Restriction map for
pFN21AB6688
(Circular) (Six-base) MAPSORT of: pFN21AB6688.seq Check: 4618 from: 1 to: 6079
pFN21AB6688 6079 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2890 3889 278
Size: 53 83 186 936 1354 999 2468
Fragments arranged by size:
2468 1354 999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 3422 1937
Size: 1170 315 4594
Fragments arranged by size:
4594 1170 315
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2816 4142 5526 5794 1632
Size: 1184 1326 1384 268 1917
Fragments arranged by size:
1917 1384 1326 1184 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 4978 5955 1203
Size: 3775 977 1327
Fragments arranged by size:
3775 1327 977
AgeI A'CCGG_T
Cuts at: 3268 4910 3268
Size: 1642 4437
AhdI GACnn_n'nnGTC
Cuts at: 2318 4808 2318
Size: 2490 3589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 3822 5394 3822
Size: 1572 4507
ApaLI G'TGCA_C
Cuts at: 2042 4136 5292 2042
Size: 2094 1156 2829
Fragments arranged by size:
2829 2094 1156
ApoI r'AATT_y
Cuts at: 1493 2144 2641 3123 3322 3388 3614 3766 5875
Size: 651 497 482 199 66 226 152 2109
Cuts at: 5875 1493
Size: 1697
Fragments arranged by size:
2109 1697 651 497 482 226 199 152
66
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3404 1870
Size: 73 1461 4545
Fragments arranged by size:
4545 1461 73
AvrII C'CTAG_G
Cuts at: 2891 2891
Size: 6079
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2897 2976 3400 4855 618
Size: 325 217 627 1110 79 424 1455 1842
Fragments arranged by size:
1842 1455 1110 627 424 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2090 3300 3398 1391
Size: 699 1210 98 4072
Fragments arranged by size:
4072 1210 699 98
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2608 2642 4270 4304 1903
Size: 34 671 34 1628 34 3678
Fragments arranged by size:
3678 1628 671 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 2226 2967 1548
Size: 678 741 4660
Fragments arranged by size:
4660 741 678
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4690 136
Size: 107 122 71 631 605 3018 1525
Fragments arranged by size:
3018 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2387 2900 2981 4140 5296 1790
Size: 256 341 513 81 1159 1156 2573
Fragments arranged by size:
2573 1159 1156 513 341 256 81
BmgBI CAC'GTC
Cuts at: 1318 2622 1318
Size: 1304 4775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2084 3810 4768
Size: 416 53 83 399 24 667 1726 958
Cuts at: 4768 442
Size: 1753
Fragments arranged by size:
1753 1726 958 667 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3331 4739 1239
Size: 2092 1408 2579
Fragments arranged by size:
2579 2092 1408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4204 5069 5367 4204
Size: 865 298 4916
Fragments arranged by size:
4916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4742 882
Size: 617 3243 2219
Fragments arranged by size:
3243 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2474 2703 3870 493
Size: 711 1270 229 1167 2702
Fragments arranged by size:
2702 1270 1167 711 229
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2887 3886 4268 4903
Size: 53 83 186 936 1354 999 382 635
Cuts at: 4903 275
Size: 1451
Fragments arranged by size:
1451 1354 999 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3268 4512 4910 5184 5331 5742 3268
Size: 1244 398 274 147 411 3605
Fragments arranged by size:
3605 1244 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5854
Fragments arranged by size:
5854 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6079
BseYI C'CCAG_C
Cuts at: 5282 5778 5282
Size: 496 5583
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2240 2735 1163
Size: 573 504 495 4507
Fragments arranged by size:
4507 573 504 495
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2820 3529 4290 5318 1540
Size: 275 186 819 709 761 1028 2301
Fragments arranged by size:
2301 1028 819 761 709 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2631 4140 4225 5296 2046
Size: 585 1509 85 1071 2829
Fragments arranged by size:
2829 1509 1071 585 85
BsmI GAATG_Cn'
Cuts at: 2026 2029 3137 3590 3683 2026
Size: 3 1108 453 93 4422
Fragments arranged by size:
4422 1108 453 93 3
BsmBI CGTCTCn'nnnn_
Cuts at: 2878 2878
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2090 2387 2631 2900 2981 3300
Size: 399 256 44 297 244 269 81 319
Cuts at: 3300 3398 4140 4225 5296 1391
Size: 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 297 269 256
244 98 85 81 44
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 2199 2520 3968 5698 2199
Size: 321 1448 1730 2580
Fragments arranged by size:
2580 1730 1448 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3434 844
Size: 2590 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 4574 4748 65
Size: 1183 1044 2282 174 1396
Fragments arranged by size:
2282 1396 1183 1044 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3268 4723 4910 1700
Size: 110 1458 1455 187 2869
Fragments arranged by size:
2869 1458 1455 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 2933 4883 2933
Size: 1950 4129
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074
Size: 1437 367 385 17 1452 86 12 346
Cuts at: 6074 1972
Size: 1977
Fragments arranged by size:
1977 1452 1437 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3108 3108
Size: 6079
Bsu36I CC'TnA_GG
Cuts at: 2015 2015
Size: 6079
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 3213 3379 3595 4389 4409 3213
Size: 166 216 794 20 4883
Fragments arranged by size:
4883 794 216 166 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DraIII CAC_nnn'GTG
Cuts at: 2622 2622
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2168 2363 2755
Size: 54 1425 50 275 18 338 195 392
Cuts at: 2755 2817 3526 4415 6044 8
Size: 62 709 889 1629 43
Fragments arranged by size:
1629 1425 889 709 392 338 275 195
62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2817 3526 1537
Size: 275 1005 709 4090
Fragments arranged by size:
4090 1005 709 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2820 4009 1353
Size: 1467 1189 3423
Fragments arranged by size:
3423 1467 1189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2816 3331 4142 4739 5526 5794 1239
Size: 393 1184 515 811 597 787 268 1524
Fragments arranged by size:
1524 1184 811 787 597 515 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2658 2658
Size: 6079
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2946 2946
Size: 6079
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 5226 1678
Size: 3548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 4720 4752 4794
Size: 32 135 32 732 32 2384 32 42
Cuts at: 4794 4826 1373
Size: 32 2626
Fragments arranged by size:
2626 2384 732 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3244 3423 3669 669
Size: 988 281 1306 179 246 3079
Fragments arranged by size:
3079 1306 988 281 246 179
HindIII A'AGCT_T
Cuts at: 748 2736 2928 3047 748
Size: 1988 192 119 3780
Fragments arranged by size:
3780 1988 192 119
HpaI GTT'AAC
Cuts at: 3244 3669 3244
Size: 425 5654
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3168 5193 5377 1305
Size: 1863 2025 184 2007
Fragments arranged by size:
2025 2007 1863 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2170 2365 10
Size: 54 1425 343 338 195 3724
Fragments arranged by size:
3724 1425 343 338 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2573 3261 4037 4396 4555 518
Size: 648 1056 351 688 776 359 159 2042
Fragments arranged by size:
2042 1056 776 688 648 359 351 159
MspA1I CmG'CkG
Cuts at: 1606 2683 3495 4172 5320 5565 1606
Size: 1077 812 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 812 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 3437 4982 1132
Size: 97 2208 1545 2229
Fragments arranged by size:
2229 2208 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2658 2658
Size: 6079
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3165 3649 3165
Size: 484 5595
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 6047
PstI C_TGCA'G
Cuts at: 830 3431 830
Size: 2601 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SfcI C'TryA_G
Cuts at: 826 1045 2100 2550 3427 4562 5243 5434 826
Size: 219 1055 450 877 1135 681 191 1471
Fragments arranged by size:
1471 1135 1055 877 681 450 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4183 5084 5346 820
Size: 197 926 2240 901 262 1553
Fragments arranged by size:
2240 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2474 2703 3870 493
Size: 1981 229 1167 2702
Fragments arranged by size:
2702 1981 1167 229
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 2998 4003 5
Size: 47 2946 1005 2081
Fragments arranged by size:
2946 2081 1005 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2004 2891 2941 3518 513
Size: 552 939 887 50 577 3074
Fragments arranged by size:
3074 939 887 577 552 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4325 6037 96
Size: 274 80 33 51 1443 2348 1712 138
Fragments arranged by size:
2348 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3358 3390 6039 6071 3358
Size: 32 2649 32 3366
Fragments arranged by size:
3366 2649 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2781 1316
Size: 1465 4614
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 2929 3048 4208 2929
Size: 119 1160 4800
Fragments arranged by size:
4800 1160 119
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2888 3887 276
Size: 53 83 186 936 1354 999 2468
Fragments arranged by size:
2468 1354 999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI