Restriction map for
pFN21AB6680
(Circular) (Six-base) MAPSORT of: pFN21AB6680.seq Check: 9829 from: 1 to: 6073
pFN21AB6680 6073 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3416 1937
Size: 1479 4594
Acc65I G'GTAC_C
Cuts at: 2424 3394 2424
Size: 970 5103
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2560 2744 2881 3267 4136 5520 5788
Size: 516 412 184 137 386 869 1384 268
Cuts at: 5788 1632
Size: 1917
Fragments arranged by size:
1917 1384 869 516 412 386 268 184
137
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 4972 5949 1203
Size: 3769 977 1327
Fragments arranged by size:
3769 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 2998 3816 5388 2998
Size: 818 1572 3683
Fragments arranged by size:
3683 1572 818
ApaLI G'TGCA_C
Cuts at: 4130 5286 4130
Size: 1156 4917
ApoI r'AATT_y
Cuts at: 1493 3382 3608 3760 5869 1493
Size: 1889 226 152 2109 1697
Fragments arranged by size:
2109 1889 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4627 160
Size: 872 3595 1606
Fragments arranged by size:
3595 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2529 3233 3398 1870
Size: 73 115 471 704 165 4545
Fragments arranged by size:
4545 704 471 165 115 73
BamHI G'GATC_C
Cuts at: 3403 3770 3403
Size: 367 5706
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2424 3394 4849 618
Size: 325 217 627 637 970 1455 1842
Fragments arranged by size:
1842 1455 970 637 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2493 3105 3138 3392 1391
Size: 1102 612 33 254 4072
Fragments arranged by size:
4072 1102 612 254 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3214 3248 4264 4298 1903
Size: 34 1277 34 1016 34 3678
Fragments arranged by size:
3678 1277 1016 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2561 3254 4684
Size: 107 122 71 631 605 889 693 1430
Cuts at: 4684 136
Size: 1525
Fragments arranged by size:
1525 1430 889 693 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6068 6068
Size: 6073
BlpI GC'TnA_GC
Cuts at: 3023 3490 3023
Size: 467 5606
Bme1580I G_kGCm'C
Cuts at: 1790 2380 4134 5290 1790
Size: 590 1754 1156 2573
Fragments arranged by size:
2573 1754 1156 590
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3804 4762 442
Size: 416 53 83 399 24 2387 958 1753
Fragments arranged by size:
2387 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2717 1056
Size: 1661 4412
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 2423 2561 2702 3020 3062 3137 3221
Size: 1163 21 138 141 318 42 75 84
Cuts at: 3221 4733 1239
Size: 1512 2579
Fragments arranged by size:
2579 1512 1163 318 141 138 84 75
42 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 4198 5063 5361 2567
Size: 1631 865 298 3279
Fragments arranged by size:
3279 1631 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2306 4736 882
Size: 617 807 2430 2219
Fragments arranged by size:
2430 2219 807 617
BsaAI yAC'GTr
Cuts at: 493 1204 2375 3864 493
Size: 711 1171 1489 2702
Fragments arranged by size:
2702 1489 1171 711
BsaBI GATnn'nnATC
Cuts at: 2690 6067 2690
Size: 3377 2696
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3880 4262 4897 275
Size: 53 83 186 936 2347 382 635 1451
Fragments arranged by size:
2347 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4506 4904 5178 5325 5736 4506
Size: 398 274 147 411 4843
Fragments arranged by size:
4843 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2241 2271 1178
Size: 30 165 30 838 30 4980
Fragments arranged by size:
4980 838 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2190 2294 2399 2723 2756 2924 1186
Size: 1004 104 105 324 33 168 4335
Fragments arranged by size:
4335 1004 324 168 105 104 33
BseYI C'CCAG_C
Cuts at: 2078 2440 3018 3035 3123 5276 5772 2078
Size: 362 578 17 88 2153 496 2379
Fragments arranged by size:
2379 2153 578 496 362 88 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5500
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 3138 4134 4219 5290 2493
Size: 645 996 85 1071 3276
Fragments arranged by size:
3276 1071 996 645 85
BsmI GAATG_Cn'
Cuts at: 2459 3584 3677 2459
Size: 1125 93 4855
Fragments arranged by size:
4855 1125 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2974 2974
Size: 6073
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2380 2493 3105 3138 3392 4134 4219
Size: 399 590 113 612 33 254 742 85
Cuts at: 4219 5290 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 612 590 399 254 113
85 33
BspEI T'CCGG_A
Cuts at: 5736 5736
Size: 6073
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3428 844
Size: 2584 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3960 1394
Size: 429 2137 3507
Fragments arranged by size:
3507 2137 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2320 4568 4742 65
Size: 1183 1072 2248 174 1396
Fragments arranged by size:
2248 1396 1183 1072 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2771 4717 4904 1700
Size: 110 961 1946 187 2869
Fragments arranged by size:
2869 1946 961 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6073
BstBI TT'CG_AA
Cuts at: 4877 4877
Size: 6073
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6073
BstYI r'GATC_y
Cuts at: 1972 2412 3093 3403 3770 4155 4172 5624 5710
Size: 440 681 310 367 385 17 1452 86
Cuts at: 5710 5722 6068 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 681 440 385 367 346 310
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2856 513
Size: 552 1791 3730
Fragments arranged by size:
3730 1791 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2307 487
Size: 683 56 1081 4253
Fragments arranged by size:
4253 1081 683 56
BtsI GCAGTG_nn'
Cuts at: 3589 4383 4403 3589
Size: 794 20 5259
Fragments arranged by size:
5259 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DraIII CAC_nnn'GTG
Cuts at: 2807 2807
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2508 2667 2769
Size: 54 1425 50 275 18 678 159 102
Cuts at: 2769 2773 3054 3520 4409 6038 8
Size: 4 281 466 889 1629 43
Fragments arranged by size:
1629 1425 889 678 466 281 275 159
102 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2167 2481 2733 3286 3321 4003 1353
Size: 814 314 252 553 35 682 3423
Fragments arranged by size:
3423 814 682 553 314 252 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2876 4660 5034 5180 387
Size: 1064 138 1287 1784 374 146 1280
Fragments arranged by size:
1784 1287 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2491 3136 2491
Size: 645 5428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2402 2423 2560 2561 2702 2744
Size: 393 516 254 21 137 1 141 42
Cuts at: 2744 2881 3020 3062 3137 3221 3267 4136 4733
Size: 137 139 42 75 84 46 869 597
Cuts at: 4733 5520 5788 1239
Size: 787 268 1524
Fragments arranged by size:
1524 869 787 597 516 393 268 254
141 139 137 137 84 75 46 42
42 21 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2384 2609 2705 3233 2366
Size: 18 225 96 528 5206
Fragments arranged by size:
5206 528 225 96 18
EcoO109I rG'GnC_Cy
Cuts at: 2698 2698
Size: 6073
EcoRI G'AATT_C
Cuts at: 3382 3760 3382
Size: 378 5695
FseI GG_CCGG'CC
Cuts at: 2775 2775
Size: 6073
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 5220 1678
Size: 3542 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2999 3031 3139 3171 4714
Size: 32 135 32 1427 32 108 32 1543
Cuts at: 4714 4746 4788 4820 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1543 1427 135 108 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3417 3663 669
Size: 988 281 1479 246 3079
Fragments arranged by size:
3079 1479 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6073
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 2428 3398 2428
Size: 970 5103
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2958 5187 5371 1305
Size: 1653 2229 184 2007
Fragments arranged by size:
2229 2007 1653 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2510 2669 10
Size: 54 1425 343 678 159 3414
Fragments arranged by size:
3414 1425 678 343 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2942 3318 4031 4390 4549 518
Size: 648 1776 376 713 359 159 2042
Fragments arranged by size:
2042 1776 713 648 376 359 159
MspA1I CmG'CkG
Cuts at: 1606 2767 3085 3127 3489 4166 5314 5559 1606
Size: 1161 318 42 362 677 1148 245 2120
Fragments arranged by size:
2120 1161 1148 677 362 318 245 42
NaeI GCC'GGC
Cuts at: 2773 2773
Size: 6073
NcoI C'CATG_G
Cuts at: 513 1065 2856 513
Size: 552 1791 3730
Fragments arranged by size:
3730 1791 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NgoMIV G'CCGG_C
Cuts at: 2771 2771
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 2713 1052
Size: 1661 4412
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 3431 4976 1132
Size: 97 2202 1545 2229
Fragments arranged by size:
2229 2202 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6073
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6073
PfoI T'CCnGG_A
Cuts at: 2493 2538 2649 2493
Size: 45 111 5917
Fragments arranged by size:
5917 111 45
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2908 4142 4174 5655 5687 2876
Size: 32 1234 32 1481 32 3262
Fragments arranged by size:
3262 1481 1234 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6073
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PspXI vC'TCGA_Gb
Cuts at: 3233 3233
Size: 6073
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 6041
PstI C_TGCA'G
Cuts at: 830 3015 3090 3425 830
Size: 2185 75 335 3478
Fragments arranged by size:
3478 2185 335 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
PvuII CAG'CTG
Cuts at: 2767 3085 3127 2767
Size: 318 42 5713
Fragments arranged by size:
5713 318 42
SacI G_AGCT'C
Cuts at: 2493 3138 2493
Size: 645 5428
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SapI GCTCTTCn'nnn_
Cuts at: 2481 2481
Size: 6073
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 4321 6033 4321
Size: 1712 4361
SfcI C'TryA_G
Cuts at: 826 1045 2461 3011 3086 3421 4556 5237 5428
Size: 219 1416 550 75 335 1135 681 191
Cuts at: 5428 826
Size: 1471
Fragments arranged by size:
1471 1416 1135 681 550 335 219 191
75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 2531 3400 1872
Size: 659 869 4545
Fragments arranged by size:
4545 869 659
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2582 3233 4177 5078 5340 820
Size: 197 926 639 651 944 901 262 1553
Fragments arranged by size:
1553 944 926 901 651 639 262 197
SnaBI TAC'GTA
Cuts at: 493 3864 493
Size: 3371 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 2888 1517
Size: 1371 4702
StyI C'CwwG_G
Cuts at: 513 1065 2856 3512 513
Size: 552 1791 656 3074
Fragments arranged by size:
3074 1791 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4100 4117 4270 4455 4900 325
Size: 1255 2520 17 153 185 445 1498
Fragments arranged by size:
2520 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3168 4319 6031
Size: 274 80 33 51 1443 1191 1151 1712
Cuts at: 6031 96
Size: 138
Fragments arranged by size:
1712 1443 1191 1151 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6033 6065 6033
Size: 32 6041
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2677 3104 2398
Size: 279 427 5367
Fragments arranged by size:
5367 427 279
XhoI C'TCGA_G
Cuts at: 1943 3233 1943
Size: 1290 4783
XmaI C'CCGG_G
Cuts at: 1870 2529 3398 1870
Size: 659 869 4545
Fragments arranged by size:
4545 869 659
XmnI GAAnn'nnTTC
Cuts at: 2753 4202 2753
Size: 1449 4624
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspOMI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI