 1327 977
AgeI A'CCGG_T
Cuts at: 4223 4223
Size: 5392
AhdI GACnn_n'nnGTC
Cuts at: 4121 4121
Size: 5392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2517 1184
Size: 1333 4059
AlwNI CAG_nnn'CTG
Cuts at: 2504 3135 4707 2504
Size: 631 1572 3189
Fragments arranged by size:
3189 1572 631
ApaI G_GGCC'C
Cuts at: 2180 2180
Size: 5392
ApaLI G'TGCA_C
Cuts at: 3449 4605 3449
Size: 1156 4236
ApoI r'AATT_y
Cuts at: 1493 2701 2927 3079 5188 1493
Size: 1208 226 152 2109 1697
Fragments arranged by size:
2109 1697 1208 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3946 160
Size: 872 2914 1606
Fragments arranged by size:
2914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
AvaI C'yCGr_G
Cuts at: 1870 1943 2717 1870
Size: 73 774 4545
Fragments arranged by size:
4545 774 73
BamHI G'GATC_C
Cuts at: 2722 3089 2722
Size: 367 5025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2140 2713 4168 618
Size: 325 217 627 258 95 573 1455 1842
Fragments arranged by size:
1842 1455 627 573 325 258 217 95
BanII G_rGCy'C
Cuts at: 1391 2180 2711 1391
Size: 789 531 4072
Fragments arranged by size:
4072 789 531
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3583 3617 1903
Size: 34 1646 34 3678
Fragments arranged by size:
3678 1646 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3284 4494 3284
Size: 1210 4182
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5392
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 2087 4003
Size: 107 122 71 631 605 382 33 1916
Cuts at: 4003 136
Size: 1525
Fragments arranged by size:
1916 1525 631 605 382 122 107 71
33
BglII A'GATC_T
Cuts at: 5387 5387
Size: 5392
BlpI GC'TnA_GC
Cuts at: 2809 2809
Size: 5392
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2143 2180 3453 4609 1790
Size: 260 93 37 1273 1156 2573
Fragments arranged by size:
2573 1273 1156 260 93 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5392
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 4081 442
Size: 416 53 83 399 24 1706 958 1753
Fragments arranged by size:
1753 1706 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 4052 1239
Size: 1193 1620 2579
Fragments arranged by size:
2579 1620 1193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3517 4382 4680 3517
Size: 865 298 4229
Fragments arranged by size:
4229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2555 4055 882
Size: 617 1056 1500 2219
Fragments arranged by size:
2219 1500 1056 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 3183 493
Size: 711 1317 662 2702
Fragments arranged by size:
2702 1317 711 662
BsaBI GATnn'nnATC
Cuts at: 5386 5386
Size: 5392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2678 3199 3581 4216
Size: 53 83 186 936 1145 521 382 635
Cuts at: 4216 275
Size: 1451
Fragments arranged by size:
1451 1145 936 635 521 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2466 3825 4223 4497 4644 5055 2466
Size: 1359 398 274 147 411 2803
Fragments arranged by size:
2803 1359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5167
Fragments arranged by size:
5167 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2342 2429 2546 2573 1186
Size: 892 264 87 117 27 4005
Fragments arranged by size:
4005 892 264 117 87 27
BseYI C'CCAG_C
Cuts at: 2091 2592 4595 5091 2091
Size: 501 2003 496 2392
Fragments arranged by size:
2392 2003 501 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4819
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2842 3603 4631 1540
Size: 275 186 78 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 2661 3453 3538 4609 2661
Size: 792 85 1071 3444
Fragments arranged by size:
3444 1071 792 85
BsmI GAATG_Cn'
Cuts at: 2483 2903 2996 2483
Size: 420 93 4879
Fragments arranged by size:
4879 420 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2143 2180 2661 2711 3453 3538
Size: 399 260 93 37 481 50 742 85
Cuts at: 3538 4609 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 481 399 260 93 85
50 37
BspEI T'CCGG_A
Cuts at: 5055 5055
Size: 5392
BspHI T'CATG_A
Cuts at: 3281 5011 3281
Size: 1730 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 2747 844
Size: 1308 595 3489
Fragments arranged by size:
3489 1308 595
BsrBI CCG'CTC
Cuts at: 1394 1823 2043 3279 1394
Size: 429 220 1236 3507
Fragments arranged by size:
3507 1236 429 220
BsrDI GCAATG_nn'
Cuts at: 65 1248 2271 3887 4061 65
Size: 1183 1023 1616 174 1396
Fragments arranged by size:
1616 1396 1183 1023 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 4036 4223 1700
Size: 110 337 1889 187 2869
Fragments arranged by size:
2869 1889 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5392
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5392
BssSI C'ACGA_G
Cuts at: 2182 3452 4464 2182
Size: 1270 1012 3110
Fragments arranged by size:
3110 1270 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2273 2628 794
Size: 1479 355 3558
Fragments arranged by size:
3558 1479 355
BstBI TT'CG_AA
Cuts at: 4196 4196
Size: 5392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5392
BstYI r'GATC_y
Cuts at: 1972 2722 3089 3474 3491 4943 5029 5041 5387
Size: 750 367 385 17 1452 86 12 346
Cuts at: 5387 1972
Size: 1977
Fragments arranged by size:
1977 1452 750 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3901
Fragments arranged by size:
3901 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4653
Fragments arranged by size:
4653 683 56
BtsI GCAGTG_nn'
Cuts at: 2633 2908 3702 3722 2633
Size: 275 794 20 4303
Fragments arranged by size:
4303 794 275 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5357
DraI TTT'AAA
Cuts at: 2696 2696
Size: 5392
DraIII CAC_nnn'GTG
Cuts at: 2577 2577
Size: 5392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2683 4399 809
Size: 507 1367 1716 1802
Fragments arranged by size:
1802 1716 1367 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2839 3728
Size: 54 1425 50 275 18 246 763 889
Cuts at: 3728 5357 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 763 275 246 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2076 2839 1537
Size: 275 264 763 4090
Fragments arranged by size:
4090 763 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 3322 1353
Size: 1969 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 3979 4353 4499 387
Size: 1064 138 486 1904 374 146 1280
Fragments arranged by size:
1904 1280 1064 486 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 2690 3455 4052 4839 5107 1239
Size: 393 800 258 765 597 787 268 1524
Fragments arranged by size:
1524 800 787 765 597 393 268 258
EcoO109I rG'GnC_Cy
Cuts at: 2068 2177 2407 2068
Size: 109 230 5053
Fragments arranged by size:
5053 230 109
EcoRI G'AATT_C
Cuts at: 2701 3079 2701
Size: 378 5014
EcoRV GAT'ATC
Cuts at: 2509 2509
Size: 5392
FspI TGC'GCA
Cuts at: 3898 3898
Size: 5392
HaeII r_GCGC'y
Cuts at: 1678 2431 4539 1678
Size: 753 2108 2531
Fragments arranged by size:
2531 2108 753
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 4033 4065 4107
Size: 32 135 32 776 32 1653 32 42
Cuts at: 4107 4139 1373
Size: 32 2626
Fragments arranged by size:
2626 1653 776 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2982 669
Size: 988 281 798 246 3079
Fragments arranged by size:
3079 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5392
HpaI GTT'AAC
Cuts at: 2982 2982
Size: 5392
KpnI G_GTAC'C
Cuts at: 2717 2717
Size: 5392
MfeI C'AATT_G
Cuts at: 2991 2991
Size: 5392
MluI A'CGCG_T
Cuts at: 5268 5268
Size: 5392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2016 4506 4690 1305
Size: 711 2490 184 2007
Fragments arranged by size:
2490 2007 711 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3570
Fragments arranged by size:
3570 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3350 3709 3868 518
Size: 648 2184 359 159 2042
Fragments arranged by size:
2184 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2808 3485 4633 4878 1606
Size: 1202 677 1148 245 2120
Fragments arranged by size:
2120 1202 1148 677 245
NaeI GCC'GGC
Cuts at: 2149 2149
Size: 5392
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3901
Fragments arranged by size:
3901 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5392
NgoMIV G'CCGG_C
Cuts at: 2147 2147
Size: 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5392
NotI GC'GGCC_GC
Cuts at: 2076 2839 2076
Size: 763 4629
NspI r_CATG'y
Cuts at: 1132 1229 2750 4295 1132
Size: 97 1521 1545 2229
Fragments arranged by size:
2229 1545 1521 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5392
PciI A'CATG_T
Cuts at: 4291 4291
Size: 5392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5392
PmeI GTTT'AAAC
Cuts at: 2696 2696
Size: 5392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 4974 5006 3461
Size: 32 1481 32 3847
Fragments arranged by size:
3847 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2407 2068
Size: 339 5053
PshAI GACnn'nnGTC
Cuts at: 1613 2216 1613
Size: 603 4789
PsiI TTA'TAA
Cuts at: 2962 2962
Size: 5392
PspOMI G'GGCC_C
Cuts at: 2176 2176
Size: 5392
PstI C_TGCA'G
Cuts at: 830 2744 830
Size: 1914 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5392
SalI G'TCGA_C
Cuts at: 1936 2734 1936
Size: 798 4594
SbfI CC_TGCA'GG
Cuts at: 2744 2744
Size: 5392
ScaI AGT'ACT
Cuts at: 3640 5352 3640
Size: 1712 3680
SfcI C'TryA_G
Cuts at: 826 1045 2740 3875 4556 4747 826
Size: 219 1695 1135 681 191 1471
Fragments arranged by size:
1695 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5392
SmaI CCC'GGG
Cuts at: 1872 2719 1872
Size: 847 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3496 4397 4659 820
Size: 197 926 1553 901 262 1553
Fragments arranged by size:
1553 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3183 493
Size: 2690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5392
SphI G_CATG'C
Cuts at: 1132 2750 1132
Size: 1618 3774
SspI AAT'ATT
Cuts at: 5 52 3316 5
Size: 47 3264 2081
Fragments arranged by size:
3264 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5392
StyI C'CwwG_G
Cuts at: 513 1065 2004 2553 2831 513
Size: 552 939 549 278 3074
Fragments arranged by size:
3074 939 552 549 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3419 3436 3589 3774 4219 325
Size: 1255 1839 17 153 185 445 1498
Fragments arranged by size:
1839 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3638 5350 96
Size: 274 80 33 51 1443 1661 1712 138
Fragments arranged by size:
1712 1661 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5352 5384 5352
Size: 32 5360
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5392
XbaI T'CTAG_A
Cuts at: 2728 2728
Size: 5392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2407 2574 2407
Size: 167 5225
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5392
XmaI C'CCGG_G
Cuts at: 1870 2717 1870
Size: 847 4545
XmnI GAAnn'nnTTC
Cuts at: 3521 3521
Size: 5392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3200 276
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI