Restriction map for
pFN21AB6696
(Circular) (Six-base) MAPSORT of: pFN21AB6696.seq Check: 6279 from: 1 to: 6091
pFN21AB6696 6091 bp
With 182 enzymes: SgfI *
November 19, 2009 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2766 2912 3220 4154 5538 5806 1632
Size: 1134 146 308 934 1384 268 1917
Fragments arranged by size:
1917 1384 1134 934 308 268 146
AfeI AGC'GCT
Cuts at: 2203 2203
Size: 6091
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2646 4990 5967 1203
Size: 1443 2344 977 1327
Fragments arranged by size:
2344 1443 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 2379 4820 2379
Size: 2441 3650
AleI CACnn'nnGTG
Cuts at: 1166 2166 1166
Size: 1000 5091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2184 1184
Size: 1000 5091
AlwNI CAG_nnn'CTG
Cuts at: 2089 3377 3834 5406 2089
Size: 1288 457 1572 2774
Fragments arranged by size:
2774 1572 1288 457
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3291 3400 3626 3778 5887 1493
Size: 1798 109 226 152 2109 1697
Fragments arranged by size:
2109 1798 1697 226 152 109
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 3416 1870
Size: 73 1473 4545
Fragments arranged by size:
4545 1473 73
BamHI G'GATC_C
Cuts at: 2772 3421 3788 2772
Size: 649 367 5075
Fragments arranged by size:
5075 649 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 3412 4867 618
Size: 325 217 627 381 1244 1455 1842
Fragments arranged by size:
1842 1455 1244 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2445 3410 1391
Size: 1054 965 4072
Fragments arranged by size:
4072 1054 965
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 2786 928
Size: 971 602 285 4233
Fragments arranged by size:
4233 971 602 285
BbvCI CC'TCA_GC
Cuts at: 2115 2115
Size: 6091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3260 3983 5193 3260
Size: 723 1210 4158
Fragments arranged by size:
4158 1210 723
BclI T'GATC_A
Cuts at: 1548 2391 1548
Size: 843 5248
BfrBI ATG'CAT
Cuts at: 3206 3206
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2177 4702 136
Size: 107 122 71 631 605 505 2525 1525
Fragments arranged by size:
2525 1525 631 605 505 122 107 71
BglII A'GATC_T
Cuts at: 2895 6086 2895
Size: 3191 2900
BlpI GC'TnA_GC
Cuts at: 2016 3508 2016
Size: 1492 4599
Bme1580I G_kGCm'C
Cuts at: 1790 2173 2369 4152 5308 1790
Size: 383 196 1783 1156 2573
Fragments arranged by size:
2573 1783 1156 383 196
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2887 3188 3822
Size: 416 53 83 399 24 1470 301 634
Cuts at: 3822 4780 442
Size: 958 1753
Fragments arranged by size:
1753 1470 958 634 416 399 301 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4751 1239
Size: 3512 2579
Bpu10I CC'TnA_GC
Cuts at: 2115 2542 2115
Size: 427 5664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2233 2957 4216 5081 5379 2233
Size: 724 1259 865 298 2945
Fragments arranged by size:
2945 1259 865 724 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3303 4754 882
Size: 617 1804 1451 2219
Fragments arranged by size:
2219 1804 1451 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 2624 6085 2624
Size: 3461 2630
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3302 3332 1178
Size: 30 165 30 1899 30 3937
Fragments arranged by size:
3937 1899 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 1186
Size: 847 5244
BseYI C'CCAG_C
Cuts at: 2260 2267 5294 5790 2260
Size: 7 3027 496 2561
Fragments arranged by size:
3027 2561 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2656 1163
Size: 573 920 4598
Fragments arranged by size:
4598 920 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2181 2376 3541 4302 5330 1540
Size: 275 186 180 195 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 195 186 180
BsiHKAI G_wGCw'C
Cuts at: 2148 2934 4152 4237 5308 2148
Size: 786 1218 85 1071 2931
Fragments arranged by size:
2931 1218 1071 786 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2173 2369 2445 2934 3410 4152
Size: 399 358 25 196 76 489 476 742
Cuts at: 4152 4237 5308 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 476 399 358 196
85 76 25
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2895 3446 844
Size: 2051 551 3489
Fragments arranged by size:
3489 2051 551
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2694 3159 4586 4760 65
Size: 1183 1446 465 1427 174 1396
Fragments arranged by size:
1446 1427 1396 1183 465 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2375 4735 4922 1700
Size: 110 565 2360 187 2869
Fragments arranged by size:
2869 2360 565 187 110
BsrGI T'GTAC_A
Cuts at: 96 2846 96
Size: 2750 3341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3377 794
Size: 2583 3508
BstBI TT'CG_AA
Cuts at: 3022 4895 3022
Size: 1873 4218
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstXI CCAn_nnnn'nTGG
Cuts at: 2303 3334 2303
Size: 1031 5060
BstYI r'GATC_y
Cuts at: 1972 2772 2895 3421 3788 4173 4190 5642 5728
Size: 800 123 526 367 385 17 1452 86
Cuts at: 5728 5740 6086 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 800 526 385 367 346 123
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2190 3321 513
Size: 552 1125 1131 3283
Fragments arranged by size:
3283 1131 1125 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 2689 1554
Size: 1135 4956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2397 2594
Size: 54 1425 50 275 18 348 219 197
Cuts at: 2594 3324 3538 4427 6056 8
Size: 730 214 889 1629 43
Fragments arranged by size:
1629 1425 889 730 348 275 219 214
197 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2178 3538 1537
Size: 275 366 1360 4090
Fragments arranged by size:
4090 1360 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2450 2785 3265 4021 1353
Size: 1097 335 480 756 3423
Fragments arranged by size:
3423 1097 756 480 335
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2021 4678 5052 5198 387
Size: 1064 138 432 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 432 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2766 2912 3220 4154 4751 5538 5806
Size: 393 1134 146 308 934 597 787 268
Cuts at: 5806 1239
Size: 1524
Fragments arranged by size:
1524 1134 934 787 597 393 308 268
146
EcoRI G'AATT_C
Cuts at: 3291 3400 3778 3291
Size: 109 378 5604
Fragments arranged by size:
5604 378 109
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2123 2205 5238 1678
Size: 445 82 3033 2531
Fragments arranged by size:
3033 2531 445 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2365 2397 2671 2703 2946
Size: 32 135 32 793 32 274 32 243
Cuts at: 2946 2978 3302 3334 4732 4764 4806 4838 1373
Size: 32 324 32 1398 32 42 32 2626
Fragments arranged by size:
2626 1398 793 324 274 243 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3435 3681 669
Size: 988 281 349 1148 246 3079
Fragments arranged by size:
3079 1148 988 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 2224 3690 2224
Size: 1466 4625
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 2396 2891 3288 5205 5389 1305
Size: 1035 56 495 397 1917 184 2007
Fragments arranged by size:
2007 1917 1035 495 397 184 56
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2596 10
Size: 54 1425 343 764 3505
Fragments arranged by size:
3505 1425 764 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 2301 2408 2468 2489 2942 3035
Size: 648 1000 135 107 60 21 453 93
Cuts at: 3035 4049 4408 4567 518
Size: 1014 359 159 2042
Fragments arranged by size:
2042 1014 1000 648 453 359 159 135
107 93 60 21
MspA1I CmG'CkG
Cuts at: 1606 2098 2166 3183 3507 4184 5332 5577 1606
Size: 492 68 1017 324 677 1148 245 2120
Fragments arranged by size:
2120 1148 1017 677 492 324 245 68
NcoI C'CATG_G
Cuts at: 513 1065 2190 513
Size: 552 1125 4414
Fragments arranged by size:
4414 1125 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NsiI A_TGCA'T
Cuts at: 3208 3208
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2650 2989 3449 4994 1132
Size: 97 1421 339 460 1545 2229
Fragments arranged by size:
2229 1545 1421 460 339 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 2646 4990 2646
Size: 2344 3747
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 3183 3183
Size: 6091
RsrII CG'GwC_CG
Cuts at: 2327 2327
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SapI GCTCTTCn'nnn_
Cuts at: 2450 2450
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SexAI A'CCwGG_T
Cuts at: 2870 2870
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 3439 4574 5255 5446 826
Size: 219 2394 1135 681 191 1471
Fragments arranged by size:
2394 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2248 2972 4195 5096 5358 820
Size: 197 926 305 724 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 724 305 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 2989 3449 1132
Size: 1857 460 3774
Fragments arranged by size:
3774 1857 460
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 2720 1517
Size: 1203 4888
StyI C'CwwG_G
Cuts at: 513 1065 2190 2238 3108 3530 513
Size: 552 1125 48 870 422 3074
Fragments arranged by size:
3074 1125 870 552 422 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2012 2281 4118 4135 4288 4473 4918
Size: 1255 432 269 1837 17 153 185 445
Cuts at: 4918 325
Size: 1498
Fragments arranged by size:
1837 1498 1255 445 432 269 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2846 4337 6049
Size: 274 80 33 51 1443 869 1491 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
1712 1491 1443 869 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2407 2431 2874 3040 2407
Size: 24 443 166 5458
Fragments arranged by size:
5458 443 166 24
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
SacI SacII SanDI SfiI SfoI SrfI SwaI