Restriction map for
pFN21AB6672
(Circular) (Six-base) MAPSORT of: pFN21AB6672.seq Check: 5941 from: 1 to: 6067
pFN21AB6672 6067 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 4130 5514 5782 1632
Size: 675 1823 1384 268 1917
Fragments arranged by size:
1917 1823 1384 675 268
AflII C'TTAA_G
Cuts at: 820 1017 2027 820
Size: 197 1010 4860
Fragments arranged by size:
4860 1010 197
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 2591 3217 3810 5382 2591
Size: 626 593 1572 3276
Fragments arranged by size:
3276 1572 626 593
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 2166 2540 2562 2594 3376 3602 3754 5863
Size: 673 374 22 32 782 226 152 2109
Cuts at: 5863 1493
Size: 1697
Fragments arranged by size:
2109 1697 782 673 374 226 152 32
22
AseI AT'TA_AT
Cuts at: 160 1032 2787 4621 160
Size: 872 1755 1834 1606
Fragments arranged by size:
1834 1755 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 3392 1870
Size: 73 1449 4545
Fragments arranged by size:
4545 1449 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3388 4843 618
Size: 325 217 627 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3386 1391
Size: 1995 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 2881 928
Size: 971 235 747 4114
Fragments arranged by size:
4114 971 747 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4258 4292 1903
Size: 34 50 34 2237 34 3678
Fragments arranged by size:
3678 2237 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2342 2382 3125 3959 5169 2342
Size: 40 743 834 1210 3240
Fragments arranged by size:
3240 1210 834 743 40
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2695 6062 2695
Size: 3367 2700
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 4128 5284 1790
Size: 2338 1156 2573
Fragments arranged by size:
2573 2338 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2442 2442
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2095 2995 4727 1239
Size: 832 24 900 1732 2579
Fragments arranged by size:
2579 1732 900 832 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4192 5057 5355 4192
Size: 865 298 4904
Fragments arranged by size:
4904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2321 4730 882
Size: 617 822 2409 2219
Fragments arranged by size:
2409 2219 822 617
BsaAI yAC'GTr
Cuts at: 493 1204 3244 3858 493
Size: 711 2040 614 2702
Fragments arranged by size:
2702 2040 711 614
BsaBI GATnn'nnATC
Cuts at: 2694 6061 2694
Size: 3367 2700
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3874 4256 4891 275
Size: 53 83 186 936 2341 382 635 1451
Fragments arranged by size:
2341 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2598 2613 4500 4898 5172 5319 5730 2598
Size: 15 1887 398 274 147 411 2935
Fragments arranged by size:
2935 1887 411 398 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2565 2595 1178
Size: 30 165 30 1162 30 4650
Fragments arranged by size:
4650 1162 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2394 2415 2998 1186
Size: 1208 21 583 4255
Fragments arranged by size:
4255 1208 583 21
BseYI C'CCAG_C
Cuts at: 2058 2353 5270 5766 2058
Size: 295 2917 496 2359
Fragments arranged by size:
2917 2359 496 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2217 2841 1163
Size: 573 481 624 4389
Fragments arranged by size:
4389 624 573 481
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3265 4128 4213 5284 3265
Size: 863 85 1071 4048
Fragments arranged by size:
4048 1071 863 85
BsmI GAATG_Cn'
Cuts at: 3193 3578 3671 3193
Size: 385 93 5589
Fragments arranged by size:
5589 385 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3265 3386 4128 4213 5284 1391
Size: 399 1475 121 742 85 1071 2174
Fragments arranged by size:
2174 1475 1071 742 399 121 85
BspEI T'CCGG_A
Cuts at: 2598 5730 2598
Size: 3132 2935
BspHI T'CATG_A
Cuts at: 2475 3956 5686 2475
Size: 1481 1730 2856
Fragments arranged by size:
2856 1730 1481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3422 844
Size: 2578 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3954 1394
Size: 429 2131 3507
Fragments arranged by size:
3507 2131 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6067
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 2251 2695 3094 3397 3764 4149 4166 5618
Size: 279 444 399 303 367 385 17 1452
Cuts at: 5618 5704 5716 6062 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 444 399 385 367 346 303
279 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5515
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 3583 4377 4397 2586
Size: 997 794 20 4256
Fragments arranged by size:
4256 997 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032
Size: 54 1425 50 275 18 1684 889 1629
Cuts at: 6032 8
Size: 43
Fragments arranged by size:
1684 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 2319 3997 1353
Size: 930 36 1678 3423
Fragments arranged by size:
3423 1678 930 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2095 2307 2995 4130 4727 5514
Size: 393 439 24 212 688 1135 597 787
Cuts at: 5514 5782 1239
Size: 268 1524
Fragments arranged by size:
1524 1135 787 688 597 439 393 268
212 24
EcoO109I rG'GnC_Cy
Cuts at: 2882 2882
Size: 6067
EcoRI G'AATT_C
Cuts at: 2594 3376 3754 2594
Size: 782 378 4907
Fragments arranged by size:
4907 782 378
EcoRV GAT'ATC
Cuts at: 2758 2758
Size: 6067
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 5214 1678
Size: 3536 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2389 2421 2758 2790 2829
Size: 32 135 32 817 32 337 32 39
Cuts at: 2829 2861 3293 3325 4708 4740 4782 4814 1373
Size: 32 432 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 817 432 337 135 42 39
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2488 3411 3657 669
Size: 988 281 550 923 246 3079
Fragments arranged by size:
3079 988 923 550 281 246
HindIII A'AGCT_T
Cuts at: 748 2727 748
Size: 1979 4088
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 3007 3290 5181 5365 1305
Size: 969 733 283 1891 184 2007
Fragments arranged by size:
2007 1891 969 733 283 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 3186 4025 4384 4543 518
Size: 648 1308 712 839 359 159 2042
Fragments arranged by size:
2042 1308 839 712 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2591 3483 4160 5308 5553 1606
Size: 985 892 677 1148 245 2120
Fragments arranged by size:
2120 1148 985 892 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5515
NdeI CA'TA_TG
Cuts at: 387 3183 387
Size: 2796 3271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3425 4970 1132
Size: 97 2196 1545 2229
Fragments arranged by size:
2229 2196 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PfoI T'CCnGG_A
Cuts at: 3090 3090
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2882 2882
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 2887 1613
Size: 1274 4793
PsiI TTA'TAA
Cuts at: 3036 3637 3036
Size: 601 5466
PstI C_TGCA'G
Cuts at: 830 3419 830
Size: 2589 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2591 2591
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 2300 4315 6027 2300
Size: 2015 1712 2340
Fragments arranged by size:
2340 2015 1712
SfcI C'TryA_G
Cuts at: 826 1045 3204 3415 4550 5231 5422 826
Size: 219 2159 211 1135 681 191 1471
Fragments arranged by size:
2159 1471 1135 681 219 211 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 4171 5072 5334 820
Size: 197 926 84 2144 901 262 1553
Fragments arranged by size:
2144 1553 926 901 262 197 84
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2316 3506 513
Size: 552 1251 1190 3074
Fragments arranged by size:
3074 1251 1190 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2429 2770 4094 4111 4264 4449 4894
Size: 1255 849 341 1324 17 153 185 445
Cuts at: 4894 325
Size: 1498
Fragments arranged by size:
1498 1324 1255 849 445 341 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 3294 4313
Size: 274 80 33 51 1443 321 996 1019
Cuts at: 4313 6025 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1019 996 321 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2404 2436 2565 2597 6027 6059 2404
Size: 32 129 32 3430 32 2412
Fragments arranged by size:
3430 2412 129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2471 1316
Size: 1155 4912
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2567 2567
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI