Restriction map for
pFN21AB6931
(Circular) (Six-base) MAPSORT of: pFN21AB6931.seq Check: 4729 from: 1 to: 5326
pFN21AB6931 5326 bp
With 182 enzymes: SgfI *
March 28, 2012 13:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2346 3136 278
Size: 53 83 186 936 810 790 2468
Fragments arranged by size:
2468 936 810 790 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 2669 1937
Size: 328 404 4594
Fragments arranged by size:
4594 404 328
Acc65I G'GTAC_C
Cuts at: 2647 2647
Size: 5326
AclI AA'CG_TT
Cuts at: 1563 3453 3826 1563
Size: 1890 373 3063
Fragments arranged by size:
3063 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3389 4773 5041 1632
Size: 1757 1384 268 1917
Fragments arranged by size:
1917 1757 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 4225 5202 1203
Size: 3022 977 1327
Fragments arranged by size:
3022 1327 977
AgeI A'CCGG_T
Cuts at: 4157 4157
Size: 5326
AhdI GACnn_n'nnGTC
Cuts at: 4055 4055
Size: 5326
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2352 1184
Size: 1168 4158
AlwNI CAG_nnn'CTG
Cuts at: 3069 4641 3069
Size: 1572 3754
ApaLI G'TGCA_C
Cuts at: 3383 4539 3383
Size: 1156 4170
ApoI r'AATT_y
Cuts at: 1493 2635 2861 3013 5122 1493
Size: 1142 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3880 160
Size: 872 2848 1606
Fragments arranged by size:
2848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2360 2651 1870
Size: 73 110 307 291 4545
Fragments arranged by size:
4545 307 291 110 73
BamHI G'GATC_C
Cuts at: 2656 3023 2656
Size: 367 4959
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2647 4102 618
Size: 325 217 627 860 1455 1842
Fragments arranged by size:
1842 1455 860 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2160 2645 1391
Size: 769 485 4072
Fragments arranged by size:
4072 769 485
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2018 2072 928
Size: 971 119 54 4182
Fragments arranged by size:
4182 971 119 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 1903
Size: 34 1580 34 3678
Fragments arranged by size:
3678 1580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3218 4428 3218
Size: 1210 4116
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5326
BfrBI ATG'CAT
Cuts at: 2486 2486
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3937 136
Size: 107 122 71 631 605 2265 1525
Fragments arranged by size:
2265 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5321 5321
Size: 5326
BlpI GC'TnA_GC
Cuts at: 2743 2743
Size: 5326
Bme1580I G_kGCm'C
Cuts at: 1790 3387 4543 1790
Size: 1597 1156 2573
Fragments arranged by size:
2573 1597 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3057 4015 442
Size: 416 53 83 399 24 1640 958 1753
Fragments arranged by size:
1753 1640 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3986 1239
Size: 2747 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3451 4316 4614 3451
Size: 865 298 4163
Fragments arranged by size:
4163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2444 3989 882
Size: 617 945 1545 2219
Fragments arranged by size:
2219 1545 945 617
BsaAI yAC'GTr
Cuts at: 493 1204 3117 493
Size: 711 1913 2702
Fragments arranged by size:
2702 1913 711
BsaBI GATnn'nnATC
Cuts at: 5320 5320
Size: 5326
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2343 3133 3515
Size: 53 83 186 936 564 246 790 382
Cuts at: 3515 4150 275
Size: 635 1451
Fragments arranged by size:
1451 936 790 635 564 382 246 186
83 53
BsaWI w'CCGG_w
Cuts at: 2301 2581 3759 4157 4431 4578 4989 2301
Size: 280 1178 398 274 147 411 2638
Fragments arranged by size:
2638 1178 411 398 280 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5101
Fragments arranged by size:
5101 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2611 1186
Size: 1153 272 3901
Fragments arranged by size:
3901 1153 272
BseYI C'CCAG_C
Cuts at: 4529 5025 4529
Size: 496 4830
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4753
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2776 3537 4565 1540
Size: 275 186 775 761 1028 2301
Fragments arranged by size:
2301 1028 775 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2331 3387 3472 4543 2160
Size: 171 1056 85 1071 2943
Fragments arranged by size:
2943 1071 1056 171 85
BsmI GAATG_Cn'
Cuts at: 2837 2930 2837
Size: 93 5233
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2331 2645 3387 3472 4543 1391
Size: 399 370 171 314 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 314 171 85
BspEI T'CCGG_A
Cuts at: 2301 4989 2301
Size: 2688 2638
BspHI T'CATG_A
Cuts at: 3215 4945 3215
Size: 1730 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2681 844
Size: 1837 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 1394
Size: 429 1390 3507
Fragments arranged by size:
3507 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2253 3821 3995 65
Size: 1183 1005 1568 174 1396
Fragments arranged by size:
1568 1396 1183 1005 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 3970 4157 1700
Size: 110 388 1772 187 2869
Fragments arranged by size:
2869 1772 388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 3386 4398 3386
Size: 1012 4314
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5326
BstBI TT'CG_AA
Cuts at: 4130 4130
Size: 5326
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5326
BstYI r'GATC_y
Cuts at: 1972 2656 3023 3408 3425 4877 4963 4975 5321
Size: 684 367 385 17 1452 86 12 346
Cuts at: 5321 1972
Size: 1977
Fragments arranged by size:
1977 1452 684 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3835
Fragments arranged by size:
3835 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4587
Fragments arranged by size:
4587 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3636 3656 2842
Size: 794 20 4512
Fragments arranged by size:
4512 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2140 2630 2140
Size: 490 4836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 809
Size: 507 3017 1802
Fragments arranged by size:
3017 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2773 3662 5291
Size: 54 1425 50 275 18 943 889 1629
Cuts at: 5291 8
Size: 43
Fragments arranged by size:
1629 1425 943 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2773 1537
Size: 275 961 4090
Fragments arranged by size:
4090 961 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2588 3256 1353
Size: 1235 668 3423
Fragments arranged by size:
3423 1235 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3913 4287 4433 387
Size: 1064 138 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2158 2158
Size: 5326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3389 3986 4773 5041 1239
Size: 393 1757 597 787 268 1524
Fragments arranged by size:
1757 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2236 2236
Size: 5326
EcoRI G'AATT_C
Cuts at: 2635 3013 2635
Size: 378 4948
EcoRV GAT'ATC
Cuts at: 2509 2509
Size: 5326
FspI TGC'GCA
Cuts at: 3832 3832
Size: 5326
HaeII r_GCGC'y
Cuts at: 1678 4473 1678
Size: 2795 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2559 2591 3967 3999 4041
Size: 32 135 32 987 32 1376 32 42
Cuts at: 4041 4073 1373
Size: 32 2626
Fragments arranged by size:
2626 1376 987 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KpnI G_GTAC'C
Cuts at: 2651 2651
Size: 5326
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4440 4624 1305
Size: 3135 184 2007
Fragments arranged by size:
3135 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3504
Fragments arranged by size:
3504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 3284 3643 3802 518
Size: 648 1308 810 359 159 2042
Fragments arranged by size:
2042 1308 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2742 3419 4567 4812 1606
Size: 1136 677 1148 245 2120
Fragments arranged by size:
2120 1148 1136 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3835
Fragments arranged by size:
3835 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2773 2773
Size: 5326
NsiI A_TGCA'T
Cuts at: 2488 2488
Size: 5326
NspI r_CATG'y
Cuts at: 1132 1229 2684 4229 1132
Size: 97 1455 1545 2229
Fragments arranged by size:
2229 1545 1455 97
PasI CC'CwG_GG
Cuts at: 1348 2551 1348
Size: 1203 4123
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5326
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 4908 4940 3395
Size: 32 1481 32 3781
Fragments arranged by size:
3781 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2072 1613
Size: 459 4867
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PstI C_TGCA'G
Cuts at: 830 2184 2341 2678 830
Size: 1354 157 337 3478
Fragments arranged by size:
3478 1354 337 157
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
SacI G_AGCT'C
Cuts at: 2160 2160
Size: 5326
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 3574 5286 3574
Size: 1712 3614
SfcI C'TryA_G
Cuts at: 826 1045 2180 2236 2337 2674 3809 4490 4681
Size: 219 1135 56 101 337 1135 681 191
Cuts at: 4681 826
Size: 1471
Fragments arranged by size:
1471 1135 1135 681 337 219 191 101
56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2653 1872
Size: 781 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3430 4331 4593 820
Size: 197 926 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3117 493
Size: 2624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2684 1132
Size: 1552 3774
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5326
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2765 513
Size: 552 939 396 365 3074
Fragments arranged by size:
3074 939 552 396 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3353 3370 3523 3708 4153 325
Size: 1255 1773 17 153 185 445 1498
Fragments arranged by size:
1773 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3572 5284 96
Size: 274 80 33 51 1443 1595 1712 138
Fragments arranged by size:
1712 1595 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2056 2088 2356 2388 5286 5318 2056
Size: 32 268 32 2898 32 2064
Fragments arranged by size:
2898 2064 268 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5326
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 2473
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5326
XmaI C'CCGG_G
Cuts at: 1870 2651 1870
Size: 781 4545
XmnI GAAnn'nnTTC
Cuts at: 2211 2288 3455 2211
Size: 77 1167 4082
Fragments arranged by size:
4082 1167 77
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2344 3134 276
Size: 53 83 186 936 810 790 2468
Fragments arranged by size:
2468 936 810 790 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI