Restriction map for
pFN21AB6684
(Circular) (Six-base) MAPSORT of: pFN21AB6684.seq Check: 6359 from: 1 to: 6073
pFN21AB6684 6073 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2378 2388 2982 3416 1937
Size: 441 10 594 434 4594
Fragments arranged by size:
4594 594 441 434 10
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3377 4136 5520 5788 1632
Size: 1745 759 1384 268 1917
Fragments arranged by size:
1917 1745 1384 759 268
AfeI AGC'GCT
Cuts at: 2615 2615
Size: 6073
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 2695 4972 5949 1203
Size: 1492 2277 977 1327
Fragments arranged by size:
2277 1492 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 3816 5388 3816
Size: 1572 4501
ApaI G_GGCC'C
Cuts at: 2512 2512
Size: 6073
ApaLI G'TGCA_C
Cuts at: 2795 4130 5286 2795
Size: 1335 1156 3582
Fragments arranged by size:
3582 1335 1156
ApoI r'AATT_y
Cuts at: 1493 2821 3197 3382 3608 3760 5869 1493
Size: 1328 376 185 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 376 226 185 152
AseI AT'TA_AT
Cuts at: 160 1032 4627 160
Size: 872 3595 1606
Fragments arranged by size:
3595 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2426 3398 1870
Size: 73 483 972 4545
Fragments arranged by size:
4545 972 483 73
BamHI G'GATC_C
Cuts at: 3403 3770 3403
Size: 367 5706
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 3394 4849 618
Size: 325 217 627 354 1253 1455 1842
Fragments arranged by size:
1842 1455 1253 627 354 325 217
BanII G_rGCy'C
Cuts at: 1391 2512 3392 1391
Size: 1121 880 4072
Fragments arranged by size:
4072 1121 880
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3204 928
Size: 971 1305 3797
Fragments arranged by size:
3797 1305 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4264 4298 1903
Size: 34 50 34 2243 34 3678
Fragments arranged by size:
3678 2243 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 2779 1548
Size: 1231 4842
BfrBI ATG'CAT
Cuts at: 3019 3019
Size: 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4684 136
Size: 107 122 71 631 605 3012 1525
Fragments arranged by size:
3012 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2430 6068 2430
Size: 3638 2435
BlpI GC'TnA_GC
Cuts at: 2060 3490 2060
Size: 1430 4643
Bme1580I G_kGCm'C
Cuts at: 1790 2512 2799 4134 5290 1790
Size: 722 287 1335 1156 2573
Fragments arranged by size:
2573 1335 1156 722 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3804 4762 442
Size: 416 53 83 399 24 2387 958 1753
Fragments arranged by size:
2387 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2651
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2646 2837 4733 1239
Size: 1407 191 1896 2579
Fragments arranged by size:
2579 1896 1407 191
Bpu10I CC'TnA_GC
Cuts at: 3089 3129 3089
Size: 40 6033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4198 5063 5361 4198
Size: 865 298 4910
Fragments arranged by size:
4910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4736 882
Size: 617 3237 2219
Fragments arranged by size:
3237 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3864 493
Size: 711 2660 2702
Fragments arranged by size:
2702 2660 711
BsaBI GATnn'nnATC
Cuts at: 6067 6067
Size: 6073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3880 4262 4897 275
Size: 53 83 186 936 2347 382 635 1451
Fragments arranged by size:
2347 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3237 4506 4904 5178 5325 5736 3237
Size: 1269 398 274 147 411 3574
Fragments arranged by size:
3574 1269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5848
Fragments arranged by size:
5848 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 1186
Size: 970 5103
BseYI C'CCAG_C
Cuts at: 2461 2551 5276 5772 2461
Size: 90 2725 496 2762
Fragments arranged by size:
2762 2725 496 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3247 1163
Size: 573 1511 3989
Fragments arranged by size:
3989 1511 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2799 3007 4134 4219 5290 2799
Size: 208 1127 85 1071 3582
Fragments arranged by size:
3582 1127 1071 208 85
BsmI GAATG_Cn'
Cuts at: 2347 3584 3677 2347
Size: 1237 93 4743
Fragments arranged by size:
4743 1237 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2512 2799 3007 3392 4134 4219 5290
Size: 399 722 287 208 385 742 85 1071
Cuts at: 5290 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 722 399 385 287 208
85
BspEI T'CCGG_A
Cuts at: 5736 5736
Size: 6073
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 3428 844
Size: 1903 681 3489
Fragments arranged by size:
3489 1903 681
BsrBI CCG'CTC
Cuts at: 1394 1823 2962 3960 1394
Size: 429 1139 998 3507
Fragments arranged by size:
3507 1139 998 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4568 4742 65
Size: 1183 3320 174 1396
Fragments arranged by size:
3320 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4717 4904 1700
Size: 110 2907 187 2869
Fragments arranged by size:
2907 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 2593 3029 4133 5145 2593
Size: 436 1104 1012 3521
Fragments arranged by size:
3521 1104 1012 436
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2342 794
Size: 1548 4525
BstBI TT'CG_AA
Cuts at: 2180 4877 2180
Size: 2697 3376
BstEII G'GTnAC_C
Cuts at: 1184 3079 1184
Size: 1895 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 2019 2231 2492 2019
Size: 212 261 5600
Fragments arranged by size:
5600 261 212
BstYI r'GATC_y
Cuts at: 1972 2430 3403 3770 4155 4172 5624 5710 5722
Size: 458 973 367 385 17 1452 86 12
Cuts at: 5722 6068 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 973 458 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2269 2358 513
Size: 552 1204 89 4228
Fragments arranged by size:
4228 1204 552 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5334
Fragments arranged by size:
5334 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 3264 3589 4383 4403 2221
Size: 1043 325 794 20 3891
Fragments arranged by size:
3891 1043 794 325 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2708 5080 809
Size: 507 1392 2372 1802
Fragments arranged by size:
2372 1802 1392 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3520 4409 6038
Size: 54 1425 50 275 18 1690 889 1629
Cuts at: 6038 8
Size: 43
Fragments arranged by size:
1690 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 2604 2642 3112 4003 1353
Size: 1190 61 38 470 891 3423
Fragments arranged by size:
3423 1190 891 470 61 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4660 5034 5180 387
Size: 1064 138 3071 374 146 1280
Fragments arranged by size:
3071 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2646 2837 3377 4136 4733 5520 5788
Size: 393 1014 191 540 759 597 787 268
Cuts at: 5788 1239
Size: 1524
Fragments arranged by size:
1524 1014 787 759 597 540 393 268
191
EcoNI CCTnn'n_nnAGG
Cuts at: 2917 2917
Size: 6073
EcoO109I rG'GnC_Cy
Cuts at: 2015 2044 2508 2015
Size: 29 464 5580
Fragments arranged by size:
5580 464 29
EcoRI G'AATT_C
Cuts at: 2821 3382 3760 2821
Size: 561 378 5134
Fragments arranged by size:
5134 561 378
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 2127 2617 2814 5220 1678
Size: 449 490 197 2406 2531
Fragments arranged by size:
2531 2406 490 449 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2764 2796 4714 4746 4788
Size: 32 135 32 1192 32 1918 32 42
Cuts at: 4788 4820 1373
Size: 32 2626
Fragments arranged by size:
2626 1918 1192 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2713 3417 3663 669
Size: 988 281 775 704 246 3079
Fragments arranged by size:
3079 988 775 704 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6073
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2047 2451 2671 3240 5187 5371 1305
Size: 742 404 220 569 1947 184 2007
Fragments arranged by size:
2007 1947 742 569 404 220 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4251
Fragments arranged by size:
4251 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4031 4390 4549 518
Size: 648 2865 359 159 2042
Fragments arranged by size:
2865 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2399 2551 3489 4166 5314 5559 1606
Size: 793 152 938 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 793 677 245 152
NcoI C'CATG_G
Cuts at: 513 1065 2269 513
Size: 552 1204 4317
Fragments arranged by size:
4317 1204 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NsiI A_TGCA'T
Cuts at: 3021 3021
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 2699 3019 3431 4976 1132
Size: 97 1470 320 412 1545 2229
Fragments arranged by size:
2229 1545 1470 412 320 97
PasI CC'CwG_GG
Cuts at: 1348 2019 1348
Size: 671 5402
PciI A'CATG_T
Cuts at: 2695 4972 2695
Size: 2277 3796
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6073
PfoI T'CCnGG_A
Cuts at: 2300 2300
Size: 6073
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2015 2015
Size: 6073
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6073
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PspOMI G'GGCC_C
Cuts at: 2508 2508
Size: 6073
PstI C_TGCA'G
Cuts at: 830 3425 830
Size: 2595 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
PvuII CAG'CTG
Cuts at: 2399 2551 2399
Size: 152 5921
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SapI GCTCTTCn'nnn_
Cuts at: 2543 2604 2543
Size: 61 6012
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 4321 6033 4321
Size: 1712 4361
SfcI C'TryA_G
Cuts at: 826 1045 2379 3421 4556 5237 5428 826
Size: 219 1334 1042 1135 681 191 1471
Fragments arranged by size:
1471 1334 1135 1042 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 3400 1872
Size: 1528 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 4177 5078 5340 820
Size: 197 926 483 1751 901 262 1553
Fragments arranged by size:
1751 1553 926 901 483 262 197
SnaBI TAC'GTA
Cuts at: 493 3864 493
Size: 3371 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3019 3431 1132
Size: 1887 412 3774
Fragments arranged by size:
3774 1887 412
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 2568 2864 1517
Size: 1051 296 4726
Fragments arranged by size:
4726 1051 296
StyI C'CwwG_G
Cuts at: 513 1065 2269 2918 2967 3512 513
Size: 552 1204 649 49 545 3074
Fragments arranged by size:
3074 1204 649 552 545 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2453 4100 4117 4270 4455 4900
Size: 1255 446 427 1647 17 153 185 445
Cuts at: 4900 325
Size: 1498
Fragments arranged by size:
1647 1498 1255 446 445 427 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2589 4319 6031
Size: 274 80 33 51 1443 612 1730 1712
Cuts at: 6031 96
Size: 138
Fragments arranged by size:
1730 1712 1443 612 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6033 6065 6033
Size: 32 6041
Tth111I GACn'n_nGTC
Cuts at: 1316 2880 1316
Size: 1564 4509
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2729 2729
Size: 6073
XhoI C'TCGA_G
Cuts at: 1943 2426 1943
Size: 483 5590
XmaI C'CCGG_G
Cuts at: 1870 3398 1870
Size: 1528 4545
XmnI GAAnn'nnTTC
Cuts at: 4202 4202
Size: 6073
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI