Restriction map for
pFN21AB6786
(Circular) (Six-base) MAPSORT of: pFN21AB6786.seq Check: 8056 from: 1 to: 5167
pFN21AB6786 5167 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2977 278
Size: 53 83 186 936 1441 2468
Fragments arranged by size:
2468 1441 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2510 1937
Size: 573 4594
Acc65I G'GTAC_C
Cuts at: 2488 2488
Size: 5167
AclI AA'CG_TT
Cuts at: 1563 3294 3667 1563
Size: 1731 373 3063
Fragments arranged by size:
3063 1731 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 3230 4614 4882 1632
Size: 719 879 1384 268 1917
Fragments arranged by size:
1917 1384 879 719 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4970
AflIII A'CryG_T
Cuts at: 1203 2245 4066 5043 1203
Size: 1042 1821 977 1327
Fragments arranged by size:
1821 1327 1042 977
AgeI A'CCGG_T
Cuts at: 3998 3998
Size: 5167
AhdI GACnn_n'nnGTC
Cuts at: 3896 3896
Size: 5167
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5167
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 2069
Size: 32 5135
AlwNI CAG_nnn'CTG
Cuts at: 2910 4482 2910
Size: 1572 3595
ApaLI G'TGCA_C
Cuts at: 3224 4380 3224
Size: 1156 4011
ApoI r'AATT_y
Cuts at: 1493 2476 2702 2854 4963 1493
Size: 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3721 160
Size: 872 2689 1606
Fragments arranged by size:
2689 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5167
AvaI C'yCGr_G
Cuts at: 1870 1943 2492 1870
Size: 73 549 4545
Fragments arranged by size:
4545 549 73
BamHI G'GATC_C
Cuts at: 2497 2864 2497
Size: 367 4800
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2488 3943 618
Size: 325 217 627 701 1455 1842
Fragments arranged by size:
1842 1455 701 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2486 1391
Size: 1095 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2291 928
Size: 971 392 3804
Fragments arranged by size:
3804 971 392
BbvCI CC'TCA_GC
Cuts at: 2292 2292
Size: 5167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3358 3392 1903
Size: 34 1421 34 3678
Fragments arranged by size:
3678 1421 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3059 4269 3059
Size: 1210 3957
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2441 3778 136
Size: 107 122 71 631 605 769 1337 1525
Fragments arranged by size:
1525 1337 769 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5162 5162
Size: 5167
BlpI GC'TnA_GC
Cuts at: 2106 2584 2106
Size: 478 4689
Bme1580I G_kGCm'C
Cuts at: 1790 3228 4384 1790
Size: 1438 1156 2573
Fragments arranged by size:
2573 1438 1156
BmgBI CAC'GTC
Cuts at: 1318 2097 1318
Size: 779 4388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2277 2898 3856
Size: 416 53 83 399 24 860 621 958
Cuts at: 3856 442
Size: 1753
Fragments arranged by size:
1753 958 860 621 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3827 1239
Size: 2588 2579
Bpu10I CC'TnA_GC
Cuts at: 2292 2448 2460 2292
Size: 156 12 4999
Fragments arranged by size:
4999 156 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 3292 4157 4455 2042
Size: 1250 865 298 2754
Fragments arranged by size:
2754 1250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3830 882
Size: 617 2331 2219
Fragments arranged by size:
2331 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2248 2958 493
Size: 711 1044 710 2702
Fragments arranged by size:
2702 1044 711 710
BsaBI GATnn'nnATC
Cuts at: 5161 5161
Size: 5167
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2974 3356 3991 275
Size: 53 83 186 936 1441 382 635 1451
Fragments arranged by size:
1451 1441 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3600 3998 4272 4419 4830 3600
Size: 398 274 147 411 3937
Fragments arranged by size:
3937 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 1178
Size: 30 165 30 672 30 4240
Fragments arranged by size:
4240 672 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5167
BseYI C'CCAG_C
Cuts at: 4370 4866 4370
Size: 496 4671
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4594
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2617 3378 4406 1540
Size: 275 186 616 761 1028 2301
Fragments arranged by size:
2301 1028 761 616 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 3228 3313 4384 2253
Size: 975 85 1071 3036
Fragments arranged by size:
3036 1071 975 85
BsmI GAATG_Cn'
Cuts at: 2678 2771 2678
Size: 93 5074
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2486 3228 3313 4384 1391
Size: 399 463 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 463 399 233 85
BspEI T'CCGG_A
Cuts at: 4830 4830
Size: 5167
BspHI T'CATG_A
Cuts at: 3056 4786 3056
Size: 1730 3437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 844
Size: 1678 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3054 1394
Size: 429 1231 3507
Fragments arranged by size:
3507 1231 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2100 3662 3836 65
Size: 1183 852 1562 174 1396
Fragments arranged by size:
1562 1396 1183 852 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3811 3998 1700
Size: 110 2001 187 2869
Fragments arranged by size:
2869 2001 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5167
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5167
BssSI C'ACGA_G
Cuts at: 3227 4239 3227
Size: 1012 4155
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5167
BstBI TT'CG_AA
Cuts at: 3971 3971
Size: 5167
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5167
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 2344 2134
Size: 210 4957
BstYI r'GATC_y
Cuts at: 1972 2497 2864 3249 3266 4718 4804 4816 5162
Size: 525 367 385 17 1452 86 12 346
Cuts at: 5162 1972
Size: 1977
Fragments arranged by size:
1977 1452 525 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3676
Fragments arranged by size:
3676 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4428
Fragments arranged by size:
4428 683 56
BtsI GCAGTG_nn'
Cuts at: 2683 3477 3497 2683
Size: 794 20 4353
Fragments arranged by size:
4353 794 20
ClaI AT'CG_AT
Cuts at: 1554 2256 1554
Size: 702 4465
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2083 2118 529
Size: 35 1519 35 3578
Fragments arranged by size:
3578 1519 35 35
DraI TTT'AAA
Cuts at: 2471 2471
Size: 5167
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4174 809
Size: 507 2858 1802
Fragments arranged by size:
2858 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2614 3503 5132
Size: 54 1425 50 275 18 784 889 1629
Cuts at: 5132 8
Size: 43
Fragments arranged by size:
1629 1425 889 784 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2614 1537
Size: 275 802 4090
Fragments arranged by size:
4090 802 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2370 3097 1353
Size: 1017 727 3423
Fragments arranged by size:
3423 1017 727
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3754 4128 4274 387
Size: 1064 138 2165 374 146 1280
Fragments arranged by size:
2165 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 3230 3827 4614 4882 1239
Size: 393 719 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 719 597 393 268
EcoRI G'AATT_C
Cuts at: 2476 2854 2476
Size: 378 4789
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 5167
FspI TGC'GCA
Cuts at: 3673 3673
Size: 5167
HaeII r_GCGC'y
Cuts at: 1678 4314 1678
Size: 2636 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 3808 3840 3882
Size: 32 135 32 503 32 1701 32 42
Cuts at: 3882 3914 1373
Size: 32 2626
Fragments arranged by size:
2626 1701 503 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2511 2757 669
Size: 988 281 573 246 3079
Fragments arranged by size:
3079 988 573 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5167
HpaI GTT'AAC
Cuts at: 2757 2757
Size: 5167
KpnI G_GTAC'C
Cuts at: 2492 2492
Size: 5167
MfeI C'AATT_G
Cuts at: 2766 2766
Size: 5167
MluI A'CGCG_T
Cuts at: 5043 5043
Size: 5167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2380 4281 4465 1305
Size: 1075 1901 184 2007
Fragments arranged by size:
2007 1901 1075 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3345
Fragments arranged by size:
3345 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2402 3125 3484 3643 518
Size: 648 1092 144 723 359 159 2042
Fragments arranged by size:
2042 1092 723 648 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 2068 2291 2583 3260 4408 4653 1606
Size: 462 223 292 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 462 292 245 223
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3676
Fragments arranged by size:
3676 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5167
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5167
NotI GC'GGCC_GC
Cuts at: 2614 2614
Size: 5167
NspI r_CATG'y
Cuts at: 1132 1229 2525 4070 1132
Size: 97 1296 1545 2229
Fragments arranged by size:
2229 1545 1296 97
PasI CC'CwG_GG
Cuts at: 1348 2222 2437 1348
Size: 874 215 4078
Fragments arranged by size:
4078 874 215
PciI A'CATG_T
Cuts at: 4066 4066
Size: 5167
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 1108
Size: 972 4195
PmeI GTTT'AAAC
Cuts at: 2471 2471
Size: 5167
PmlI CAC'GTG
Cuts at: 2248 2248
Size: 5167
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2235 2267 3236 3268 4749 4781 2235
Size: 32 969 32 1481 32 2621
Fragments arranged by size:
2621 1481 969 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5167
PsiI TTA'TAA
Cuts at: 2737 2737
Size: 5167
PstI C_TGCA'G
Cuts at: 830 2519 830
Size: 1689 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5167
PvuII CAG'CTG
Cuts at: 2068 2291 2068
Size: 223 4944
SalI G'TCGA_C
Cuts at: 1936 2509 1936
Size: 573 4594
SapI GCTCTTCn'nnn_
Cuts at: 2370 2370
Size: 5167
SbfI CC_TGCA'GG
Cuts at: 2519 2519
Size: 5167
ScaI AGT'ACT
Cuts at: 3415 5127 3415
Size: 1712 3455
SfcI C'TryA_G
Cuts at: 826 1045 2515 3650 4331 4522 826
Size: 219 1470 1135 681 191 1471
Fragments arranged by size:
1471 1470 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5167
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5167
SmaI CCC'GGG
Cuts at: 1872 2494 1872
Size: 622 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 3271 4172 4434 820
Size: 197 926 114 1214 901 262 1553
Fragments arranged by size:
1553 1214 926 901 262 197 114
SnaBI TAC'GTA
Cuts at: 493 2958 493
Size: 2465 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5167
SphI G_CATG'C
Cuts at: 1132 2525 1132
Size: 1393 3774
SspI AAT'ATT
Cuts at: 5 52 3091 5
Size: 47 3039 2081
Fragments arranged by size:
3039 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5167
StyI C'CwwG_G
Cuts at: 513 1065 2004 2142 2301 2606 513
Size: 552 939 138 159 305 3074
Fragments arranged by size:
3074 939 552 305 159 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3194 3211 3364 3549 3994 325
Size: 1255 1614 17 153 185 445 1498
Fragments arranged by size:
1614 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3413 5125 96
Size: 274 80 33 51 1443 1436 1712 138
Fragments arranged by size:
1712 1443 1436 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5127 5159 5127
Size: 32 5135
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5167
XbaI T'CTAG_A
Cuts at: 2503 2503
Size: 5167
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5167
XmaI C'CCGG_G
Cuts at: 1870 2492 1870
Size: 622 4545
XmnI GAAnn'nnTTC
Cuts at: 3296 3296
Size: 5167
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2975 276
Size: 53 83 186 936 1441 2468
Fragments arranged by size:
2468 1441 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI