Restriction map for
pFN21AB6687
(Circular) (Six-base) MAPSORT of: pFN21AB6687.seq Check: 5080 from: 1 to: 5986
pFN21AB6687 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 2619 4113 4486 1563
Size: 1056 1494 373 3063
Fragments arranged by size:
3063 1494 1056 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3009 4049 5433 5701 1632
Size: 1377 1040 1384 268 1917
Fragments arranged by size:
1917 1384 1377 1040 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 2629 4715 2629
Size: 2086 3900
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2365 1184
Size: 1181 4805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2722 2754 2722
Size: 32 5954
AlwNI CAG_nnn'CTG
Cuts at: 2410 3263 3729 5301 2410
Size: 853 466 1572 3095
Fragments arranged by size:
3095 1572 853 466
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2086 2303 2744 3295 3521 3673 5782 1493
Size: 593 217 441 551 226 152 2109 1697
Fragments arranged by size:
2109 1697 593 551 441 226 217 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2585 3311 1870
Size: 73 642 726 4545
Fragments arranged by size:
4545 726 642 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3307 4762 618
Size: 325 217 627 1520 1455 1842
Fragments arranged by size:
1842 1520 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2113 2334 3305 1391
Size: 722 221 971 4072
Fragments arranged by size:
4072 971 722 221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3195 928
Size: 971 1296 3719
Fragments arranged by size:
3719 1296 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 2658 2698 3153 1548
Size: 1110 40 455 4381
Fragments arranged by size:
4381 1110 455 40
BfrBI ATG'CAT
Cuts at: 2921 2921
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 2882 4597
Size: 107 122 71 631 605 538 672 1715
Cuts at: 4597 136
Size: 1525
Fragments arranged by size:
1715 1525 672 631 605 538 122 107
71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 2174 3403 2174
Size: 1229 4757
Bme1580I G_kGCm'C
Cuts at: 1790 4047 5203 1790
Size: 2257 1156 2573
Fragments arranged by size:
2573 2257 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2460 3717 4675
Size: 416 53 83 399 24 1043 1257 958
Cuts at: 4675 442
Size: 1753
Fragments arranged by size:
1753 1257 1043 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4646 1239
Size: 3407 2579
Bpu10I CC'TnA_GC
Cuts at: 2104 2104
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2097 4111 4976 5274 2097
Size: 2014 865 298 2809
Fragments arranged by size:
2809 2014 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 2343 5980 2343
Size: 3637 2349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2552 3793 4175 4810
Size: 53 83 186 936 1019 1241 382 635
Cuts at: 4810 275
Size: 1451
Fragments arranged by size:
1451 1241 1019 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3110 1186
Size: 1924 4062
BseYI C'CCAG_C
Cuts at: 2845 3263 5189 5685 2845
Size: 418 1926 496 3146
Fragments arranged by size:
3146 1926 496 418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3165 1163
Size: 573 1429 3984
Fragments arranged by size:
3984 1429 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2113 2334 2584 4047 4132 5203 2113
Size: 221 250 1463 85 1071 2896
Fragments arranged by size:
2896 1463 1071 250 221 85
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2334 2584 3305 4047 4132 5203
Size: 399 323 221 250 721 742 85 1071
Cuts at: 5203 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 721 399 323 250 221
85
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 2377 2748 3875 5605 2377
Size: 371 1127 1730 2758
Fragments arranged by size:
2758 1730 1127 371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3341 844
Size: 2497 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 2908 2908
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2931 3012 3217 3316 3683 4068 4085 5537
Size: 959 81 205 99 367 385 17 1452
Cuts at: 5537 5623 5635 5981 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 959 385 367 346 205 99
86 81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5434
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 3156 3502 4296 4316 3156
Size: 346 794 20 4826
Fragments arranged by size:
4826 794 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 2091 3290 2091
Size: 1199 4787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2520 3433 4322
Size: 54 1425 50 275 18 690 913 889
Cuts at: 4322 5951 8
Size: 1629 43
Fragments arranged by size:
1629 1425 913 889 690 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 3048 3916 1353
Size: 1478 217 868 3423
Fragments arranged by size:
3423 1478 868 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2111 2332 2111
Size: 221 5765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3009 4049 4646 5433 5701 1239
Size: 393 1377 1040 597 787 268 1524
Fragments arranged by size:
1524 1377 1040 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2744 3295 3673 2744
Size: 551 378 5057
Fragments arranged by size:
5057 551 378
EcoRV GAT'ATC
Cuts at: 2081 2081
Size: 5986
FspI TGC'GCA
Cuts at: 2128 4492 2128
Size: 2364 3622
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2615 2643 2647 2675 2882
Size: 32 135 32 1043 28 4 28 207
Cuts at: 2882 2914 4627 4659 4701 4733 1373
Size: 32 1713 32 42 32 2626
Fragments arranged by size:
2626 1713 1043 207 135 42 32 32
32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2623 3213 3330 3576 669
Size: 988 281 685 590 117 246 3079
Fragments arranged by size:
3079 988 685 590 281 246 117
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2977 3245 5100 5284 1305
Size: 1672 268 1855 184 2007
Fragments arranged by size:
2007 1855 1672 268 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2522 10
Size: 54 1425 343 690 3474
Fragments arranged by size:
3474 1425 690 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 3944 4303 4462 518
Size: 648 1158 1620 359 159 2042
Fragments arranged by size:
2042 1620 1158 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2849 3263 3402 4079 5227 5472 1606
Size: 1243 414 139 677 1148 245 2120
Fragments arranged by size:
2120 1243 1148 677 414 245 139
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5434
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NsiI A_TGCA'T
Cuts at: 2923 2923
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 3344 4889 1132
Size: 97 2115 1545 2229
Fragments arranged by size:
2229 2115 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2211 2243 4055 4087 5568 5600 2211
Size: 32 1812 32 1481 32 2597
Fragments arranged by size:
2597 1812 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2514 3246 1613
Size: 901 732 4353
Fragments arranged by size:
4353 901 732
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 2533 3338 830
Size: 1703 805 3478
Fragments arranged by size:
3478 1703 805
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2849 3263 2849
Size: 414 5572
SacI G_AGCT'C
Cuts at: 2113 2334 2113
Size: 221 5765
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2097 2215 2529 2575 2937 3280 3334
Size: 219 1052 118 314 46 362 343 54
Cuts at: 3334 4469 5150 5341 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1052 681 362 343 314 219
191 118 54 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2112 4090 4991 5253 820
Size: 197 926 169 1978 901 262 1553
Fragments arranged by size:
1978 1553 926 901 262 197 169
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2596 3425 513
Size: 552 1531 829 3074
Fragments arranged by size:
3074 1531 829 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 2628 4013 4030 4183 4368 4813
Size: 1255 924 124 1385 17 153 185 445
Cuts at: 4813 325
Size: 1498
Fragments arranged by size:
1498 1385 1255 924 445 185 153 124
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2997 3029 5946 5978 2997
Size: 32 2917 32 3005
Fragments arranged by size:
3005 2917 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3250 3322 3250
Size: 72 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 2276 2304 2825 4115 2276
Size: 28 521 1290 4147
Fragments arranged by size:
4147 1290 521 28
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI