Restriction map for
pFN21AB6882
(Circular) (Six-base) MAPSORT of: pFN21AB6882.seq Check: 277 from: 1 to: 5308
pFN21AB6882 5308 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3118 278
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2379 2651 1937
Size: 442 272 4594
Fragments arranged by size:
4594 442 272
Acc65I G'GTAC_C
Cuts at: 2629 2629
Size: 5308
AclI AA'CG_TT
Cuts at: 1563 3435 3808 1563
Size: 1872 373 3063
Fragments arranged by size:
3063 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 4755 5023 1632
Size: 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5111
AflIII A'CryG_T
Cuts at: 1203 2262 2394 4207 5184 1203
Size: 1059 132 1813 977 1327
Fragments arranged by size:
1813 1327 1059 977 132
AgeI A'CCGG_T
Cuts at: 4139 4139
Size: 5308
AhdI GACnn_n'nnGTC
Cuts at: 4037 4037
Size: 5308
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5308
AlwNI CAG_nnn'CTG
Cuts at: 3051 4623 3051
Size: 1572 3736
ApaLI G'TGCA_C
Cuts at: 3365 4521 3365
Size: 1156 4152
ApoI r'AATT_y
Cuts at: 1493 2617 2843 2995 5104 1493
Size: 1124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3862 160
Size: 872 2830 1606
Fragments arranged by size:
2830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 2472 2633 1870
Size: 73 210 319 161 4545
Fragments arranged by size:
4545 319 210 161 73
BamHI G'GATC_C
Cuts at: 2638 3005 2638
Size: 367 4941
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 2438 2629 4084 618
Size: 325 217 627 495 156 191 1455 1842
Fragments arranged by size:
1842 1455 627 495 325 217 191 156
BanII G_rGCy'C
Cuts at: 1391 2627 1391
Size: 1236 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2288 2322 2381 2415 3499 3533 1903
Size: 34 351 34 59 34 1084 34 3678
Fragments arranged by size:
3678 1084 351 59 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3200 4410 3200
Size: 1210 4098
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3919 136
Size: 107 122 71 631 605 2247 1525
Fragments arranged by size:
2247 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5303 5303
Size: 5308
BlpI GC'TnA_GC
Cuts at: 2725 2725
Size: 5308
Bme1580I G_kGCm'C
Cuts at: 1790 2285 3369 4525 1790
Size: 495 1084 1156 2573
Fragments arranged by size:
2573 1156 1084 495
BmgBI CAC'GTC
Cuts at: 1318 2263 1318
Size: 945 4363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2266 2338 3039
Size: 416 53 83 399 24 849 72 701
Cuts at: 3039 3997 442
Size: 958 1753
Fragments arranged by size:
1753 958 849 701 416 399 83 72
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 3968 1239
Size: 1094 1635 2579
Fragments arranged by size:
2579 1635 1094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3433 4298 4596 3433
Size: 865 298 4145
Fragments arranged by size:
4145 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2198 3971 882
Size: 617 699 1773 2219
Fragments arranged by size:
2219 1773 699 617
BsaAI yAC'GTr
Cuts at: 493 1204 2397 3099 493
Size: 711 1193 702 2702
Fragments arranged by size:
2702 1193 711 702
BsaBI GATnn'nnATC
Cuts at: 5302 5302
Size: 5308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3115 3497 4132 275
Size: 53 83 186 936 1582 382 635 1451
Fragments arranged by size:
1582 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3741 4139 4413 4560 4971 3741
Size: 398 274 147 411 4078
Fragments arranged by size:
4078 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 2519 2549 1178
Size: 30 165 30 1038 30 48 30 3937
Fragments arranged by size:
3937 1038 165 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 1186
Size: 1357 3951
BseYI C'CCAG_C
Cuts at: 2041 4511 5007 2041
Size: 2470 496 2342
Fragments arranged by size:
2470 2342 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 1163
Size: 573 690 4045
Fragments arranged by size:
4045 690 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2053 2758 3519 4547 1540
Size: 275 186 52 705 761 1028 2301
Fragments arranged by size:
2301 1028 761 705 275 186 52
BsiHKAI G_wGCw'C
Cuts at: 3369 3454 4525 3369
Size: 85 1071 4152
Fragments arranged by size:
4152 1071 85
BsmI GAATG_Cn'
Cuts at: 2819 2912 2819
Size: 93 5215
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2627 3369 3454 4525 1391
Size: 399 495 342 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 399 342 85
BspEI T'CCGG_A
Cuts at: 4971 4971
Size: 5308
BspHI T'CATG_A
Cuts at: 3197 4927 3197
Size: 1730 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 844
Size: 1819 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 1394
Size: 429 1372 3507
Fragments arranged by size:
3507 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 3977 65
Size: 1183 2555 174 1396
Fragments arranged by size:
2555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2207 3952 4139 1700
Size: 110 397 1745 187 2869
Fragments arranged by size:
2869 1745 397 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5308
BssSI C'ACGA_G
Cuts at: 3368 4380 3368
Size: 1012 4296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5308
BstBI TT'CG_AA
Cuts at: 4112 4112
Size: 5308
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5308
BstYI r'GATC_y
Cuts at: 1972 2638 3005 3390 3407 4859 4945 4957 5303
Size: 666 367 385 17 1452 86 12 346
Cuts at: 5303 1972
Size: 1977
Fragments arranged by size:
1977 1452 666 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2136 2196 513
Size: 552 1071 60 3625
Fragments arranged by size:
3625 1071 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2595 487
Size: 683 56 1369 3200
Fragments arranged by size:
3200 1369 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 3618 3638 2824
Size: 794 20 4494
Fragments arranged by size:
4494 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5273
DraI TTT'AAA
Cuts at: 2612 2612
Size: 5308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2180 4315 809
Size: 507 864 2135 1802
Fragments arranged by size:
2135 1802 864 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2139 2209
Size: 54 1425 50 275 18 220 89 70
Cuts at: 2209 2541 2755 3644 5273 8
Size: 332 214 889 1629 43
Fragments arranged by size:
1629 1425 889 332 275 220 214 89
70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2050 2755 1537
Size: 275 238 705 4090
Fragments arranged by size:
4090 705 275 238
EarI CTCTTCn'nnn_
Cuts at: 1353 3238 1353
Size: 1885 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3895 4269 4415 387
Size: 1064 138 2306 374 146 1280
Fragments arranged by size:
2306 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 3371 3968 4755 5023 1239
Size: 393 701 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 787 701 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2124 2149 2158 2302 2476 2124
Size: 25 9 144 174 4956
Fragments arranged by size:
4956 174 144 25 9
EcoRI G'AATT_C
Cuts at: 2617 2995 2617
Size: 378 4930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2131 2116
Size: 15 5293
FspI TGC'GCA
Cuts at: 2269 3814 2269
Size: 1545 3763
HaeII r_GCGC'y
Cuts at: 1678 2024 2042 2223 4455 1678
Size: 346 18 181 2232 2531
Fragments arranged by size:
2531 2232 346 181 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 2573 2605 3949
Size: 32 135 32 869 32 100 32 1344
Cuts at: 3949 3981 4023 4055 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1344 869 135 100 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2380 2652 2898 669
Size: 988 281 442 272 246 3079
Fragments arranged by size:
3079 988 442 281 272 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5308
HpaI GTT'AAC
Cuts at: 2898 2898
Size: 5308
KpnI G_GTAC'C
Cuts at: 2633 2633
Size: 5308
MfeI C'AATT_G
Cuts at: 2907 2907
Size: 5308
MluI A'CGCG_T
Cuts at: 5184 5184
Size: 5308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4422 4606 1305
Size: 3117 184 2007
Fragments arranged by size:
3117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 2543 10
Size: 54 1425 343 309 402 2775
Fragments arranged by size:
2775 1425 402 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3266 3625 3784 518
Size: 648 2100 359 159 2042
Fragments arranged by size:
2100 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2521 2724 3401 4549 4794 1606
Size: 443 472 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 472 443 245 203
NcoI C'CATG_G
Cuts at: 513 1065 2136 513
Size: 552 1071 3685
Fragments arranged by size:
3685 1071 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5308
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5308
NotI GC'GGCC_GC
Cuts at: 2050 2755 2050
Size: 705 4603
NspI r_CATG'y
Cuts at: 1132 1229 2666 4211 1132
Size: 97 1437 1545 2229
Fragments arranged by size:
2229 1545 1437 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5308
PciI A'CATG_T
Cuts at: 4207 4207
Size: 5308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4406
PmeI GTTT'AAAC
Cuts at: 2612 2612
Size: 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 4890 4922 3377
Size: 32 1481 32 3763
Fragments arranged by size:
3763 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2124 2149 2302 2476 2124
Size: 25 153 174 4956
Fragments arranged by size:
4956 174 153 25
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5308
PsiI TTA'TAA
Cuts at: 2878 2878
Size: 5308
PstI C_TGCA'G
Cuts at: 830 2172 2660 830
Size: 1342 488 3478
Fragments arranged by size:
3478 1342 488
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5308
SalI G'TCGA_C
Cuts at: 1936 2378 2650 1936
Size: 442 272 4594
Fragments arranged by size:
4594 442 272
SanDI GG'GwC_CC
Cuts at: 2476 2476
Size: 5308
SbfI CC_TGCA'GG
Cuts at: 2660 2660
Size: 5308
ScaI AGT'ACT
Cuts at: 2327 2411 3556 5268 2327
Size: 84 1145 1712 2367
Fragments arranged by size:
2367 1712 1145 84
SexAI A'CCwGG_T
Cuts at: 2126 2126
Size: 5308
SfcI C'TryA_G
Cuts at: 826 1045 2168 2656 3791 4472 4663 826
Size: 219 1123 488 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 681 488 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5308
SmaI CCC'GGG
Cuts at: 1872 2474 2635 1872
Size: 602 161 4545
Fragments arranged by size:
4545 602 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3412 4313 4575 820
Size: 197 926 1469 901 262 1553
Fragments arranged by size:
1553 1469 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3099 493
Size: 2606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5308
SphI G_CATG'C
Cuts at: 1132 2666 1132
Size: 1534 3774
SspI AAT'ATT
Cuts at: 5 52 3232 5
Size: 47 3180 2081
Fragments arranged by size:
3180 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5308
StyI C'CwwG_G
Cuts at: 513 1065 2136 2229 2747 513
Size: 552 1071 93 518 3074
Fragments arranged by size:
3074 1071 552 518 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2002 3335 3352 3505 3690 4135 325
Size: 1255 422 1333 17 153 185 445 1498
Fragments arranged by size:
1498 1333 1255 445 422 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 2409 2550
Size: 274 80 33 51 1443 348 84 141
Cuts at: 2550 3554 5266 96
Size: 1004 1712 138
Fragments arranged by size:
1712 1443 1004 348 274 141 138 84
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2519 2551 5268 5300 2519
Size: 32 2717 32 2527
Fragments arranged by size:
2717 2527 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5308
XbaI T'CTAG_A
Cuts at: 2644 2644
Size: 5308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5308
XmaI C'CCGG_G
Cuts at: 1870 2472 2633 1870
Size: 602 161 4545
Fragments arranged by size:
4545 602 161
XmnI GAAnn'nnTTC
Cuts at: 3437 3437
Size: 5308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3116 276
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI
SfiI SfoI SrfI SwaI XcmI