Restriction map for
pFN21AB6878
(Circular) (Six-base) MAPSORT of: pFN21AB6878.seq Check: 5134 from: 1 to: 5305
pFN21AB6878 5305 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2134 2134
Size: 5305
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3115 278
Size: 53 83 186 936 1579 2468
Fragments arranged by size:
2468 1579 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2648 1937
Size: 711 4594
Acc65I G'GTAC_C
Cuts at: 2626 2626
Size: 5305
AclI AA'CG_TT
Cuts at: 1563 3432 3805 1563
Size: 1869 373 3063
Fragments arranged by size:
3063 1869 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 3368 4752 5020 1632
Size: 458 1278 1384 268 1917
Fragments arranged by size:
1917 1384 1278 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5108
AflIII A'CryG_T
Cuts at: 1203 4204 5181 1203
Size: 3001 977 1327
Fragments arranged by size:
3001 1327 977
AgeI A'CCGG_T
Cuts at: 4136 4136
Size: 5305
AhdI GACnn_n'nnGTC
Cuts at: 2083 4034 2083
Size: 1951 3354
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5305
AlwNI CAG_nnn'CTG
Cuts at: 2260 2317 2489 2561 3048 4620 2260
Size: 57 172 72 487 1572 2945
Fragments arranged by size:
2945 1572 487 172 72 57
ApaLI G'TGCA_C
Cuts at: 3362 4518 3362
Size: 1156 4149
ApoI r'AATT_y
Cuts at: 1493 2614 2840 2992 5101 1493
Size: 1121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3859 160
Size: 872 2827 1606
Fragments arranged by size:
2827 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
AvaI C'yCGr_G
Cuts at: 1870 1943 2630 1870
Size: 73 687 4545
Fragments arranged by size:
4545 687 73
BamHI G'GATC_C
Cuts at: 2635 3002 2635
Size: 367 4938
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2626 4081 618
Size: 325 217 627 839 1455 1842
Fragments arranged by size:
1842 1455 839 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 2532 2624 1391
Size: 632 509 92 4072
Fragments arranged by size:
4072 632 509 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3496 3530 1903
Size: 34 1559 34 3678
Fragments arranged by size:
3678 1559 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3197 4407 3197
Size: 1210 4095
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5305
BfrBI ATG'CAT
Cuts at: 2188 2188
Size: 5305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3916 136
Size: 107 122 71 631 605 2244 1525
Fragments arranged by size:
2244 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2370 5300 2370
Size: 2930 2375
BlpI GC'TnA_GC
Cuts at: 2722 2722
Size: 5305
Bme1580I G_kGCm'C
Cuts at: 1790 3366 4522 1790
Size: 1576 1156 2573
Fragments arranged by size:
2573 1576 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2526 3036 3994
Size: 416 53 83 399 24 1109 510 958
Cuts at: 3994 442
Size: 1753
Fragments arranged by size:
1753 1109 958 510 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5305
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 5305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2414 3965 1239
Size: 1175 1551 2579
Fragments arranged by size:
2579 1551 1175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2404 3430 4295 4593 2234
Size: 170 1026 865 298 2946
Fragments arranged by size:
2946 1026 865 298 170
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3968 882
Size: 617 2469 2219
Fragments arranged by size:
2469 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3096 493
Size: 711 1892 2702
Fragments arranged by size:
2702 1892 711
BsaBI GATnn'nnATC
Cuts at: 5299 5299
Size: 5305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2519 3112 3494 4129
Size: 53 83 186 936 986 593 382 635
Cuts at: 4129 275
Size: 1451
Fragments arranged by size:
1451 986 936 635 593 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2343 3738 4136 4410 4557 4968 2343
Size: 1395 398 274 147 411 2680
Fragments arranged by size:
2680 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 2357 2387 2417
Size: 30 165 30 861 30 63 30 30
Cuts at: 2417 1178
Size: 4066
Fragments arranged by size:
4066 861 165 63 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2411 2414 1186
Size: 1162 63 3 4077
Fragments arranged by size:
4077 1162 63 3
BseYI C'CCAG_C
Cuts at: 2010 4508 5004 2010
Size: 2498 496 2311
Fragments arranged by size:
2498 2311 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4732
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 2755 3516 4544 1540
Size: 275 186 343 411 761 1028 2301
Fragments arranged by size:
2301 1028 761 411 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 3366 3451 4522 2601
Size: 765 85 1071 3384
Fragments arranged by size:
3384 1071 765 85
BsmI GAATG_Cn'
Cuts at: 2816 2909 2816
Size: 93 5212
BsmBI CGTCTCn'nnnn_
Cuts at: 2033 2132 2358 2033
Size: 99 226 4980
Fragments arranged by size:
4980 226 99
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2532 2601 2624 3366 3451 4522
Size: 399 233 509 69 23 742 85 1071
Cuts at: 4522 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 509 399 233 85 69
23
BspEI T'CCGG_A
Cuts at: 4968 4968
Size: 5305
BspHI T'CATG_A
Cuts at: 3194 4924 3194
Size: 1730 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 2451 2660 844
Size: 1290 317 209 3489
Fragments arranged by size:
3489 1290 317 209
BsrBI CCG'CTC
Cuts at: 1394 1823 3192 1394
Size: 429 1369 3507
Fragments arranged by size:
3507 1369 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3800 3974 65
Size: 1183 2552 174 1396
Fragments arranged by size:
2552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2216 3949 4136 1700
Size: 110 406 1733 187 2869
Fragments arranged by size:
2869 1733 406 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5305
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5305
BssSI C'ACGA_G
Cuts at: 3365 4377 3365
Size: 1012 4293
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2561 794
Size: 1767 3538
BstBI TT'CG_AA
Cuts at: 4109 4109
Size: 5305
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5305
BstXI CCAn_nnnn'nTGG
Cuts at: 2579 2579
Size: 5305
BstYI r'GATC_y
Cuts at: 1972 2370 2635 3002 3387 3404 4856 4942 4954
Size: 398 265 367 385 17 1452 86 12
Cuts at: 4954 5300 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 398 385 367 346 265 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3814
Fragments arranged by size:
3814 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4566
Fragments arranged by size:
4566 683 56
BtsI GCAGTG_nn'
Cuts at: 2324 2821 3615 3635 2324
Size: 497 794 20 3994
Fragments arranged by size:
3994 794 497 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5270
DraI TTT'AAA
Cuts at: 2609 2609
Size: 5305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2146 4312 809
Size: 507 830 2166 1802
Fragments arranged by size:
2166 1802 830 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2752 3641 5270
Size: 54 1425 50 275 18 922 889 1629
Cuts at: 5270 8
Size: 43
Fragments arranged by size:
1629 1425 922 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2752 1537
Size: 275 940 4090
Fragments arranged by size:
4090 940 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3235 1353
Size: 1882 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3892 4266 4412 387
Size: 1064 138 2303 374 146 1280
Fragments arranged by size:
2303 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2414 3368 3965 4752 5020 1239
Size: 393 458 324 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 597 458 393 324 268
EcoRI G'AATT_C
Cuts at: 2614 2992 2614
Size: 378 4927
FspI TGC'GCA
Cuts at: 2321 3811 2321
Size: 1490 3815
HaeII r_GCGC'y
Cuts at: 1678 4452 1678
Size: 2774 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2264 2296 2355
Size: 32 135 32 497 32 163 32 59
Cuts at: 2355 2357 2387 2389 2471 2486 2503 2518 3946
Size: 2 30 2 82 15 17 15 1428
Cuts at: 3946 3978 4020 4052 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1428 497 163 135 82 59 42
32 32 32 32 32 32 30 17
15 15 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 2895 669
Size: 988 281 711 246 3079
Fragments arranged by size:
3079 988 711 281 246
HindIII A'AGCT_T
Cuts at: 748 2457 748
Size: 1709 3596
HpaI GTT'AAC
Cuts at: 2895 2895
Size: 5305
KpnI G_GTAC'C
Cuts at: 2630 2630
Size: 5305
MfeI C'AATT_G
Cuts at: 2904 2904
Size: 5305
MluI A'CGCG_T
Cuts at: 5181 5181
Size: 5305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 4419 4603 1305
Size: 963 2151 184 2007
Fragments arranged by size:
2151 2007 963 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3483
Fragments arranged by size:
3483 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2571 2577 3263 3622 3781 518
Size: 648 1405 6 686 359 159 2042
Fragments arranged by size:
2042 1405 686 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2317 2721 3398 4546 4791 1606
Size: 711 404 677 1148 245 2120
Fragments arranged by size:
2120 1148 711 677 404 245
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 5305
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3814
Fragments arranged by size:
3814 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5305
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 5305
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5305
NotI GC'GGCC_GC
Cuts at: 2752 2752
Size: 5305
NsiI A_TGCA'T
Cuts at: 2190 2190
Size: 5305
NspI r_CATG'y
Cuts at: 1132 1229 2192 2663 4208 1132
Size: 97 963 471 1545 2229
Fragments arranged by size:
2229 1545 963 471 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5305
PciI A'CATG_T
Cuts at: 4204 4204
Size: 5305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5305
PmeI GTTT'AAAC
Cuts at: 2609 2609
Size: 5305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 4887 4919 3374
Size: 32 1481 32 3760
Fragments arranged by size:
3760 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5305
PsiI TTA'TAA
Cuts at: 2875 2875
Size: 5305
PstI C_TGCA'G
Cuts at: 830 2052 2331 2657 830
Size: 1222 279 326 3478
Fragments arranged by size:
3478 1222 326 279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5305
PvuII CAG'CTG
Cuts at: 2317 2317
Size: 5305
SalI G'TCGA_C
Cuts at: 1936 2647 1936
Size: 711 4594
SbfI CC_TGCA'GG
Cuts at: 2657 2657
Size: 5305
ScaI AGT'ACT
Cuts at: 3553 5265 3553
Size: 1712 3593
SfcI C'TryA_G
Cuts at: 826 1045 2048 2327 2546 2653 3788 4469 4660
Size: 219 1003 279 219 107 1135 681 191
Cuts at: 4660 826
Size: 1471
Fragments arranged by size:
1471 1135 1003 681 279 219 219 191
107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5305
SmaI CCC'GGG
Cuts at: 1872 2632 1872
Size: 760 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 2419 3409 4310 4572 820
Size: 197 926 306 170 990 901 262 1553
Fragments arranged by size:
1553 990 926 901 306 262 197 170
SnaBI TAC'GTA
Cuts at: 493 3096 493
Size: 2603 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5305
SphI G_CATG'C
Cuts at: 1132 2663 1132
Size: 1531 3774
SspI AAT'ATT
Cuts at: 5 52 3229 5
Size: 47 3177 2081
Fragments arranged by size:
3177 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5305
StyI C'CwwG_G
Cuts at: 513 1065 2004 2744 513
Size: 552 939 740 3074
Fragments arranged by size:
3074 939 740 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3332 3349 3502 3687 4132 325
Size: 1255 1752 17 153 185 445 1498
Fragments arranged by size:
1752 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3551 5263 96
Size: 274 80 33 51 1443 1574 1712 138
Fragments arranged by size:
1712 1574 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5265 5297 5265
Size: 32 5273
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5305
XbaI T'CTAG_A
Cuts at: 2641 2641
Size: 5305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2573 2573
Size: 5305
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5305
XmaI C'CCGG_G
Cuts at: 1870 2630 1870
Size: 760 4545
XmnI GAAnn'nnTTC
Cuts at: 3434 3434
Size: 5305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3113 276
Size: 53 83 186 936 1579 2468
Fragments arranged by size:
2468 1579 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI