Restriction map for
pFN21AB6862
(Circular) (Six-base) MAPSORT of: pFN21AB6862.seq Check: 1510 from: 1 to: 5290
pFN21AB6862 5290 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3100 278
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2633 1937
Size: 696 4594
Acc65I G'GTAC_C
Cuts at: 2611 2611
Size: 5290
AclI AA'CG_TT
Cuts at: 1563 2388 3417 3790 1563
Size: 825 1029 373 3063
Fragments arranged by size:
3063 1029 825 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2476 3353 4737 5005 1632
Size: 467 377 877 1384 268 1917
Fragments arranged by size:
1917 1384 877 467 377 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5093
AflIII A'CryG_T
Cuts at: 1203 4189 5166 1203
Size: 2986 977 1327
Fragments arranged by size:
2986 1327 977
AgeI A'CCGG_T
Cuts at: 4121 4121
Size: 5290
AhdI GACnn_n'nnGTC
Cuts at: 4019 4019
Size: 5290
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2379 2546 1184
Size: 1195 167 3928
Fragments arranged by size:
3928 1195 167
AlwNI CAG_nnn'CTG
Cuts at: 3033 4605 3033
Size: 1572 3718
ApaLI G'TGCA_C
Cuts at: 3347 4503 3347
Size: 1156 4134
ApoI r'AATT_y
Cuts at: 1493 2599 2825 2977 5086 1493
Size: 1106 226 152 2109 1697
Fragments arranged by size:
2109 1697 1106 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3844 160
Size: 872 2812 1606
Fragments arranged by size:
2812 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
AvaI C'yCGr_G
Cuts at: 1870 1943 2615 1870
Size: 73 672 4545
Fragments arranged by size:
4545 672 73
BamHI G'GATC_C
Cuts at: 2301 2620 2987 2301
Size: 319 367 4604
Fragments arranged by size:
4604 367 319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2198 2611 4066 618
Size: 325 217 627 305 106 413 1455 1842
Fragments arranged by size:
1842 1455 627 413 325 305 217 106
BanII G_rGCy'C
Cuts at: 1391 2064 2208 2282 2463 2609 1391
Size: 673 144 74 181 146 4072
Fragments arranged by size:
4072 673 181 146 144 74
BbeI G_GCGC'C
Cuts at: 2202 2202
Size: 5290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3481 3515 1903
Size: 34 1544 34 3678
Fragments arranged by size:
3678 1544 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3182 4392 3182
Size: 1210 4080
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3901 136
Size: 107 122 71 631 605 2229 1525
Fragments arranged by size:
2229 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5285 5285
Size: 5290
BlpI GC'TnA_GC
Cuts at: 2707 2707
Size: 5290
Bme1580I G_kGCm'C
Cuts at: 1790 3351 4507 1790
Size: 1561 1156 2573
Fragments arranged by size:
2573 1561 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5290
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2551 3021 3979
Size: 416 53 83 399 24 1134 470 958
Cuts at: 3979 442
Size: 1753
Fragments arranged by size:
1753 1134 958 470 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2207 2304 2522 2600 3950 1239
Size: 821 147 97 218 78 1350 2579
Fragments arranged by size:
2579 1350 821 218 147 97 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 3415 4280 4578 2048
Size: 1367 865 298 2760
Fragments arranged by size:
2760 1367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2183 3953 882
Size: 617 684 1770 2219
Fragments arranged by size:
2219 1770 684 617
BsaAI yAC'GTr
Cuts at: 493 1204 3081 493
Size: 711 1877 2702
Fragments arranged by size:
2702 1877 711
BsaBI GATnn'nnATC
Cuts at: 5284 5284
Size: 5290
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 2148 2199 3097
Size: 53 83 186 936 489 126 51 898
Cuts at: 3097 3479 4114 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 898 635 489 382 186 126
83 53 51
BsaWI w'CCGG_w
Cuts at: 3723 4121 4395 4542 4953 3723
Size: 398 274 147 411 4060
Fragments arranged by size:
4060 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 1178
Size: 30 165 30 1140 30 3895
Fragments arranged by size:
3895 1140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2304 2525 1186
Size: 886 232 221 3951
Fragments arranged by size:
3951 886 232 221
BseYI C'CCAG_C
Cuts at: 2297 4493 4989 2297
Size: 2196 496 2598
Fragments arranged by size:
2598 2196 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2324 1163
Size: 573 588 4129
Fragments arranged by size:
4129 588 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2740 3501 4529 1540
Size: 275 186 739 761 1028 2301
Fragments arranged by size:
2301 1028 761 739 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2208 3351 3436 4507 2064
Size: 144 1143 85 1071 2847
Fragments arranged by size:
2847 1143 1071 144 85
BsmI GAATG_Cn'
Cuts at: 2801 2894 2801
Size: 93 5197
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2208 2282 2463 2609 3351 3436
Size: 399 274 144 74 181 146 742 85
Cuts at: 3436 4507 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 274 181 146 144
85 74
BspEI T'CCGG_A
Cuts at: 4953 4953
Size: 5290
BspHI T'CATG_A
Cuts at: 3179 4909 3179
Size: 1730 3560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2528 2645 844
Size: 1684 117 3489
Fragments arranged by size:
3489 1684 117
BsrBI CCG'CTC
Cuts at: 1394 1823 2584 3177 1394
Size: 429 761 593 3507
Fragments arranged by size:
3507 761 593 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3785 3959 65
Size: 1183 2537 174 1396
Fragments arranged by size:
2537 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3934 4121 1700
Size: 110 2124 187 2869
Fragments arranged by size:
2869 2124 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5290
BssHII G'CGCG_C
Cuts at: 1925 2011 2013 2015 2130 1925
Size: 86 2 2 115 5085
Fragments arranged by size:
5085 115 86 2 2
BssSI C'ACGA_G
Cuts at: 3350 4362 3350
Size: 1012 4278
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5290
BstBI TT'CG_AA
Cuts at: 4094 4094
Size: 5290
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5290
BstXI CCAn_nnnn'nTGG
Cuts at: 2282 2282
Size: 5290
BstYI r'GATC_y
Cuts at: 1972 2301 2620 2987 3372 3389 4841 4927 4939
Size: 329 319 367 385 17 1452 86 12
Cuts at: 4939 5285 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 329 319 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2546 2546
Size: 5290
BtgI C'CryG_G
Cuts at: 513 1065 2004 2123 2142 513
Size: 552 939 119 19 3661
Fragments arranged by size:
3661 939 552 119 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4551
Fragments arranged by size:
4551 683 56
BtsI GCAGTG_nn'
Cuts at: 2454 2806 3600 3620 2454
Size: 352 794 20 4124
Fragments arranged by size:
4124 794 352 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5255
DraI TTT'AAA
Cuts at: 2594 2594
Size: 5290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2171 4297 809
Size: 507 855 2126 1802
Fragments arranged by size:
2126 1802 855 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2247 2265 2737
Size: 54 1425 50 275 18 417 18 472
Cuts at: 2737 3626 5255 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 472 417 275 54 50
43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2737 1537
Size: 275 925 4090
Fragments arranged by size:
4090 925 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 3220 1353
Size: 1142 725 3423
Fragments arranged by size:
3423 1142 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3877 4251 4397 387
Size: 1064 138 2288 374 146 1280
Fragments arranged by size:
2288 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 2206 2062
Size: 144 5146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2099 2207 2304 2476 2522 2600
Size: 393 428 39 108 97 172 46 78
Cuts at: 2600 3353 3950 4737 5005 1239
Size: 753 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 428 393 268 172
108 97 78 46 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 2570
Size: 5290
EcoO109I rG'GnC_Cy
Cuts at: 2308 2422 2542 2575 2308
Size: 114 120 33 5023
Fragments arranged by size:
5023 120 114 33
EcoRI G'AATT_C
Cuts at: 2599 2977 2599
Size: 378 4912
FspI TGC'GCA
Cuts at: 3796 3796
Size: 5290
HaeII r_GCGC'y
Cuts at: 1678 2202 4437 1678
Size: 524 2235 2531
Fragments arranged by size:
2531 2235 524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2543 2575 3931 3963 4005
Size: 32 135 32 971 32 1356 32 42
Cuts at: 4005 4037 1373
Size: 32 2626
Fragments arranged by size:
2626 1356 971 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2634 2880 669
Size: 988 281 696 246 3079
Fragments arranged by size:
3079 988 696 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5290
HpaI GTT'AAC
Cuts at: 2880 2880
Size: 5290
KasI G'GCGC_C
Cuts at: 2198 2198
Size: 5290
KpnI G_GTAC'C
Cuts at: 2615 2615
Size: 5290
MfeI C'AATT_G
Cuts at: 2889 2889
Size: 5290
MluI A'CGCG_T
Cuts at: 5166 5166
Size: 5290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4404 4588 1305
Size: 3099 184 2007
Fragments arranged by size:
3099 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3468
Fragments arranged by size:
3468 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3248 3607 3766 518
Size: 648 2082 359 159 2042
Fragments arranged by size:
2082 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2125 2257 2297 2706 3383 4531 4776
Size: 492 27 132 40 409 677 1148 245
Cuts at: 4776 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 492 409 245 132 40
27
NarI GG'CG_CC
Cuts at: 2199 2199
Size: 5290
NcoI C'CATG_G
Cuts at: 513 1065 2004 2142 513
Size: 552 939 138 3661
Fragments arranged by size:
3661 939 552 138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5290
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5290
NotI GC'GGCC_GC
Cuts at: 2737 2737
Size: 5290
NruI TCG'CGA
Cuts at: 2375 2375
Size: 5290
NspI r_CATG'y
Cuts at: 1132 1229 2648 4193 1132
Size: 97 1419 1545 2229
Fragments arranged by size:
2229 1545 1419 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5290
PciI A'CATG_T
Cuts at: 4189 4189
Size: 5290
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5290
PfoI T'CCnGG_A
Cuts at: 2028 2028
Size: 5290
PmeI GTTT'AAAC
Cuts at: 2594 2594
Size: 5290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 4872 4904 3359
Size: 32 1481 32 3745
Fragments arranged by size:
3745 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2422 2575 2422
Size: 153 5137
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5290
PsiI TTA'TAA
Cuts at: 2860 2860
Size: 5290
PstI C_TGCA'G
Cuts at: 830 2241 2262 2642 830
Size: 1411 21 380 3478
Fragments arranged by size:
3478 1411 380 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5290
PvuII CAG'CTG
Cuts at: 2257 2297 2257
Size: 40 5250
SacI G_AGCT'C
Cuts at: 2064 2208 2064
Size: 144 5146
SacII CC_GC'GG
Cuts at: 2126 2126
Size: 5290
SalI G'TCGA_C
Cuts at: 1936 2632 1936
Size: 696 4594
SapI GCTCTTCn'nnn_
Cuts at: 2495 2495
Size: 5290
SbfI CC_TGCA'GG
Cuts at: 2241 2642 2241
Size: 401 4889
ScaI AGT'ACT
Cuts at: 3538 5250 3538
Size: 1712 3578
SexAI A'CCwGG_T
Cuts at: 2565 2565
Size: 5290
SfcI C'TryA_G
Cuts at: 826 1045 2237 2258 2638 3773 4454 4645 826
Size: 219 1192 21 380 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 681 380 219 191 21
SfoI GGC'GCC
Cuts at: 2200 2200
Size: 5290
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5290
SmaI CCC'GGG
Cuts at: 1872 2617 1872
Size: 745 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 3394 4295 4557 820
Size: 197 926 120 1331 901 262 1553
Fragments arranged by size:
1553 1331 926 901 262 197 120
SnaBI TAC'GTA
Cuts at: 493 3081 493
Size: 2588 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5290
SphI G_CATG'C
Cuts at: 1132 2648 1132
Size: 1516 3774
SspI AAT'ATT
Cuts at: 5 52 3214 5
Size: 47 3162 2081
Fragments arranged by size:
3162 2081 47
StuI AGG'CCT
Cuts at: 1517 2337 1517
Size: 820 4470
StyI C'CwwG_G
Cuts at: 513 1065 2004 2142 2151 2729 513
Size: 552 939 138 9 578 3074
Fragments arranged by size:
3074 939 578 552 138 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3317 3334 3487 3672 4117 325
Size: 1255 1737 17 153 185 445 1498
Fragments arranged by size:
1737 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3536 5248 96
Size: 274 80 33 51 1443 1559 1712 138
Fragments arranged by size:
1712 1559 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5250 5282 5250
Size: 32 5258
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5290
XbaI T'CTAG_A
Cuts at: 2626 2626
Size: 5290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2511 2149
Size: 362 4928
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5290
XmaI C'CCGG_G
Cuts at: 1870 2615 1870
Size: 745 4545
XmnI GAAnn'nnTTC
Cuts at: 2489 3419 2489
Size: 930 4360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3098 276
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV
FalI FseI FspAI NaeI NgoMIV NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SanDI SfiI SrfI SwaI