Restriction map for
pFN21AB6677
(Circular) (Six-base) MAPSORT of: pFN21AB6677.seq Check: 3044 from: 1 to: 5986
pFN21AB6677 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 2683 3307 2683
Size: 624 5362
AclI AA'CG_TT
Cuts at: 1563 3285 4113 4486 1563
Size: 1722 828 373 3063
Fragments arranged by size:
3063 1722 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 2682 4049 5433 5701 1632
Size: 585 465 1367 1384 268 1917
Fragments arranged by size:
1917 1384 1367 585 465 268
AflII C'TTAA_G
Cuts at: 820 1017 2897 820
Size: 197 1880 3909
Fragments arranged by size:
3909 1880 197
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 3729 5301 3729
Size: 1572 4414
ApaLI G'TGCA_C
Cuts at: 2812 4043 5199 2812
Size: 1231 1156 3599
Fragments arranged by size:
3599 1231 1156
ApoI r'AATT_y
Cuts at: 1493 2741 3085 3128 3295 3521 3673 5782 1493
Size: 1248 344 43 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 1248 344 226 167 152 43
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 3311 1870
Size: 73 1368 4545
Fragments arranged by size:
4545 1368 73
BamHI G'GATC_C
Cuts at: 2634 3316 3683 2634
Size: 682 367 4937
Fragments arranged by size:
4937 682 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 3307 4762 618
Size: 325 217 627 896 624 1455 1842
Fragments arranged by size:
1842 1455 896 627 624 325 217
BanII G_rGCy'C
Cuts at: 1391 2139 3305 1391
Size: 748 1166 4072
Fragments arranged by size:
4072 1166 748
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 2449 1548
Size: 901 5085
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 2138 3403 2138
Size: 1265 4721
Bme1580I G_kGCm'C
Cuts at: 1790 2816 4047 5203 1790
Size: 1026 1231 1156 2573
Fragments arranged by size:
2573 1231 1156 1026
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2593 4646 1239
Size: 1354 2053 2579
Fragments arranged by size:
2579 2053 1354
Bpu10I CC'TnA_GC
Cuts at: 2309 2309
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2893 4111 4976 5274 2893
Size: 1218 865 298 3605
Fragments arranged by size:
3605 1218 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2373 4649 882
Size: 617 874 2276 2219
Fragments arranged by size:
2276 2219 874 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3258 4419 4817 5091 5238 5649 3258
Size: 1161 398 274 147 411 3595
Fragments arranged by size:
3595 1161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5986
BseYI C'CCAG_C
Cuts at: 2183 2432 5189 5685 2183
Size: 249 2757 496 2484
Fragments arranged by size:
2757 2484 496 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2816 4047 4132 5203 2139
Size: 677 1231 85 1071 2922
Fragments arranged by size:
2922 1231 1071 677 85
BsmI GAATG_Cn'
Cuts at: 2302 3497 3590 2302
Size: 1195 93 4698
Fragments arranged by size:
4698 1195 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2816 3305 4047 4132 5203 1391
Size: 399 349 677 489 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 677 489 399 349 85
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 2835 3875 5605 2835
Size: 1040 1730 3216
Fragments arranged by size:
3216 1730 1040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3341 844
Size: 2497 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2194 4481 4655 65
Size: 1183 946 2287 174 1396
Fragments arranged by size:
2287 1396 1183 946 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 2149 1184
Size: 965 5021
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2173
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2634 3316 3683 4068 4085 5537 5623 5635
Size: 662 682 367 385 17 1452 86 12
Cuts at: 5635 5981 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 682 662 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2021 2021
Size: 5986
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2814 3502 4296 4316 2814
Size: 688 794 20 4484
Fragments arranged by size:
4484 794 688 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3133 3290 3133
Size: 157 5829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2652 3433 4322
Size: 54 1425 50 275 18 822 781 889
Cuts at: 4322 5951 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 822 781 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2786 2973 3916 1353
Size: 1433 187 943 3423
Fragments arranged by size:
3423 1433 943 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2137 2137
Size: 5986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2593 2682 4049 4646 5433 5701
Size: 393 585 376 89 1367 597 787 268
Cuts at: 5701 1239
Size: 1524
Fragments arranged by size:
1524 1367 787 597 585 393 376 268
89
EcoRI G'AATT_C
Cuts at: 3085 3295 3673 3085
Size: 210 378 5398
Fragments arranged by size:
5398 378 210
EcoRV GAT'ATC
Cuts at: 2262 2262
Size: 5986
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 2440 5133 1678
Size: 762 2693 2531
Fragments arranged by size:
2693 2531 762
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2043 2075 2350 2370 2382
Size: 32 135 32 471 32 275 20 12
Cuts at: 2382 2402 4627 4659 4701 4733 1373
Size: 20 2225 32 42 32 2626
Fragments arranged by size:
2626 2225 471 275 135 42 32 32
32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 3330 3576 669
Size: 988 281 610 782 246 3079
Fragments arranged by size:
3079 988 782 610 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 2687 3311 2687
Size: 624 5362
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2029 5100 5284 1305
Size: 724 3071 184 2007
Fragments arranged by size:
3071 2007 724 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2654 10
Size: 54 1425 343 822 3342
Fragments arranged by size:
3342 1425 822 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2106 2834 3944 4303 4462 518
Size: 648 940 728 1110 359 159 2042
Fragments arranged by size:
2042 1110 940 728 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3402 4079 5227 5472 1606
Size: 1796 677 1148 245 2120
Fragments arranged by size:
2120 1796 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 3344 4889 1132
Size: 97 2115 1545 2229
Fragments arranged by size:
2229 2115 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5986
PfoI T'CCnGG_A
Cuts at: 2570 2570
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3194 1613
Size: 1581 4405
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 3338 830
Size: 2508 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
SacI G_AGCT'C
Cuts at: 2139 2139
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SapI GCTCTTCn'nnn_
Cuts at: 2786 2786
Size: 5986
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 3334 4469 5150 5341 826
Size: 219 2289 1135 681 191 1471
Fragments arranged by size:
2289 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2872 2897 4090 4991 5253 820
Size: 197 926 929 25 1193 901 262 1553
Fragments arranged by size:
1553 1193 929 926 901 262 197 25
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2004 2374 2805 3425 513
Size: 552 939 370 431 620 3074
Fragments arranged by size:
3074 939 620 552 431 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3184 4013 4030 4183 4368 4813 325
Size: 1255 1604 829 17 153 185 445 1498
Fragments arranged by size:
1604 1498 1255 829 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2267 4232 5944
Size: 274 80 33 51 1443 290 1965 1712
Cuts at: 5944 96
Size: 138
Fragments arranged by size:
1965 1712 1443 290 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI