Restriction map for
pFN21AB6671
(Circular) (Six-base) MAPSORT of: pFN21AB6671.seq Check: 9587 from: 1 to: 5983
pFN21AB6671 5983 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2019 3326 1937
Size: 82 1307 4594
Fragments arranged by size:
4594 1307 82
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4046 5430 5698 1632
Size: 2414 1384 268 1917
Fragments arranged by size:
2414 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 2323 2754 4882 5859 1203
Size: 1120 431 2128 977 1327
Fragments arranged by size:
2128 1327 1120 977 431
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 2123 1166
Size: 957 5026
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3169 3181 3201 3149
Size: 20 12 20 5931
Fragments arranged by size:
5931 20 20 12
AlwNI CAG_nnn'CTG
Cuts at: 3174 3726 5298 3174
Size: 552 1572 3859
Fragments arranged by size:
3859 1572 552
ApaLI G'TGCA_C
Cuts at: 2849 4040 5196 2849
Size: 1191 1156 3636
Fragments arranged by size:
3636 1191 1156
ApoI r'AATT_y
Cuts at: 1493 3292 3518 3670 5779 1493
Size: 1799 226 152 2109 1697
Fragments arranged by size:
2109 1799 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 3019 3308 1870
Size: 73 286 790 289 4545
Fragments arranged by size:
4545 790 289 286 73
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2690 2888 3304 4759 618
Size: 325 217 627 903 198 416 1455 1842
Fragments arranged by size:
1842 1455 903 627 416 325 217 198
BanII G_rGCy'C
Cuts at: 1391 3302 1391
Size: 1911 4072
BbeI G_GCGC'C
Cuts at: 2892 2892
Size: 5983
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3113 928
Size: 971 1214 3798
Fragments arranged by size:
3798 1214 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2195 2210 2229 2244 4174 4208 1903
Size: 34 258 15 19 15 1930 34 3678
Fragments arranged by size:
3678 1930 258 34 34 19 15 15
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2193 5978 2193
Size: 3785 2198
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2695 2853 3209 3281 4044 5200 1790
Size: 905 158 356 72 763 1156 2573
Fragments arranged by size:
2573 1156 905 763 356 158 72
BmgBI CAC'GTC
Cuts at: 1318 2326 1318
Size: 1008 4975
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 2345 3212
Size: 416 53 83 399 24 873 55 867
Cuts at: 3212 3714 4672 442
Size: 502 958 1753
Fragments arranged by size:
1753 958 873 867 502 416 399 83
55 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2186 4643 1239
Size: 941 6 2457 2579
Fragments arranged by size:
2579 2457 941 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 4108 4973 5271 2243
Size: 1865 865 298 2955
Fragments arranged by size:
2955 1865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2737 2806 4646 882
Size: 617 1238 69 1840 2219
Fragments arranged by size:
2219 1840 1238 617 69
BsaAI yAC'GTr
Cuts at: 493 1204 2224 2977 3774 493
Size: 711 1020 753 797 2702
Fragments arranged by size:
2702 1020 797 753 711
BsaBI GATnn'nnATC
Cuts at: 3224 5977 3224
Size: 2753 3230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 2889 3790 4172
Size: 53 83 186 936 483 873 901 382
Cuts at: 4172 4807 275
Size: 635 1451
Fragments arranged by size:
1451 936 901 873 635 483 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2045 2049 2075 2229
Size: 30 165 30 616 26 4 26 154
Cuts at: 2229 2234 2259 2264 2585 2615 3149 3179 3260
Size: 5 25 5 321 30 534 30 81
Cuts at: 3260 3290 1178
Size: 30 3871
Fragments arranged by size:
3871 616 534 321 165 154 81 30
30 30 30 30 26 26 25 5
5 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 3260 1186
Size: 973 1101 3909
Fragments arranged by size:
3909 1101 973
BseYI C'CCAG_C
Cuts at: 2078 2087 5186 5682 2078
Size: 9 3099 496 2379
Fragments arranged by size:
3099 2379 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2330 2817 1163
Size: 573 468 126 487 4329
Fragments arranged by size:
4329 573 487 468 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2358 2466 2853 4044 4129 5200 2358
Size: 108 387 1191 85 1071 3141
Fragments arranged by size:
3141 1191 1071 387 108 85
BsmI GAATG_Cn'
Cuts at: 3494 3587 3494
Size: 93 5890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2358 2466 2695 2853 3209 3281 3302
Size: 399 568 108 229 158 356 72 21
Cuts at: 3302 4044 4129 5200 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 568 399 356 229 158
108 85 72 21
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 2788 3338 844
Size: 1508 436 550 3489
Fragments arranged by size:
3489 1508 550 436
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2240 3870 1394
Size: 429 187 230 1630 3507
Fragments arranged by size:
3507 1630 429 230 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 3135 4478 4652 65
Size: 1183 1887 1343 174 1396
Fragments arranged by size:
1887 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3047 4627 4814 1700
Size: 110 1237 1580 187 2869
Fragments arranged by size:
2869 1580 1237 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 2498 4043 5055 2498
Size: 1545 1012 3426
Fragments arranged by size:
3426 1545 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2353 794
Size: 1559 4424
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 2980 1184
Size: 1796 4187
BstXI CCAn_nnnn'nTGG
Cuts at: 2125 2125
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 2193 3225 3313 3680 4065 4082 5534 5620
Size: 221 1032 88 367 385 17 1452 86
Cuts at: 5620 5632 5978 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1032 385 367 346 221 88
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2910 2910
Size: 5983
BtgI C'CryG_G
Cuts at: 513 1065 2139 2372 2526 3153 513
Size: 552 1074 233 154 627 3343
Fragments arranged by size:
3343 1074 627 552 233 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 2615 3059 3266 3499 4293 4313 2615
Size: 444 207 233 794 20 4285
Fragments arranged by size:
4285 794 444 233 207 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DraIII CAC_nnn'GTG
Cuts at: 2353 2980 2353
Size: 627 5356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2529 2634
Size: 54 1425 50 275 18 306 393 105
Cuts at: 2634 3430 4319 5948 8
Size: 796 889 1629 43
Fragments arranged by size:
1629 1425 889 796 393 306 275 105
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3913 1353
Size: 2560 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2040 4570 4944 5090 387
Size: 1064 138 451 2530 374 146 1280
Fragments arranged by size:
2530 1280 1064 451 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2186 4046 4643 5430 5698 1239
Size: 393 548 6 1860 597 787 268 1524
Fragments arranged by size:
1860 1524 787 597 548 393 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2586 2586
Size: 5983
EcoO109I rG'GnC_Cy
Cuts at: 2914 2941 2914
Size: 27 5956
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 5983
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 2045 2892 5130 1678
Size: 367 847 2238 2531
Fragments arranged by size:
2531 2238 847 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2229 2234 2261
Size: 32 135 32 473 32 152 5 27
Cuts at: 2261 2266 2585 2617 2889 2921 3117 3149 4624
Size: 5 319 32 272 32 196 32 1475
Cuts at: 4624 4656 4698 4730 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1475 473 319 272 196 152 135
42 32 32 32 32 32 32 32
32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KasI G'GCGC_C
Cuts at: 2888 2888
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5097 5281 1305
Size: 3792 184 2007
Fragments arranged by size:
3792 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2636 10
Size: 54 1425 343 804 3357
Fragments arranged by size:
3357 1425 804 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2603 2996 3941 4300 4459 518
Size: 648 957 480 393 945 359 159 2042
Fragments arranged by size:
2042 957 945 648 480 393 359 159
MspA1I CmG'CkG
Cuts at: 1606 2141 3399 4076 5224 5469 1606
Size: 535 1258 677 1148 245 2120
Fragments arranged by size:
2120 1258 1148 677 535 245
NarI GG'CG_CC
Cuts at: 2889 2889
Size: 5983
NcoI C'CATG_G
Cuts at: 513 1065 3153 513
Size: 552 2088 3343
Fragments arranged by size:
3343 2088 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2758 3001 3124 3341 4886 1132
Size: 97 1529 243 123 217 1545 2229
Fragments arranged by size:
2229 1545 1529 243 217 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5983
PciI A'CATG_T
Cuts at: 2754 4882 2754
Size: 2128 3855
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 2742 1108
Size: 1524 110 4349
Fragments arranged by size:
4349 1524 110
PfoI T'CCnGG_A
Cuts at: 2301 2625 2682 2772 2301
Size: 324 57 90 5512
Fragments arranged by size:
5512 324 90 57
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PmlI CAC'GTG
Cuts at: 2224 2977 2224
Size: 753 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2399 2431 3149 3181 4052 4084 5565 5597 2399
Size: 32 718 32 871 32 1481 32 2785
Fragments arranged by size:
2785 1481 871 718 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PstI C_TGCA'G
Cuts at: 830 2562 2799 3069 3335 830
Size: 1732 237 270 266 3478
Fragments arranged by size:
3478 1732 270 266 237
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SacII CC_GC'GG
Cuts at: 2142 2142
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2314 2558 2795 3065 3331 4466 5147
Size: 219 1269 244 237 270 266 1135 681
Cuts at: 5147 5338 826
Size: 191 1471
Fragments arranged by size:
1471 1269 1135 681 270 266 244 237
219 191
SfoI GGC'GCC
Cuts at: 2890 2890
Size: 5983
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 3047 1700
Size: 1347 4636
SmaI CCC'GGG
Cuts at: 1872 3021 3310 1872
Size: 1149 289 4545
Fragments arranged by size:
4545 1149 289
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 4087 4988 5250 820
Size: 197 926 315 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 315 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3124 3341 1132
Size: 1992 217 3774
Fragments arranged by size:
3774 1992 217
SrfI GCCC'GGGC
Cuts at: 3021 3021
Size: 5983
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 2786 1517
Size: 1269 4714
StyI C'CwwG_G
Cuts at: 513 1065 2103 2670 3153 3422 513
Size: 552 1038 567 483 269 3074
Fragments arranged by size:
3074 1038 567 552 483 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2364 2534 2954 4010 4027 4180 4365
Size: 1255 784 170 420 1056 17 153 185
Cuts at: 4365 4810 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1056 784 445 420 185 170
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2539 4229
Size: 274 80 33 51 1443 447 115 1690
Cuts at: 4229 5941 96
Size: 1712 138
Fragments arranged by size:
1712 1690 1443 447 274 138 115 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 2270 2302 2518 2550 3260 3292 5943
Size: 32 219 32 216 32 710 32 2651
Cuts at: 5943 5975 2019
Size: 32 2027
Fragments arranged by size:
2651 2027 710 219 216 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2965 2965
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3019 3308 1870
Size: 1149 289 4545
Fragments arranged by size:
4545 1149 289
XmnI GAAnn'nnTTC
Cuts at: 2195 4112 2195
Size: 1917 4066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI
SfiI SwaI