Restriction map for
pFN21AB6681
(Circular) (Six-base) MAPSORT of: pFN21AB6681.seq Check: 8489 from: 1 to: 5986
pFN21AB6681 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2758 2764 2785 2758
Size: 6 21 5959
Fragments arranged by size:
5959 21 6
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 2678 2708 3108 4049 5433 5701 1632
Size: 845 201 30 400 941 1384 268 1917
Fragments arranged by size:
1917 1384 941 845 400 268 201 30
AfeI AGC'GCT
Cuts at: 2876 2876
Size: 5986
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 3143 3242 1166
Size: 1977 99 3910
Fragments arranged by size:
3910 1977 99
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 2201 2614 3729 5301 2201
Size: 413 1115 1572 2886
Fragments arranged by size:
2886 1572 1115 413
ApaI G_GGCC'C
Cuts at: 2791 2992 2791
Size: 201 5785
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2381 3295 3521 3673 5782 1493
Size: 888 914 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 888 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2847 2991 2997 3311 1870
Size: 73 904 144 6 314 4545
Fragments arranged by size:
4545 904 314 144 73 6
BamHI G'GATC_C
Cuts at: 2568 3316 3683 2568
Size: 748 367 4871
Fragments arranged by size:
4871 748 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3307 4762 618
Size: 325 217 627 1520 1455 1842
Fragments arranged by size:
1842 1520 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2791 2992 2998 3305 1391
Size: 1400 201 6 307 4072
Fragments arranged by size:
4072 1400 307 201 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 928
Size: 971 383 4632
Fragments arranged by size:
4632 971 383
BbvCI CC'TCA_GC
Cuts at: 2970 2970
Size: 5986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2339 2987 3026
Size: 107 122 71 631 605 667 648 39
Cuts at: 3026 4597 136
Size: 1571 1525
Fragments arranged by size:
1571 1525 667 648 631 605 122 107
71 39
BglII A'GATC_T
Cuts at: 2361 5981 2361
Size: 3620 2366
BlpI GC'TnA_GC
Cuts at: 3009 3060 3403 3009
Size: 51 343 5592
Fragments arranged by size:
5592 343 51
Bme1580I G_kGCm'C
Cuts at: 1790 2791 2992 4047 5203 1790
Size: 1001 201 1055 1156 2573
Fragments arranged by size:
2573 1156 1055 1001 201
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 3195 3717
Size: 416 53 83 399 24 643 1135 522
Cuts at: 3717 4675 442
Size: 958 1753
Fragments arranged by size:
1753 1135 958 643 522 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2145 2145
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2327 2861 2906 4646 1239
Size: 861 227 534 45 1740 2579
Fragments arranged by size:
2579 1740 861 534 227 45
Bpu10I CC'TnA_GC
Cuts at: 2970 2970
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2499 4111 4976 5274 2499
Size: 1612 865 298 3211
Fragments arranged by size:
3211 1612 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2047 4649 882
Size: 617 548 2602 2219
Fragments arranged by size:
2602 2219 617 548
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2073 2103 1178
Size: 30 165 30 670 30 5061
Fragments arranged by size:
5061 670 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2401 2768 3116 1186
Size: 1186 29 367 348 4056
Fragments arranged by size:
4056 1186 367 348 29
BseYI C'CCAG_C
Cuts at: 2564 2790 5189 5685 2564
Size: 226 2399 496 2865
Fragments arranged by size:
2865 2399 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2786 2792 2825 1163
Size: 573 1050 6 33 4324
Fragments arranged by size:
4324 1050 573 33 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2253 3436 4197 5225 1540
Size: 275 186 252 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 2701 4047 4132 5203 2701
Size: 1346 85 1071 3484
Fragments arranged by size:
3484 1346 1071 85
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
BsmBI CGTCTCn'nnnn_
Cuts at: 2303 2303
Size: 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2701 2791 2992 2998 3305 4047 4132
Size: 399 911 90 201 6 307 742 85
Cuts at: 4132 5203 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 911 742 399 307 201 90
85 6
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2758 2764 2785 3341 844
Size: 1914 6 21 556 3489
Fragments arranged by size:
3489 1914 556 21 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2785 3873 1394
Size: 429 962 1088 3507
Fragments arranged by size:
3507 1088 962 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2205 2298 4481 4655 65
Size: 1183 957 93 2183 174 1396
Fragments arranged by size:
2183 1396 1183 957 174 93
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2764 794
Size: 1970 4016
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2115 2124 2361 2568 3316 3683 4068 4085
Size: 143 9 237 207 748 367 385 17
Cuts at: 4085 5537 5623 5635 5981 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 748 385 367 346 237 207
143 86 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2034 2179 3165 513
Size: 552 969 145 986 3334
Fragments arranged by size:
3334 986 969 552 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2808 3163 3502 4296 4316 2808
Size: 355 339 794 20 4478
Fragments arranged by size:
4478 794 355 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2681 4993 809
Size: 507 1365 2312 1802
Fragments arranged by size:
2312 1802 1365 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2631 2730
Size: 54 1425 50 275 18 420 381 99
Cuts at: 2730 3018 3433 4322 5951 8
Size: 288 415 889 1629 43
Fragments arranged by size:
1629 1425 889 420 415 381 288 275
99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2250 3433 1537
Size: 275 438 1183 4090
Fragments arranged by size:
4090 1183 438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3916 1353
Size: 2563 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2945 4573 4947 5093 387
Size: 1064 138 1356 1628 374 146 1280
Fragments arranged by size:
1628 1356 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2327 2477 2678 2708 2861 2906
Size: 393 468 227 150 201 30 153 45
Cuts at: 2906 3108 4049 4646 5433 5701 1239
Size: 202 941 597 787 268 1524
Fragments arranged by size:
1524 941 787 597 468 393 268 227
202 201 153 150 45 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2897 2897
Size: 5986
EcoO109I rG'GnC_Cy
Cuts at: 2185 2988 2185
Size: 803 5183
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 2878 2893 5133 1678
Size: 1200 15 2240 2531
Fragments arranged by size:
2531 2240 1200 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 3097 3129 4627
Size: 32 135 32 501 32 992 32 1498
Cuts at: 4627 4659 4701 4733 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1498 992 501 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 2686 3330 3576 669
Size: 988 281 487 261 644 246 3079
Fragments arranged by size:
3079 988 644 487 281 261 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2134 5100 5284 1305
Size: 829 2966 184 2007
Fragments arranged by size:
2966 2007 829 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2633 2732 3020 10
Size: 54 1425 343 801 99 288 2976
Fragments arranged by size:
2976 1425 801 343 288 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2438 3143 3242 3944 4303 4462
Size: 648 1185 87 705 99 702 359 159
Cuts at: 4462 518
Size: 2042
Fragments arranged by size:
2042 1185 705 702 648 359 159 99
87
MspA1I CmG'CkG
Cuts at: 1606 2761 2864 3043 3064 3153 3402 4079 5227
Size: 1155 103 179 21 89 249 677 1148
Cuts at: 5227 5472 1606
Size: 245 2120
Fragments arranged by size:
2120 1155 1148 677 249 245 179 103
89 21
NcoI C'CATG_G
Cuts at: 513 1065 2034 2179 3165 513
Size: 552 969 145 986 3334
Fragments arranged by size:
3334 986 969 552 145
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 2250 3433 2250
Size: 1183 4803
NspI r_CATG'y
Cuts at: 1132 1229 3073 3344 4889 1132
Size: 97 1844 271 1545 2229
Fragments arranged by size:
2229 1844 1545 271 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2797 1108
Size: 1689 4297
PfoI T'CCnGG_A
Cuts at: 2571 2571
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2185 2185
Size: 5986
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PspOMI G'GGCC_C
Cuts at: 2787 2988 2787
Size: 201 5785
PstI C_TGCA'G
Cuts at: 830 3338 830
Size: 2508 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 3043 3064 3043
Size: 21 5965
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SanDI GG'GwC_CC
Cuts at: 2185 2185
Size: 5986
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 3195 4234 5946 3195
Size: 1039 1712 3235
Fragments arranged by size:
3235 1712 1039
SexAI A'CCwGG_T
Cuts at: 2418 2418
Size: 5986
SfcI C'TryA_G
Cuts at: 826 1045 3334 4469 5150 5341 826
Size: 219 2289 1135 681 191 1471
Fragments arranged by size:
2289 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2339 2339
Size: 5986
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 2849 3313 1872
Size: 977 464 4545
Fragments arranged by size:
4545 977 464
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2514 4090 4991 5253 820
Size: 197 926 571 1576 901 262 1553
Fragments arranged by size:
1576 1553 926 901 571 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 2975 1517
Size: 1458 4528
StyI C'CwwG_G
Cuts at: 513 1065 2034 2179 2634 3165 3425 513
Size: 552 969 145 455 531 260 3074
Fragments arranged by size:
3074 969 552 531 455 260 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4013 4030 4183 4368 4813 325
Size: 1255 2433 17 153 185 445 1498
Fragments arranged by size:
2433 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3193 4232 5944
Size: 274 80 33 51 1443 1216 1039 1712
Cuts at: 5944 96
Size: 138
Fragments arranged by size:
1712 1443 1216 1039 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 2420 1316
Size: 1104 4882
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 2847 3311 1870
Size: 977 464 4545
Fragments arranged by size:
4545 977 464
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SapI SfoI SrfI
SwaI XcmI