Restriction map for
pFN21AB6686
(Circular) (Six-base) MAPSORT of: pFN21AB6686.seq Check: 7748 from: 1 to: 6079
pFN21AB6686 6079 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 3422 1937
Size: 990 495 4594
Fragments arranged by size:
4594 990 495
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 2557 4142 5526 5794 1632
Size: 815 110 1585 1384 268 1917
Fragments arranged by size:
1917 1585 1384 815 268 110
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 3191 4978 5955 1203
Size: 1988 1787 977 1327
Fragments arranged by size:
1988 1787 1327 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 3822 5394 3822
Size: 1572 4507
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 2750 3388 3614 3766 5875 1493
Size: 1257 638 226 152 2109 1697
Fragments arranged by size:
2109 1697 1257 638 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 3404 1870
Size: 73 1287 174 4545
Fragments arranged by size:
4545 1287 174 73
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2421 3400 4855 618
Size: 325 217 627 634 979 1455 1842
Fragments arranged by size:
1842 1455 979 634 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2027 2438 2490 2658 2976 3135 3398 1391
Size: 636 411 52 168 318 159 263 4072
Fragments arranged by size:
4072 636 411 318 263 168 159 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5108
BbvCI CC'TCA_GC
Cuts at: 2880 3268 2880
Size: 388 5691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3211 3245 4270 4304 1903
Size: 34 1274 34 1025 34 3678
Fragments arranged by size:
3678 1274 1025 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2558 4690 136
Size: 107 122 71 631 605 886 2132 1525
Fragments arranged by size:
2132 1525 886 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3020 3125 3496 3020
Size: 105 371 5603
Fragments arranged by size:
5603 371 105
Bme1580I G_kGCm'C
Cuts at: 1790 4140 5296 1790
Size: 2350 1156 2573
Fragments arranged by size:
2573 2350 1156
BmgBI CAC'GTC
Cuts at: 1318 3194 1318
Size: 1876 4203
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3810 4768 442
Size: 416 53 83 399 24 2393 958 1753
Fragments arranged by size:
2393 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2445 2714 1056
Size: 1389 269 4421
Fragments arranged by size:
4421 1389 269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2399 2420 2558 2699 3017 3059 3218
Size: 1142 18 21 138 141 318 42 159
Cuts at: 3218 4739 1239
Size: 1521 2579
Fragments arranged by size:
2579 1521 1142 318 159 141 138 42
21 18
Bpu10I CC'TnA_GC
Cuts at: 2448 2880 2975 3268 2448
Size: 432 95 293 5259
Fragments arranged by size:
5259 432 293 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4204 5069 5367 2564
Size: 1640 865 298 3276
Fragments arranged by size:
3276 1640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4742 882
Size: 617 3243 2219
Fragments arranged by size:
3243 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3870 493
Size: 711 2666 2702
Fragments arranged by size:
2702 2666 711
BsaBI GATnn'nnATC
Cuts at: 2687 6073 2687
Size: 3386 2693
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3243 3886 4268 4903
Size: 53 83 186 936 1710 643 382 635
Cuts at: 4903 275
Size: 1451
Fragments arranged by size:
1710 1451 936 643 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4512 4910 5184 5331 5742 4512
Size: 398 274 147 411 4849
Fragments arranged by size:
4849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2133 2163 1178
Size: 30 165 30 730 30 5094
Fragments arranged by size:
5094 730 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 2091 2291 2396 2753 1186
Size: 831 74 200 105 357 4512
Fragments arranged by size:
4512 831 357 200 105 74
BseYI C'CCAG_C
Cuts at: 2071 2129 2437 3015 3120 5282 5778 2071
Size: 58 308 578 105 2162 496 2372
Fragments arranged by size:
2372 2162 578 496 308 105 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2925 1163
Size: 573 1189 4317
Fragments arranged by size:
4317 1189 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 3529 4290 5318 1540
Size: 275 186 343 1185 761 1028 2301
Fragments arranged by size:
2301 1185 1028 761 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 2027 2490 2658 2976 3135 4140 4225 5296 2027
Size: 463 168 318 159 1005 85 1071 2810
Fragments arranged by size:
2810 1071 1005 463 318 168 159 85
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
BsmBI CGTCTCn'nnnn_
Cuts at: 3050 3050
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2438 2490 2658 2976 3135 3398
Size: 399 237 411 52 168 318 159 263
Cuts at: 3398 4140 4225 5296 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 318 263 237
168 159 85 52
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3434 844
Size: 2590 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2452 3966 1394
Size: 429 629 1514 3507
Fragments arranged by size:
3507 1514 629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 4574 4748 65
Size: 1183 1069 2257 174 1396
Fragments arranged by size:
2257 1396 1183 1069 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4723 4910 1700
Size: 110 2913 187 2869
Fragments arranged by size:
2913 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6079
BssHII G'CGCG_C
Cuts at: 1925 2371 1925
Size: 446 5633
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2409 2499 3090 3409 3776 4161 4178 5630
Size: 437 90 591 319 367 385 17 1452
Cuts at: 5630 5716 5728 6074 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 591 437 385 367 346 319
90 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2083 2853 513
Size: 552 1018 770 3739
Fragments arranged by size:
3739 1018 770 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 2934 3145 3595 4389 4409 2934
Size: 211 450 794 20 4604
Fragments arranged by size:
4604 794 450 211 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2770 3526 4415
Size: 54 1425 50 275 18 940 756 889
Cuts at: 4415 6044 8
Size: 1629 43
Fragments arranged by size:
1629 1425 940 889 756 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3526 1537
Size: 275 1714 4090
Fragments arranged by size:
4090 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 2700 2730 4009 1353
Size: 1125 222 30 1279 3423
Fragments arranged by size:
3423 1279 1125 222 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2003 4666 5040 5186 387
Size: 1064 138 414 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 414 374 146 138
EcoICRI GAG'CTC
Cuts at: 2025 2488 2656 2974 3133 2025
Size: 463 168 318 159 4971
Fragments arranged by size:
4971 463 318 168 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2399 2420 2447 2557 2558 2699
Size: 393 749 18 21 27 110 1 141
Cuts at: 2699 3017 3059 3218 4142 4739 5526 5794 1239
Size: 318 42 159 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 749 597 393 318 268
159 141 110 42 27 21 18 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2363 2381 3230 2363
Size: 18 849 5212
Fragments arranged by size:
5212 849 18
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 5226 1678
Size: 3548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2996 3028 4720 4752 4794
Size: 32 135 32 1424 32 1692 32 42
Cuts at: 4794 4826 1373
Size: 32 2626
Fragments arranged by size:
2626 1692 1424 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 2779 3423 3669 669
Size: 988 281 337 504 644 246 3079
Fragments arranged by size:
3079 988 644 504 337 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5193 5377 1305
Size: 3888 184 2007
Fragments arranged by size:
3888 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4257
Fragments arranged by size:
4257 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2939 4037 4396 4555 518
Size: 648 1773 1098 359 159 2042
Fragments arranged by size:
2042 1773 1098 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2129 3019 3082 3124 3495 4172 5320
Size: 465 58 890 63 42 371 677 1148
Cuts at: 5320 5565 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 890 677 465 371 245 63
58 42
NcoI C'CATG_G
Cuts at: 513 1065 2853 513
Size: 552 1788 3739
Fragments arranged by size:
3739 1788 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 2441 2710 1052
Size: 1389 269 4421
Fragments arranged by size:
4421 1389 269
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 3437 4982 1132
Size: 97 2208 1545 2229
Fragments arranged by size:
2229 2208 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2614 2995 1108
Size: 1506 381 4192
Fragments arranged by size:
4192 1506 381
PfoI T'CCnGG_A
Cuts at: 2490 2535 2490
Size: 45 6034
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 4148 4180 5661 5693 2873
Size: 32 1243 32 1481 32 3259
Fragments arranged by size:
3259 1481 1243 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspXI vC'TCGA_Gb
Cuts at: 3230 3230
Size: 6079
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 6047
PstI C_TGCA'G
Cuts at: 830 2022 3012 3081 3087 3431 830
Size: 1192 990 69 6 344 3478
Fragments arranged by size:
3478 1192 990 344 69 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2071 2129 3019 3082 3124 2071
Size: 58 890 63 42 5026
Fragments arranged by size:
5026 890 63 58 42
SacI G_AGCT'C
Cuts at: 2027 2490 2658 2976 3135 2027
Size: 463 168 318 159 4971
Fragments arranged by size:
4971 463 318 168 159
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SapI GCTCTTCn'nnn_
Cuts at: 2478 2700 2478
Size: 222 5857
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SexAI A'CCwGG_T
Cuts at: 2760 2760
Size: 6079
SfcI C'TryA_G
Cuts at: 826 1045 2018 2458 3008 3077 3083 3427 4562
Size: 219 973 440 550 69 6 344 1135
Cuts at: 4562 5243 5434 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 973 681 550 440 344 219
191 69 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 3230 4183 5084 5346 820
Size: 197 926 636 651 953 901 262 1553
Fragments arranged by size:
1553 953 926 901 651 636 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 2994 3376 1517
Size: 1477 382 4220
Fragments arranged by size:
4220 1477 382
StyI C'CwwG_G
Cuts at: 513 1065 2209 2233 2853 2989 3518 513
Size: 552 1144 24 620 136 529 3074
Fragments arranged by size:
3074 1144 620 552 529 136 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3165 4325 6037
Size: 274 80 33 51 1443 1188 1160 1712
Cuts at: 6037 96
Size: 138
Fragments arranged by size:
1712 1443 1188 1160 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2133 2165 6039 6071 2133
Size: 32 3874 32 2141
Fragments arranged by size:
3874 2141 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2395 2560 2395
Size: 165 5914
XhoI C'TCGA_G
Cuts at: 1943 3230 1943
Size: 1287 4792
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI RsrII SacII
SanDI SfiI SfoI SrfI SwaI