Restriction map for
pFN21AB6676
(Circular) (Six-base) MAPSORT of: pFN21AB6676.seq Check: 2602 from: 1 to: 6067
pFN21AB6676 6067 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 4130 5514 5782 1632
Size: 747 1751 1384 268 1917
Fragments arranged by size:
1917 1751 1384 747 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3116 1184
Size: 1932 4135
AlwNI CAG_nnn'CTG
Cuts at: 2020 2083 3810 5382 2020
Size: 63 1727 1572 2705
Fragments arranged by size:
2705 1727 1572 63
ApaI G_GGCC'C
Cuts at: 2311 2797 2311
Size: 486 5581
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 2363 3054 3376 3602 3754 5863 1493
Size: 870 691 322 226 152 2109 1697
Fragments arranged by size:
2109 1697 870 691 322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 3392 1870
Size: 73 846 603 4545
Fragments arranged by size:
4545 846 603 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3388 4843 618
Size: 325 217 627 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2311 2343 2797 3309 3386 1391
Size: 670 250 32 454 512 77 4072
Fragments arranged by size:
4072 670 512 454 250 77 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2694 2775 928
Size: 971 795 81 4220
Fragments arranged by size:
4220 971 795 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3182 3216 4258 4292 1903
Size: 34 50 34 1161 34 1042 34 3678
Fragments arranged by size:
3678 1161 1042 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BfrBI ATG'CAT
Cuts at: 2588 2588
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3251 4678 136
Size: 107 122 71 631 605 1579 1427 1525
Fragments arranged by size:
1579 1525 1427 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 2060 3484 2060
Size: 1424 4643
Bme1580I G_kGCm'C
Cuts at: 1790 2311 2797 4128 5284 1790
Size: 521 486 1331 1156 2573
Fragments arranged by size:
2573 1331 1156 521 486
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 2509 3374 4727 1239
Size: 1250 20 865 1353 2579
Fragments arranged by size:
2579 1353 1250 865 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3050 4192 5057 5355 3050
Size: 1142 865 298 3762
Fragments arranged by size:
3762 1142 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4730 882
Size: 617 3231 2219
Fragments arranged by size:
3231 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 2043 6061 2043
Size: 4018 2049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3207 3874 4256 4891
Size: 53 83 186 936 1674 667 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1674 1451 936 667 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2413 2973 4500 4898 5172 5319 5730 2413
Size: 560 1527 398 274 147 411 2750
Fragments arranged by size:
2750 1527 560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5842
Fragments arranged by size:
5842 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3002 1186
Size: 1816 4251
BseYI C'CCAG_C
Cuts at: 5270 5766 5270
Size: 496 5571
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5494
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2283 3095 3309 4128 4213 5284 2061
Size: 222 812 214 819 85 1071 2844
Fragments arranged by size:
2844 1071 819 812 222 214 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
BsmBI CGTCTCn'nnnn_
Cuts at: 2734 2734
Size: 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2283 2311 2343 2797 3095 3309
Size: 399 271 222 28 32 454 298 214
Cuts at: 3309 3386 4128 4213 5284 1391
Size: 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 298 271 222
214 85 77 32 28
BspEI T'CCGG_A
Cuts at: 2413 5730 2413
Size: 3317 2750
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3422 844
Size: 2578 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3008 3954 1394
Size: 429 1185 946 3507
Fragments arranged by size:
3507 1185 946 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2031 96
Size: 1935 4132
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 2763 4127 5139 2763
Size: 1364 1012 3691
Fragments arranged by size:
3691 1364 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 2038 2079 2109 3397 3764 4149 4166 5618
Size: 66 41 30 1288 367 385 17 1452
Cuts at: 5618 5704 5716 6062 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1288 385 367 346 86 66
41 30 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3049 513
Size: 552 1984 3531
Fragments arranged by size:
3531 1984 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 3583 4377 4397 3583
Size: 794 20 5253
Fragments arranged by size:
5253 794 20
ClaI AT'CG_AT
Cuts at: 1554 3192 1554
Size: 1638 4429
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 2983 3371 2983
Size: 388 5679
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032
Size: 54 1425 50 275 18 1684 889 1629
Cuts at: 6032 8
Size: 43
Fragments arranged by size:
1684 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 2499 2646 2732 2800 3997 1353
Size: 978 168 147 86 68 1197 3423
Fragments arranged by size:
3423 1197 978 168 147 86 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3269 4654 5028 5174 387
Size: 1064 138 1680 1385 374 146 1280
Fragments arranged by size:
1680 1385 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2059 3307 2059
Size: 1248 4819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2489 2509 3374 4130 4727 5514
Size: 393 747 110 20 865 756 597 787
Cuts at: 5514 5782 1239
Size: 268 1524
Fragments arranged by size:
1524 865 787 756 747 597 393 268
110 20
EcoNI CCTnn'n_nnAGG
Cuts at: 3299 3299
Size: 6067
EcoO109I rG'GnC_Cy
Cuts at: 2307 2793 2307
Size: 486 5581
EcoRI G'AATT_C
Cuts at: 2363 3054 3376 3754 2363
Size: 691 322 378 4676
Fragments arranged by size:
4676 691 378 322
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 5214 1678
Size: 3536 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 4708 4740 4782
Size: 32 135 32 890 32 2214 32 42
Cuts at: 4782 4814 1373
Size: 32 2626
Fragments arranged by size:
2626 2214 890 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 2137 3144 5181 5365 1305
Size: 699 133 1007 2037 184 2007
Fragments arranged by size:
2037 2007 1007 699 184 133
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3098 4025 4384 4543 518
Size: 648 1932 927 359 159 2042
Fragments arranged by size:
2042 1932 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2729 3340 3483 4160 5308 5553 1606
Size: 1123 611 143 677 1148 245 2120
Fragments arranged by size:
2120 1148 1123 677 611 245 143
NcoI C'CATG_G
Cuts at: 513 1065 3049 513
Size: 552 1984 3531
Fragments arranged by size:
3531 1984 552
NdeI CA'TA_TG
Cuts at: 387 2584 387
Size: 2197 3870
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NsiI A_TGCA'T
Cuts at: 2590 2590
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3103 3245 3425 4970 1132
Size: 97 1874 142 180 1545 2229
Fragments arranged by size:
2229 1874 1545 180 142 97
PasI CC'CwG_GG
Cuts at: 1348 3295 1348
Size: 1947 4120
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspOMI G'GGCC_C
Cuts at: 2307 2793 2307
Size: 486 5581
PstI C_TGCA'G
Cuts at: 830 2409 3205 3419 830
Size: 1579 796 214 3478
Fragments arranged by size:
3478 1579 796 214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2729 3340 2729
Size: 611 5456
SacI G_AGCT'C
Cuts at: 2061 3309 2061
Size: 1248 4819
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SapI GCTCTTCn'nnn_
Cuts at: 2331 2331
Size: 6067
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2405 3201 3211 3236 3415 4550 5231
Size: 219 1360 796 10 25 179 1135 681
Cuts at: 5231 5422 826
Size: 191 1471
Fragments arranged by size:
1471 1360 1135 796 681 219 191 179
25 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3065 4171 5072 5334 820
Size: 197 926 1122 1106 901 262 1553
Fragments arranged by size:
1553 1122 1106 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3103 3245 3425 1132
Size: 1971 142 180 3774
Fragments arranged by size:
3774 1971 180 142
SspI AAT'ATT
Cuts at: 5 52 2542 3991 5
Size: 47 2490 1449 2081
Fragments arranged by size:
2490 2081 1449 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2748 3049 3249 3506 513
Size: 552 1683 301 200 257 3074
Fragments arranged by size:
3074 1683 552 301 257 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 2887 4094 4111 4264 4449 4894
Size: 1255 650 657 1207 17 153 185 445
Cuts at: 4894 325
Size: 1498
Fragments arranged by size:
1498 1255 1207 657 650 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 4313 6025
Size: 274 80 33 51 1443 54 2282 1712
Cuts at: 6025 96
Size: 138
Fragments arranged by size:
2282 1712 1443 274 138 80 54 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 2944 1316
Size: 1628 4439
XbaI T'CTAG_A
Cuts at: 2260 3403 2260
Size: 1143 4924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3292 3292
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 3084 4196 3084
Size: 1112 4955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI