Restriction map for
pFN21AB6926
(Circular) (Six-base) MAPSORT of: pFN21AB6926.seq Check: 5084 from: 1 to: 5389
pFN21AB6926 5389 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3199 278
Size: 53 83 186 936 1663 2468
Fragments arranged by size:
2468 1663 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2732 1937
Size: 795 4594
Acc65I G'GTAC_C
Cuts at: 2710 2710
Size: 5389
AclI AA'CG_TT
Cuts at: 1563 3516 3889 1563
Size: 1953 373 3063
Fragments arranged by size:
3063 1953 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2203 3452 4836 5104 1632
Size: 571 1249 1384 268 1917
Fragments arranged by size:
1917 1384 1249 571 268
AfeI AGC'GCT
Cuts at: 2666 2666
Size: 5389
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5192
AflIII A'CryG_T
Cuts at: 1203 2147 4288 5265 1203
Size: 944 2141 977 1327
Fragments arranged by size:
2141 1327 977 944
AgeI A'CCGG_T
Cuts at: 4220 4220
Size: 5389
AhdI GACnn_n'nnGTC
Cuts at: 4118 4118
Size: 5389
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 1184
Size: 948 4441
AlwNI CAG_nnn'CTG
Cuts at: 2080 2388 2676 3132 4704 2080
Size: 308 288 456 1572 2765
Fragments arranged by size:
2765 1572 456 308 288
ApaLI G'TGCA_C
Cuts at: 3446 4602 3446
Size: 1156 4233
ApoI r'AATT_y
Cuts at: 1493 2698 2924 3076 5185 1493
Size: 1205 226 152 2109 1697
Fragments arranged by size:
2109 1697 1205 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3943 160
Size: 872 2911 1606
Fragments arranged by size:
2911 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
AvaI C'yCGr_G
Cuts at: 1870 1943 2714 1870
Size: 73 771 4545
Fragments arranged by size:
4545 771 73
BamHI G'GATC_C
Cuts at: 2719 3086 2719
Size: 367 5022
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2710 4165 618
Size: 325 217 627 923 1455 1842
Fragments arranged by size:
1842 1455 923 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2625 2708 1391
Size: 1234 83 4072
Fragments arranged by size:
4072 1234 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2493 928
Size: 971 594 3824
Fragments arranged by size:
3824 971 594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3580 3614 1903
Size: 34 1643 34 3678
Fragments arranged by size:
3678 1643 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3281 4491 3281
Size: 1210 4179
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 4507
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 4000 136
Size: 107 122 71 631 605 901 1427 1525
Fragments arranged by size:
1525 1427 901 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2265 5384 2265
Size: 3119 2270
BlpI GC'TnA_GC
Cuts at: 2806 2806
Size: 5389
Bme1580I G_kGCm'C
Cuts at: 1790 2097 3450 4606 1790
Size: 307 1353 1156 2573
Fragments arranged by size:
2573 1353 1156 307
BmgBI CAC'GTC
Cuts at: 1318 2425 1318
Size: 1107 4282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3120 4078 442
Size: 416 53 83 399 24 1703 958 1753
Fragments arranged by size:
1753 1703 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2549 2653 4049 1239
Size: 1310 104 1396 2579
Fragments arranged by size:
2579 1396 1310 104
Bpu10I CC'TnA_GC
Cuts at: 2048 2328 2048
Size: 280 5109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3514 4379 4677 3514
Size: 865 298 4226
Fragments arranged by size:
4226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2489 2525 4052 882
Size: 617 990 36 1527 2219
Fragments arranged by size:
2219 1527 990 617 36
BsaAI yAC'GTr
Cuts at: 493 1204 3180 493
Size: 711 1976 2702
Fragments arranged by size:
2702 1976 711
BsaBI GATnn'nnATC
Cuts at: 5383 5383
Size: 5389
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 3196 3578 4213
Size: 53 83 186 936 603 1060 382 635
Cuts at: 4213 275
Size: 1451
Fragments arranged by size:
1451 1060 936 635 603 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2186 3822 4220 4494 4641 5052 2186
Size: 1636 398 274 147 411 2523
Fragments arranged by size:
2523 1636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5164
Fragments arranged by size:
5164 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5389
BseYI C'CCAG_C
Cuts at: 2279 2624 4592 5088 2279
Size: 345 1968 496 2580
Fragments arranged by size:
2580 1968 496 345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4816
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2839 3600 4628 1540
Size: 275 186 838 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3450 3535 4606 3450
Size: 85 1071 4233
Fragments arranged by size:
4233 1071 85
BsmI GAATG_Cn'
Cuts at: 2900 2993 2900
Size: 93 5296
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2625 2708 3450 3535 4606 1391
Size: 399 307 528 83 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 528 399 307 85 83
BspEI T'CCGG_A
Cuts at: 2186 5052 2186
Size: 2866 2523
BspHI T'CATG_A
Cuts at: 2404 3278 5008 2404
Size: 874 1730 2785
Fragments arranged by size:
2785 1730 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2744 844
Size: 1900 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3276 1394
Size: 429 1453 3507
Fragments arranged by size:
3507 1453 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3884 4058 65
Size: 1183 2636 174 1396
Fragments arranged by size:
2636 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 4033 4220 1700
Size: 110 228 1995 187 2869
Fragments arranged by size:
2869 1995 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5389
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5389
BssSI C'ACGA_G
Cuts at: 3449 4461 3449
Size: 1012 4377
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 794
Size: 1286 4103
BstBI TT'CG_AA
Cuts at: 4193 4193
Size: 5389
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5389
BstYI r'GATC_y
Cuts at: 1972 2265 2634 2719 3086 3471 3488 4940 5026
Size: 293 369 85 367 385 17 1452 86
Cuts at: 5026 5038 5384 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 369 367 346 293 86
85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2098 2098
Size: 5389
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4837
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2563 487
Size: 683 56 1337 3313
Fragments arranged by size:
3313 1337 683 56
BtsI GCAGTG_nn'
Cuts at: 2905 3699 3719 2905
Size: 794 20 4575
Fragments arranged by size:
4575 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5354
DraI TTT'AAA
Cuts at: 2693 2693
Size: 5389
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2135 4396 809
Size: 507 819 2261 1802
Fragments arranged by size:
2261 1802 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2502 2574 2836
Size: 54 1425 50 275 18 672 72 262
Cuts at: 2836 3725 5354 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 672 275 262 72 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2836 1537
Size: 275 1024 4090
Fragments arranged by size:
4090 1024 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3319 1353
Size: 1966 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2468 2630 3976 4350 4496 387
Size: 1064 138 879 162 1346 374 146 1280
Fragments arranged by size:
1346 1280 1064 879 374 162 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2203 2549 2653 3452 4049 4836 5104
Size: 393 571 346 104 799 597 787 268
Cuts at: 5104 1239
Size: 1524
Fragments arranged by size:
1524 799 787 597 571 393 346 268
104
EcoNI CCTnn'n_nnAGG
Cuts at: 2503 2503
Size: 5389
EcoO109I rG'GnC_Cy
Cuts at: 2175 2385 2175
Size: 210 5179
EcoRI G'AATT_C
Cuts at: 2698 3076 2698
Size: 378 5011
FspI TGC'GCA
Cuts at: 3895 3895
Size: 5389
HaeII r_GCGC'y
Cuts at: 1678 2541 2668 4536 1678
Size: 863 127 1868 2531
Fragments arranged by size:
2531 1868 863 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4030 4062 4104 4136 1373
Size: 32 135 32 2458 32 42 32 2626
Fragments arranged by size:
2626 2458 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2733 2979 669
Size: 988 281 795 246 3079
Fragments arranged by size:
3079 988 795 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5389
HpaI GTT'AAC
Cuts at: 2979 2979
Size: 5389
KpnI G_GTAC'C
Cuts at: 2714 2714
Size: 5389
MfeI C'AATT_G
Cuts at: 2988 2988
Size: 5389
MluI A'CGCG_T
Cuts at: 5265 5265
Size: 5389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4503 4687 1305
Size: 3198 184 2007
Fragments arranged by size:
3198 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2504 2576 10
Size: 54 1425 343 672 72 2823
Fragments arranged by size:
2823 1425 672 343 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2409 2555 3347 3706 3865 518
Size: 648 1243 146 792 359 159 2042
Fragments arranged by size:
2042 1243 792 648 359 159 146
MspA1I CmG'CkG
Cuts at: 1606 2279 2673 2805 3482 4630 4875 1606
Size: 673 394 132 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 673 394 245 132
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4837
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5389
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5389
NotI GC'GGCC_GC
Cuts at: 2836 2836
Size: 5389
NspI r_CATG'y
Cuts at: 1132 1229 2151 2747 4292 1132
Size: 97 922 596 1545 2229
Fragments arranged by size:
2229 1545 922 596 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5389
PciI A'CATG_T
Cuts at: 2147 4288 2147
Size: 2141 3248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5389
PfoI T'CCnGG_A
Cuts at: 2526 2526
Size: 5389
PmeI GTTT'AAAC
Cuts at: 2693 2693
Size: 5389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 4971 5003 3458
Size: 32 1481 32 3844
Fragments arranged by size:
3844 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5389
PsiI TTA'TAA
Cuts at: 2959 2959
Size: 5389
PstI C_TGCA'G
Cuts at: 830 2265 2741 830
Size: 1435 476 3478
Fragments arranged by size:
3478 1435 476
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5389
PvuII CAG'CTG
Cuts at: 2673 2673
Size: 5389
SalI G'TCGA_C
Cuts at: 1936 2731 1936
Size: 795 4594
SbfI CC_TGCA'GG
Cuts at: 2741 2741
Size: 5389
ScaI AGT'ACT
Cuts at: 3637 5349 3637
Size: 1712 3677
SfcI C'TryA_G
Cuts at: 826 1045 2261 2737 3872 4553 4744 826
Size: 219 1216 476 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 681 476 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5389
SmaI CCC'GGG
Cuts at: 1872 2716 1872
Size: 844 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3493 4394 4656 820
Size: 197 926 1550 901 262 1553
Fragments arranged by size:
1553 1550 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3180 493
Size: 2687 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5389
SphI G_CATG'C
Cuts at: 1132 2747 1132
Size: 1615 3774
SspI AAT'ATT
Cuts at: 5 52 3313 5
Size: 47 3261 2081
Fragments arranged by size:
3261 2081 47
StuI AGG'CCT
Cuts at: 1517 2333 1517
Size: 816 4573
StyI C'CwwG_G
Cuts at: 513 1065 2499 2583 2828 513
Size: 552 1434 84 245 3074
Fragments arranged by size:
3074 1434 552 245 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 3416 3433 3586 3771 4216 325
Size: 1255 741 1095 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1095 741 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3635 5347 96
Size: 274 80 33 51 1443 1658 1712 138
Fragments arranged by size:
1712 1658 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5349 5381 5349
Size: 32 5357
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5389
XbaI T'CTAG_A
Cuts at: 2725 2725
Size: 5389
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5389
XmaI C'CCGG_G
Cuts at: 1870 2714 1870
Size: 844 4545
XmnI GAAnn'nnTTC
Cuts at: 3518 3518
Size: 5389
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3197 276
Size: 53 83 186 936 1663 2468
Fragments arranged by size:
2468 1663 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI