Restriction map for
pFN21AB6781
(Circular) (Six-base) MAPSORT of: pFN21AB6781.seq Check: 4002 from: 1 to: 5065
pFN21AB6781 5065 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2120 2196 2120
Size: 76 4989
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2875 278
Size: 53 83 186 936 1339 2468
Fragments arranged by size:
2468 1339 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2408 1937
Size: 471 4594
Acc65I G'GTAC_C
Cuts at: 2386 2386
Size: 5065
AclI AA'CG_TT
Cuts at: 1563 3192 3565 1563
Size: 1629 373 3063
Fragments arranged by size:
3063 1629 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 3128 4512 4780 1632
Size: 509 987 1384 268 1917
Fragments arranged by size:
1917 1384 987 509 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4868
AflIII A'CryG_T
Cuts at: 1203 3964 4941 1203
Size: 2761 977 1327
Fragments arranged by size:
2761 1327 977
AgeI A'CCGG_T
Cuts at: 3896 3896
Size: 5065
AhdI GACnn_n'nnGTC
Cuts at: 3794 3794
Size: 5065
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5065
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 2090
Size: 32 5033
AlwNI CAG_nnn'CTG
Cuts at: 2808 4380 2808
Size: 1572 3493
ApaLI G'TGCA_C
Cuts at: 3122 4278 3122
Size: 1156 3909
ApoI r'AATT_y
Cuts at: 1493 2374 2600 2752 4861 1493
Size: 881 226 152 2109 1697
Fragments arranged by size:
2109 1697 881 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3619 160
Size: 872 2587 1606
Fragments arranged by size:
2587 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5065
AvaI C'yCGr_G
Cuts at: 1870 1943 2390 1870
Size: 73 447 4545
Fragments arranged by size:
4545 447 73
BamHI G'GATC_C
Cuts at: 2272 2395 2762 2272
Size: 123 367 4575
Fragments arranged by size:
4575 367 123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 3841 618
Size: 325 217 627 599 1455 1842
Fragments arranged by size:
1842 1455 627 599 325 217
BanII G_rGCy'C
Cuts at: 1391 2384 1391
Size: 993 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4094
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2211 2245 3256 3290 1903
Size: 34 274 34 1011 34 3678
Fragments arranged by size:
3678 1011 274 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2156 2957 4167 2156
Size: 801 1210 3054
Fragments arranged by size:
3054 1210 801
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5065
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3676 136
Size: 107 122 71 631 605 2004 1525
Fragments arranged by size:
2004 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5060 5060
Size: 5065
BlpI GC'TnA_GC
Cuts at: 2482 2482
Size: 5065
Bme1580I G_kGCm'C
Cuts at: 1790 3126 4282 1790
Size: 1336 1156 2573
Fragments arranged by size:
2573 1336 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5065
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 2796 3754
Size: 416 53 83 399 24 843 536 958
Cuts at: 3754 442
Size: 1753
Fragments arranged by size:
1753 958 843 536 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3725 1239
Size: 2486 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3190 4055 4353 3190
Size: 865 298 3902
Fragments arranged by size:
3902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 3728 882
Size: 617 518 1711 2219
Fragments arranged by size:
2219 1711 617 518
BsaAI yAC'GTr
Cuts at: 493 1204 2856 493
Size: 711 1652 2702
Fragments arranged by size:
2702 1652 711
BsaBI GATnn'nnATC
Cuts at: 5059 5059
Size: 5065
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2872 3254 3889 275
Size: 53 83 186 936 1339 382 635 1451
Fragments arranged by size:
1451 1339 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3498 3896 4170 4317 4728 3498
Size: 398 274 147 411 3835
Fragments arranged by size:
3835 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2334 2364 1178
Size: 30 165 30 931 30 3879
Fragments arranged by size:
3879 931 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 1186
Size: 1156 3909
BseYI C'CCAG_C
Cuts at: 2346 4268 4764 2346
Size: 1922 496 2647
Fragments arranged by size:
2647 1922 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2215 1163
Size: 573 479 4013
Fragments arranged by size:
4013 573 479
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2515 3276 4304 1540
Size: 275 186 514 761 1028 2301
Fragments arranged by size:
2301 1028 761 514 275 186
BsiHKAI G_wGCw'C
Cuts at: 3126 3211 4282 3126
Size: 85 1071 3909
Fragments arranged by size:
3909 1071 85
BsmI GAATG_Cn'
Cuts at: 2576 2669 2576
Size: 93 4972
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2384 3126 3211 4282 1391
Size: 399 594 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 399 85
BspEI T'CCGG_A
Cuts at: 4728 4728
Size: 5065
BspHI T'CATG_A
Cuts at: 2954 4684 2954
Size: 1730 3335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2120 2196 2420 844
Size: 1276 76 224 3489
Fragments arranged by size:
3489 1276 224 76
BsrBI CCG'CTC
Cuts at: 1394 1823 2952 1394
Size: 429 1129 3507
Fragments arranged by size:
3507 1129 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3560 3734 65
Size: 1183 2312 174 1396
Fragments arranged by size:
2312 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3709 3896 1700
Size: 110 1899 187 2869
Fragments arranged by size:
2869 1899 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5065
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5065
BssSI C'ACGA_G
Cuts at: 3125 4137 3125
Size: 1012 4053
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5065
BstBI TT'CG_AA
Cuts at: 3869 3869
Size: 5065
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5065
BstYI r'GATC_y
Cuts at: 1972 2272 2395 2762 3147 3164 4616 4702 4714
Size: 300 123 367 385 17 1452 86 12
Cuts at: 4714 5060 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 300 123 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2310 513
Size: 552 1245 3268
Fragments arranged by size:
3268 1245 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4326
Fragments arranged by size:
4326 683 56
BtsI GCAGTG_nn'
Cuts at: 2581 3375 3395 2581
Size: 794 20 4251
Fragments arranged by size:
4251 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5065
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5030
DraI TTT'AAA
Cuts at: 2369 2369
Size: 5065
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2252 4072 809
Size: 507 936 1820 1802
Fragments arranged by size:
1820 1802 936 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2512 3401 5030
Size: 54 1425 50 275 18 682 889 1629
Cuts at: 5030 8
Size: 43
Fragments arranged by size:
1629 1425 889 682 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2512 1537
Size: 275 700 4090
Fragments arranged by size:
4090 700 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2995 1353
Size: 1642 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3652 4026 4172 387
Size: 1064 138 2063 374 146 1280
Fragments arranged by size:
2063 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 3128 3725 4512 4780 1239
Size: 393 509 987 597 787 268 1524
Fragments arranged by size:
1524 987 787 597 509 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2173 2173
Size: 5065
EcoRI G'AATT_C
Cuts at: 2374 2752 2374
Size: 378 4687
FspI TGC'GCA
Cuts at: 3571 3571
Size: 5065
HaeII r_GCGC'y
Cuts at: 1678 4212 1678
Size: 2534 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3706 3738 3780 3812 1373
Size: 32 135 32 2134 32 42 32 2626
Fragments arranged by size:
2626 2134 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2342 2409 2655 669
Size: 988 281 404 67 246 3079
Fragments arranged by size:
3079 988 404 281 246 67
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5065
HpaI GTT'AAC
Cuts at: 2655 2655
Size: 5065
KpnI G_GTAC'C
Cuts at: 2390 2390
Size: 5065
MfeI C'AATT_G
Cuts at: 2664 2664
Size: 5065
MluI A'CGCG_T
Cuts at: 4941 4941
Size: 5065
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4179 4363 1305
Size: 2874 184 2007
Fragments arranged by size:
2874 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3243
Fragments arranged by size:
3243 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3023 3382 3541 518
Size: 648 1857 359 159 2042
Fragments arranged by size:
2042 1857 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2481 3158 4306 4551 1606
Size: 875 677 1148 245 2120
Fragments arranged by size:
2120 1148 875 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4513
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5065
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5065
NotI GC'GGCC_GC
Cuts at: 2512 2512
Size: 5065
NspI r_CATG'y
Cuts at: 1132 1229 2423 3968 1132
Size: 97 1194 1545 2229
Fragments arranged by size:
2229 1545 1194 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5065
PciI A'CATG_T
Cuts at: 3964 3964
Size: 5065
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5065
PmeI GTTT'AAAC
Cuts at: 2369 2369
Size: 5065
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 4647 4679 3134
Size: 32 1481 32 3520
Fragments arranged by size:
3520 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5065
PsiI TTA'TAA
Cuts at: 2635 2635
Size: 5065
PstI C_TGCA'G
Cuts at: 830 2417 830
Size: 1587 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5065
SalI G'TCGA_C
Cuts at: 1936 2407 1936
Size: 471 4594
SbfI CC_TGCA'GG
Cuts at: 2417 2417
Size: 5065
ScaI AGT'ACT
Cuts at: 3313 5025 3313
Size: 1712 3353
SfcI C'TryA_G
Cuts at: 826 1045 2413 3548 4229 4420 826
Size: 219 1368 1135 681 191 1471
Fragments arranged by size:
1471 1368 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5065
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5065
SmaI CCC'GGG
Cuts at: 1872 2392 1872
Size: 520 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3169 4070 4332 820
Size: 197 926 1226 901 262 1553
Fragments arranged by size:
1553 1226 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2856 493
Size: 2363 2702
SpeI A'CTAG_T
Cuts at: 152 2152 152
Size: 2000 3065
SphI G_CATG'C
Cuts at: 1132 2423 1132
Size: 1291 3774
SspI AAT'ATT
Cuts at: 5 52 2989 5
Size: 47 2937 2081
Fragments arranged by size:
2937 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5065
StyI C'CwwG_G
Cuts at: 513 1065 2144 2504 513
Size: 552 1079 360 3074
Fragments arranged by size:
3074 1079 552 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2083 3092 3109 3262 3447 3892 325
Size: 1255 503 1009 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1009 503 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 3311 5023
Size: 274 80 33 51 1443 285 1049 1712
Cuts at: 5023 96
Size: 138
Fragments arranged by size:
1712 1443 1049 285 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5025 5057 5025
Size: 32 5033
Tth111I GACn'n_nGTC
Cuts at: 1316 2252 1316
Size: 936 4129
XbaI T'CTAG_A
Cuts at: 2401 2401
Size: 5065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5065
XmaI C'CCGG_G
Cuts at: 1870 2390 1870
Size: 520 4545
XmnI GAAnn'nnTTC
Cuts at: 3194 3194
Size: 5065
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2873 276
Size: 53 83 186 936 1339 2468
Fragments arranged by size:
2468 1339 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI