Restriction map for
pFN21AB6836
(Circular) (Six-base) MAPSORT of: pFN21AB6836.seq Check: 7638 from: 1 to: 5230
pFN21AB6836 5230 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3040 278
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2573 1937
Size: 636 4594
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 2283 3293 4677 4945 1632
Size: 593 58 1010 1384 268 1917
Fragments arranged by size:
1917 1384 1010 593 268 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 4129 5106 1203
Size: 2926 977 1327
Fragments arranged by size:
2926 1327 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 3959 3959
Size: 5230
AleI CACnn'nnGTG
Cuts at: 1166 2039 1166
Size: 873 4357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5230
AlwNI CAG_nnn'CTG
Cuts at: 2973 4545 2973
Size: 1572 3658
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2539 2765 2917 5026 1493
Size: 1046 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 1870
Size: 73 612 4545
Fragments arranged by size:
4545 612 73
BamHI G'GATC_C
Cuts at: 2560 2927 2560
Size: 367 4863
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 4006 618
Size: 325 217 627 764 1455 1842
Fragments arranged by size:
1842 1455 764 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2549 1391
Size: 621 537 4072
Fragments arranged by size:
4072 621 537
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3421 3455 1903
Size: 34 1484 34 3678
Fragments arranged by size:
3678 1484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4332 3122
Size: 1210 4020
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5230
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3841 136
Size: 107 122 71 631 605 2169 1525
Fragments arranged by size:
2169 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5225 5225
Size: 5230
BlpI GC'TnA_GC
Cuts at: 2647 2647
Size: 5230
Bme1580I G_kGCm'C
Cuts at: 1790 3291 4447 1790
Size: 1501 1156 2573
Fragments arranged by size:
2573 1501 1156
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 4507
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2961 3919 442
Size: 416 53 83 399 24 1544 958 1753
Fragments arranged by size:
1753 1544 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2414 3890 1239
Size: 1094 81 1476 2579
Fragments arranged by size:
2579 1476 1094 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 3355 4220 4518 2504
Size: 851 865 298 3216
Fragments arranged by size:
3216 865 851 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3893 882
Size: 617 2394 2219
Fragments arranged by size:
2394 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2289 3037 3419 4054
Size: 53 83 186 936 756 748 382 635
Cuts at: 4054 275
Size: 1451
Fragments arranged by size:
1451 936 756 748 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3663 4061 4335 4482 4893 3663
Size: 398 274 147 411 4000
Fragments arranged by size:
4000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5005
Fragments arranged by size:
5005 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 1186
Size: 1003 4227
BseYI C'CCAG_C
Cuts at: 2467 2483 2488 4433 4929 2467
Size: 16 5 1945 496 2768
Fragments arranged by size:
2768 1945 496 16 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4657
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2122 2230 2680 3441 4469 1540
Size: 275 186 121 108 450 761 1028 2301
Fragments arranged by size:
2301 1028 761 450 275 186 121 108
BsiHKAI G_wGCw'C
Cuts at: 2012 3291 3376 4447 2012
Size: 1279 85 1071 2795
Fragments arranged by size:
2795 1279 1071 85
BsmI GAATG_Cn'
Cuts at: 2741 2834 2741
Size: 93 5137
BsmBI CGTCTCn'nnnn_
Cuts at: 2204 2204
Size: 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2549 3291 3376 4447 1391
Size: 399 222 537 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 537 399 222 85
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 3119 4849 3119
Size: 1730 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2585 844
Size: 1241 500 3489
Fragments arranged by size:
3489 1241 500
BsrBI CCG'CTC
Cuts at: 1394 1823 3117 1394
Size: 429 1294 3507
Fragments arranged by size:
3507 1294 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3725 3899 65
Size: 1183 2477 174 1396
Fragments arranged by size:
2477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3874 4061 1700
Size: 110 2064 187 2869
Fragments arranged by size:
2869 2064 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5230
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5230
BssSI C'ACGA_G
Cuts at: 3290 4302 3290
Size: 1012 4218
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5230
BstBI TT'CG_AA
Cuts at: 2505 4034 2505
Size: 1529 3701
BstEII G'GTnAC_C
Cuts at: 1184 2441 1184
Size: 1257 3973
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2311 2041
Size: 270 4960
BstYI r'GATC_y
Cuts at: 1972 2341 2560 2927 3312 3329 4781 4867 4879
Size: 369 219 367 385 17 1452 86 12
Cuts at: 4879 5225 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 369 367 346 219 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 3439
Fragments arranged by size:
3439 1239 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2139 487
Size: 683 56 913 3578
Fragments arranged by size:
3578 913 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3540 3560 2746
Size: 794 20 4416
Fragments arranged by size:
4416 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5195
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2227 2677
Size: 54 1425 50 275 18 339 58 450
Cuts at: 2677 3566 5195 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 450 339 275 58 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2227 2677 1537
Size: 275 415 450 4090
Fragments arranged by size:
4090 450 415 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 2507 3160 1353
Size: 890 264 653 3423
Fragments arranged by size:
3423 890 653 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3817 4191 4337 387
Size: 1064 138 2228 374 146 1280
Fragments arranged by size:
2228 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2283 2333 2414 3293 3890 4677
Size: 393 593 58 50 81 879 597 787
Cuts at: 4677 4945 1239
Size: 268 1524
Fragments arranged by size:
1524 879 787 597 593 393 268 81
58 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 2411
Size: 5230
EcoO109I rG'GnC_Cy
Cuts at: 2332 2332
Size: 5230
EcoRI G'AATT_C
Cuts at: 2539 2917 2539
Size: 378 4852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2441 2441
Size: 5230
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 4377 1678
Size: 2699 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3871 3903 3945 3977 1373
Size: 32 135 32 2299 32 42 32 2626
Fragments arranged by size:
2626 2299 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2056 2574 2820 669
Size: 988 281 118 518 246 3079
Fragments arranged by size:
3079 988 518 281 246 118
HindIII A'AGCT_T
Cuts at: 748 2423 748
Size: 1675 3555
HpaI GTT'AAC
Cuts at: 2820 2820
Size: 5230
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4344 4528 1305
Size: 3039 184 2007
Fragments arranged by size:
3039 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3408
Fragments arranged by size:
3408 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2382 3188 3547 3706 518
Size: 648 873 343 806 359 159 2042
Fragments arranged by size:
2042 873 806 648 359 343 159
MspA1I CmG'CkG
Cuts at: 1606 2261 2646 3323 4471 4716 1606
Size: 655 385 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 655 385 245
NcoI C'CATG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 3439
Fragments arranged by size:
3439 1239 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2227 2677 2227
Size: 450 4780
NspI r_CATG'y
Cuts at: 1132 1229 2588 4133 1132
Size: 97 1359 1545 2229
Fragments arranged by size:
2229 1545 1359 97
PasI CC'CwG_GG
Cuts at: 1348 2353 1348
Size: 1005 4225
PciI A'CATG_T
Cuts at: 4129 4129
Size: 5230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2467 1108
Size: 1359 3871
PfoI T'CCnGG_A
Cuts at: 2241 2391 2241
Size: 150 5080
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5230
PsiI TTA'TAA
Cuts at: 2800 2800
Size: 5230
PstI C_TGCA'G
Cuts at: 830 2082 2582 830
Size: 1252 500 3478
Fragments arranged by size:
3478 1252 500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
PvuII CAG'CTG
Cuts at: 2261 2261
Size: 5230
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5230
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SbfI CC_TGCA'GG
Cuts at: 2082 2582 2082
Size: 500 4730
ScaI AGT'ACT
Cuts at: 3478 5190 3478
Size: 1712 3518
SfcI C'TryA_G
Cuts at: 826 1045 2078 2578 3713 4394 4585 826
Size: 219 1033 500 1135 681 191 1471
Fragments arranged by size:
1471 1135 1033 681 500 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5230
SmaI CCC'GGG
Cuts at: 1872 2557 1872
Size: 685 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 3334 4235 4497 820
Size: 197 926 576 815 901 262 1553
Fragments arranged by size:
1553 926 901 815 576 262 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5230
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 3154 5
Size: 47 3102 2081
Fragments arranged by size:
3102 2081 47
StuI AGG'CCT
Cuts at: 1517 2084 1517
Size: 567 4663
StyI C'CwwG_G
Cuts at: 513 1065 2304 2669 513
Size: 552 1239 365 3074
Fragments arranged by size:
3074 1239 552 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2429 2459 3257 3274 3427 3612 4057
Size: 1255 849 30 798 17 153 185 445
Cuts at: 4057 325
Size: 1498
Fragments arranged by size:
1498 1255 849 798 445 185 153 30
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3476 5188 96
Size: 274 80 33 51 1443 1499 1712 138
Fragments arranged by size:
1712 1499 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5190 5222 5190
Size: 32 5198
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5230
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 2468
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2555 1870
Size: 685 4545
XmnI GAAnn'nnTTC
Cuts at: 3359 3359
Size: 5230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3038 276
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI