Restriction map for
pFN21AB6695
(Circular) (Six-base) MAPSORT of: pFN21AB6695.seq Check: 7747 from: 1 to: 5992
pFN21AB6695 5992 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3112 3112
Size: 5992
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 3015 4055 5439 5707 1632
Size: 734 649 1040 1384 268 1917
Fragments arranged by size:
1917 1384 1040 734 649 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 2131 2647 4721 2131
Size: 516 2074 3402
Fragments arranged by size:
3402 2074 516
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2463 2837 1184
Size: 1279 374 4339
Fragments arranged by size:
4339 1279 374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 5960
AlwNI CAG_nnn'CTG
Cuts at: 2182 3735 5307 2182
Size: 1553 1572 2867
Fragments arranged by size:
2867 1572 1553
ApaI G_GGCC'C
Cuts at: 2282 3175 2282
Size: 893 5099
ApaLI G'TGCA_C
Cuts at: 2415 4049 5205 2415
Size: 1634 1156 3202
Fragments arranged by size:
3202 1634 1156
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 1870
Size: 73 1374 4545
Fragments arranged by size:
4545 1374 73
BamHI G'GATC_C
Cuts at: 3232 3322 3689 3232
Size: 90 367 5535
Fragments arranged by size:
5535 367 90
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3124 3313 4768 618
Size: 325 217 627 1337 189 1455 1842
Fragments arranged by size:
1842 1455 1337 627 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2149 2282 2594 3069 3175 3311 1391
Size: 758 133 312 475 106 136 4072
Fragments arranged by size:
4072 758 475 312 136 133 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 928
Size: 971 504 4517
Fragments arranged by size:
4517 971 504
BbvCI CC'TCA_GC
Cuts at: 2433 2433
Size: 5992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 2730 1548
Size: 1182 4810
BfrBI ATG'CAT
Cuts at: 2814 2814
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3073 4603 136
Size: 107 122 71 631 605 1401 1530 1525
Fragments arranged by size:
1530 1525 1401 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 2282 2419 2480 3129 3175 4053 5209 1790
Size: 492 137 61 649 46 878 1156 2573
Fragments arranged by size:
2573 1156 878 649 492 137 61 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3723 4681 442
Size: 416 53 83 399 24 2306 958 1753
Fragments arranged by size:
2306 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2240 2277 4652 1239
Size: 842 159 37 2375 2579
Fragments arranged by size:
2579 2375 842 159 37
Bpu10I CC'TnA_GC
Cuts at: 2433 3017 2433
Size: 584 5408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 3224 4117 4982 5280 2363
Size: 861 893 865 298 3075
Fragments arranged by size:
3075 893 865 861 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3176 4655 882
Size: 617 1677 1479 2219
Fragments arranged by size:
2219 1677 1479 617
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3799 4181 4816 275
Size: 53 83 186 936 2266 382 635 1451
Fragments arranged by size:
2266 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2253 2283 2409
Size: 30 165 30 597 30 223 30 126
Cuts at: 2409 2439 3273 3303 1178
Size: 30 834 30 3867
Fragments arranged by size:
3867 834 597 223 165 126 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2274 2449 2450 2501 3290 1186
Size: 1088 175 1 51 789 3888
Fragments arranged by size:
3888 1088 789 175 51 1
BseYI C'CCAG_C
Cuts at: 2749 5195 5691 2749
Size: 2446 496 3050
Fragments arranged by size:
3050 2446 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2059 3116 1163
Size: 573 323 1057 4039
Fragments arranged by size:
4039 1057 573 323
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2419 3051 4053 4138 5209 2108
Size: 311 632 1002 85 1071 2891
Fragments arranged by size:
2891 1071 1002 632 311 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2149 2282 2419 2480 2594 3051
Size: 399 318 41 133 137 61 114 457
Cuts at: 3051 3069 3129 3175 3311 4053 4138 5209 1391
Size: 18 60 46 136 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 399 318 137 136
133 114 85 61 60 46 41 18
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 3881 5611 3881
Size: 1730 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3112 3347 844
Size: 2268 235 3489
Fragments arranged by size:
3489 2268 235
BsrBI CCG'CTC
Cuts at: 1394 1823 3879 1394
Size: 429 2056 3507
Fragments arranged by size:
3507 2056 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4487 4661 65
Size: 1183 3239 174 1396
Fragments arranged by size:
3239 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3117 4636 4823 1700
Size: 110 1307 1519 187 2869
Fragments arranged by size:
2869 1519 1307 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 4052 5064 4052
Size: 1012 4980
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2929 794
Size: 2135 3857
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 2557 2557
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 2179 3232 3322 3689 4074 4091 5543 5629
Size: 207 1053 90 367 385 17 1452 86
Cuts at: 5629 5641 5987 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1053 385 367 346 207 90
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2370 2529 2370
Size: 159 5833
BtgI C'CryG_G
Cuts at: 513 1065 2004 2556 513
Size: 552 939 552 3949
Fragments arranged by size:
3949 939 552 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5253
Fragments arranged by size:
5253 683 56
BtsI GCAGTG_nn'
Cuts at: 2359 2550 3508 4302 4322 2359
Size: 191 958 794 20 4029
Fragments arranged by size:
4029 958 794 191 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2150 2185 2255 2290 529
Size: 35 1586 35 70 35 4231
Fragments arranged by size:
4231 1586 70 35 35 35
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3439 4328 5957
Size: 54 1425 50 275 18 1609 889 1629
Cuts at: 5957 8
Size: 43
Fragments arranged by size:
1629 1609 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2529 2991 3922 1353
Size: 710 466 462 931 3423
Fragments arranged by size:
3423 931 710 466 462
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4579 4953 5099 387
Size: 1064 138 2990 374 146 1280
Fragments arranged by size:
2990 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2240 2277 2366 3015 4055 4652
Size: 393 449 159 37 89 649 1040 597
Cuts at: 4652 5439 5707 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1040 787 649 597 449 393 268
159 89 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2373 2373
Size: 5992
EcoO109I rG'GnC_Cy
Cuts at: 2278 2279 3171 2278
Size: 1 892 5099
Fragments arranged by size:
5099 892 1
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 5139 1678
Size: 3461 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2576 2608 2633
Size: 32 135 32 545 32 427 32 25
Cuts at: 2633 2665 3273 3305 4633 4665 4707 4739 1373
Size: 32 608 32 1328 32 42 32 2626
Fragments arranged by size:
2626 1328 608 545 427 135 42 32
32 32 32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2136 3336 3582 669
Size: 988 281 198 1200 246 3079
Fragments arranged by size:
3079 1200 988 281 246 198
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5106 5290 1305
Size: 3801 184 2007
Fragments arranged by size:
3801 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4170
Fragments arranged by size:
4170 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 2837 2868 3950 4309 4468 518
Size: 648 1389 282 31 1082 359 159 2042
Fragments arranged by size:
2042 1389 1082 648 359 282 159 31
MspA1I CmG'CkG
Cuts at: 1606 3408 4085 5233 5478 1606
Size: 1802 677 1148 245 2120
Fragments arranged by size:
2120 1802 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2556 513
Size: 552 939 552 3949
Fragments arranged by size:
3949 939 552 552
NdeI CA'TA_TG
Cuts at: 387 2152 387
Size: 1765 4227
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NsiI A_TGCA'T
Cuts at: 2816 2816
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2818 2932 3147 3350 4895 1132
Size: 97 1589 114 215 203 1545 2229
Fragments arranged by size:
2229 1589 1545 215 203 114 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5992
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2000 2032 4061 4093 5574 5606 2000
Size: 32 2029 32 1481 32 2386
Fragments arranged by size:
2386 2029 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2229 1613
Size: 616 5376
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PspOMI G'GGCC_C
Cuts at: 2278 3171 2278
Size: 893 5099
PstI C_TGCA'G
Cuts at: 830 3344 830
Size: 2514 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SexAI A'CCwGG_T
Cuts at: 3108 3108
Size: 5992
SfcI C'TryA_G
Cuts at: 826 1045 2165 3340 4475 5156 5347 826
Size: 219 1120 1175 1135 681 191 1471
Fragments arranged by size:
1471 1175 1135 1120 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 3203 4096 4997 5259 820
Size: 197 926 399 861 893 901 262 1553
Fragments arranged by size:
1553 926 901 893 861 399 262 197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 2818 2932 3147 3350 1132
Size: 1686 114 215 203 3774
Fragments arranged by size:
3774 1686 215 203 114
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 2379 1517
Size: 862 5130
StyI C'CwwG_G
Cuts at: 513 1065 2004 2556 3167 3431 513
Size: 552 939 552 611 264 3074
Fragments arranged by size:
3074 939 611 552 552 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 2402 2951 3082 4019 4036 4189
Size: 1255 723 99 549 131 937 17 153
Cuts at: 4189 4374 4819 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 937 723 549 445 185 153
131 99 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3207 4238 5950
Size: 274 80 33 51 1443 1230 1031 1712
Cuts at: 5950 96
Size: 138
Fragments arranged by size:
1712 1443 1230 1031 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2253 2285 2409 2441 5952 5984 2085
Size: 32 136 32 124 32 3511 32 2093
Fragments arranged by size:
3511 2093 136 124 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 2482 3328 2482
Size: 846 5146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2166 2252 2166
Size: 86 5906
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 2379 4121 2379
Size: 1742 4250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI