Restriction map for
pFN21AB6935
(Circular) (Six-base) MAPSORT of: pFN21AB6935.seq Check: 6296 from: 1 to: 5326
pFN21AB6935 5326 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2120 3136 278
Size: 53 83 186 936 584 1016 2468
Fragments arranged by size:
2468 1016 936 584 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 1937
Size: 732 4594
Acc65I G'GTAC_C
Cuts at: 2647 2647
Size: 5326
AclI AA'CG_TT
Cuts at: 1563 2625 3453 3826 1563
Size: 1062 828 373 3063
Fragments arranged by size:
3063 1062 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 3389 4773 5041 1632
Size: 701 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1056 701 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 4225 5202 1203
Size: 3022 977 1327
Fragments arranged by size:
3022 1327 977
AgeI A'CCGG_T
Cuts at: 2526 4157 2526
Size: 1631 3695
AhdI GACnn_n'nnGTC
Cuts at: 4055 4055
Size: 5326
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5326
AlwNI CAG_nnn'CTG
Cuts at: 2086 3069 4641 2086
Size: 983 1572 2771
Fragments arranged by size:
2771 1572 983
ApaLI G'TGCA_C
Cuts at: 2188 3383 4539 2188
Size: 1195 1156 2975
Fragments arranged by size:
2975 1195 1156
ApoI r'AATT_y
Cuts at: 1493 2324 2635 2861 3013 5122 1493
Size: 831 311 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 311 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3880 160
Size: 872 2848 1606
Fragments arranged by size:
2848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 2599 2651 1870
Size: 73 279 377 52 4545
Fragments arranged by size:
4545 377 279 73 52
BamHI G'GATC_C
Cuts at: 2656 3023 2656
Size: 367 4959
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2647 4102 618
Size: 325 217 627 860 1455 1842
Fragments arranged by size:
1842 1455 860 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2178 2558 2606 2645 1391
Size: 787 380 48 39 4072
Fragments arranged by size:
4072 787 380 48 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 1903
Size: 34 1580 34 3678
Fragments arranged by size:
3678 1580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3218 4428 3218
Size: 1210 4116
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3937 136
Size: 107 122 71 631 605 2265 1525
Fragments arranged by size:
2265 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5321 5321
Size: 5326
BlpI GC'TnA_GC
Cuts at: 2743 2743
Size: 5326
Bme1580I G_kGCm'C
Cuts at: 1790 2192 3387 4543 1790
Size: 402 1195 1156 2573
Fragments arranged by size:
2573 1195 1156 402
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3057 4015 442
Size: 416 53 83 399 24 1640 958 1753
Fragments arranged by size:
1753 1640 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 3986 1239
Size: 842 1905 2579
Fragments arranged by size:
2579 1905 842
Bpu10I CC'TnA_GC
Cuts at: 2557 2557
Size: 5326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 3451 4316 4614 2564
Size: 887 865 298 3276
Fragments arranged by size:
3276 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 882
Size: 617 2490 2219
Fragments arranged by size:
2490 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3117 493
Size: 711 1913 2702
Fragments arranged by size:
2702 1913 711
BsaBI GATnn'nnATC
Cuts at: 5320 5320
Size: 5326
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2117 3133 3515 4150
Size: 53 83 186 936 584 1016 382 635
Cuts at: 4150 275
Size: 1451
Fragments arranged by size:
1451 1016 936 635 584 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2170 2480 2526 3759 4157 4431 4578 4989 2170
Size: 310 46 1233 398 274 147 411 2507
Fragments arranged by size:
2507 1233 411 398 310 274 147 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2545 2575 1178
Size: 30 165 30 1142 30 3929
Fragments arranged by size:
3929 1142 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2412 1186
Size: 1226 4100
BseYI C'CCAG_C
Cuts at: 2143 4529 5025 2143
Size: 2386 496 2444
Fragments arranged by size:
2444 2386 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 1163
Size: 573 336 4417
Fragments arranged by size:
4417 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2776 3537 4565 1540
Size: 275 186 775 761 1028 2301
Fragments arranged by size:
2301 1028 775 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2192 2558 3387 3472 4543 2192
Size: 366 829 85 1071 2975
Fragments arranged by size:
2975 1071 829 366 85
BsmI GAATG_Cn'
Cuts at: 2209 2837 2930 2209
Size: 628 93 4605
Fragments arranged by size:
4605 628 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2192 2558 2606 2645 3387 3472
Size: 399 388 14 366 48 39 742 85
Cuts at: 3472 4543 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 366 85 48
39 14
BspEI T'CCGG_A
Cuts at: 2170 2480 4989 2170
Size: 310 2509 2507
Fragments arranged by size:
2509 2507 310
BspHI T'CATG_A
Cuts at: 3215 4945 3215
Size: 1730 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2681 844
Size: 1837 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2588 3213 1394
Size: 429 765 625 3507
Fragments arranged by size:
3507 765 625 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2409 3821 3995 65
Size: 1183 1161 1412 174 1396
Fragments arranged by size:
1412 1396 1183 1161 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2248 2526 3970 4157 1700
Size: 110 438 278 1444 187 2869
Fragments arranged by size:
2869 1444 438 278 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 3386 4398 3386
Size: 1012 4314
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2086 2129 794
Size: 1292 43 3991
Fragments arranged by size:
3991 1292 43
BstBI TT'CG_AA
Cuts at: 4130 4130
Size: 5326
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5326
BstYI r'GATC_y
Cuts at: 1972 2656 3023 3408 3425 4877 4963 4975 5321
Size: 684 367 385 17 1452 86 12 346
Cuts at: 5321 1972
Size: 1977
Fragments arranged by size:
1977 1452 684 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 2466 513
Size: 552 939 129 333 3373
Fragments arranged by size:
3373 939 552 333 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4587
Fragments arranged by size:
4587 683 56
BtsI GCAGTG_nn'
Cuts at: 2192 2397 2842 3636 3656 2192
Size: 205 445 794 20 3862
Fragments arranged by size:
3862 794 445 205 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2630 2630
Size: 5326
DraIII CAC_nnn'GTG
Cuts at: 2446 2446
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 809
Size: 507 3017 1802
Fragments arranged by size:
3017 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 2617 2773
Size: 54 1425 50 275 18 660 127 156
Cuts at: 2773 3662 5291 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 660 275 156 127 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2773 1537
Size: 275 961 4090
Fragments arranged by size:
4090 961 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 2259 3256 1353
Size: 876 30 997 3423
Fragments arranged by size:
3423 997 876 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3913 4287 4433 387
Size: 1064 138 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2556 2556
Size: 5326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2333 3389 3986 4773 5041 1239
Size: 393 449 252 1056 597 787 268 1524
Fragments arranged by size:
1524 1056 787 597 449 393 268 252
EcoNI CCTnn'n_nnAGG
Cuts at: 2108 2108
Size: 5326
EcoO109I rG'GnC_Cy
Cuts at: 2140 2571 2140
Size: 431 4895
EcoRI G'AATT_C
Cuts at: 2635 3013 2635
Size: 378 4948
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 5326
FspI TGC'GCA
Cuts at: 3832 3832
Size: 5326
HaeII r_GCGC'y
Cuts at: 1678 4473 1678
Size: 2795 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 2545 2577 3967
Size: 32 135 32 826 32 115 32 1390
Cuts at: 3967 3999 4041 4073 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1390 826 135 115 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KpnI G_GTAC'C
Cuts at: 2651 2651
Size: 5326
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4440 4624 1305
Size: 3135 184 2007
Fragments arranged by size:
3135 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3504
Fragments arranged by size:
3504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3284 3643 3802 518
Size: 648 2118 359 159 2042
Fragments arranged by size:
2118 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2126 2153 2460 2742 3419 4567 4812 1606
Size: 520 27 307 282 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 520 307 282 245 27
NaeI GCC'GGC
Cuts at: 2250 2250
Size: 5326
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3835
Fragments arranged by size:
3835 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5326
NgoMIV G'CCGG_C
Cuts at: 2248 2248
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2773 2773
Size: 5326
NspI r_CATG'y
Cuts at: 1132 1229 2684 4229 1132
Size: 97 1455 1545 2229
Fragments arranged by size:
2229 1545 1455 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5326
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2446 2530 1108
Size: 1338 84 3904
Fragments arranged by size:
3904 1338 84
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 4908 4940 3395
Size: 32 1481 32 3781
Fragments arranged by size:
3781 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2140
Size: 5326
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5326
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PstI C_TGCA'G
Cuts at: 830 2125 2456 2678 830
Size: 1295 331 222 3478
Fragments arranged by size:
3478 1295 331 222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
PvuII CAG'CTG
Cuts at: 2126 2126
Size: 5326
SacI G_AGCT'C
Cuts at: 2558 2558
Size: 5326
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SapI GCTCTTCn'nnn_
Cuts at: 2259 2259
Size: 5326
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 3574 5286 3574
Size: 1712 3614
SfcI C'TryA_G
Cuts at: 826 1045 2121 2452 2674 3809 4490 4681 826
Size: 219 1076 331 222 1135 681 191 1471
Fragments arranged by size:
1471 1135 1076 681 331 222 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2653 1872
Size: 781 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 3430 4331 4593 820
Size: 197 926 636 851 901 262 1553
Fragments arranged by size:
1553 926 901 851 636 262 197
SnaBI TAC'GTA
Cuts at: 493 3117 493
Size: 2624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2684 1132
Size: 1552 3774
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5326
StyI C'CwwG_G
Cuts at: 513 1065 2004 2765 513
Size: 552 939 761 3074
Fragments arranged by size:
3074 939 761 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3353 3370 3523 3708 4153 325
Size: 1255 1773 17 153 185 445 1498
Fragments arranged by size:
1773 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3572 5284 96
Size: 274 80 33 51 1443 1595 1712 138
Fragments arranged by size:
1712 1595 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2478 2510 5286 5318 2478
Size: 32 2776 32 2486
Fragments arranged by size:
2776 2486 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5326
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5326
XmaI C'CCGG_G
Cuts at: 1870 2651 1870
Size: 781 4545
XmnI GAAnn'nnTTC
Cuts at: 2505 3455 2505
Size: 950 4376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2118 3134 276
Size: 53 83 186 936 584 1016 2468
Fragments arranged by size:
2468 1016 936 584 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI