Restriction map for
pFN21AB6673
(Circular) (Six-base) MAPSORT of: pFN21AB6673.seq Check: 4546 from: 1 to: 5986
pFN21AB6673 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2282 2282
Size: 5986
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 2460 3307 2460
Size: 847 5139
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2383 4049 5433 5701 1632
Size: 751 1666 1384 268 1917
Fragments arranged by size:
1917 1666 1384 751 268
AfeI AGC'GCT
Cuts at: 2255 2255
Size: 5986
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 2517 4885 5862 1203
Size: 1314 2368 977 1327
Fragments arranged by size:
2368 1327 1314 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 2089 2275 3729 5301 2089
Size: 186 1454 1572 2774
Fragments arranged by size:
2774 1572 1454 186
ApaI G_GGCC'C
Cuts at: 2554 2554
Size: 5986
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 3295 3521 3673 5782 1493
Size: 1802 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2319 2361 2609 3092 3311 1870
Size: 73 376 42 248 483 219 4545
Fragments arranged by size:
4545 483 376 248 219 73 42
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 2460 2575 3189 3218
Size: 325 217 627 539 134 115 614 29
Cuts at: 3218 3307 4762 618
Size: 89 1455 1842
Fragments arranged by size:
1842 1455 627 614 539 325 217 134
115 89 29
BanII G_rGCy'C
Cuts at: 1391 2554 2568 2845 3305 1391
Size: 1163 14 277 460 4072
Fragments arranged by size:
4072 1163 460 277 14
BbeI G_GCGC'C
Cuts at: 3222 3222
Size: 5986
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2324 3129 4597
Size: 107 122 71 631 605 652 805 1468
Cuts at: 4597 136
Size: 1525
Fragments arranged by size:
1525 1468 805 652 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 2268 3227 3403 2268
Size: 959 176 4851
Fragments arranged by size:
4851 959 176
Bme1580I G_kGCm'C
Cuts at: 1790 2554 2580 4047 5203 1790
Size: 764 26 1467 1156 2573
Fragments arranged by size:
2573 1467 1156 764 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2512 2537 3717
Size: 416 53 83 399 24 1095 25 1180
Cuts at: 3717 4675 442
Size: 958 1753
Fragments arranged by size:
1753 1180 1095 958 416 399 83 53
25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2528 2732 2972 4646 1239
Size: 1289 204 240 1674 2579
Fragments arranged by size:
2579 1674 1289 240 204
Bpu10I CC'TnA_GC
Cuts at: 3119 3119
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 4111 4976 5274 2174
Size: 1937 865 298 2886
Fragments arranged by size:
2886 1937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2021 4649 882
Size: 617 522 2628 2219
Fragments arranged by size:
2628 2219 617 522
BsaAI yAC'GTr
Cuts at: 493 1204 2154 2686 2848 3181 3777 493
Size: 711 950 532 162 333 596 2702
Fragments arranged by size:
2702 950 711 596 532 333 162
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3219 3793 4175 4810
Size: 53 83 186 936 1686 574 382 635
Cuts at: 4810 275
Size: 1451
Fragments arranged by size:
1686 1451 936 635 574 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2882 4419 4817 5091 5238 5649 2882
Size: 1537 398 274 147 411 3219
Fragments arranged by size:
3219 1537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2504 2534 2705 2735 1178
Size: 30 165 30 1101 30 171 30 4429
Fragments arranged by size:
4429 1101 171 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2639 3221 1186
Size: 1453 582 3951
Fragments arranged by size:
3951 1453 582
BseYI C'CCAG_C
Cuts at: 2020 2538 2650 2946 3214 5189 5685 2020
Size: 518 112 296 268 1975 496 2321
Fragments arranged by size:
2321 1975 518 496 296 268 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 2528 2708 1163
Size: 573 669 123 180 4441
Fragments arranged by size:
4441 669 573 180 123
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2371 3436 4197 5225 1540
Size: 275 186 370 1065 761 1028 2301
Fragments arranged by size:
2301 1065 1028 761 370 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2139 4047 4132 5203 2121
Size: 18 1908 85 1071 2904
Fragments arranged by size:
2904 1908 1071 85 18
BsmI GAATG_Cn'
Cuts at: 2814 3497 3590 2814
Size: 683 93 5210
Fragments arranged by size:
5210 683 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2139 2554 2568 2580 2845 3305
Size: 399 331 18 415 14 12 265 460
Cuts at: 3305 4047 4132 5203 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 415 399 331 265
85 18 14 12
BspEI T'CCGG_A
Cuts at: 2882 5649 2882
Size: 2767 3219
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 2282 3341 844
Size: 1362 76 1059 3489
Fragments arranged by size:
3489 1362 1059 76
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2809 4481 4655 65
Size: 1183 1561 1672 174 1396
Fragments arranged by size:
1672 1561 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2532 2789 4630 4817 1700
Size: 110 722 257 1841 187 2869
Fragments arranged by size:
2869 1841 722 257 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2686 794
Size: 1892 4094
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 2950 2950
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 3316 3683 4068 4085 5537 5623 5635 5981
Size: 1344 367 385 17 1452 86 12 346
Cuts at: 5981 1972
Size: 1977
Fragments arranged by size:
1977 1452 1344 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2418 2568 2899 3185 513
Size: 552 1353 150 331 286 3314
Fragments arranged by size:
3314 1353 552 331 286 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 3502 4296 4316 3502
Size: 794 20 5172
Fragments arranged by size:
5172 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DraIII CAC_nnn'GTG
Cuts at: 2506 2984 2506
Size: 478 5508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 2368 2421
Size: 54 1425 50 275 18 331 207 53
Cuts at: 2421 2975 3433 4322 5951 8
Size: 554 458 889 1629 43
Fragments arranged by size:
1629 1425 889 554 458 331 275 207
54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2368 3433 1537
Size: 275 556 1065 4090
Fragments arranged by size:
4090 1065 556 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3053 3916 1353
Size: 1700 863 3423
Fragments arranged by size:
3423 1700 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 3065 4573 4947 5093 387
Size: 1064 138 660 816 1508 374 146 1280
Fragments arranged by size:
1508 1280 1064 816 660 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2383 2528 2732 2972 4049 4646 5433
Size: 393 751 145 204 240 1077 597 787
Cuts at: 5433 5701 1239
Size: 268 1524
Fragments arranged by size:
1524 1077 787 751 597 393 268 240
204 145
EcoNI CCTnn'n_nnAGG
Cuts at: 3165 3165
Size: 5986
EcoO109I rG'GnC_Cy
Cuts at: 2550 2551 2890 3028 3158 2550
Size: 1 339 138 130 5378
Fragments arranged by size:
5378 339 138 130 1
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 5986
FseI GG_CCGG'CC
Cuts at: 2536 2536
Size: 5986
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 2257 3222 5133 1678
Size: 579 965 1911 2531
Fragments arranged by size:
2531 1911 965 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2215 2247 2473
Size: 32 135 32 427 32 184 32 226
Cuts at: 2473 2504 2505 2536 2705 2737 4627 4659 4701
Size: 31 1 31 169 32 1890 32 42
Cuts at: 4701 4733 1373
Size: 32 2626
Fragments arranged by size:
2626 1890 427 226 184 169 135 42
32 32 32 32 32 32 32 31
31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KasI G'GCGC_C
Cuts at: 3218 3218
Size: 5986
KpnI G_GTAC'C
Cuts at: 2464 3311 2464
Size: 847 5139
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 2517 5862 2517
Size: 3345 2641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2423 2977 10
Size: 54 1425 343 591 554 3019
Fragments arranged by size:
3019 1425 591 554 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 2573 3944 4303 4462 518
Size: 648 1257 150 1371 359 159 2042
Fragments arranged by size:
2042 1371 1257 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2950 3187 3402 4079 5227 5472 1606
Size: 1344 237 215 677 1148 245 2120
Fragments arranged by size:
2120 1344 1148 677 245 237 215
NaeI GCC'GGC
Cuts at: 2534 2791 2534
Size: 257 5729
NarI GG'CG_CC
Cuts at: 3219 3219
Size: 5986
NcoI C'CATG_G
Cuts at: 513 1065 2418 2568 513
Size: 552 1353 150 3931
Fragments arranged by size:
3931 1353 552 150
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NgoMIV G'CCGG_C
Cuts at: 2532 2789 2532
Size: 257 5729
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NruI TCG'CGA
Cuts at: 3087 3087
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 3344 4889 1132
Size: 97 2115 1545 2229
Fragments arranged by size:
2229 2115 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2782 1108
Size: 1674 4312
PfoI T'CCnGG_A
Cuts at: 2919 2919
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PmlI CAC'GTG
Cuts at: 2154 2686 2848 3181 2154
Size: 532 162 333 4959
Fragments arranged by size:
4959 532 333 162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2890 3028 3158 2890
Size: 138 130 5718
Fragments arranged by size:
5718 138 130
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PspOMI G'GGCC_C
Cuts at: 2550 2550
Size: 5986
PstI C_TGCA'G
Cuts at: 830 2061 2208 2682 3338 830
Size: 1231 147 474 656 3478
Fragments arranged by size:
3478 1231 656 474 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2950 2950
Size: 5986
SacII CC_GC'GG
Cuts at: 3188 3188
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SanDI GG'GwC_CC
Cuts at: 2890 3028 2890
Size: 138 5848
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SexAI A'CCwGG_T
Cuts at: 2595 2595
Size: 5986
SfcI C'TryA_G
Cuts at: 826 1045 2057 2204 2678 3334 4469 5150 5341
Size: 219 1012 147 474 656 1135 681 191
Cuts at: 5341 826
Size: 1471
Fragments arranged by size:
1471 1135 1012 681 656 474 219 191
147
SfoI GGC'GCC
Cuts at: 3220 3220
Size: 5986
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 2321 3313 1872
Size: 449 992 4545
Fragments arranged by size:
4545 992 449
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 4090 4991 5253 820
Size: 197 926 246 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 2063 2907 1517
Size: 546 844 4596
Fragments arranged by size:
4596 844 546
StyI C'CwwG_G
Cuts at: 513 1065 2158 2418 2568 3425 513
Size: 552 1093 260 150 857 3074
Fragments arranged by size:
3074 1093 857 552 260 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2494 2960 4013 4030 4183 4368 4813
Size: 1255 914 466 1053 17 153 185 445
Cuts at: 4813 325
Size: 1498
Fragments arranged by size:
1498 1255 1053 914 466 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3066 4232 5944
Size: 274 80 33 51 1443 1089 1166 1712
Cuts at: 5944 96
Size: 138
Fragments arranged by size:
1712 1443 1166 1089 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2691 2723 5946 5978 2691
Size: 32 3223 32 2699
Fragments arranged by size:
3223 2699 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 2319 3311 1870
Size: 449 992 4545
Fragments arranged by size:
4545 992 449
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FspAI NsiI PacI
PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI
XcmI